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Nucleic Acids Res. 1984 Jan 11; 12(1 Pt 2): 789–800.
PMCID: PMC321093

Escherichia coli promoter sequences predict in vitro RNA polymerase selectivity.


We describe a simple algorithm for computing a homology score for Escherichia coli promoters based on DNA sequence alone. The homology score was related to 31 values, measured in vitro, of RNA polymerase selectivity, which we define as the product KBk2, the apparent second order rate constant for open complex formation. We found that promoter strength could be predicted to within a factor of +/-4.1 in KBk2 over a range of 10(4) in the same parameter. The quantitative evaluation was linked to an automated (Apple II) procedure for searching and evaluating possible promoters in DNA sequence files.

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Selected References

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  • Hawley DK, McClure WR. In vitro comparison of initiation properties of bacteriophage lambda wild-type PR and x3 mutant promoters. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6381–6385. [PMC free article] [PubMed]
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