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Nucleic Acids Res. Oct 10, 1980; 8(19): 4321–4325.
PMCID: PMC324241

Rapid isolation of high molecular weight plant DNA.

Abstract

A method is presented for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length) which is free of contaminants which interfere with complete digestion by restriction endonucleases. The procedure yields total cellular DNA (i.e. nuclear, chloroplast, and mitochondrial DNA). The technique is ideal for the rapid isolation of small amounts of DNA from many different species and is also useful for large scale isolations.

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Selected References

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  • JONES AS. The isolation of bacterial nucleic acids using cetyltrimethylammonium bromide (cetavlon). Biochim Biophys Acta. 1953 Apr;10(4):607–612. [PubMed]
  • RALPH RK, BELLAMY AR. ISOLATION AND PURIFICATION OF UNDEGRADED RIBONUCLEIC ACIDS. Biochim Biophys Acta. 1964 May 18;87:9–16. [PubMed]
  • Murray MG, Palmer JD, Cuellar RE, Thompson WF. Deoxyribonucleic acid sequence organization in the mung bean genome. Biochemistry. 1979 Nov 13;18(23):5259–5266. [PubMed]

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