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Nucleic Acids Res. May 24, 1980; 8(10): 2119–2132.
PMCID: PMC324067

The transcription termination site at the end of the early region of bacteriophage T7 DNA.


The nucleotide sequence through the transcription termination site for Escherichia coli RNA polymerase at the end of the early region of T7 DNA has been determined. RNA chains terminate at adjacent residues in the DNA sequence: about 2/3 of the chains end in C and 1/3 in G. A potential stem and loop structure, containing a stem of eight uninterrupted base pairs and a four-base loop, is centered 17-18 bases ahead of the chain termini. Transcription by E. coli RNA polymerase terminates efficiently at this site in vivo and in vitro, but transcription by T7 RNA polymerase is essentially unaffected. There are no primary cleavage sites for RNase III near the transcription termination site. The site of termination lies within HpaI fragment Q of T7 DNA, whose entire 446-nucleotide long sequence was determined. Cleavage sites for other restriction endonucleases are located conveniently for manipulating the DNA sequence around the termination site. The coding sequence for the last 82 amino acids of the T7 DNA ligase protein was identified, as was the beginning of a coding sequence for a possible late T7 protein from beyond the termination site.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Studier FW. Bacteriophage T7. Science. 1972 Apr 28;176(4033):367–376. [PubMed]
  • Dunn JJ, Studier FW. T7 early RNAs are generated by site-specific cleavages. Proc Natl Acad Sci U S A. 1973 May;70(5):1559–1563. [PMC free article] [PubMed]
  • Minkley EG, Pribnow D. Transcription of the early region of bacteriophage T7: selective initiation with dinucleotides. J Mol Biol. 1973 Jun 25;77(2):255–277. [PubMed]
  • Kiefer M, Neff N, Chamberlin MJ. Transcriptional termination at the end of the early region of bacteriophages T3 and T7 is not affected by polarity suppressors. J Virol. 1977 May;22(2):548–552. [PMC free article] [PubMed]
  • Studier FW, Rosenberg AH, Simon MN, Dunn JJ. Genetic and physical mapping in the early region of bacteriophage T7 DNA. J Mol Biol. 1979 Dec 25;135(4):917–937. [PubMed]
  • Dunn JJ. RNase III cleavage of single-stranded RNA. Effect of ionic strength on the fideltiy of cleavage. J Biol Chem. 1976 Jun 25;251(12):3807–3814. [PubMed]
  • Lacks S, Greenberg B. Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation. J Mol Biol. 1977 Jul;114(1):153–168. [PubMed]
  • McDonell MW, Simon MN, Studier FW. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977 Feb 15;110(1):119–146. [PubMed]
  • Dunn JJ, Buzash-Pollert E, Studier FW. Mutations of bacteriophage T7 that affect initiation of synthesis of the gene 0.3 protein. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2741–2745. [PMC free article] [PubMed]
  • Studier FW. Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973 Sep 15;79(2):237–248. [PubMed]
  • England TE, Uhlenbeck OC. 3'-terminal labelling of RNA with T4 RNA ligase. Nature. 1978 Oct 12;275(5680):560–561. [PubMed]
  • Maxam AM, Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. [PMC free article] [PubMed]
  • Jay E, Bambara R, Padmanabhan R, Wu R. DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping. Nucleic Acids Res. 1974 Mar;1(3):331–353. [PMC free article] [PubMed]
  • Donis-Keller H, Maxam AM, Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. [PMC free article] [PubMed]
  • Oakley JL, Coleman JE. Structure of a promoter for T7 RNA polymerase. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4266–4270. [PMC free article] [PubMed]
  • Oakley JL, Strothkamp RE, Sarris AH, Coleman JE. T7 RNA polymerase: promoter structure and polymerase binding. Biochemistry. 1979 Feb 6;18(3):528–537. [PubMed]
  • Rosa MD. Four T7 RNA polymerase promoters contain an identical 23 bp sequence. Cell. 1979 Apr;16(4):815–825. [PubMed]
  • Panayotatos N, Wells RD. Recognition and initiation site for four late promoters of phage T7 is a 22-base pair DNA sequence. Nature. 1979 Jul 5;280(5717):35–39. [PubMed]
  • Boothroyd JC, Hayward RS. New genes and promoters suggested by the DNA sequence near the end of the coliphage T7 early operon. Nucleic Acids Res. 1979 Dec 11;7(7):1931–1943. [PMC free article] [PubMed]
  • Dunn JJ, Studier FW. Processing transcription, and translation of bacteriophage T7 messenger RNAs. Brookhaven Symp Biol. 1975 Jul;(26):267–276. [PubMed]
  • Shine J, Dalgarno L. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342–1346. [PMC free article] [PubMed]
  • Pachl CA, Yound ET. The size and messenger RNA activity of bacteriophage T7 late transcripts synthesized in vivo. J Mol Biol. 1978 Jun 15;122(1):69–101. [PubMed]
  • Peters GG, Hayward RS. Transcriptional termination in vitro: the 3'-terminal sequence of coliphage T7 "early" RNA. Biochem Biophys Res Commun. 1974 Nov 27;61(2):809–816. [PubMed]
  • Peters GG, Hayward RS. Dinucleotide sequences in the regions of T7 DNA coding for termination of early transcription. Eur J Biochem. 1974 Oct 1;48(1):199–208. [PubMed]

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