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PLoS One. 2011; 6(11): e27754.
Published online Nov 16, 2011. doi:  10.1371/journal.pone.0027754
PMCID: PMC3218036

Gene Decay in Shigella as an Incipient Stage of Host-Adaptation

David Liberles, Editor

Abstract

Background

Many facultative bacterial pathogens have undergone extensive gene decay processes, possibly due to lack of selection pressure during evolutionary conversion from free-living to intracellular lifestyle. Shigella, the causative agents of human shigellosis, have arisen from different E. coli-like ancestors independently by convergent paths. As these bacteria all have lost large numbers of genes by mutation or deletion, they can be used as ideal models for systematically studying the process of gene function loss in different bacteria living under similar selection pressures.

Methodologies/Principal Findings

We compared the sequenced Shigella genomes and re-defined decayed genes (pseudogenes plus deleted genes) in these bacteria. Altogether, 85 genes are commonly decayed in the five analyzed Shigella strains and 1456 genes are decayed in at least one Shigella strain. Genes coding for carbon utilization, cell motility, transporter or membrane proteins are prone to be inactivated. Decayed genes tend to concentrate in certain operons rather than distribute averagely across the whole genome. Genes in the decayed operon accumulated more non-synonymous mutations than the rest genes and meanwhile have lower expression levels.

Conclusions

Different Shigella lineages underwent convergent gene decay processes, and inactivation of one gene would lead to a lesser selection pressure for the other genes in the same operon. The pool of superfluous genes for Shigella may contain at least two thousand genes and the gene decay processes may still continue in Shigella until a minimum genome harboring only essential genes is reached.

Introduction

Bacterial genomes are usually tightly packed with protein-coding genes and other functional elements. From the evolutionary point of view, a bacterial genome stays at a general balance in terms of genetic material gains and losses. On the one hand, it receives exogenous DNA in order to acquire extra fitness traits for better adapting to the external environment, and on the other hand the genome itself is undergoing a shrinking process, mainly in the forms of pseudogenization and deletion. Supporting evidence includes the finding that the majority of pseudogenes are newly-born genes and the derived young pseudogenes may be expelled out of genome soon [1], [2]. Direct deletion can also occur to throw out DNA fragments of various sizes [3].

When bacteria evolve from free-living to an intracellular lifestyle, a balance between DNA flowing in and out to maintain a certain genomic size may vanish. In this process, some bacterial pathogens may accumulate a large number of pseudogenes, such as Mycobacterium leprae [4], which has half of its genes inactivated, and some others may even get rid of all non-essential genes to keep a minimum genome, such as Mycoplasma genitalium [5]. Perhaps the most important cause leading to genome shrinking is natural selection: genes that no longer make significant contributions to bacterial fitness may subject to decay (including pseudogene formation and complete deletion). Genetic drift is another important factor [6], [7], [8]. Especially for some highly pathogenic bacteria, which typically require only small inocula to elicit infection in their host, the effective population size is dramatically reduced. Such extreme population bottleneck greatly diminishes the efficacy of selection, under which even beneficial genes are prone to be eliminated. In addition, loss of certain genes will sometimes contribute to fitness, so the bacteria would tend not to express these genes. Such “adaptive loss” has been reported in Shigella. For example, expression of cadA in Shigella would inhibit the enterotoxin activity, therefore reducing the virulence. So this gene is usually inactivated in Shigella [9].

Shigella are human-restricted pathogens that are highly contagious. According to the traditional taxonomy, the Shigella genus consists of four species: S. dysenteriae, S. flexneri, S. boydii and S. sonnei [10]. However, modern genotyping methods, especially comparative genomic studies, have revealed that the four Shigella species are phylogenetically interwoven with the E. coli lineages, suggesting that distinct ancestral E. coli lineages developed into similar pathogens through convergent evolutionary processes by acquisition of common pathogenic traits [11], [12], [13]. Extensive gene decay has been documented in representative strains of all Shigella species [14], [15], [16], [17] and this process occurs at a much faster speed in Shigella than in typical E. coli [18], possibly reflecting a key series of evolutionary events in the conversion of Shigella from commensals into intracellular pathogens.

In this study we used Shigella as the model to investigate key issues about bacterial gene decay, including how convergent gene decay occurs in the process when bacteria of different origins adapt to the same biological niche, and how many superfluous genes will eventually be decayed. Different from previous studies, this study is characteristic of two aspects. First, we defined convergent pseudogenization as different inactivation mutations affecting the same protein. As a result, the majority of pseudogenes shared by different Shigella species belong to the convergent instead of ancestral pseudogene category. Second, we classified genes into decayed genes, intact genes in the decayed operons and the rest genes. In addition to demonstrating the existence of genome shrinking process as well as the lesser selection pressure combined with low expression of decayed genes, this operon-based analysis also delimits the minimal number of potentially superfluous genes.

