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- PLoS One
- v.6(12); 2011
- PMC3245232

# A Higher-Order Generalized Singular Value Decomposition for Comparison of Global mRNA Expression from Multiple Organisms

^{}

^{1}Department of Electrical and Computer Engineering, University of Texas at Austin, Texas, United States of America

^{2}Department of Management Science and Engineering, Stanford University, Stanford, California, United States of America

^{3}Department of Computer Science, Cornell University, Ithaca, New York, United States of America

^{4}Scientific Computing and Imaging (SCI) Institute and Departments of Bioengineering and Human Genetics, University of Utah, Salt Lake City, Utah, United States of America

Conceived and designed the experiments: OA. Performed the experiments: SPP OA. Analyzed the data: SPP OA. Contributed reagents/materials/analysis tools: SPP OA. Wrote the paper: SPP MAS CFVL OA. Proved mathematical theorems: SPP MAS CFVL OA.

## Abstract

The number of high-dimensional datasets recording multiple aspects of a single phenomenon is increasing in many areas of science, accompanied by a need for mathematical frameworks that can compare multiple large-scale matrices with different row dimensions. The only such framework to date, the generalized singular value decomposition (GSVD), is limited to two matrices. We mathematically define a higher-order GSVD (HO GSVD) for *N*≥2 matrices , each with full column rank. Each matrix is exactly factored as *D _{i}*=

*U*Σ

_{i}*, where*

_{i}V^{T}*V*, identical in all factorizations, is obtained from the eigensystem

*SV*=

*V*Λ of the arithmetic mean

*S*of all pairwise quotients of the matrices ,

*i*≠

*j*. We prove that this decomposition extends to higher orders almost all of the mathematical properties of the GSVD. The matrix

*S*is nondefective with

*V*and Λ real. Its eigenvalues satisfy

*λ*≥1. Equality holds if and only if the corresponding eigenvector

_{k}*v*is a right basis vector of equal significance in all matrices

_{k}*D*and

_{i}*D*, that is

_{j}*σ*/

_{i,k}*σ*=1 for all

_{j,k}*i*and

*j*, and the corresponding left basis vector

*u*is orthogonal to all other vectors in

_{i,k}*U*for all

_{i}*i*. The eigenvalues

*λ*=1, therefore, define the “common HO GSVD subspace.” We illustrate the HO GSVD with a comparison of genome-scale cell-cycle mRNA expression from

_{k}*S. pombe*,

*S. cerevisiae*and human. Unlike existing algorithms, a mapping among the genes of these disparate organisms is not required. We find that the approximately common HO GSVD subspace represents the cell-cycle mRNA expression oscillations, which are similar among the datasets. Simultaneous reconstruction in the common subspace, therefore, removes the experimental artifacts, which are dissimilar, from the datasets. In the simultaneous sequence-independent classification of the genes of the three organisms in this common subspace, genes of highly conserved sequences but significantly different cell-cycle peak times are correctly classified.

## Introduction

In many areas of science, especially in biotechnology, the number of high-dimensional datasets recording multiple aspects of a single phenomenon is increasing. This is accompanied by a fundamental need for mathematical frameworks that can compare multiple large-scale matrices with different row dimensions. For example, comparative analyses of global mRNA expression from multiple model organisms promise to enhance fundamental understanding of the universality and specialization of molecular biological mechanisms, and may prove useful in medical diagnosis, treatment and drug design [1]. Existing algorithms limit analyses to subsets of homologous genes among the different organisms, effectively introducing into the analysis the assumption that sequence and functional similarities are equivalent (e.g., [2]). However, it is well known that this assumption does not always hold, for example, in cases of nonorthologous gene displacement, when nonorthologous proteins in different organisms fulfill the same function [3]. For sequence-independent comparisons, mathematical frameworks are required that can distinguish and separate the similar from the dissimilar among multiple large-scale datasets tabulated as matrices with different row dimensions, corresponding to the different sets of genes of the different organisms. The only such framework to date, the generalized singular value decomposition (GSVD) [4]–[7], is limited to two matrices.

It was shown that the GSVD provides a mathematical framework for sequence-independent comparative modeling of DNA microarray data from two organisms, where the mathematical variables and operations represent biological reality [7], [8]. The variables, significant subspaces that are common to both or exclusive to either one of the datasets, correlate with cellular programs that are conserved in both or unique to either one of the organisms, respectively. The operation of reconstruction in the subspaces common to both datasets outlines the biological similarity in the regulation of the cellular programs that are conserved across the species. Reconstruction in the common and exclusive subspaces of either dataset outlines the differential regulation of the conserved relative to the unique programs in the corresponding organism. Recent experimental results [9] verify a computationally predicted genome-wide mode of regulation that correlates DNA replication origin activity with mRNA expression [10], [11], demonstrating that GSVD modeling of DNA microarray data can be used to correctly predict previously unknown cellular mechanisms.

We now define a higher-order GSVD (HO GSVD) for the comparison of datasets. The datasets are tabulated as real matrices , each with full column rank, with different row dimensions and the same column dimension, where there exists a one-to-one mapping among the columns of the matrices. Like the GSVD, the HO GSVD is an exact decomposition, i.e., each matrix is exactly factored as , where the columns of and have unit length and are the left and right basis vectors respectively, and each is diagonal and positive definite. Like the GSVD, the matrix is identical in all factorizations. In our HO GSVD, the matrix is obtained from the eigensystem of the arithmetic mean of all pairwise quotients of the matrices , or equivalently of all , .

To clarify our choice of , we note that in the GSVD, defined by Van Loan [5], the matrix can be formed from the eigenvectors of the unbalanced quotient (Section 1 in Appendix S1). We observe that this can also be formed from the eigenvectors of the balanced arithmetic mean . We prove that in the case of , our definition of by using the eigensystem of leads algebraically to the GSVD (Theorems S1–S5 in Appendix S1), and therefore, as Paige and Saunders showed [6], can be computed in a stable way. We also note that in the GSVD, the matrix does not depend upon the ordering of the matrices and . Therefore, we define our HO GSVD for matrices by using the balanced arithmetic mean of all pairwise arithmetic means , each of which defines the GSVD of the corresponding pair of matrices and , noting that does not depend upon the ordering of the matrices and .

We prove that is nondefective (it has independent eigenvectors), and that its eigensystem is real (Theorem 1). We prove that the eigenvalues of satisfy (Theorem 2). As in our GSVD comparison of two matrices [7], we interpret the th diagonal of in the factorization of the th matrix as indicating the significance of the th right basis vector in in terms of the overall information that captures in . The ratio indicates the significance of in relative to its significance in . We prove that an eigenvalue of satisfies if and only if the corresponding eigenvector is a right basis vector of equal significance in all and , that is, for all and , and the corresponding left basis vector is orthonormal to all other vectors in for all . We therefore mathematically define, in analogy with the GSVD, the “common HO GSVD subspace” of the matrices to be the subspace spanned by the right basis vectors that correspond to the eigenvalues of (Theorem 3). We also show that each of the right basis vectors that span the common HO GSVD subspace is a generalized singular vector of all pairwise GSVD factorizations of the matrices and with equal corresponding generalized singular values for all and (Corollary 1).

Recent research showed that several higher-order generalizations are possible for a given matrix decomposition, each preserving some but not all of the properties of the matrix decomposition [12]–[14] (see also Theorem S6 and Conjecture S1 in Appendix S1). Our new HO GSVD extends to higher orders all of the mathematical properties of the GSVD except for complete column-wise orthogonality of the left basis vectors that form the matrix for all , i.e., in each factorization.

We illustrate the HO GSVD with a comparison of cell-cycle mRNA expression from *S. pombe* [15], [16], *S. cerevisiae* [17] and human [18]. Unlike existing algorithms, a mapping among the genes of these disparate organisms is not required (Section 2 in Appendix S1). We find that the common HO GSVD subspace represents the cell-cycle mRNA expression oscillations, which are similar among the datasets. Simultaneous reconstruction in this common subspace, therefore, removes the experimental artifacts, which are dissimilar, from the datasets. Simultaneous sequence-independent classification of the genes of the three organisms in the common subspace is in agreement with previous classifications into cell-cycle phases [19]. Notably, genes of highly conserved sequences across the three organisms [20], [21] but significantly different cell-cycle peak times, such as genes from the ABC transporter superfamily [22]–[28], phospholipase B-encoding genes [29], [30] and even the B cyclin-encoding genes [31], [32], are correctly classified.

