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Nucleic Acids Res. May 11, 1984; 12(9): 3983–4000.
PMCID: PMC318805

Molecular analysis of the alcohol dehydrogenase (Adh1) gene of maize.


A cDNA clone of maize Adh1 which contains the entire protein coding region of the gene has been constructed. The protein sequence predicted from the nucleotide sequence is in agreement with limited protein sequencing data for the ADH1 enzyme. An 11.5 kb genomic fragment containing the Adh1 gene has been isolated using the cDNA clone as a probe, and the gene region fully sequenced. The gene is interrupted by 9 introns, their junction sequences fitting the animal gene consensus sequence. Within the gene there is a triplication of a segment (104 bp) spanning an intron-exon junction. Presumptive promoter elements have been identified and are similar in nucleotide sequence and location, relative to the start of transcription, to those of other plant and animal genes. No recognizable poly(A+) addition signal is evident. Comparison of the nucleotide sequences of the cDNA (derived from an Adh1 -F allele) and genomic (derived from an Adh1 -S allele) clones has identified an amino acid difference consistent with the observed difference in electrophoretic mobility of the two enzymes. The maize ADH1 amino acid sequence is 50% homologous to that of horse liver ADH but is only 20% homologous to yeast ADH.

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Selected References

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  • Sachs MM, Freeling M, Okimoto R. The anaerobic proteins of maize. Cell. 1980 Jul;20(3):761–767. [PubMed]
  • Gerlach WL, Pryor AJ, Dennis ES, Ferl RJ, Sachs MM, Peacock WJ. cDNA cloning and induction of the alcohol dehydrogenase gene (Adh1) of maize. Proc Natl Acad Sci U S A. 1982 May;79(9):2981–2985. [PMC free article] [PubMed]
  • Schwartz D, Endo T. Alcohol Dehydrogenase Polymorphism in Maize-simple and Compound Loci. Genetics. 1966 Apr;53(4):709–715. [PMC free article] [PubMed]
  • Chandler PM, Rimkus D, Davidson N. Gel electrophoretic fractionation of RNAs by partial denaturation with methylmercuric hydroxide. Anal Biochem. 1979 Oct 15;99(1):200–206. [PubMed]
  • Seed B. Diazotizable arylamine cellulose papers for the coupling and hybridization of nucleic acids. Nucleic Acids Res. 1982 Mar 11;10(5):1799–1810. [PMC free article] [PubMed]
  • Karn J, Brenner S, Barnett L, Cesareni G. Novel bacteriophage lambda cloning vector. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5172–5176. [PMC free article] [PubMed]
  • Benton WD, Davis RW. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. [PubMed]
  • Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Messing J, Vieira J. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982 Oct;19(3):269–276. [PubMed]
  • Weaver RF, Weissmann C. Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates. Nucleic Acids Res. 1979 Nov 10;7(5):1175–1193. [PMC free article] [PubMed]
  • Cleveland DW, Fischer SG, Kirschner MW, Laemmli UK. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed]
  • Kelly J, Freeling M. Purification of maize alcohol dehydrogenase-1 allozymes and comparison of their tryptic peptides. Biochim Biophys Acta. 1980 Jul 24;624(1):102–110. [PubMed]
  • Shine J, Dalgarno L. Determinant of cistron specificity in bacterial ribosomes. Nature. 1975 Mar 6;254(5495):34–38. [PubMed]
  • Breathnach R, Chambon P. Organization and expression of eucaryotic split genes coding for proteins. Annu Rev Biochem. 1981;50:349–383. [PubMed]
  • Kozak M. Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Res. 1981 Oct 24;9(20):5233–5252. [PMC free article] [PubMed]
  • Freeling M. Allelic variation at the level of intragenic recombination. Genetics. 1978 May;89(1):211–224. [PMC free article] [PubMed]
  • Schwartz D. Genetic control of alcohol dehydrogenase--a competition model for regulation of gene action. Genetics. 1971 Mar;67(3):411–425. [PMC free article] [PubMed]
  • Pedersen K, Devereux J, Wilson DR, Sheldon E, Larkins BA. Cloning and sequence analysis reveal structural variation among related zein genes in maize. Cell. 1982 Jul;29(3):1015–1026. [PubMed]
  • Wieringa B, Meyer F, Reiser J, Weissmann C. Unusual splice sites revealed by mutagenic inactivation of an authentic splice site of the rabbit beta-globin gene. Nature. 1983 Jan 6;301(5895):38–43. [PubMed]
  • Pelham HR. A regulatory upstream promoter element in the Drosophila hsp 70 heat-shock gene. Cell. 1982 Sep;30(2):517–528. [PubMed]
  • Eklund H, Brändén CI, Jörnvall H. Structural comparisons of mammalian, yeast and bacillar alcohol dehydrogenases. J Mol Biol. 1976 Mar 25;102(1):61–73. [PubMed]
  • Eklund H, Nordström B, Zeppezauer E, Söderlund G, Ohlsson I, Boiwe T, Söderberg BO, Tapia O, Brändén CI, Akeson A. Three-dimensional structure of horse liver alcohol dehydrogenase at 2-4 A resolution. J Mol Biol. 1976 Mar 25;102(1):27–59. [PubMed]

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