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J Bacteriol. Apr 1966; 91(4): 1520–1525.
PMCID: PMC316071

Induction of Cellular Morphogenesis in Myxococcus xanthus II. Macromolecular Synthesis and Mechanism of Inducer Action

Abstract

Sadler, William (University of Minnesota, Minneapolis), and Martin Dworkin. Induction of cellular morphogenesis in Myxococcus xanthus. II. Macromolecular synthesis and mechanism of inducer action. J. Bacteriol. 91:1520–1525. 1966.—Net changes in ribonucleic acid (RNA), deoxyribonucleic acid (DNA), and protein syntheses in cells of Myxococcus xanthus during induced, synchronous conversion to microcysts are described. The net synthesis of all three macromolecules was temporarily halted for a brief period during the initiation of shape change. Synthesis then resumed and leveled off when refractile microcysts began to appear. The conversion was completely sensitive, throughout the process, to low concentrations of chloramphenicol and actinomycin D. The uptake of amino acids and uracil was linear throughout the conversion, suggesting that the plateaus in rates of net synthesis of protein and RNA represented a period of rapid turnover. The most effective inducers of microcyst formation were fully saturated aliphatic compounds containing 2 to 4 carbon atoms and at least one primary or secondary alcohol group. Studies with labeled inducer indicated that the inducer need not be taken up by the cells to be effective, and probably interacts with some peripheral structure of the cell. The possibility that induction involves an alteration of a membrane-DNA complex is discussed.

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Selected References

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  • DWORKIN M, GIBSON SM. A SYSTEM FOR STUDYING MICROBIAL MORPHOGENESIS: RAPID FORMATION OF MICROCYSTS IN MYXOCOCCUS XANTHUS. Science. 1964 Oct 9;146(3641):243–244. [PubMed]
  • Dworkin M, Sadler W. Induction of cellular morphogenesis in Myxococcus xanthus. I. General description. J Bacteriol. 1966 Apr;91(4):1516–1519. [PMC free article] [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
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  • TREICK RW, KONETZKA WA. PHYSIOLOGICAL STATE OF ESCHERICHIA COLI AND THE INHIBITION OF DEOXYRIBONUCLEIC ACID SYNTHESIS BY PHENETHYL ALCOHOL. J Bacteriol. 1964 Dec;88:1580–1584. [PMC free article] [PubMed]

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