## Methods

### HO GSVD Construction

Suppose we have a set of real matrices each with full column rank. We define a HO GSVD of these matrices as

where each is composed of normalized left basis vectors, each is diagonal with , and , identical in all matrix factorizations, is composed of normalized right basis vectors. As in the GSVD comparison of global mRNA expression from two organisms [7], in the HO GSVD comparison of global mRNA expression from organisms, the shared right basis vectors of Equation (1) are the “genelets” and the sets of left basis vectors are the sets of “arraylets” (Figure 1 and Section 2 in Appendix S1). We obtain from the eigensystem of , the arithmetic mean of all pairwise quotients of the matrices , or equivalently of all , :

with . We prove that is nondefective, i.e., has independent eigenvectors, and that its eigenvectors and eigenvalues are real (Theorem 1). We prove that the eigenvalues of satisfy (Theorem 2).

Given , we compute matrices by solving linear systems:

and we construct and by normalizing the columns of :

### HO GSVD Interpretation

In this construction, the rows of each of the matrices are superpositions of the same right basis vectors, the columns of (Figures S1 and S2 and Section 1 in Appendix S1). As in our GSVD comparison of two matrices, we interpret the th diagonals of , the “higher-order generalized singular value set” , as indicating the significance of the th right basis vector in the matrices , and reflecting the overall information that captures in each respectively. The ratio indicates the significance of in relative to its significance in . A ratio of for all and corresponds to a right basis vector of equal significance in all matrices . GSVD comparisons of two matrices showed that right basis vectors of approximately equal significance in the two matrices reflect themes that are common to both matrices under comparison [7]. A ratio of indicates a basis vector of almost negligible significance in relative to its significance in . GSVD comparisons of two matrices showed that right basis vectors of negligible significance in one matrix reflect themes that are exclusive to the other matrix.

We prove that an eigenvalue of satisfies if and only if the corresponding eigenvector is a right basis vector of equal significance in all and , that is, for all and , and the corresponding left basis vector is orthonormal to all other vectors in for all . We therefore mathematically define, in analogy with the GSVD, the “common HO GSVD subspace” of the matrices to be the subspace spanned by the right basis vectors corresponding to the eigenvalues of that satisfy (Theorem 3).

It follows that each of the right basis vectors that span the common HO GSVD subspace is a generalized singular vector of all pairwise GSVD factorizations of the matrices and with equal corresponding generalized singular values for all and (Corollary 1). Since the GSVD can be computed in a stable way [6], we note that the common HO GSVD subspace can also be computed in a stable way by computing all pairwise GSVD factorizations of the matrices and . This also suggests that it may be possible to formulate the HO GSVD as a solution to an optimization problem, in analogy with existing variational formulations of the GSVD [33]. Such a formulation may lead to a stable numerical algorithm for computing the HO GSVD, and possibly also to a higher-order general Gauss-Markov linear statistical model [34]–[36].

We show, in a comparison of matrices, that the approximately common HO GSVD subspace of these three matrices reflects a theme that is common to the three matrices under comparison (Section 2).

### HO GSVD Mathematical Properties

#### Theorem 1

*is nondefective (it has ** independent eigenvectors) and its eigensystem is real.*

*Proof.* From Equation (2) it follows that

and the eigenvectors of equal the eigenvectors of .

Let the SVD of the matrices appended along the -columns axis be

Since the matrices are real and with full column rank, it follows from the SVD of that the symmetric matrices are real and positive definite, and their inverses exist. It then follows from Equations (5) and (6) that is similar to ,

and the eigenvalues of equal the eigenvalues of .

A sum of real, symmetric and positive definite matrices, is also real, symmetric and positive definite; therefore, its eigensystem

is real with orthogonal and . Without loss of generality let be orthonormal, such that . It follows from the similarity of with that the eigensystem of can be written as , with the real and nonsingular , where and such that for all .

Thus, from Equation (5), is nondefective with real eigenvectors . Also, the eigenvalues of satisfy

where are the eigenvalues of and . Thus, the eigenvalues of are real. □

#### Theorem 2

*The eigenvalues of ** satisfy **.*

*Proof.* Following Equation (9), asserting that the eigenvalues of satisfy is equivalent to asserting that the eigenvalues of satisfy .

