Logo of narLink to Publisher's site
Nucleic Acids Res. 1989 Jul 25; 17(14): 5713–5723.
PMCID: PMC318191

Fast light-regulated genes of Neurospora crassa.

Abstract

Several physiological reactions including the sexual differentiation of the ascomycete Neurospora crassa are triggered by blue light. Mutants in the white-collar genes wc-1 and wc-2 are blind for all the blue light effects tested so far. We have previously shown that blue light induces some translatable mRNAs at different times after beginning the illumination. Here we report the cDNA cloning of four genes that are induced by blue light. Induction of these transcripts is temporally ordered (lag times from 2 to 45 min). Analysis of run-on transcripts show that the increases in mRNA levels are due to de novo transcription. None of these transcripts is inducible in white-collar mutants.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Kreuzaler F, Ragg H, Fautz E, Kuhn DN, Hahlbrock K. UV-induction of chalcone synthase mRNA in cell suspension cultures of Petroselinum hortense. Proc Natl Acad Sci U S A. 1983 May;80(9):2591–2593. [PMC free article] [PubMed]
  • Nagy F, Kay SA, Chua NH. Gene regulation by phytochrome. Trends Genet. 1988 Feb;4(2):37–42. [PubMed]
  • Fromm H, Devic M, Fluhr R, Edelman M. Control of psbA gene expression: in mature Spirodela chloroplasts light regulation of 32-kd protein synthesis is independent of transcript level. EMBO J. 1985 Feb;4(2):291–295. [PMC free article] [PubMed]
  • Gerdes HH, Kindl H. Gene response upon illumination in forming mRNA encoding peroxisomal glycollate oxidase. Biochim Biophys Acta. 1988 Feb 28;949(2):195–205. [PubMed]
  • Chambers JA, Hinkelammert K, Russo VE. Light-regulated protein and poly(A)+ mRNA synthesis in Neurospora crassa. EMBO J. 1985 Dec 30;4(13B):3649–3653. [PMC free article] [PubMed]
  • Harding RW, Melles S. Genetic Analysis of Phototropism of Neurospora crassa Perithecial Beaks Using White Collar and Albino Mutants. Plant Physiol. 1983 Aug;72(4):996–1000. [PMC free article] [PubMed]
  • Sargent ML, Briggs WR. The effects of light on a circadian rhythm of conidiation in neurospora. Plant Physiol. 1967 Nov;42(11):1504–1510. [PMC free article] [PubMed]
  • Russo VE. Blue light induces circadian rhythms in the bd mutant of Neurospora: double mutants bd,wc-1 and bd,wc-2 are blind. J Photochem Photobiol B. 1988 Jul;2(1):59–65. [PubMed]
  • Harding RW, Turner RV. Photoregulation of the Carotenoid Biosynthetic Pathway in Albino and White Collar Mutants of Neurospora crassa. Plant Physiol. 1981 Sep;68(3):745–749. [PMC free article] [PubMed]
  • Nelson MA, Morelli G, Carattoli A, Romano N, Macino G. Molecular cloning of a Neurospora crassa carotenoid biosynthetic gene (albino-3) regulated by blue light and the products of the white collar genes. Mol Cell Biol. 1989 Mar;9(3):1271–1276. [PMC free article] [PubMed]
  • Degli-Innocenti F, Russo VE. Isolation of new white collar mutants of Neurospora crassa and studies on their behavior in the blue light-induced formation of protoperithecia. J Bacteriol. 1984 Aug;159(2):757–761. [PMC free article] [PubMed]
  • Boll W, Fujisawa J, Niemi J, Weissmann C. A new approach to high sensitivity differential hybridization. Gene. 1986;50(1-3):41–53. [PubMed]
  • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. [PubMed]
  • Willmitzer L, Wagner KG. The isolation of nuclei from tissue-cultured plant cells. Exp Cell Res. 1981 Sep;135(1):69–77. [PubMed]
  • Wong LJ, Marzluf GA. Sequence complexity and abundance classes of nuclear and polysomal polyadenylated RNA in Neurospora crassa. Biochim Biophys Acta. 1980 Mar 28;607(1):122–135. [PubMed]
  • Darnell JE., Jr Variety in the level of gene control in eukaryotic cells. Nature. 1982 Jun 3;297(5865):365–371. [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

Formats:

Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...