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Figure 4

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Fibronectin receptor mRNA expression is a function of substrate stiffness only at low cell density. VSMCs were seeded onto 25, 70, and 135 kPa polyacrylamide substrates, maintained in 10% FBS depleted of Fn, and lysed for mRNA extraction after 96–120 h in culture. Each value represents an average of 2–7 biological replicates, with each sample assayed 2–3 times with qPCR.

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