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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Science. Author manuscript; available in PMC Dec 22, 2011.
Published in final edited form as:
PMCID: PMC3244756
NIHMSID: NIHMS344392

RNAi-Mediated Targeting of Heterochromatin by the RITS Complex

Abstract

RNA interference (RNAi) is a widespread silencing mechanism that acts at both the posttranscriptional and transcriptional levels. Here, we describe the purification of an RNAi effector complex termed RITS (RNA-induced initiation of transcriptional gene silencing) that is required for heterochromatin assembly in fission yeast. The RITS complex contains Ago1 (the fission yeast Argonaute homolog), Chp1 (a heterochromatin-associated chromodomain protein), and Tas3 (a novel protein). In addition, the complex contains small RNAs that require the Dicer ribonuclease for their production. These small RNAs are homologous to centromeric repeats and are required for the localization of RITS to heterochromatic domains. The results suggest a mechanism for the role of the RNAi machinery and small RNAs in targeting of heterochromatin complexes and epigenetic gene silencing at specific chromosomal loci.

The fission yeast Schizosaccharomyces pombe contains large stretches of heterochromatin that are associated with telomeres, repetitive DNA elements surrounding centromeres, and with the silent mating-type loci (1). Assembly of heterochromatin at these loci involves an orchestrated array of chromatin modifications that lead to the recruitment of two chromo-domain histone-binding proteins Swi6, a homolog of the Drosophila and mammalian HP1 proteins, and Chp1 (2, 3). The RNAi pathway has also been implicated in regulation at the DNA and chromatin level in Arabidopsis (46), Drosophila (7), and Tetrahymena (8), and in heterochromatin assembly in S. pombe (9, 10).

RNAi silencing is triggered by double-stranded RNA (dsRNA), which is cleaved by the ribonuclease III (RNase III)–like enzyme Dicer to generate small RNA molecules of ~22 nucleotides (nt) (1113). These small interfering RNAs (siRNAs), load onto an effector complex called RISC (RNA-induced silencing complex) that contains an Argonaute/PIWI family protein and targets cognate mRNAs for inactivation (1215).

Factors involved in the RNAi pathway in other organisms are required for heterochromatin formation in S. pombe. Deletion of any of these factors, such as Dicer (dcr1+), Argonaute (ago1+), and RNA-dependent RNA polymerase (rdp1+), disrupts heterochromatin assembly (9, 10). In support of a role for RNAi in heterochromatin assembly, both DNA strands of the S. pombe centromeric repeats are transcribed (9), and siRNAs have been identified that match the S. pombe centromeric repeats (16). Moreover, recent experiments suggest that artificial generation of dsRNA from a hairpin construct can silence homologous sequences by heterochromatin formation in an RNAi-dependent manner (17). Here, we address the key question of how small RNAs generated by the RNAi machinery initiate heterochromatin assembly in fission yeast.

To identify factors important for RNAi-mediated targeting of heterochromatin complexes, we reasoned that such factor(s) would act in early steps in heterochromatin assembly and would be required for the establishment of heterochromatin-specific histone modification patterns. The Chp1 protein binds to centromeric repeats and is required for methylation of histone H3-K9 and for localization of Swi6 (3, 18). Moreover, the phenotypes displayed by chp1Δ strains are identical to RNAi mutants. To test whether Chp1 provides a physical and functional link between RNAi and heterochromatin assembly, we used a tandem affinity purification procedure (TAP) and a TAP tag to identify factors that interact with Chp1 (Fig. 1). Several protein species of about 65, 90, 100, and 120 kD were specifically purified from the Chp1-TAP strain (Fig. 1A). Mass spectrometry of excised gel bands, as well as protein mixtures, identified the 120- and 100-kD bands as Chp1, the 90-kD band as Ago1, and the 65-kD band as SPBC83.03c, a previously uncharacterized protein (Fig. 1, A and C; table S1; figs. S1 and S2), which we named Tas3 (targeting complex subunit 3). The ratio of the 120- and 100-kD bands varies from experiment to experiment, which suggests that the 100-kD protein is a degradation product of Chp1.

