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Curr Opin Cell Biol. Author manuscript; available in PMC Aug 1, 2012.
Published in final edited form as:
PMCID: PMC3148286
NIHMSID: NIHMS295136

Lipid transfer and signaling at organelle contact sites: the tip of the iceberg

Abstract

Membrane contact sites (MCSs) are formed by the close apposition of membranes of two organelles. They are zones where signals and small molecules, such as lipids and calcium, are exchanged between intracellular compartments. The past few years have seen considerable progress in our understanding of how MCSs form and facilitate the exchange of lipids and signals. Here we summarize what has been learned about MCSs between the endoplamic reticulum (ER) and the plasma membrane, the ER and mitochondria, and the ER and endosomes or lysosomes. These findings suggest that we are just beginning to understand how MCSs form and function.

Introduction

Compartmentalization in eukaryotic cells induces the need for inter-organelle trafficking of information and metabolites such as lipids. Mechanisms of intracellular lipid exchange are summarized in Box 1. One location where lipids, other small molecules, and signals are exchanged between organelles is membrane contact sites (MCSs), regions where the membranes of two organelles are closely apposed [1, 2]. At these sites the membranes of two organelles come within 10 to 30 nm of one another. MCSs are ubiquitous in all cells types. Many are observed between the endoplasmic reticulum (ER) and a second organelle (Figure 1). MCSs have also been observed within organelles that contain internal membranes such as mitochondria, chloroplasts, and multivesicular bodies. In the past few years there has been considerable progress in our understanding of how small molecules, such as lipids and calcium, and signals are exchanged at MCS as well as how some MCSs are formed. Here we focus on recent advances in our knowledge of lipid trafficking and signaling at MCSs between the ER and other organelles, and also focus on how MCSs are formed. For reviews of calcium trafficking at these sites see [3, 4].

Box 1

Mechanisms of intracellular lipid exchange

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Lipid exchange between cellular compartments occurs by both vesicular and non-vesicular mechanisms, [52, 53]. One mechanism of non-vesicular lipid transfer between membranes is the spontaneous transfer of lipid monomers (B). This process is too slow to be physiologically relevant for most classes of lipid, with the notable exceptions of sterols and some lysolipids. Some non-vesicular lipid exchange requires lipid-transfer proteins (LTPs) (C). There are five large families of LTPs and most cell types express many of them [54]. LTPs bind a single lipid molecule in a hydrophobic tunnel or cleft and can shuttle the bound lipid between membranes. Rather than diffusing long distances through the cytosol many LTPs may function at MCSs, where they could rapidly shuttle between membranes (D-F). Some lipid exchange at MCS does not seem to require LTPs but instead may be mediated by integral membrane transporters (G).

Figure 1
Location of membrane contact sites

MCSs between the ER and the plasma membrane (PM)

Close contacts between the ER and PM have been observed in all cells and can be quite extensive; such as in muscle cells where these contacts are needed for calcium exchange between these organelles.

How close contacts between the ER and PM are established is not well understood. Several proteins that regulate ER-PM contact site formation have been identified. In mammalian cells the ER calcium sensor STIM1 and the PM calcium channel ORAI1 interact when cells are depleted of calcium and drive the formation of ER-PM contacts [3, 5]. In muscle cells, junctophilins are thought to maintain close contacts and perhaps a specific spacing between the ER and PM. These integral membrane proteins reside in the ER and probably simultaneously bind the PM [6, 7]. Another mechanism for induction of ER-PM contacts has recently been suggested [8]. This work showed that dimerization of a coatomer-binding peptide anchored to ER membranes induced ER-PM contacts in a microtubule dependent fashion [8]. Although the mechanism is not yet understood, this work suggests that vesicular trafficking may be required to bring the ER close to the PM. It is not clear if simply bringing the ER near the PM is enough to cause contacts to form or if other steps are necessary. In yeast the ER proteins Ice2 and Scs2 are thought to be important for ER-PM contacts since cells missing both proteins have a significant decrease in the amount of ER found closely apposed to the PM [9].

There is growing evidence that sterols and signals are exchanged between the ER and PM at close contacts between these organelles. It has been known for some time that sterols can be transferred between the ER and PM by non-vesicular pathways [10-13]. In yeast, this sterol exchange between the ER and PM may be mediated by oxysterol-binding protein (OSBP)-related proteins (ORPs), lipid-binding proteins conserved from yeast to humans [14, 15]. Yeast has seven ORPs, which are called Osh proteins, and it has been found that all of them can bind sterols and exchange them between liposomes in vitro [16••]. The structure of one of these proteins, Osh4/Kes1, has been solved and found to form a nearly complete β-barrel that binds a single sterol inside [17]. In a strain missing all the Osh proteins sterol exchange between the ER and PM has been shown to slow significantly [18]. However, whether Osh proteins mediate most of the non-vesicular sterol exchange between the ER and PM or other compartments has been questioned [19]. In mammalian cells, recent work implicates some mammalian ORPs in non-vesicular sterol trafficking from the PM to the ER and lipid droplets (LDs) [20•] and other ORPs have been shown to bind and transfer cholesterol in vitro [21].

