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Proc Natl Acad Sci U S A. 1981 May; 78(5): 2856–2860.
PMCID: PMC319457

Histone genes are clustered but not tandemly repeated in the chicken genome.


The recombinant chicken DNA library was screened for histone genes by using pSp17, a recombinant sea urchin DNA probe containing the H2a and H3 genes of Strongylocentrotus purpuratus. Three of the isolated lambda recombinants have been analyzed by restriction enzyme mapping and Southern blotting; one histone H3 gene-encoding recombinant was further analyzed by DNA sequence determination. These studies reveal that the chicken histone genes are not tandemly reiterated, but that, at the least, several histone genes are physically closely linked in a nonrepetitive arrangement within the chicken genome. The evolutionary implications of this arrangement versus that seen in Drosophila and sea urchins is discussed.

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Selected References

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  • Kedes LH, Cohn RH, Lowry JC, Chang AC, Cohen SN. The organization of sea urchin histone genes. Cell. 1975 Nov;6(3):359–369. [PubMed]
  • Weinberg ES, Overton GC, Shutt RH, Reeder RH. Histone gene arrangement in the sea urchin, Strongylocentrotus purpuratus. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4815–4819. [PMC free article] [PubMed]
  • Arceci RJ, Senger DR, Gross PR. The programmed switch in lysine-rich histone synthesis at gastrulation. Cell. 1976 Sep;9(1):171–178. [PubMed]
  • Lifton RP, Goldberg ML, Karp RW, Hogness DS. The organization of the histone genes in Drosophila melanogaster: functional and evolutionary implications. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):1047–1051. [PubMed]
  • Kedes LH, Birnstiel ML. Reiteration and clustering of DNA sequences complementary to histone messenger RNA. Nat New Biol. 1971 Apr 7;230(14):165–169. [PubMed]
  • Crawford RJ, Krieg P, Harvey RP, Hewish DA, Wells JR. Histone genes are clustered with a 15-kilobase repeat in the chicken genome. Nature. 1979 May 10;279(5709):132–136. [PubMed]
  • Jacob E. Histone-gene reiteration in the genome of mouse. Eur J Biochem. 1976 May 17;65(1):275–284. [PubMed]
  • Wilson MC, Melli M, Birnstiel ML. Reiteration frequency of histone coding sequences in man. Biochem Biophys Res Commun. 1974 Nov 27;61(2):404–409. [PubMed]
  • Scott AC, Wells JR. Reiteration frequency of the gene for tissue-specific histone H5 in the chicken genome. Nature. 1976 Feb 26;259(5545):635–638. [PubMed]
  • Sures I, Lowry J, Kedes LH. The DNA sequence of sea urchin (S. purpuratus) H2A, H2B and H3 histone coding and spacer regions. Cell. 1978 Nov;15(3):1033–1044. [PubMed]
  • Engel JD, Dodgson JB. Analysis of the closely linked adult chicken alpha-globin genes in recombinant DNAs. Proc Natl Acad Sci U S A. 1980 May;77(5):2596–2600. [PMC free article] [PubMed]
  • Smith HO, Birnstiel ML. A simple method for DNA restriction site mapping. Nucleic Acids Res. 1976 Sep;3(9):2387–2398. [PMC free article] [PubMed]
  • Girvitz SC, Bacchetti S, Rainbow AJ, Graham FL. A rapid and efficient procedure for the purification of DNA from agarose gels. Anal Biochem. 1980 Aug;106(2):492–496. [PubMed]
  • Maniatis T, Jeffrey A, Kleid DG. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. [PMC free article] [PubMed]
  • Maniatis T, Jeffrey A, van deSande H. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975 Aug 26;14(17):3787–3794. [PubMed]
  • Tabak HF, Flavell RA. A method for the recovery of DNA from agarose gels. Nucleic Acids Res. 1978 Jul;5(7):2321–2332. [PMC free article] [PubMed]
  • Herrmann R, Neugebauer K, Pirkl E, Zentgraf H, Schaller H. Conversion of bacteriophage fd into an efficient single-stranded DNA vector system. Mol Gen Genet. 1980 Jan;177(2):231–242. [PubMed]
  • Lacy E, Hardison RC, Quon D, Maniatis T. The linkage arrangement of four rabbit beta-like globin genes. Cell. 1979 Dec;18(4):1273–1283. [PubMed]
  • Dugaiczyk A, Boyer HW, Goodman HM. Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structures. J Mol Biol. 1975 Jul 25;96(1):171–184. [PubMed]
  • Klein RD, Selsing E, Wells RD. A rapid microscale technique for isolation of recombinant plasmid DNA suitable for restriction enzyme analysis. Plasmid. 1980 Jan;3(1):88–91. [PubMed]
  • Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. [PubMed]
  • Long EO, Dawid IB. Repeated genes in eukaryotes. Annu Rev Biochem. 1980;49:727–764. [PubMed]

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