Results and Discussion

Identification of decayed genes and genomic reduction of Shigella

We used the genome of Escherichia coli K12 MG1655 (abbr. K12) as the reference sequence to identify gene loss in Shigella. The K12 genome has two special advantages for use as the reference: its vast sequence similarity with the Shigella strains and its largely experiment-based annotation [19]. Especially important is the fact that the gene boundaries of many CDSs of K12 have been verified by experiments, which would greatly facilitate pseudogene identification. The Shigella strains analyzed included S. boydii Sb227 (SBO), S. dysenteriae Sd197 (SDY), S. flexneri 2a 301 (SF2a), S. flexneri 5 8401 (SF5), and S. sonnei Ss046 (SSON). We also included E. coli O157:H7 EDL933 (O157), E. coli CFT073 (CFT073) and E. coli IAI1 (IAI1) in this study for comparison with Shigella, because the three strains as well as K12 belong to different E. coli phylogroups and therefore can represent a broad range of E. coli sublineages [20]. To reveal the genetic relationships among these strains, we built a phylogentic tree based on all conserved genes among them (Figure 1).

Figure 1
Phylogenetic tree of E. coli/Shigella strains and the number of decayed genes.

As expected, all Shigella strains had greatly more genes decayed (including both pseudogene formation and deletion) than E. coli (Figure 1; Table S1). Of particular interest, the numbers of decayed genes differ considerably among the Shigella species. For instance, SDY had nearly twice as many genes decayed as SSON, suggesting that SDY was the earliest Shigella lineage to become adapted to the human host. A total of 85 genes decayed in all five Shigella strains analyzed (Table S2), providing candidates for further scrutiny about possible positive selection of their absence. The previous reported gene cadA [9] was also in this list.

The Pearson correlation coefficient between the numbers of pseudogenes and deleted genes is close to 0.9, suggesting that pseudogenization and deletion occurred simultaneously. Some other findings also support this hypothesis. The two S. flexneri strains have diverged very recently. Despite the short divergence time, SF2a and SF5 each have 17 and 14 strain-specific deleted genes, respectively; meanwhile, they each have 14 and 10 strain-specific truncated pseudogenes (Table S1). Thus genomic reduction may not necessarily have started with pseudogenes formation first followed by deletion; direct deletion also seems to be a common event in the genomic evolution of bacteria.

Convergent gene decay among Shigella species

Shared pseudogenes among the Shigella strains account for nearly one quarter of all pseudogenes in each strain (Table S1). We further classified the shared pseudogenes into three groups based on the inactivation causes. The first group is ancestral pseudogenes, which have the same inactivation mutations among the compared bacteria and therefore have probably been inherited from their last common ancestor. Only a minority of shared pseudogenes can be assigned to the ancestral situation in all comparisons. The only exception was with SF2a and SF5, which share most of their ancestral pseudogenes. They belong to the same S. flexneri species and therefore should have diverged not long ago.

The second group is convergent pseudogenes, which have inactivation mutations affecting different sites of the same gene among the bacteria and therefore have probably been formed after the divergence of the bacteria. Most of the shared pseudogenes belong to the convergence situation (Table S1), consistent with the hypothesis that Shigella lineages have developed from multiple origins (which is also indicated by the phylogenetic trees; see Figure 1). During their convergent paths to adapt to the same biological niche, a common set of genes would become superfluous, so the convergent pseudogenes should reflect the common superfluous functions.

The third group is recombinant pseudogenes, which have the same inactivation mutation among the compared strains but the mutation conflicts with the phylogenetic relationships. For example, SF2a and SBO shared the same pseudogene but the same gene remained intact in SF5 (a member of the same species as SF2a) and in other strains. Then the most plausible explanation is that such a pseudogene must have resulted from recombination that copied the mutation from SBO to SF2a. A total of 27 putative recombinant pseudogenes were identified, and 14 of them are found between S. boydii and S. flexneri (Table S3). The recombination rate in Shigella was in fact quite low: 0.013 for the five Shigella strains as compared to 0.056 for the four E. coli strains (see Text S1). In the real world, it should be quite unlikely that two different Shigella species might infect the same person at the same time. Consequently, recombination should not be considered as a major route of pseudogene formation as that between Salmonella typhi and Salmonella paratyphi A, two human-restricted Salmonella species [21].

