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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Ann Rheum Dis. Author manuscript; available in PMC Jul 13, 2011.
Published in final edited form as:
PMCID: PMC3135414
NIHMSID: NIHMS302721

Association of IRF5 polymorphisms with activation of the interferon α pathway

Abstract

Objective

The genetic association of interferon regulatory factor 5 (IRF5) with systemic lupus erythematosus (SLE) susceptibility has been convincingly established. To gain understanding of the effect of IRF5 variation in individuals without SLE, a study was undertaken to examine whether such genetic variation predisposes to activation of the interferon α (IFNα) pathway.

Methods

Using a computer simulated approach, 14 single nucleotide polymorphisms (SNPs) and haplotypes of IRF5 were tested for association with mRNA expression levels of IRF5, IFNα and IFN-inducible genes and chemokines in lymphoblastoid cell lines (LCLs) from individuals of European (CEU), Han Chinese (CHB), Japanese (JPT) and Yoruba Nigerian (YRI) backgrounds. IFN-inducible gene expression was assessed in LCLs from children with SLE in the presence and absence of IFNα stimulation.

Results

The major alleles of IRF5 rs13242262 and rs2280714 were associated with increased IRF5 mRNA expression levels in the CEU, CHB+JPT and YRI samples. The minor allele of IRF5 rs10488631 was associated with increased IRF5, IFNα and IFN-inducible chemokine expression in CEU (pc=0.0005, 0.01 and 0.04, respectively). A haplotype containing these risk alleles of rs13242262, rs10488631 and rs2280714 was associated with increased IRF5, IFNα and IFN-inducible chemokine expression in CEU LCLs. In vitro studies showed specific activation of IFN-inducible genes in LCLs by IFNα.

Conclusions

SNPs of IRF5 in healthy individuals of a number of ethnic groups were associated with increased mRNA expression of IRF5. In European-derived individuals, an IRF5 haplotype was associated with increased IRF5, IFNα and IFN-inducible chemokine expression. Identifying individuals genetically predisposed to increased IFN-inducible gene and chemokine expression may allow early detection of risk for SLE.

INTRODUCTION

A major breakthrough in the pathogenesis of systemic lupus erythematosus (SLE) is the role of interferon α (IFNα) pathway activation,1,2 a common heritable risk factor for SLE.3 IFNα, part of the innate immune response, functions in autoimmunity by promoting dendritic cell maturation, T cell survival and antibody and cytokine production.46 Variants of the IFNα pathway genes interferon regulatory factor 5 (IRF5), tyrosine kinase 2 (TYK2), STAT4 and osteopontin (OPN) have been associated with SLE susceptibility in multiple ethnic groups,720 but the complete impact of genetic variation on pathway activation is not fully understood. IRF5 has been repeatedly implicated in susceptibility to many other autoimmune diseases including rheumatoid arthritis,2123 multiple sclerosis,24 inflammatory bowel disease25 and Sjogren’s syndrome,26 which suggests a critical and common function in the promotion of autoimmunity and a rationale for the focus of our study.

Increased IFNα-inducible gene expression is observed in peripheral blood cells from patients affected with a number of autoimmune diseases including polymyositis and dermatomyosits,27,28 suggesting a common role for the IFN pathway. Interestingly, high serum IFNα activity is not associated with ANA or anti-SSA/Ro antibody positivity in subjects without disease manifestations,29,30 suggesting these are independent variables in the development of SLE. The correlation between IFN-inducible gene expression in peripheral blood cells and lupus disease activity and fl are,3136 however, implicates the IFNα pathway specifically in the development and exacerbation of SLE.

A focus of SLE research has been on downstream activation of IFN-inducible genes as representative of IFNα activity. In particular, interferon scores, derived from significantly increased IFN-inducible genes, have been associated with SLE subpopulations with increased disease activity.34,36 These interferon scores in a functional assay have been used to identify an IRF5 SLE risk haplotype associated with high IFNα activation.29 Similarly, chemokines that are upregulated by IFNα have been described as measures of IFNα pathway activation and increased SLE disease activity, including neuropsychiatric SLE.32,37

This project expands current understanding of IRF5 variations by studying basal gene transcript levels representing IFNα pathway activation. Important resources including genotyping data provided by Hapmap38 and gene expression data from NCBI Gene Expression Omnibus (GEO) were used in the analysis. Our purpose is to examine whether genetic variation of IRF5 predisposes individuals without autoimmune disease to IFNα pathway activation by analysing gene expression levels of IFNα and IFN-inducible genes and chemokines. Greater understanding of the complex genetics influencing the IFNα pathway may lead to future developments such as autoimmune risk prediction models.

MATERIALS AND METHODS

Sources of data

The Hapmap Project (www.hapmap.org) provided complete genotyping data of IRF5 (±5kb) from all unrelated individuals including 60 individuals of European background (CEU), 45 Han Chinese (CHB), 45 Japanese (JPT) and 60 with a Yoruba Nigerian (YRI) background (phenotypic data unknown). Of the total 18 single nucleotide polymorphisms (SNPs), 14 with minor allele frequency (MAF) >0.025 were used for analyses.

IRF5, the 13 IFNα subtypes and specific IFN-inducible gene including chemokine expression levels from Epstein-Barr virus (EBV)-immortalised lymphoblastoid cell lines (LCLs) of Hapmap individuals obtained from the GEO repository (GSE5859 for CEU, CHB and JPT; GSE7761 for YRI)39,40 were used to generate and test association hypotheses. Only individuals whose gene expression was not available were excluded.

Interferon and chemokine scores, gene expression and haplotypes

For each individual, two interferon scores, one chemokine score and one total IFNα expression sum were calculated by summing normalised mean expression levels of relevant genes. The first IFN score (IFN score 1) consisted of summed gene expression of LY6E, OASL, OAS1, MX-1 and ISG15, which were previously identified by our group as explaining 98% of variation in differential quantitative IFN-inducible gene expression between patients with SLE and controls.34

The second IFN score (IFN score 2) consisted of summed gene expression of PRKR, IFIT1 and IFI44, three genes that are preferentially induced by IFNα (but not IFNγ) in microarray analysis of patients with SLE versus controls.35,36 The CMK score was calculated by summing gene expression of CXCL10, CCL2 and CCL9, the three IFN-inducible chemokines that have highest correlation with SLE disease activity.32,41 Total IFNα expression was calculated by summing gene expression of the 13 IFNα subtypes.

Statistical inference of haplotypes from the unphased Hapmap SNP genotypes was required. Individual haplotypes were deduced with Haplotyper 1.0 using the Bayesian algorithm. Resolved haplotypes were divided into blocks (Haploviewer 4.0) using the approach of CI.

Lymphoblastoid cell line in vitro stimulation and mRNA quantification

EBV-transformed LCLs of children with SLE were suspended in AIM V (Invitrogen) and supplemented with 15% fetal bovine serum. LCL cells (2×106 cells/ml) were incubated (24 h) in complete medium with 600 units/ml of pure recombinant human IFNα (Invitrogen). An aliquot of 0.25 μg/ml of recombinant B18R (eBioscience), a type I IFN receptor protein, was added to some wells as a neutralising agent.42 RNA was extracted (Trizol) and first-strand cDNA was synthesised from 2 μg RNA template using Omniscript reverse transcriptase (Qiagen) and oligo(dT) 20 primers (Invitrogen). