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Proc Natl Acad Sci U S A. Jan 1981; 78(1): 450–453.
PMCID: PMC319071

Rapidly evolving mouse alpha-globin-related pseudo gene and its evolutionary history.

Abstract

The nucleotide sequences of a mouse pseudo alpha-globin gene and two adult alpha-globin genes from mouse and rabbit were compared. A close examination of sequence differences among the three genes revealed that the mouse pseudo alpha-globin gene was derived from one of the mouse alpha-globin genes about 24 million years ago by gene duplication and lost its original function, presumably due to loss of both the intervening sequences or frameshift mutations that prevented production of a functional globin polypeptide, and eventually became an inactive gene about 17 million years ago. After this event, this gene evolved at a very high rate, approximately 1.9 times the rate of synonymous codon change in productive genes. This suggests the presence of functional constraints against synonymous codon changes in normally functioning genes and also suggests that the "nucleotide arrangements" serve almost no important functions beside protein coding ability in the greater part of this gene, except for a very limited number of nucleotides. A continuous stretch of the pseudo alpha-globin gene consisting of a third exon and a 5' half of the 3' noncoding region shows marked sequence homology to the alpha-globin gene, suggesting transfer from one of the globin or globin-like genes by recombination very recently.

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Selected References

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  • Nishioka Y, Leder P. The complete sequence of a chromosomal mouse alpha--globin gene reveals elements conserved throughout vertebrate evolution. Cell. 1979 Nov;18(3):875–882. [PubMed]
  • Konkel DA, Tilghman SM, Leder P. The sequence of the chromosomal mouse beta-globin major gene: homologies in capping, splicing and poly(A) sites. Cell. 1978 Dec;15(4):1125–1132. [PubMed]
  • Konkel DA, Maizel JV, Jr, Leder P. The evolution and sequence comparison of two recently diverged mouse chromosomal beta--globin genes. Cell. 1979 Nov;18(3):865–873. [PubMed]
  • van Ooyen A, van den Berg J, Mantei N, Weissmann C. Comparison of total sequence of a cloned rabbit beta-globin gene and its flanking regions with a homologous mouse sequence. Science. 1979 Oct 19;206(4416):337–344. [PubMed]
  • Hardison RC, Butler ET, 3rd, Lacy E, Maniatis T, Rosenthal N, Efstratiadis A. The structure and transcription of four linked rabbit beta-like globin genes. Cell. 1979 Dec;18(4):1285–1297. [PubMed]
  • Lawn RM, Fritsch EF, Parker RC, Blake G, Maniatis T. The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA. Cell. 1978 Dec;15(4):1157–1174. [PubMed]
  • Dodgson JB, Strommer J, Engel JD. Isolation of the chicken beta-globin gene and a linked embryonic beta-like globin gene from a chicken DNA recombinant library. Cell. 1979 Aug;17(4):879–887. [PubMed]
  • Smithies O, Blechl AE, Denniston-Thompson K, Newell N, Richards JE, Slightom JL, Tucker PW, Blattner FR. Cloning human fetal gamma globin and mouse alpha-type globin DNA: characterization and partial sequencing. Science. 1978 Dec 22;202(4374):1284–1289. [PubMed]
  • Leder A, Miller HI, Hamer DH, Seidman JG, Norman B, Sullivan M, Leder P. Comparison of cloned mouse alpha- and beta-globin genes: conservation of intervening sequence locations and extragenic homology. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6187–6191. [PMC free article] [PubMed]
  • van den Berg J, van Ooyen A, Mantei N, Schamböck A, Grosveld G, Flavell RA, Weissmann C. Comparison of cloned rabbit and mouse beta-globin genes showing strong evolutionary divergence of two homologous pairs of introns. Nature. 1978 Nov 2;276(5683):37–44. [PubMed]
  • Nishioka Y, Leder A, Leder P. Unusual alpha-globin-like gene that has cleanly lost both globin intervening sequences. Proc Natl Acad Sci U S A. 1980 May;77(5):2806–2809. [PMC free article] [PubMed]