Four of the seven Osh protein are enriched on regions of the ER that are close to the PM, presumably at ER-PM contact sites [16••]. Two of these Osh proteins, Osh2 and Osh3, have domains that are required for targeting them to ER-PM contact sites [22, 23]. They contain a pleckstrin homology (PH) domain, which binds phosphoinositides (PIPs) in the PM and a two phenylalanines in an acidic tract (FFAT) motif, which is bound by the ER membrane proteins Scs2 and Scs22. The mammalian homologues of Scs2 and Scs22 are known as VAMP-associated proteins (VAPs). The PH and FFAT domains of Osh proteins are required for targeting them to ER-PM contact sites. However, two of the Osh protein that localize to ER-PM contacts lack PH and FFAT motifs and it is not known how they are targeted to these sites.

The core lipid-binding domain found in all Osh proteins has the ability to bind two membranes simultaneously [16••]. Membrane binding by this domain was studied in Osh4/Kes1 and was found to have at least two membrane-binding surfaces, one near the mouth of the sterol-binding pocket and a second at the distal site. The distal binding surface is required for Osh4/Kes1 to function in cells and is close to residues needed for PIP binding on the surface of the protein [24] that is not part of the sterol-binding pocket. PIP-binding at this site also regulates sterol binding. Thus, Osh proteins and perhaps all ORPs may be able to bind PIPs (or other lipids) in one membrane while simultaneously extracting or delivering sterols to a second membrane. This makes them well suited to transfer lipids or signals at MCSs.

A new model of how Osh proteins mediate signaling at ER-PM MCSs has been suggested by recent work from Stephan et al [25••]. The authors propose that Osh proteins function as PIP sensors that modulate PIP levels in cells by regulating the PIP phosphatase Sac1. Sac1 is an integral membrane protein in the ER and its active site is in the cytosol. Osh3 interacts with Sac1 at ER-PM contacts sites where it regulates Sac1 activity. Remarkably, this regulation can be reconstituted in vitro and Sac1 in one membrane can dephosphorylate PIPs in a second membrane. Osh proteins and the VAP homologues Scs2 and Scs22 regulate this process. Thus, Sac1 in the ER can hydrolyse PIPs on the PM or at any other MCS between the ER and a second membrane. This conclusion is consistent with a recent structural study of Sac1 [26] and earlier studies that suggested Sac1 can regulate PIP levels in the PM even though it is anchored in the ER [27, 28]. A role for Osh proteins in regulating PIP metabolism has also been found in other studies [24, 29]. How Osh proteins regulate Sac1, what role sterol-binding plays, and what signaling pathways are involved remain to be discovered.

Another method of signaling between the ER and PM at contact sites has been suggested by studies on the tyrosine phosphatase PTP1B. This enzyme is anchored to the cytosolic surface of the ER. A number of studies have reported that PTP1B directly binds signaling substrates on the PM including PKC[partial differential], insulin receptor, EGFR and EphA3 [30-32]. Thus PTP1B probably functions at ER-PM contact sites.

MCSs between ER and mitochondria

Close contacts between the ER and mitochondria have been noted in a number of studies. For example, it has been found that in HeLa cells 5 to 20% of the surface of the mitochondrial network is in contact with the ER [33]. Similarly, a study in yeast found about 100 close contacts (< 30 nm) between the ER and mitochondria per cell [34]. These contacts are thought to be important for the exchange of calcium [4] and lipids [35] between the ER and mitochondria.

In the last few years there has been substantial progress in our understanding of how MCSs between the ER and mitochondria are formed. In mammalian cells the dynamin-like GTPase Mitofusin 2 (Mfn2), which is involved in mitochondrial fusion, has been suggested by the Scorrano group to tether the ER and mitochondria [36•]. Most Mfn2 is localized to the outer mitochondrial membrane (OMM) but the authors found that a fraction was found in the ER. This ER-localized fraction of Mfn2 forms dimers with Mfn2 in the OMM and with Mitofusin 1, which is also in the OMM, tethering ER and mitochondria. Importantly, in Mfn2-/- cells calcium trafficking between the ER and mitochondria is reduced, consistent with a role for Mfn2 in tethering. Recent work has suggested that tethering is also regulated by trichloplein/mitostatin, a keratin-binding protein [37].

