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Nucleic Acids Res. Apr 11, 1980; 8(7): 1499–1504.
PMCID: PMC324012

DNA methylation and the frequency of CpG in animal DNA.


An analysis of nearest neighbour dinucleotide frequencies and the level of DNA methylation in animals strongly supports the suggestion that 5-methylcytosine (5mC) tends to mutate abnormally frequently to T. This tendency is the likely cause of the CpG deficiency in heavily methylated genomes.

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Selected References

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  • JOSSE J, KAISER AD, KORNBERG A. Enzymatic synthesis of deoxyribonucleic acid. VIII. Frequencies of nearest neighbor base sequences in deoxyribonucleic acid. J Biol Chem. 1961 Mar;236:864–875. [PubMed]
  • SWARTZ MN, TRAUTNER TA, KORNBERG A. Enzymatic synthesis of deoxyribonucleic acid. XI. Further studies on nearest neighbor base sequences in deoxyribonucleic acids. J Biol Chem. 1962 Jun;237:1961–1967. [PubMed]
  • Bird AP, Taggart MH. Variable patterns of total DNA and rDNA methylation in animals. Nucleic Acids Res. 1980 Apr 11;8(7):1485–1497. [PMC free article] [PubMed]
  • Rae PM, Steele RE. Absence of cytosine methylation at C-C-G-G and G-C-G-C sites in the rDNA coding regions and intervening sequences of Drosophila and the rDNA of other insects. Nucleic Acids Res. 1979 Jul 11;6(9):2987–2995. [PMC free article] [PubMed]
  • Waalwijk C, Flavell RA. MspI, an isoschizomer of hpaII which cleaves both unmethylated and methylated hpaII sites. Nucleic Acids Res. 1978 Sep;5(9):3231–3236. [PMC free article] [PubMed]
  • Singer J, Roberts-Ems J, Riggs AD. Methylation of mouse liver DNA studied by means of the restriction enzymes msp I and hpa II. Science. 1979 Mar 9;203(4384):1019–1021. [PubMed]
  • Bird AP, Taggart MH, Smith BA. Methylated and unmethylated DNA compartments in the sea urchin genome. Cell. 1979 Aug;17(4):889–901. [PubMed]
  • WYATT GR. The purine and pyrimidine composition of deoxypentose nucleic acids. Biochem J. 1951 May;48(5):584–590. [PMC free article] [PubMed]
  • Vanyushin BF, Tkacheva SG, Belozersky AN. Rare bases in animal DNA. Nature. 1970 Mar 7;225(5236):948–949. [PubMed]
  • CHARGAFF E, LIPSHITZ R, GREEN C. Composition of the desoxypentose nucleic acids of four genera of sea-urchin. J Biol Chem. 1952 Mar;195(1):155–160. [PubMed]
  • Russell GJ, Walker PM, Elton RA, Subak-Sharpe JH. Doublet frequency analysis of fractionated vertebrate nuclear DNA. J Mol Biol. 1976 Nov;108(1):1–23. [PubMed]
  • Russell GJ, Subak-Sharpe JH. Similarity of the general designs of protochordates and invertebrates. Nature. 1977 Apr 7;266(5602):533–536. [PubMed]
  • Coulondre C, Miller JH, Farabaugh PJ, Gilbert W. Molecular basis of base substitution hotspots in Escherichia coli. Nature. 1978 Aug 24;274(5673):775–780. [PubMed]
  • Subak-Sharpe H, Bürk RR, Crawford LV, Morrison JM, Hay J, Keir HM. An approach to evolutionary relationships of mammalian DNA viruses through analysis of the pattern of nearest neighbor base sequences. Cold Spring Harb Symp Quant Biol. 1966;31:737–748. [PubMed]
  • Elton RA. Doublet frequencies in sequenced nucleic acids. J Mol Evol. 1975 Mar 24;4(4):323–346. [PubMed]
  • Brownlee GG, Cartwright E, McShane T, Williamson R. The nucleotide sequence of somatic 5 S RNA from Xenopus laevis. FEBS Lett. 1972 Sep 1;25(1):8–12. [PubMed]
  • Wegnez M, Monier R, Denis H. Sequence heterogeneity of 5 S RNA in Xenopus laevis. FEBS Lett. 1972 Sep 1;25(1):13–20. [PubMed]
  • Ford PJ, Southern EM. Different sequences for 5S RNA in kidney cells and ovaries of Xenopus laevis. Nat New Biol. 1973 Jan 3;241(105):7–12. [PubMed]
  • Miller JR, Cartwright EM, Brownlee GG, Fedoroff NV, Brown DD. The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. II. The GC-rich region. Cell. 1978 Apr;13(4):717–725. [PubMed]
  • Mandel JL, Chambon P. DNA methylation: organ specific variations in the methylation pattern within and around ovalbumin and other chicken genes. Nucleic Acids Res. 1979 Dec 20;7(8):2081–2103. [PMC free article] [PubMed]
  • Scarano E, Iaccarino M, Grippo P, Parisi E. The heterogeneity of thymine methyl group origin in DNA pyrimidine isostichs of developing sea urchin embryos. Proc Natl Acad Sci U S A. 1967 May;57(5):1394–1400. [PMC free article] [PubMed]

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