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Nucleic Acids Res. Sep 11, 1984; 12(17): 6663–6671.
PMCID: PMC320107

Codon usage can affect efficiency of translation of genes in Escherichia coli.


By inserting synthetic oligonucleotides into a highly expressed gene in E. coli it has been shown that unfavourable codon usage can reduce the maximum translation rate of a protein. However, in the case of the codon used (AGG), a significant effect on translation was only seen at very high transcription rates from a gene containing multiple copies of the unfavourable codon.

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Selected References

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