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PMC full text:
Biochim Biophys Acta. Author manuscript; available in PMC 2012 Nov 1.
Published in final edited form as:
Biochim Biophys Acta. 2011 Nov; 1812(11): 1393–1402.
Published online 2011 Jul 21. doi:  10.1016/j.bbadis.2011.07.007

Figure 5

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Glucose and fetal bovine serum concentrations effects on temperature sensitive growth and phospholipid biosynthesis of GroD1 cells

(A) Coomassie blue colony staining of GroD1 cells grown in media containing the indicated amounts of glucose and FBS at 40°C for 14 days. Increasing amounts of FBS, as well as lower concentration of glucose allowed GroD1 growth at the restrictive temperatures. (B) Phospholipid biosynthesis of GroD1 cells at the indicated glucose and FBS concentrations. Cells were labeled with 32Pi for 2 h at 40°C, lipids were extracted, separated and quantified as described in Material and Methods. The horizontal bars represents expected values for parent strain ZR-82. All glucose values took into account the contribution of the fetal bovine serum used to supplement the medium which contains 8 mM glucose. (C) Fluorescent measurement of cellular glucose-6-phosphate (G6P) and fructose-6-phosphate (F6P) cellular levels. Arrows on the tracing indicate that a bolus of 300 pmol of G6P or F6P standard was added. The addition of G6PDH and GPI are indicated on the X axis. Insert: Quantitated metabolites levels. All values represent the average ± standard deviation of three independent experiments. * Indicates a P ≤ 0.05 between samples.

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