From Equations (6) and (7), the eigenvalues of satisfy

under the constraint that

where is a real unit vector, and where it follows from the Cauchy-Schwarz inequality [37] (see also [4], [34], [38]) for the real nonzero vectors and that for all

With the constraint of Equation (11), which requires the sum of the positive numbers to equal one, the lower bound on the eigenvalues of in Equation (10) is at its minimum when the sum of the inverses of these numbers is at its minimum, that is, when the numbers equal

for all and . Thus, the eigenvalues of satisfy . □

#### Theorem 3

*The common HO GSVD subspace.** An eigenvalue of ** satisfies ** if and only if the corresponding eigenvector ** is a right basis vector of equal significance in all ** and **, that is, ** for all ** and **, and the corresponding left basis vector ** is orthonormal to all other vectors in ** for all **. The “common HO GSVD subspace” of the ** matrices is, therefore, the subspace spanned by the right basis vectors ** corresponding to the eigenvalues of ** that satisfy **.*

*Proof.* Without loss of generality, let . From Equation (12) and the Cauchy-Schwarz inequality, an eigenvalue of equals its minimum lower bound if and only if the corresponding eigenvector is also an eigenvector of for all [37], where, from Equation (13), the corresponding eigenvalue equals ,

Given the eigenvectors of , we solve Equation (3) for each of Equation (6), and obtain

Following Equations (14) and (15), where corresponds to a minimum eigenvalue , and since is orthonormal, we obtain

with zeroes in the th row and the th column of the matrix above everywhere except for the diagonal element. Thus, an eigenvalue of satisfies if and only if the corresponding left basis vectors are orthonormal to all other vectors in .

The corresponding higher-order generalized singular values are . Thus for all and , and the corresponding right basis vector is of equal significance in all matrices and . □

#### Corollary 1

*An eigenvalue of ** satisfies ** if and only if the corresponding right basis vector ** is a generalized singular vector of all pairwise GSVD factorizations of the matrices ** and ** with equal corresponding generalized singular values for all ** and **.*

*Proof.* From Equations (12) and (13), and since the pairwise quotients are similar to with the similarity transformation of for all and , it follows that an eigenvalue of satisfies if and only if the corresponding right basis vector is also an eigenvector of each of the pairwise quotients of the matrices with equal corresponding eigenvalues, or equivalently of all with all eigenvalues at their minimum of one,

We prove (Theorems S1–S5 in Appendix S1) that in the case of matrices our definition of by using the eigensystem of leads algebraically to the GSVD, where an eigenvalue of equals its minimum of one if and only if the two corresponding generalized singular values are equal, such that the corresponding generalized singular vector is of equal significance in both matrices and . Thus, it follows that each of the right basis vectors that span the common HO GSVD subspace is a generalized singular vector of all pairwise GSVD factorizations of the matrices and with equal corresponding generalized singular values for all and . □

Note that since the GSVD can be computed in a stable way [6], the common HO GSVD subspace we define (Theorem 3) can also be computed in a stable way by computing all pairwise GSVD factorizations of the matrices and (Corollary 1). It may also be possible to formulate the HO GSVD as a solution to an optimization problem, in analogy with existing variational formulations of the GSVD [33]. Such a formulation may lead to a stable numerical algorithm for computing the HO GSVD, and possibly also to a higher-order general Gauss-Markov linear statistical model [34]–[36].

## Results

### HO GSVD Comparison of Global mRNA Expression from Three Organisms

Consider now the HO GSVD comparative analysis of global mRNA expression datasets from the organisms *S. pombe*, *S. cerevisiae* and human (Section 2.1 in Appendix S1, Mathematica Notebooks S1 and S2, and Datasets S1, S2 and S3). The datasets are tabulated as matrices of columns each, corresponding to DNA microarray-measured mRNA expression from each organism at time points equally spaced during approximately two cell-cycle periods. The underlying assumption is that there exists a one-to-one mapping among the 17 columns of the three matrices but not necessarily among their rows, which correspond to either -*S. pombe* genes, -*S. cerevisiae* genes or -human genes. The HO GSVD of Equation (1) transforms the datasets from the organism-specific genes-arrays spaces to the reduced spaces of the 17-“arraylets,” i.e., left basis vactors17-“genelets,” i.e., right basis vectors, where the datasets are represented by the diagonal nonnegative matrices , by using the organism-specific genes17-arraylets transformation matrices and the one shared 17-genelets17-arrays transformation matrix (Figure 1).