Fig. 1
Purification of Chp1-TAP and identification of associated proteins. Extracts from a Chp1-TAP strain and an untagged control strain were purified by the TAP procedure and applied to a 4 to 12% polyacrylamide gel, which was stained with colloidal Coomassie ...

To verify that Chp1, Ago1, and Tas3 are associated together in a complex, we constructed an S. pombe strain that produced a fully functional Tas3-TAP protein (Fig. 2, A and B). Affinity purification followed by mass spectrometry sequencing identified Ago1 and Chp1 as Tas3-associated proteins (Fig. 2C, table S1). N- or C-terminally tagged Ago1 proteins were not functional in centromeric silencing and were not used for purification experiments. However, identical purification profiles of Chp1-TAP and Tas3-TAP suggests that Chp1, Ago1, and Tas3 are associated together in a complex, which we have named RITS.

Fig. 2
Purification of the RITS complex by using a Tas3-TAP strain and the requirement of tas3+ in silencing and methylation of H3-K9 and Swi6 localization. Western blot showing that (A) the Tas3-TAP and Chp1-TAP proteins are expressed to similar levels and ...

Chp1, as well as Ago1 and other components of the RNAi pathway, have previously been shown to be required for the assembly of heterochromatin and silencing of reporter genes inserted within heterochromatic domains (9, 10, 19, 20). A tas3 deletion strain carrying the ura4+ reporter gene inserted at innermost (imr) or outermost (otr) centromeric repeats of chromosome 1 (imr1R::ura4+ and otr1R::ura4+, respectively) displayed a loss of silencing of both reporter genes (Fig. 2D) to an extent similar to that of the deletion of sir2, chp1, or ago1 (Fig. 2D) (9, 10, 19, 21). Further, chromatin immunoprecipitation (ChIP) showed that Tas3 was required for H3-K9 methylation and Swi6 localization of a ura4+ reporter gene inserted at each of the above loci (Fig. 2E).

As is the case for RNAi mutants (10), deletion of tas3+ had little or no effect on silencing or localization of H3-K9 methylation and Swi6 to the ura4+ reporter gene inserted at the mat locus (Kint2::ura4+) (Fig. 2, D and E). The similarity in phenotypes displayed by tas3Δ, chp1Δ, and RNAi mutants underscores the importance of Tas3 interaction with Chp1 and the role of the RITS complex in RNAi-mediated hetero-chromatin assembly.

Members of the Argonaute family of proteins constitute the core subunit of RISC, which is associated with small RNA molecules that target it to specific mRNAs (12, 13). To determine whether the RITS complex is associated with small RNA molecules, we subjected Chp1-TAP or control purifications to phenol-chloroform extraction and precipitated the aqueous phase of the extraction containing any nucleic acid. The precipitated material was then labeled with [5′-32P]pCp and T4 RNA ligase (22). As shown in Fig. 3A, Chp1-TAP is specifically associated with small RNA molecules ranging in size from ~22 to 25 nt. In contrast, the predominant RNA species prepared from a whole-cell extract (total RNA) are 70 to 100 nt in size, most likely representing transfer RNA (tRNA) and 5S RNA (Fig. 3A). RNA species, mainly in the size range of abundant tRNAs, as well as a small amount of an RNA species of ~25 nt, were present in both the untagged control and Chp1-TAP purification and represent nonspecific background binding (Fig. 3A, lanes 2 to 4). Similar results were obtained when the RITS complex was purified from a strain producing Tas3-TAP (Fig. 3B).