  • Vanin EF, Goldberg GI, Tucker PW, Smithies O. A mouse alpha-globin-related pseudogene lacking intervening sequences. Nature. 1980 Jul 17;286(5770):222–226. [PubMed]
  • Heindell HC, Liu A, Paddock GV, Studnicka GM, Salser WA. The primary sequence of rabbit alpha-globin mRNA. Cell. 1978 Sep;15(1):43–54. [PubMed]
  • Miyata T, Yasunaga T, Yamawaki-Kataoka Y, Obata M, Honjo T. Nucleotide sequence divergence of mouse immunoglobulin gamma 1 and gamma 2b chain genes and the hypothesis of intervening sequence-mediated domain transfer. Proc Natl Acad Sci U S A. 1980 Apr;77(4):2143–2147. [PMC free article] [PubMed]
  • Miyata T, Yasunaga T. Molecular evolution of mRNA: a method for estimating evolutionary rates of synonymous and amino acid substitutions from homologous nucleotide sequences and its application. J Mol Evol. 1980 Sep;16(1):23–36. [PubMed]
  • Romero-Herrera AE, Lehmann H, Joysey KA, Friday AE. Molecular evolution of myoglobin and the fossil record: a phylogenetic synthesis. Nature. 1973 Dec 14;246(5433):389–395. [PubMed]
  • Salser W, Isaacson JS. Mutation rates in globin genes: the genetic load and Haldane's dilemma. Prog Nucleic Acid Res Mol Biol. 1976;19:205–220. [PubMed]
  • Grunstein M, Schedl P, Kedes L. Sequence analysis and evolution of sea urchin (Lytechinus pictus and Strongylocentrotus purpuratus) histone H4 messenger RNAs. J Mol Biol. 1976 Jun 25;104(2):351–369. [PubMed]
  • Kimura M. Preponderance of synonymous changes as evidence for the neutral theory of molecular evolution. Nature. 1977 May 19;267(5608):275–276. [PubMed]
  • Kafatos FC, Efstratiadis A, Forget BG, Weissman SM. Molecular evolution of human and rabbit beta-globin mRNAs. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5618–5622. [PMC free article] [PubMed]
  • Salser W. Globin mRNA sequences: analysis of base pairing and evolutionary implications. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):985–1002. [PubMed]
  • Lomedico P, Rosenthal N, Efstratidadis A, Gilbert W, Kolodner R, Tizard R. The structure and evolution of the two nonallelic rat preproinsulin genes. Cell. 1979 Oct;18(2):545–558. [PubMed]
  • Martial JA, Hallewell RA, Baxter JD, Goodman HM. Human growth hormone: complementary DNA cloning and expression in bacteria. Science. 1979 Aug 10;205(4406):602–607. [PubMed]
  • Nichols BP, Yanofsky C. Nucleotide sequences of trpA of Salmonella typhimurium and Escherichia coli: an evolutionary comparison. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5244–5248. [PMC free article] [PubMed]
  • Miyata T, Yasunaga T, Nishida T. Nucleotide sequence divergence and functional constraint in mRNA evolution. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7328–7332. [PMC free article] [PubMed]
  • Kimura M, Ota T. On the stochastic model for estimation of mutational distance between homologous proteins. J Mol Evol. 1972 Dec 29;2(1):87–90. [PubMed]
  • Lai CJ, Khoury G. Deletion mutants of simian virus 40 defective in biosynthesis of late viral mRNA. Proc Natl Acad Sci U S A. 1979 Jan;76(1):71–75. [PMC free article] [PubMed]
  • Cordell B, Bell G, Tischer E, DeNoto FM, Ullrich A, Pictet R, Rutter WJ, Goodman HM. Isolation and characterization of a cloned rat insulin gene. Cell. 1979 Oct;18(2):533–543. [PubMed]
  • Bell GI, Pictet RL, Rutter WJ, Cordell B, Tischer E, Goodman HM. Sequence of the human insulin gene. Nature. 1980 Mar 6;284(5751):26–32. [PubMed]
  • Grantham R, Gautier C, Gouy M. Codon frequencies in 119 individual genes confirm consistent choices of degenerate bases according to genome type. Nucleic Acids Res. 1980 May 10;8(9):1893–1912. [PMC free article] [PubMed]
  • Miyata T, Hayashida H, Yasunaga T, Hasegawa M. The preferential codon usages in variable and constant regions of immunoglobulin genes are quite distinct from each other. Nucleic Acids Res. 1979 Dec 20;7(8):2431–2438. [PMC free article] [PubMed]
  • Kimura M. Evolutionary rate at the molecular level. Nature. 1968 Feb 17;217(5129):624–626. [PubMed]

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