Following Theorem 3, the approximately common HO GSVD subspace of the three datasets is spanned by the five genelets that correspond to . We find that these five genelets are approximately equally significant with in the *S. pombe*, *S. cerevisiae* and human datasets, respectively (Figure 2 *a* and *b*). The five corresponding arraylets in each dataset are -orthonormal to all other arraylets (Figure S3 in Appendix S1).

### Common HO GSVD Subspace Represents Similar Cell-Cycle Oscillations

The expression variations across time of the five genelets that span the approximately common HO GSVD subspace fit normalized cosine functions of two periods, superimposed on time-invariant expression (Figure 2 *c* and *d*). Consistently, the corresponding organism-specific arraylets are enriched [39] in overexpressed or underexpressed organism-specific cell cycle-regulated genes, with 24 of the 30 *P*-values (Table 1 and Section 2.2 in Appendix S1). For example, the three 17th arraylets, which correspond to the 0-phase 17th genelet, are enriched in overexpressed G2 *S. pombe* genes, G2/M and M/G1 *S. cerevisiae* genes and S and G2 human genes, respectively, representing the cell-cycle checkpoints in which the three cultures are initially synchronized.

Simultaneous sequence-independent reconstruction and classification of the three datasets in the common subspace outline cell-cycle progression in time and across the genes in the three organisms (Sections 2.3 and 2.4 in Appendix S1). Projecting the expression of the 17 arrays of either organism from the corresponding five-dimensional arraylets subspace onto the two-dimensional subspace that approximates it (Figure S4 in Appendix S1), of the contributions of the arraylets add up, rather than cancel out (Figure 3 *a*–*c*). In these two-dimensional subspaces, the angular order of the arrays of either organism describes cell-cycle progression in time through approximately two cell-cycle periods, from the initial cell-cycle phase and back to that initial phase twice. Projecting the expression of the genes, of the contributions of the five genelets add up in the overall expression of 343 of the 380 *S. pombe* genes classified as cell cycle-regulated, 554 of the 641 *S. cerevisiae* cell-cycle genes, and 632 of the 787 human cell-cycle genes (Figure 3 *d*–*f*). Simultaneous classification of the genes of either organism into cell-cycle phases according to their angular order in these two-dimensional subspaces is consistent with the classification of the arrays, and is in good agreement with the previous classifications of the genes (Figure 3 *g*–*i*). With all 3167 *S. pombe*, 4772 *S. cerevisiae* and 13,068 human genes sorted, the expression variations of the five arraylets from each organism approximately fit one-period cosines, with the initial phase of each arraylet (Figures S5, S6, S7 in Appendix S1) similar to that of its corresponding genelet (Figure 2). The global mRNA expression of each organism, reconstructed in the common HO GSVD subspace, approximately fits a traveling wave, oscillating across time and across the genes.

Note also that simultaneous reconstruction in the common HO GSVD subspace removes the experimental artifacts and batch effects, which are dissimilar, from the three datasets. Consider, for example, the second genelet. With in the *S. pombe*, *S. cerevisiae* and human datasets, respectively, this genelet is almost exclusive to the *S. cerevisiae* dataset. This genelet is anticorrelated with a time decaying pattern of expression (Figure 2*a*). Consistently, the corresponding *S. cerevisiae*-specific arraylet is enriched in underexpressed *S. cerevisiae* genes that were classified as up-regulated by the *S. cerevisiae* synchronizing agent, the -factor pheromone, with the *P*-value . Reconstruction in the common subspace effectively removes this *S. cerevisiae*-approximately exclusive pattern of expression variation from the three datasets.

### Simultaneous HO GSVD Classification of Homologous Genes of Different Cell-Cycle Peak Times

Notably, in the simultaneous sequence-independent classification of the genes of the three organisms in the common subspace, genes of significantly different cell-cycle peak times [19] but highly conserved sequences [20], [21] are correctly classified (Section 2.5 in Appendix S1).

