• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of narLink to Publisher's site
Nucleic Acids Res. Oct 25, 1985; 13(20): 7473–7481.
PMCID: PMC322056

Sequence of the T4 recombination gene, uvsX, and its comparison with that of the recA gene of Escherichia coli.

Abstract

We have determined the nucleotide sequence of the uvsX gene of bacteriophage T4 which is involved in DNA recombination and damage repair, and whose product catalyzes in vitro reactions related to recombination process in analogous manners to E. coli recA gene product. The coding region consisted of 1170 nucleotides directing the synthesis of a polypeptide of 390 amino acids in length with a calculated molecular weight of 43,760. Amino acid composition, the sequence of seven NH2-terminal amino acids and molecular weight of the protein deduced from the nucleotide sequence were consistent with the data from the analysis of the purified uvsX protein. The nucleotide sequence and the deduced amino acid sequence were compared with those of the recA gene. Although a significant homology was not found in the nucleotide sequences, the amino acid sequences included 23% of identical and 15% of conservatively substituted residues.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (961K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Hamlett NV, Berger H. Mutations altering genetic recombination and repair of DNA in bacteriophage T4. Virology. 1975 Feb;63(2):539–567. [PubMed]
  • Cunningham RP, Berger H. Mutations affecting genetic recombination in bacteriophage T4D. I. Pathway analysis. Virology. 1977 Jul 1;80(1):67–82. [PubMed]
  • Yonesaki T, Ryo Y, Minagawa T, Takahashi H. Purification and some of the functions of the products of bacteriophage T4 recombination genes, uvsX and uvsY. Eur J Biochem. 1985 Apr 1;148(1):127–134. [PubMed]
  • CLARK AJ, MARGULIES AD. ISOLATION AND CHARACTERIZATION OF RECOMBINATION-DEFICIENT MUTANTS OF ESCHERICHIA COLI K12. Proc Natl Acad Sci U S A. 1965 Feb;53:451–459. [PMC free article] [PubMed]
  • Ogawa T, Wabiko H, Tsurimoto T, Horii T, Masukata H, Ogawa H. Characteristics of purified recA protein and the regulation of its synthesis in vivo. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):909–915. [PubMed]
  • Weinstock GM, McEntee K, Lehman IR. ATP-dependent renaturation of DNA catalyzed by the recA protein of Escherichia coli. Proc Natl Acad Sci U S A. 1979 Jan;76(1):126–130. [PMC free article] [PubMed]
  • Shibata T, DasGupta C, Cunningham RP, Radding CM. Purified Escherichia coli recA protein catalyzes homologous pairing of superhelical DNA and single-stranded fragments. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1638–1642. [PMC free article] [PubMed]
  • McEntee K, Weinstock GM, Lehman IR. Initiation of general recombination catalyzed in vitro by the recA protein of Escherichia coli. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2615–2619. [PMC free article] [PubMed]
  • Cox MM, Lehman IR. recA protein of Escherichia coli promotes branch migration, a kinetically distinct phase of DNA strand exchange. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3433–3437. [PMC free article] [PubMed]
  • Horii T, Ogawa T, Nakatani T, Hase T, Matsubara H, Ogawa H. Regulation of SOS functions: purification of E. coli LexA protein and determination of its specific site cleaved by the RecA protein. Cell. 1981 Dec;27(3 Pt 2):515–522. [PubMed]
  • Griffith J, Formosa T. The uvsX protein of bacteriophage T4 arranges single-stranded and double-stranded DNA into similar helical nucleoprotein filaments. J Biol Chem. 1985 Apr 10;260(7):4484–4491. [PubMed]
  • Takahashi H, Saito H. Cloning of uvsW and uvsY genes of bacteriophage T4. Virology. 1982 Jul 15;120(1):122–129. [PubMed]
  • Bolivar F. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules. Gene. 1978 Oct;4(2):121–136. [PubMed]
  • Shigesada K, Tsurushita N, Matsumoto Y, Imai M. Overproduction of transcription termination factor Rho in Escherichia coli. Gene. 1984 Jul-Aug;29(1-2):199–209. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Norrander J, Kempe T, Messing J. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene. 1983 Dec;26(1):101–106. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Childs JD. Isolation and genetic properties of a bacteriophage T4 uvsX mutant. Mutat Res. 1980 Jun;71(1):1–14. [PubMed]
  • Mattson T, Van Houwe G, Bolle A, Selzer G, Epstein R. Genetic identification of cloned fragments of bacteriophage T4 DNA and complementation by some clones containing early T4 genes. Mol Gen Genet. 1977 Sep 9;154(3):319–326. [PubMed]
  • Coleman JE, Oakley JL. Physical chemical studies of the structure and function of DNA binding (helix-destabilizing) proteins. CRC Crit Rev Biochem. 1980 Jan;7(3):247–289. [PubMed]
  • Walker JM, Gooderham K, Hastings JR, Mayes E, Johns EW. The primary structures of non-histone chromosomal proteins HMG 1 and 2. FEBS Lett. 1980 Dec 29;122(2):264–270. [PubMed]
  • Williams KR, LoPresti MB, Setoguchi M, Konigsberg WH. Amino acid sequence of the T4 DNA helix-destabilizing protein. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4614–4617. [PMC free article] [PubMed]
  • Dunn JJ, Studier FW. Nucleotide sequence from the genetic left end of bacteriophage T7 DNA to the beginning of gene 4. J Mol Biol. 1981 Jun 5;148(4):303–330. [PubMed]
  • Walker JE, Saraste M, Runswick MJ, Gay NJ. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1982;1(8):945–951. [PMC free article] [PubMed]
  • Horii T, Ogawa T, Ogawa H. Organization of the recA gene of Escherichia coli. Proc Natl Acad Sci U S A. 1980 Jan;77(1):313–317. [PMC free article] [PubMed]
  • Sancar A, Stachelek C, Konigsberg W, Rupp WD. Sequences of the recA gene and protein. Proc Natl Acad Sci U S A. 1980 May;77(5):2611–2615. [PMC free article] [PubMed]
  • Doolittle RF. Similar amino acid sequences: chance or common ancestry? Science. 1981 Oct 9;214(4517):149–159. [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...