Fig. 3
Dicer-dependent association of RITS with siRNAs. (A) Small RNAs of ~22 to 25 nt copurify with Chp1-TAP. RNAs isolated from untagged control (−) and Chp1-TAP (+) strains were 3′ end-labeled with [5′-32P]pCp and separated on 15% ...

siRNAs are produced by the ribonuclease Dicer (12, 13). We purified the RITS complex from a strain that carried a deletion of dcr1+, the only S. pombe gene that codes for Dicer. Deletion of dcr1+ resulted in a loss of small RNA species that specifically copurify with Chp1-TAP but had no effect on the presence of nonspecific RNA species, which were also present in the untagged control purification (Fig. 3C). These results indicated that the small RNA species specifically associated with RITS are siRNAs that are produced in a Dcr1-dependent manner.

Sequencing of small RNAs from S. pombe has identified a series of small RNA species that are complementary to the centromeric repeat sequences (16). These small RNAs have been termed heterochromatic siRNAs and are clustered at two regions within the centromeric repeats, the dh repeats and a region immediately downstream of the dg repeats. Centromeric siRNAs have been proposed to function in sequence-specific targeting of homologous DNA regions (i.e., centromeric repeats) for heterochromatin assembly. To determine whether siRNAs associated with RITS originate from centromeric repeats, we first analyzed RITS-associated RNAs on a Northern blot probed with a mixture of oligonucleotides derived from the centromeric repeats. These oligonucleotides were specifically designed to hybridize to siRNAs previously identified by Reinhart and Bartel (16). The 32P-labeled oligonucleotide probes specifically hybridized to RNA species of ~22 to 25 nt in size present in the Chp1-TAP purification but not with nonspecific RNAs present in the untagged control purification (Fig. 3D).

As a second test for the identities of the siRNAs associated with RITS, we labeled RITS-associated siRNAs with [5′-32P]pCp, then gel purified and used them to probe a Southern blot containing equal amounts of DNA fragments (ranging in size from 300 to 700 base pairs) corresponding to the dg and dh centromeric repeats, the region downstream of dg repeats to which siRNAs map (designated dg-D), retrotransposon long terminal repeats (LTRs) that have been shown to mediate RNAi-dependent gene silencing (17), and DNA fragments corresponding to actin and molecular size markers. The labeled siRNAs specifically hybridized to dg, dh, and dg-D centromeric sequences (Fig. 3E). No hybridization was detected to LTR, actin, or DNA size markers (Fig. 3E). Our inability to detect hybridization of RITS-associated siRNAs with LTR sequences may be due to a relatively lower abundance of LTR siRNAs compared with siRNAs that originate from the centromeric repeats. Together, these experiments show that RITS is associated with siRNAs that originate from processing of centromeric dsRNA transcripts.

We next used S. pombe strains that produced either Tas3-TAP or Chp1-Flag to determine the in vivo chromatin localization of the RITS complex and the requirement for the RNAi pathway in its localization. It has previously been shown that Chp1 localizes to the centromeric repeat regions and together with the Clr4 methyltransferase is required for H3-K9 methylation and Swi6 localization (3). ChIP experiments showed that Tas3-TAP is similarly localized to a ura4+ reporter gene inserted within in the otr centromeric repeat region (otr1::ura4+) and centromeric repeat sequences but not to the control mini-ura4 (ura4DS/E) gene at the endogenous euchromatic location (Fig. 4). Tas3-TAP, like Chp1 (18), is also localized to the imr centromeric repeats (Fig. 4D). Furthermore, deletion of ago1+, dcr1+, or rdp1+ abolished the association of Chp1-Flag and Tas3-TAP with otr1::ura4+, as well as with centromeric repeat sequences (Fig. 4, A and B). These results indicated that the RNAi pathway is required for association of the Chp1 and Tas3 subunits of RITS with heterochromatic DNA regions. Our purification of the RITS complex from dcr1Δ cells showed that the protein subunits of the complex remained associated together in the absence of siRNAs (fig. S4). The purification results, together with the ChIP analysis, indicate that the “empty” RITS complex is inactive and can only associate with its chromosomal target after it is programmed by siRNAs.