For example, consider the G2 *S. pombe* gene *BFR1* (Figure 4*a*), which belongs to the evolutionarily highly conserved ATP-binding cassette (ABC) transporter superfamily [22]. The closest homologs of *BFR1* in our *S. pombe*, *S. cerevisiae* and human datasets are the *S. cerevisiae* genes *SNQ2*, *PDR5*, *PDR15* and *PDR10* (Table S1*a* in Appendix S1). The expression of *SNQ2* and *PDR5* is known to peak at the S/G2 and G2/M cell-cycle phases, respectively [17]. However, sequence similarity does not imply similar cell-cycle peak times, and *PDR15* and *PDR10*, the closest homologs of *PDR5*, are induced during stationary phase [23], which has been hypothesized to occur in G1, before the Cdc28-defined cell-cycle arrest [24]. Consistently, we find *PDR15* and *PDR10* at the M/G1 to G1 transition, antipodal to (i.e., half a cell-cycle period apart from) *SNQ2* and *PDR5*, which are projected onto S/G2 and G2/M, respectively (Figure 4*b*). We also find the transcription factor *PDR1* at S/G2, its known cell-cycle peak time, adjacent to *SNQ2* and *PDR5*, which it positively regulates and might be regulated by, and antipodal to *PDR15*, which it negatively regulates [25]–[28].

Another example is the *S. cerevisiae* phospholipase B-encoding gene *PLB1* [29], which peaks at the cell-cycle phase M/G1 [30]. Its closest homolog in our *S. cerevisiae* dataset, *PLB3*, also peaks at M/G1 [17] (Figure 4*d*). However, among the closest *S. pombe* and human homologs of *PLB1* (Table S1*b* in Appendix S1), we find the *S. pombe* genes *SPAC977.09c* and *SPAC1786.02*, which expressions peak at the almost antipodal *S. pombe* cell-cycle phases S and G2, respectively [19] (Figure 4*c*).

As a third example, consider the *S. pombe* G1 B-type cyclin-encoding gene *CIG2* [31], [32] (Table S1*c* in Appendix S1). Its closest *S. pombe* homolog, *CDC13*, peaks at M [19] (Figure 4*e*). The closest human homologs of *CIG2*, the cyclins *CCNA2* and *CCNB2*, peak at G2 and G2/M, respectively (Figure 4*g*). However, while periodicity in mRNA abundance levels through the cell cycle is highly conserved among members of the cyclin family, the cell-cycle peak times are not necessarily conserved [1]: The closest homologs of *CIG2* in our *S. cerevisiae* dataset, are the G2/M promoter-encoding genes *CLB1,2* and *CLB3,4*, which expressions peak at G2/M and S respectively, and *CLB5*, which encodes a DNA synthesis promoter, and peaks at G1 (Figure 4*f*).

## Discussion

We mathematically defined a higher-order GSVD (HO GSVD) for two or more large-scale matrices with different row dimensions and the same column dimension. We proved that our new HO GSVD extends to higher orders almost all of the mathematical properties of the GSVD: The eigenvalues of are always greater than or equal to one, and an eigenvalue of one corresponds to a right basis vector of equal significance in all matrices, and to a left basis vector in each matrix factorization that is orthogonal to all other left basis vectors in that factorization. We therefore mathematically defined, in analogy with the GSVD, the common HO GSVD subspace of the matrices to be the subspace spanned by the right basis vectors that correspond to the eigenvalues of that equal one.

The only property that does not extend to higher orders in general is the complete column-wise orthogonality of the normalized left basis vectors in each factorization. Recent research showed that several higher-order generalizations are possible for a given matrix decomposition, each preserving some but not all of the properties of the matrix decomposition [12]–[14]. The HO GSVD has the interesting property of preserving the exactness and diagonality of the matrix GSVD and, in special cases, also partial or even complete column-wise orthogonality. That is, all matrix factorizations in Equation (1) are exact, all matrices are diagonal, and when one or more of the eigenvalues of equal one, the corresponding left basis vectors in each factorization are orthogonal to all other left basis vectors in that factorization.

The complete column-wise orthogonality of the matrix GSVD [5] enables its stable computation [6]. We showed that each of the right basis vectors that span the common HO GSVD subspace is a generalized singular vector of all pairwise GSVD factorizations of the matrices and with equal corresponding generalized singular values for all and . Since the GSVD can be computed in a stable way, the common HO GSVD subspace can also be computed in a stable way by computing all pairwise GSVD factorizations of the matrices and . That is, the common HO GSVD subspace exists also for matrices that are not all of full column rank. This also means that the common HO GSVD subspace can be formulated as a solution to an optimization problem, in analogy with existing variational formulations of the GSVD [33].

