Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
PMC full text:
Structure. Author manuscript; available in PMC 2012 Oct 12.
Published in final edited form as:
Structure. 2011 Oct 12; 19(10): 1443–1455.
doi:  10.1016/j.str.2011.07.012

Figure 7

An external file that holds a picture, illustration, etc.
Object name is nihms-316788-f0007.jpg

The activity of the MUN-CD module in promoting the syntaxin-1/Munc18-1 to SNARE complex transition is severely impaired. A. Left panel: superposition of expansions of 1H-13C HMQC spectra of 15 μM 2H-I-13CH3-syntaxin-1 bound to Munc18-1 (black contours) or incorporated into SNARE complex formed with synaptobrevin and SNAP-25 SNARE motifs [2H-LV-13CH3-synaptobrevin(29-93), 2H-SNAP-25(11-82) and 2H-SNAP-25(141-203)] (red contours). The expansion shows the cross-peak corresponding to I203 (Ma et al., 2011). Middle and right panels: 1H-13C HMQC spectra of 15 μM 2H-I-13CH3-syntaxin-1 bound to Munc18-1, taken after 39 or 119 min after adding 15 μM 2H-Val,Leu-13CH3-synaptobrevin(29-93), 25 μM 2H-SNAP-25(11-82) and 25 μM 2H-SNAP-25(141-203), in the presence of 30 μM MUN-CD. The elongated shape of the cross-peaks in these panels arises because these 1H-13C HMQC spectra were acquired for only 10 minutes each and thus have low resolution in the 13C dimension. B. Fraction of SNARE complex formed as function of time in the same experiments as panel A, monitored through the intensity of the I203 cross-peak of the SNARE complex in consecutive 1H-13C HMQC spectra. Up 119 min, the spectra were acquired every 10 minutes, and afterwards every 2 hours (the cross-peak intensities were normalized accordingly). The curve shows the fit to an exponential rise to a maximum.

Images in this article

  • Figure 1
  • Figure 2
  • Figure 3
  • Figure 4
  • Figure 5
  • Figure 6
  • Figure 7
Click on the image to see a larger version.