Fig. 4
The RNAi pathway is required for localization of RITS to heterochromatin. (A) ChIP experiments showing that Tas3-TAP is localized to centromeric heterochromatin in an RNAi-dependent manner. Tas3-TAP is associated with ura4+ inserted at the otr centromeric ...

We further tested whether Tas3 was required for the localization of Chp1-Flag to each of the above regions. Deletion of tas3+ abolished the association of Chp1-Flag with otr1::ura4+, as well as with native cen sequences (Fig. 4C). These results support the biochemical identification of Tas3 as an integral subunit of RITS and indicate that it plays an essential role in localizing the complex to heterochromatin.

Our analysis suggests a remarkably direct role for the RNAi machinery in heterochromatin assembly. By analogy to RISC complexes, which use small RNAs as guides to target specific mRNAs for degradation or translational repression, we propose that RITS uses siRNAs to recognize and to bind to specific chromosome regions so as to initiate heterochromatic gene silencing (Fig. 5). Four lines of evidence support this view. First, RITS contains Ago1, the S. pombe homolog of the Argonaute family of proteins, which form the common subunit of RISC complexes purified from different organisms and are thought to be directly responsible for target recognition (12). Second, RITS is associated with siRNAs that require Dcr1 for their formation and originate from heterochromatin repeat regions. Thus, this complex contains the expected specificity determinants, i.e., siRNAs, which in other systems have been shown to direct target recognition (14, 15, 23, 24). Third, at least two subunits of the RITS complex, Chp1 and Tas3, are specifically associated with the expected heterochromatic DNA regions, which suggests that the complex localizes directly to its target DNA. Fourth, in addition to Ago1, RITS contains a chromodomain protein, Chp1, which is localized throughout heterochromatic DNA regions (18) (Fig. 4) and requires the methyltransferase Clr4 and histone H3-K9 methylation for localization to chromatin (3, 18). Thus, RITS contains both a subunit (Ago1) that binds to siRNAs and can function in RNA or DNA targeting by sequence-specific pairing interaction and a subunit (Chp1) that associates with specifically modified histones and may be involved in further stabilizing its association with chromatin (Fig. 5).

Fig. 5
A model for siRNA-dependent initiation of heterochromatin assembly by RITS. The RITS complex is programmed by Dcr1-produced siR-NAs to target specific chromosome regions by sequence-specific interactions involving either siRNA-DNA or siRNA-nascent transcript ...

Mechanisms analogous to the RITS-mediated targeting of heterochromatin complexes are likely to be conserved in other systems. For example, in Tetrahymena, genomewide DNA elimination during macronucleus development requires an Argonaute family protein, Twi1, and a chromodomain protein, Pdd1, both of which are also required for H3-K9 methylation and accumulation of small RNAs corresponding to target sequences (8, 25). Similarly, in Drosophila repeat-induced transcriptional gene silencing requires an Argonaute family protein, Piwi, and a chromodomain protein, Polycomb (7). Our results support the hypothesis that Argonaute proteins form the core subunit of a number of different effector complexes that use sequence-specific recognition to target either RNA or DNA.

Supplementary Material

Verdel-Suppl-2004

Acknowledgments

We thank M. Ohi, K. Gould, C. Hoffman, and D. Wolf for gifts of strains and plasmids; members of the Moazed, Grewal, and Reed laboratories for support and encouragement; R Ohi and El C. Ibrahim for advice; El C. Ibrahim and M. Wahi for comments on the manuscript; and C. Centrella for technical help. A.V. was supported by a postdoctoral fellowship from INSERM and is now a fellow of the Human Frontier Science Programme. This work was supported by grants from the NIH (S.I.S.G. and D.M.) and a Carolyn and Peter S. Lynch Award in Cell Biology and Pathology (D.M.). D.M. is a scholar of the Leukemia and Lymphoma Society.

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