Relatedness among SDY and O157 is quite intriguing. The two strains were clustered at the same branch of the phylogenetic tree as shown in Figure 1. It was once hypothesized that the ancestor of the two bacterial lineages had headed on the evolutionary paths toward becoming Shigella, but O157 lost the Shigella virulence plasmid and went back to a lower rate of gene loss [18]. In our analysis, the two strains share 30 pseudogenes, only one of which belongs to the ancestral category (Table S4), providing evidence against the above hypothesis. Here we prefer an alternative hypothesis: they were living in a common environment for a certain time period after their divergence, and the environment facilitated formation of the convergent pseudogenes.

The pool of superfluous genes for Shigella

As many as 1456 genes have decayed through pseudogenization or deletion in one or more of the five Shigella strains analyzed (Table S2). The extensive gene decay, involving mostly the convergent evolutionary paths, suggests that these genes are superfluous (at least non-essential) functionally in the Shigella species. An interesting question is how large the pool of superfluous genes might be or whether there would be an end of the gene decaying process.

We categorized the 1456 genes by function using the COG and GenProtEC classification systems. Analyses by the two classification systems led to common conclusions: genes coding for carbon utilization, cell motility, transporter or membrane proteins are prone to be inactivated, whereas those responsible for maintenance of basic cellular metabolism and structure tend to resist inactivation (Chi square test, p value < 0.001; Table 1). This suggests that the reduction process will at least have a threshold, namely ending up with a minimum genome that contains only essential genes like Mycoplasma genitalium [5].

Table 1
Differential functional assignment between decayed genes and intact genes.

In bacteria, the basic functioning genetic unit is the operon, which contains a cluster of genes under the control of the same set of regulatory signals. Therefore, the inactivation of one gene may result in functional relaxation on the other genes within the same operon, starting the gene decay process. We searched for the operons in which the decayed genes are located with the premise that all genes in these “decayed operons” would be eventually eliminated. Meanwhile, we calculated the percentage of decayed genes over total genes within the operon for each strain. This index is highly correlated with the number of decayed genes (Figure 2). In other words, decayed genes are indeed more concentrated within certain operons rather than being scattered averagely in the whole genome. The 1456 decayed genes were located in 832 operons, which contain a total of 2048 genes (Table S2). We believe that this is the minimum size of the superfluous gene pool.

Figure 2
Correlation between number of decayed genes and percentage of decayed genes in operon.

Functional relaxation and low expression of pseudogenes

We hypothesized that pseudogenization would accelerate the accumulation of non-synonymous mutations in the pseudogenes as well as intact genes in the decayed operons (operons containing decayed genes). So these genes were expected to exhibit a higher Ka/Ks value in comparison with the rest genes. To look into this, we classified genes into three categories: Pseudogenes (Group P), the Intact genes in the pseudo-operons (Group I) and the Rest functional genes in the genome (Group R). We found that in SDY, the Ka/Ks values of group P and group I are significantly higher than group R, whereas between group P and group I no significant difference was detected (Kruskal-Wallis test with Dunn's post test, p<0.01; Figure 3). This result demonstrated the accuracy of operon-based classification and, in the meantime, showed the synchronous evolving speed for the genes in the same operon: the whole operon would decay immediately once one of its genes becomes inactivated, followed by relaxed selection pressure on the rest genes in the same operon. In other Shigella strains, no significant difference was found among the three groups. Given that SDY had undergone the most extensive gene decay and therefore might be the earliest Shigella lineage to become adapted to the human host, we presume that the other Shigella species have not had enough time yet for accumulating non-synonymous mutations like SDY.

Figure 3
Comparison of Ka/Ks between different gene groups in SDY.

Will pseudogenized genes be expressed and, if yes, how much? The relationship between pseudogenization and expression levels may involve two questions. The first is whether genes of low expression are more prone to become pseudogenized, and the second is whether genes would decrease the expression levels after pseudogenization. For the first question, we compared the Codon Adaptation Index (CAI) between the genes in decayed operon and the rest genes, since this index has long been used to predict expression levels [22], [23]. The result showed that the former did have lower CAI values than the latter (Unpaired t test, p value < 0.001; Figure 4). It is likely that genes of low expression are only occasionally expressed when the bacteria encounter certain environmental stimuli. Once living in a relatively stable environment, loss of these genes would not cause obvious adverse effects and therefore gene decay may ensue.

Figure 4
Comparison of CAI between genes in decayed operon and the rest genes in the genome.

