• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Feb 1986; 83(3): 696–700.
PMCID: PMC322931

Multiplicity of satellite DNA sequences in Drosophila melanogaster.

Abstract

Three Drosophila melanogaster satellite DNAs (1.672, 1.686, and 1.705 g/ml in CsCl), each containing a simple sequence repeated in tandem, were cloned in pBR322 as small fragments about 500 base pairs long. This precaution minimized deletions, since inserts of the same size as the fragments used for cloning were recovered in a stable form. A homogeneous tandem array of one sequence type usually extended the length of the insert. Eleven distinct repeat sequences were discovered, but only one sequence was predominant in each satellite preparation. The remaining classes were minor in amount. The repeat unit lengths were restricted to 5, 7, or 10 base pairs, with sequences closely related. Each sequence conforms to the expression (RRN)m(RN)n, where R is A or G. The multiplicity of simple repeated sequences revealed despite the small sample size suggests that numerous repeat sequences reside in heterochromatin and that particular rules apply to the structure of the repeating sequence.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Peacock WJ, Lohe AR, Gerlach WL, Dunsmuir P, Dennis ES, Appels R. Fine structure and evolution of DNA in heterochromatin. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):1121–1135. [PubMed]
  • Peacock WJ, Brutlag D, Goldring E, Appels R, Hinton CW, Lindsley DL. The organization of highly repeated DNA sequences in Drosophila melanogaster chromosomes. Cold Spring Harb Symp Quant Biol. 1974;38:405–416. [PubMed]
  • Endow SA, Polan ML, Gall JG. Satellite DNA sequences of Drosophila melanogaster. J Mol Biol. 1975 Aug 25;96(4):665–692. [PubMed]
  • Sederoff R, Lowenstein L. Polypyrimidine segments in Drosophila melanogaster DNA: II. Chromosome location and nucleotide sequence. Cell. 1975 Jun;5(2):183–194. [PubMed]
  • Carlson M, Brutlag D. Cloning and characterization of a complex satellite DNA from Drosophila melanogaster. Cell. 1977 Jun;11(2):371–381. [PubMed]
  • Hsieh T, Brutlag D. Sequence and sequence variation within the 1.688 g/cm3 satellite DNA of Drosophila melanogaster. J Mol Biol. 1979 Dec 5;135(2):465–481. [PubMed]
  • Carlson M, Brutlag D. Different regions of a complex statellite DNA vary in size and sequence of the repeating unit. J Mol Biol. 1979 Dec 5;135(2):483–500. [PubMed]
  • Brutlag D, Fry K, Nelson T, Hung P. Synthesis of hybrid bacterial plasmids containing highly repeated satellite DNA. Cell. 1977 Mar;10(3):509–519. [PubMed]
  • Brutlag D, Carlson M, Fry K, Hsieh TS. DNA sequence organization in Drosophila heterochromatin. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):1137–1146. [PubMed]
  • Brutlag D, Appels R, Dennis ES, Peacock WJ. Highly repeated DNA in Drosophila melanogaster. J Mol Biol. 1977 May 5;112(1):31–47. [PubMed]
  • Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. [PubMed]
  • Smith DR, Calvo JM. Nucleotide sequence of the E coli gene coding for dihydrofolate reductase. Nucleic Acids Res. 1980 May 24;8(10):2255–2274. [PMC free article] [PubMed]
  • Kafatos FC, Jones CW, Efstratiadis A. Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. Nucleic Acids Res. 1979 Nov 24;7(6):1541–1552. [PMC free article] [PubMed]
  • BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. [PMC free article] [PubMed]
  • Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. [PMC free article] [PubMed]
  • Sadler JR, Tecklenburg M, Betz JL. Plasmids containing many tandem copies of a synthetic lactose operator. Gene. 1980 Feb;8(3):279–300. [PubMed]
  • Steffensen DM, Appels R, Peacock WJ. The distribution of two highly repeated DNA sequences within Drosophila melanogaster chromosomes. Chromosoma. 1981;82(4):525–541. [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Birnboim HC. Polypyrimidine segments in Drosophila melanogaster DNA: I. Detection of a cryptic satellite containing polypyrimidine/polypurine DNA. Cell. 1975 Jun;5(2):173–181. [PubMed]
  • Gall JG, Atherton DD. Satellite DNA sequences in Drosophila virilis. J Mol Biol. 1974 Jan 5;85(4):633–664. [PubMed]
  • Mullins JI, Blumenfeld M. Satellite Ic: a possible link between the satellite DNAs of D. virilis and D. melanogaster. Cell. 1979 Jul;17(3):615–621. [PubMed]
  • Singh L, Phillips C, Jones KW. The conserved nucleotide sequences of Bkm, which define Sxr in the mouse, are transcribed. Cell. 1984 Jan;36(1):111–120. [PubMed]
  • Urieli-Shoval S, Gruenbaum Y, Sedat J, Razin A. The absence of detectable methylated bases in Drosophila melanogaster DNA. FEBS Lett. 1982 Sep 6;146(1):148–152. [PubMed]
  • Achwal CW, Ganguly P, Chandra HS. Estimation of the amount of 5-methylcytosine in Drosophila melanogaster DNA by amplified ELISA and photoacoustic spectroscopy. EMBO J. 1984 Feb;3(2):263–266. [PMC free article] [PubMed]
  • Collins J, Volckaert G, Nevers P. Precise and nearly-precise excision of the symmetrical inverted repeats of Tn5; common features of recA-independent deletion events in Escherichia coli. Gene. 1982 Jul-Aug;19(1):139–146. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...