A different complex involved in maintaining contacts between the ER and mitochondria in yeast has been identified. Kornmann and colleagues devised a cleaver screen to isolate mutants with a defect in ER-mitochondrial tethering. They discovered a complex that was named ERMES (ER-mitochondria encounter structure) [38••]. This complex contains the ER protein Mmm1, two proteins in the OMM, Mdm10 and Mdm34, and the cytosolic protein Mdm12. Deletion of any one of these proteins causes the complex to dissociate. Consistent with a role for the ERMES complex in tethering, lipid exchange between the ER and mitochondria was found to decrease in ERMES mutants. Whether the ERMES complex is only a tether or participates more directly in lipid exchange between the ER and mitochondria remains an open question. Interestingly, three of the four ERMES complex proteins contain a domain predicted to form a tubular structure that may allow lipid exchange between membranes [39, 40].

MCSs between ER and lysosomes or endosomes

In yeast, formation of the MCS between the nucleus and the vacuole, called the nucleus-vacuole junction (NVJ), requires the interaction of the ER membrane protein Nvj1 with Vac8, which is anchored on the vacuole [41]. NVJ formation is necessary for microautophagy of the nucleus (PMN), which can be induced by stress conditions such as nitrogen starvation or the presence of rapamycin [42]. Whether the NVJ is also a site where lipids or signals are exchanged between the ER and vacuole is not known. The lipid-binding proteins Osh1/Swh1, which is an ORP, and the fatty acid elongase Tsc13 localize to the NVJ and both are involved in PMN formation [43, 44]. Recent findings from the Mayer group have revealed that sterol and sphingolipid synthesis mutants have a defect in PMN formation [45•]. They suggest that a lipid microdomain is present in the membranes of the NVJ and that this microdomain is necessary to recruit proteins to the NVJ for the PMN formation [45•].

The NVJ is not found in higher eukaryotes. However, a number of recent studies in mammalian cells suggest the importance of MCSs between the ER and organelles in the endocytic trafficking pathway such as lysosomes, endosomes, and multivesicular bodies (MVBs). The ER tyrosine phosphatase PTP1B was shown to interact with one of its substrates, EGFR, on the surface of MVBs and localize to close contacts between the ER and MVBs [46]. A second example of signaling between the ER and the endomembrane system is provided by elegant studies on ORP1L, which demonstrate how this protein regulates MCSs between the ER and endosomes [47, 48]. ORP1L binds the small GTPase Rab7 and Rab7-interacting lysosomal protein on endosomes [47]. This association recruits the p150Glued subunit of the dynein-dynactin motor to endosomes [48]. Under low cholesterol conditions, ORP1L undergoes a conformational change that allows its FFAT domain to interact with VAP proteins on the ER, leading to the release of p150Glued and to the formation of close contacts between the ER and endosomes [49••]. Therefore, ORP1L acts as a cholesterol sensor that regulates contacts between the ER and endosomes. Whether cholesterol exchange occurs at these junctions and what role, if any, ORP1L plays in this transfer remains to be determined.

Another ORP, ORP5, has been recently implicated in cholesterol exchange between late endosomes/lysomes (LE/LY) and the ER, perhaps at MCSs between these organelles. Efficient trafficking of cholesterol derived from low-density lipoproteins (LDLs) out of LE/LYs requires Neimann Pick Type C protein 1 (NPC1), an integral membrane protein in LE/LYs [50]. Yang and colleagues found that ORP5 interacts with NPC1 and is needed for trafficking of LDL-derived cholesterol out of LE/LYs [51•]. ORP5 is an integral membrane protein in the ER and may be enriched at MCSs between the ER and LE/LYs. ORP5 is able to transfer sterol between liposomes in vitro and could mediate cholesterol transfer between the ER and LE/LYs [51•]. Alternatively, its primary function may be to regulate MCSs formation between the ER and LE/LYs.

Conclusion

The past few years have seen a dramatic increase in our knowledge of how MCSs are formed and how lipids and signals can be exchanged between organelles at these sites, yet much remains to be elucidated. MCSs almost certainly play critical roles in organelle function. For example, contacts between lipid droplets (LDs) and ER-LD MCS are thought to be important sites of lipid exchange and signaling. How MCSs form and are regulated remain important questions. Some MCSs, such as those between the ER and mitochondria, may be maintained by a number of low affinity interactions rather than by a single complex. Better techniques for isolating and characterizing proteins in MCSs will help resolve this issue. In many cases, the mechanisms of lipid exchange and signaling at MCSs remain poorly understood as does the energetics and regulation of these processes. This minireview has focused on only a few of the known MCSs in cells. Given the large number and diversity of these contacts it is likely that what we know now is only the tip of the iceberg. The next few years are likely to see a revolution in our understanding of how MCSs mediate intracellular trafficking of lipids, calcium, and signals between intracellular compartments.

Acknowledgments

We thank Chris Stefan for sharing unpublished findings, Ashley Slack for reading the manuscript and apologize to colleagues whose work we could not included because of space limitations.

This work was supported by the Intramural Research Fund of the National Institute of Diabetes and Digestive and Kidney diseases.

Footnotes

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