For the second question, we resorted to microarray-based transcription data. A total of eight microarray samples that measured the expression profile of Shigella were deposited in NCBI GEO database. The original purpose of these microarray experiments covers comparison between conditions under different temperatures, or comparison between in-vivo and in-vitro conditions, or comparison between before and after curation of virulence plasmid, etc. Nearly all these samples exhibited lower expression of genes in decayed operons than the rest genes (Kruskal-Wallis test with Dunn's post test, p value < 0.01). This phenomenon can be explained as the following: the bacteria try to avoid extra investment of resource and energy on the decayed genes. Additionally, malfunctioning proteins encoded by pseudogenes may be detrimental to the bacteria by interacting with other pathways, e.g., by losing specificity or competing for substrates [1]. However, the pseudogenes and the intact genes within the decayed operons have similar expression, demonstrating the read-through transcription of both intact genes and pseudogenes together within the same operon.

Materials and Methods

Construction of orthologs and Identification of decayed genes

The coding sequences (CDSs) from E. coli K12 MG1655 were mapped to query genomes for ortholog identification by using NCBI Basic Local Alignment Search Tool (BLAST), with the criteria being identity > 75% and e-value < 1e-10. Matches that do not conform to chromosomal colinearity were removed manually.

A total of 2500 orthologs were common to all analyzed genomes and were used for constructing a genome-scale phylogenetic tree. For this, individual orthologous sequences were aligned by using MAFFT [24] and were then concatenated to obtain a “chromosomal” alignment; the phylogenetic tree was constructed with the program PHYML under GTR+gamma+I model [25].

Pseudogenes were identified by using the Psi-Fi perl script [26]. Briefly, genes that contain frameshifts, nonsense mutations, truncations or insertion/deletions (indels) that altered >20% of the amino acid sequence in comparison with the reference sequence were treated as pseudogenes. The exact site in which the pseudogenization event occurs was identified by using GeneWise software [27], which helps to align the pseudogene with its functional ortholog from the reference genome. Genes present in one Shigella strain but absent from the other Shigella strain were considered as a deletion event of the latter strain. If one gene is present in all the four E. coli strains but absent from the entire five Shigella strains, this gene is considered to be commonly decayed in all Shigella strains.

Operon definition

The organization of operons in E. coli K12 MG1655 was taken from the database MicrobesOnline Operon Predictions [28] and RegulonDB [29]. Additionally, adjacent genes that have been proven to be involved in the same biological pathway (e.g., thrL, thrA, thrB and thrC) were considered to be of the same operon. A home-made Perl script was written for calculating the number of pseudogenes in their located operon.

Functional assignment of Shigella decayed genes

COG (Cluster of Orthologous Groups of proteins) information was retrieved from NCBI COG database [30]. GenProtEC information was retrieved from E. coli genome and proteome databases [31]. We computed the number of genes for each function category and then used Chi-square test to detect which functional category differed significantly between decayed genes and functional genes.

Ka/Ks analysis

The synonymous (Ks) and non-synonymous (Ka) substitution rates of the Shigella strains in comparison with the reference genome of E. coli K12 MG1655 were calculated by using codeml program in PAML package [32]. Genes were divided into three groups according the operon information. We did not compare Ka/Ks between groups directly in order to minimize the bias brought by the inherent differences between genes themselves; instead we compared ΔKa/Ks. CFT073 was used as the control, because this strain stays outside of all Shigella strains phylogenetically. For each orthologous gene, ΔKa/Ks  = Ka/KsShigella – Ka/KsCFT073, in which Ka/KsShigella is Ka/Ks between a certain Shigella strain and K12, and Ka/KsCFT073 is between E. coli CFT073 and K12.

Expression potential of pseudogenes

The microarray-based transcription information of S. flexneri was retrieved from NCBI GEO database (eight sample records: GSM314300GSM314307). The "codon adaptation index" (CAI) values for E. coli K12 genes was calculated with codonW program (http://codonw.sourceforge.net/).

Supporting Information

Table S1

Detailed list of decayed genes in the five Shigella strains.

(XLS)

Table S2

Detailed list of (A) genes commonly decayed in the five Shigella strains, and (B) genes decayed in at least one Shigella strain, and (C) genes in all decayed operon in Shigella.

(XLS)

Table S3

Recombination of pseudogenes in Shigella.

(XLS)

Table S4

Pseudogenes shared by SDY and O157.

(XLS)

Text S1

Comparison of recombination rate between E. coli and Shigella.

(DOC)

Acknowledgments

We are grateful to Emmanuelle Lerat and Howard Ochman for kindly providing the Psi-Fi perl script.

Footnotes

Competing Interests: The authors have declared that no competing interests exist.

Funding: This work was supported by a grant from Harbin Medical University, grants of National Natural Science Foundation of China (NSFC30870098, 30970119, 81030029) and Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP, 20092307110001) to SLL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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