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Proc Natl Acad Sci U S A. May 1986; 83(10): 3086–3090.
PMCID: PMC323457

Hepatocellular influx of [14C]oleate reflects membrane transport rather than intracellular metabolism or binding.

Abstract

When [14C]oleate bound to bovine serum albumin was incubated at 37 degrees C for 7 min with isolated rat hepatocytes in the absence of glucose, the cumulative oleate uptake curve had two components: a rapid, linear segment over the first 30 sec, followed by a slower, curvilinear component. At 173 microM [14C]oleate/albumin (1:1, mol/mol), the initial component had a slope (Vo) of 118 +/- 18 pmol per min per 5 X 10(4) hepatocytes (mean +/- SD). During this initial 30 sec, virtually no oleate was oxidized, and less than 11% was esterified. By 5 min, 79% was esterified; oxidation never exceeded 4%. Addition of 2 mM glucose significantly increased oleate esterification and thereby available oleate binding sites on cytosolic fatty acid binding protein but had no influence on Vo. Pretreatment with trypsin reduced Vo by 49 +/- 15%. These data indicate that the initial component of the oleate uptake curve reflects predominantly influx, whereas the subsequent component reflects a balance between influx, efflux, and intracellular metabolism. Influx is independent of intracellular binding, oxidation, and esterification and may reflect a membrane-associated carrier-mediated process.

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  • Kuhl WE, Spector AA. Uptake of long-chain fatty acid methyl esters by mammalian cells. J Lipid Res. 1970 Sep;11(5):458–465. [PubMed]
  • Mahadevan S, Sauer F. Effect of trypsin, phospholipases, and membrane-impermeable reagents on the uptake of palmitic acid by isolated rat liver cells. Arch Biochem Biophys. 1974 Sep;164(1):185–193. [PubMed]
  • Stremmel W, Kochwa S, Berk PD. Studies of oleate binding to rat liver plasma membranes. Biochem Biophys Res Commun. 1983 Apr 15;112(1):88–95. [PubMed]
  • Stremmel W, Strohmeyer G, Borchard F, Kochwa S, Berk PD. Isolation and partial characterization of a fatty acid binding protein in rat liver plasma membranes. Proc Natl Acad Sci U S A. 1985 Jan;82(1):4–8. [PMC free article] [PubMed]
  • Abumrad NA, Perkins RC, Park JH, Park CR. Mechanism of long chain fatty acid permeation in the isolated adipocyte. J Biol Chem. 1981 Sep 10;256(17):9183–9191. [PubMed]
  • Abumrad NA, Park JH, Park CR. Permeation of long-chain fatty acid into adipocytes. Kinetics, specificity, and evidence for involvement of a membrane protein. J Biol Chem. 1984 Jul 25;259(14):8945–8953. [PubMed]
  • Paris S, Samuel D, Romey G, Ailhaud G. Uptake of fatty acids by cultured cardiac cells from chick embryo: evidence for a facilitation process without energy dependence. Biochimie. 1979;61(3):361–367. [PubMed]
  • Samuel D, Paris S, Ailhaud G. Uptake and metabolism of fatty acids and analogues by cultured cardiac cells from chick embryo. Eur J Biochem. 1976 May 1;64(2):583–595. [PubMed]
  • DeGrella RF, Light RJ. Uptake and metabolism of fatty acids by dispersed adult rat heart myocytes. I. Kinetics of homologous fatty acids. J Biol Chem. 1980 Oct 25;255(20):9731–9738. [PubMed]
  • DeGrella RF, Light RJ. Uptake and metabolism of fatty acids by dispersed adult rat heart myocytes. II. Inhibition by albumin and fatty acid homologues, and the effect of temperature and metabolic reagents. J Biol Chem. 1980 Oct 25;255(20):9739–9745. [PubMed]
  • Kushlan MC, Gollan JL, Ma WL, Ockner RK. Sex differences in hepatic uptake of long chain fatty acids in single-pass perfused rat liver. J Lipid Res. 1981 Mar;22(3):431–436. [PubMed]
  • Christiansen RZ. Regulation of palmitate metabolism by carnitine and glucagon in hepatocytes isolated from fasted and carbohydrate refed rats. Biochim Biophys Acta. 1977 Aug 24;488(2):249–262. [PubMed]
  • Berry MN, Friend DS. High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study. J Cell Biol. 1969 Dec;43(3):506–520. [PMC free article] [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Reichen J, Blitzer BL, Berk PD. Binding of unconjugated and conjugated sulfobromophthalein to rat liver plasma membrane fractions in vitro. Biochim Biophys Acta. 1981 Jan 8;640(1):298–312. [PubMed]
  • Belfrage P, Vaughan M. Simple liquid-liquid partition system for isolation of labeled oleic acid from mixtures with glycerides. J Lipid Res. 1969 May;10(3):341–344. [PubMed]
  • Macey RI, Farmer RE. Inhibition of water and solute permeability in human red cells. Biochim Biophys Acta. 1970 Jul 7;211(1):104–106. [PubMed]
  • Levi AJ, Gatmaitan Z, Arias IM. Two hepatic cytoplasmic protein fractions, Y and Z, and their possible role in the hepatic uptake of bilirubin, sulfobromophthalein, and other anions. J Clin Invest. 1969 Nov;48(11):2156–2167. [PMC free article] [PubMed]
  • Ockner RK, Manning JA. Fatty acid-binding protein in small intestine. Identification, isolation, and evidence for its role in cellular fatty acid transport. J Clin Invest. 1974 Aug;54(2):326–338. [PMC free article] [PubMed]
  • Ockner RK, Manning JA, Poppenhausen RB, Ho WK. A binding protein for fatty acids in cytosol of intestinal mucosa, liver, myocardium, and other tissues. Science. 1972 Jul 7;177(4043):56–58. [PubMed]

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