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Proc Natl Acad Sci U S A. Jul 1986; 83(14): 5291–5295.
PMCID: PMC323937

Production of a monoclonal antibody specific for seminomas and dysgerminomas.

Abstract

A monoclonal antibody (M2A, IgG2a) was produced against a cultured human ovarian epithelial adenocarcinoma cell line, HEY. Monoclonal antibody M2A reacted with a glycoprotein of molecular weight 40,000 on the surface of HEY cells. The affinity constant of the monoclonal antibody M2A for HEY cells was 10(9) M-1, and the number of binding sites on HEY cells was 2 X 10(4) per cell. The monoclonal antibody produced positive immunoperoxidase staining of fetal (but not adult) testis and of seminomas and dysgerminomas but did not stain various normal adult tissues or other gonadal or extragonadal tumors. Monoclonal antibody M2A may be useful for confirming a histological diagnosis of seminoma and dysgerminoma.

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  • Mostofi FK. Proceedings: Testicular tumors. Epidemiologic, etiologic, and pathologic features. Cancer. 1973 Nov;32(5):1186–1201. [PubMed]
  • Bush SE, Martinez A, Bagshaw MA. Primary mediastinal seminoma. Cancer. 1981 Oct 15;48(8):1877–1882. [PubMed]
  • Houston RR, Winter CC, Smith JP, Brenner MA. Primary retroperitoneal seminoma without gonadal involvement: a report of 3 cases. J Urol. 1971 Dec;106(6):841–844. [PubMed]
  • Kasper CS, Schneider NR, Childers JH, Wilson JD. Suprasellar germinoma. Unresolved problems in diagnosis, pathogenesis, and management. Am J Med. 1983 Oct;75(4):705–711. [PubMed]
  • Battifora H, Sheibani K, Tubbs RR, Kopinski MI, Sun TT. Antikeratin antibodies in tumor diagnosis. Distinction between seminoma and embryonal carcinoma. Cancer. 1984 Sep 1;54(5):843–848. [PubMed]
  • Miettinen M, Virtanen I, Talerman A. Intermediate filament proteins in human testis and testicular germ-cell tumors. Am J Pathol. 1985 Sep;120(3):402–410. [PMC free article] [PubMed]
  • Cohen C, Shulman G, Budgeon LR. Immunohistochemical ferritin in testicular seminoma. Cancer. 1984 Nov 15;54(10):2190–2194. [PubMed]
  • Mahony J, Bose A, Cowdrey D, Nusair T, Lei M, Harris J, Marks A, Baumal R. A monoclonal antiidiotypic antibody to MOPC 315 IgA inhibits the growth of MOPC 315 myeloma cells in vitro. J Immunol. 1981 Jan;126(1):113–117. [PubMed]
  • Buick RN, Pullano R, Trent JM. Comparative properties of five human ovarian adenocarcinoma cell lines. Cancer Res. 1985 Aug;45(8):3668–3676. [PubMed]
  • Shulman M, Wilde CD, Köhler G. A better cell line for making hybridomas secreting specific antibodies. Nature. 1978 Nov 16;276(5685):269–270. [PubMed]
  • Marks A, Law J, Mahony JB, Baumal R. The structural conservation of S100 protein during evolution: analysis by reactivity with a monoclonal antibody. J Neurochem. 1983 Jul;41(1):107–112. [PubMed]
  • Frankel ME, Gerhard W. The rapid determination of binding constants for antiviral antibodies by a radioimmunoassay. An analysis of the interaction between hybridoma proteins and influenza virus. Mol Immunol. 1979 Feb;16(2):101–106. [PubMed]
  • Bolivar I. Immunological crossreactivity of Paramecium surface antigens: conformational differences account for cell surface specificity. Proc Natl Acad Sci U S A. 1983 Feb;80(4):1003–1007. [PMC free article] [PubMed]
  • Burk KH, Oefinger PE, Dreesman GR. Detection of non-A, non-B hepatitis antigen by immunocytochemical staining. Proc Natl Acad Sci U S A. 1984 May;81(10):3195–3199. [PMC free article] [PubMed]
  • Coffin CM, Ewing S, Dehner LP. Frequency of intratubular germ cell neoplasia with invasive testicular germ cell tumors. Histologic and immunocytochemical features. Arch Pathol Lab Med. 1985 Jun;109(6):555–559. [PubMed]

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