It would be ideal if our procedure reduced to the stable computation of the matrix GSVD when . To achieve this ideal, we would need to find a procedure that allows a computation of the HO GSVD, not just the common HO GSVD subspace, for matrices that are not all of full column rank. A formulation of the HO GSVD, not just the common HO GSVD subspace, as a solution to an optimization problem may lead to a stable numerical algorithm for computing the HO GSVD. Such a formulation may also lead to a higher-order general Gauss-Markov linear statistical model [34]–[36].

It was shown that the GSVD provides a mathematical framework for sequence-independent comparative modeling of DNA microarray data from two organisms, where the mathematical variables and operations represent experimental or biological reality [7], [8]. The variables, subspaces of significant patterns that are common to both or exclusive to either one of the datasets, correlate with cellular programs that are conserved in both or unique to either one of the organisms, respectively. The operation of reconstruction in the subspaces common to both datasets outlines the biological similarity in the regulation of the cellular programs that are conserved across the species. Reconstruction in the common and exclusive subspaces of either dataset outlines the differential regulation of the conserved relative to the unique programs in the corresponding organism. Recent experimental results [9] verify a computationally predicted genome-wide mode of regulation [10], [11], and demonstrate that GSVD modeling of DNA microarray data can be used to correctly predict previously unknown cellular mechanisms.

Here we showed, comparing global cell-cycle mRNA expression from the three disparate organisms *S. pombe*, *S. cerevisiae* and human, that the HO GSVD provides a sequence-independent comparative framework for two or more genomic datasets, where the variables and operations represent biological reality. The approximately common HO GSVD subspace represents the cell-cycle mRNA expression oscillations, which are similar among the datasets. Simultaneous reconstruction in the common subspace removes the experimental artifacts, which are dissimilar, from the datasets. In the simultaneous sequence-independent classification of the genes of the three organisms in this common subspace, genes of highly conserved sequences but significantly different cell-cycle peak times are correctly classified.

Additional possible applications of our HO GSVD in biotechnology include comparison of multiple genomic datasets, each corresponding to (*i*) the same experiment repeated multiple times using different experimental protocols, to separate the biological signal that is similar in all datasets from the dissimilar experimental artifacts; (*ii*) one of multiple types of genomic information, such as DNA copy number, DNA methylation and mRNA expression, collected from the same set of samples, e.g., tumor samples, to elucidate the molecular composition of the overall biological signal in these samples; (*iii*) one of multiple chromosomes of the same organism, to illustrate the relation, if any, between these chromosomes in terms of their, e.g., mRNA expression in a given set of samples; and (*iv*) one of multiple interacting organisms, e.g., in an ecosystem, to illuminate the exchange of biological information in these interactions.

## Supporting Information

#### Appendix S1

A PDF format file, readable by Adobe Acrobat Reader.

(PDF)

^{(2.7M, pdf)}

#### Mathematica Notebook S1

**Higher-order generalized singular value decomposition (HO GSVD) of global mRNA expression datasets from three different organisms.** A Mathematica 5.2 code file, executable by Mathematica 5.2 and readable by Mathematica Player, freely available at http://www.wolfram.com/products/player/.

(NB)

^{(16M, nb)}

#### Mathematica Notebook S2

**HO GSVD of global mRNA expression datasets from three different organisms.** A PDF format file, readable by Adobe Acrobat Reader.

(PDF)

^{(4.6M, pdf)}

#### Dataset S1

*S. pombe*** global mRNA expression.** A tab-delimited text format file, readable by both Mathematica and Microsoft Excel, reproducing the relative mRNA expression levels of =3167 *S. pombe* gene clones at =17 time points during about two cell-cycle periods from Rustici *et al.* [15] with the cell-cycle classifications of Rustici *et al.* or Oliva *et al.* [16].

(TXT)

^{(571K, txt)}

#### Dataset S2

*S. cerevisiae*** global mRNA expression.** A tab-delimited text format file, readable by both Mathematica and Microsoft Excel, reproducing the relative mRNA expression levels of =4772 *S. cerevisiae* open reading frames (ORFs), or genes, at =17 time points during about two cell-cycle periods, including cell-cycle classifications, from Spellman *et al.* [17].

(TXT)

^{(548K, txt)}

#### Dataset S3

**Human global mRNA expression.** A tab-delimited text format file, readable by both Mathematica and Microsoft Excel, reproducing the relative mRNA expression levels of =13,068 human genes at =17 time points during about two cell-cycle periods, including cell-cycle classifications, from Whitfield *et al.* [18].

(TXT)

^{(1.5M, txt)}

## Acknowledgments

We thank G. H. Golub for introducing us to matrix and tensor computations, and the American Institute of Mathematics in Palo Alto and Stanford University for hosting the 2004 Workshop on Tensor Decompositions and the 2006 Workshop on Algorithms for Modern Massive Data Sets, respectively, where some of this work was done. We also thank C. H. Lee for technical assistance, R. A. Horn for helpful discussions of matrix analysis and careful reading of the manuscript, and L. De Lathauwer and A. Goffeau for helpful comments.

## Footnotes

**Competing Interests: **The authors have declared that no competing interests exist.

**Funding: **This research was supported by Office of Naval Research Grant N00014-02-1-0076 (to MAS), National Science Foundation Grant DMS-1016284 (to CFVL), as well as the Utah Science Technology and Research (USTAR) Initiative, National Human Genome Research Institute R01 Grant HG-004302 and National Science Foundation CAREER Award DMS-0847173 (to OA). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

## References

^{rum1}cdk inhibitor. EMBO J. 1996;15:839–849. [PMC free article] [PubMed]

^{cdc2}kinase promotes both S-phase and mitosis in the absence of G1 cyclins. EMBO J. 1996;15:850–860. [PMC free article] [PubMed]

**Public Library of Science**

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- Generalized singular value decomposition for comparative analysis of genome-scale expression data sets of two different organisms.[Proc Natl Acad Sci U S A. 2003]
*Alter O, Brown PO, Botstein D.**Proc Natl Acad Sci U S A. 2003 Mar 18; 100(6):3351-6. Epub 2003 Mar 11.* - DISCO-SCA and properly applied GSVD as swinging methods to find common and distinctive processes.[PLoS One. 2012]
*Van Deun K, Van Mechelen I, Thorrez L, Schouteden M, De Moor B, van der Werf MJ, De Lathauwer L, Smilde AK, Kiers HA.**PLoS One. 2012; 7(5):e37840. Epub 2012 May 31.* - Incremental linear discriminant analysis for face recognition.[IEEE Trans Syst Man Cybern B Cybern. 2008]
*Zhao H, Yuen PC.**IEEE Trans Syst Man Cybern B Cybern. 2008 Feb; 38(1):210-21.* - A tensor higher-order singular value decomposition for integrative analysis of DNA microarray data from different studies.[Proc Natl Acad Sci U S A. 2007]
*Omberg L, Golub GH, Alter O.**Proc Natl Acad Sci U S A. 2007 Nov 20; 104(47):18371-6. Epub 2007 Nov 14.* - RNA maturation in mitochondria of S. cerevisiae and S. pombe.[Gene. 2005]
*Schäfer B.**Gene. 2005 Jul 18; 354:80-5.*

- SYSTEMS VIROLOGY: HOST-DIRECTED APPROACHES TO VIRAL PATHOGENESIS AND DRUG TARGETING[Nature reviews. Microbiology. 2013]
*Law GL, Korth MJ, Benecke AG, Katze MG.**Nature reviews. Microbiology. 2013 Jul; 11(7)455-466* - Global State Measures of the Dentate Gyrus Gene Expression System Predict Antidepressant-Sensitive Behaviors[PLoS ONE. ]
*Samuels BA, Leonardo ED, Dranovsky A, Williams A, Wong E, Nesbitt AM, McCurdy RD, Hen R, Alter M.**PLoS ONE. 9(1)e85136* - Multi-tissue Analysis of Co-expression Networks by Higher-Order Generalized Singular Value Decomposition Identifies Functionally Coherent Transcriptional Modules[PLoS Genetics. ]
*Xiao X, Moreno-Moral A, Rotival M, Bottolo L, Petretto E.**PLoS Genetics. 10(1)e1004006* - Inferring Gene Regulatory Networks by Singular Value Decomposition and Gravitation Field Algorithm[PLoS ONE. ]
*Zheng M, Wu JN, Huang YX, Liu GX, Zhou Y, Zhou CG.**PLoS ONE. 7(12)e51141* - DISCO-SCA and Properly Applied GSVD as Swinging Methods to Find Common and Distinctive Processes[PLoS ONE. ]
*Van Deun K, Van Mechelen I, Thorrez L, Schouteden M, De Moor B, van der Werf MJ, De Lathauwer L, Smilde AK, Kiers HA.**PLoS ONE. 7(5)e37840*

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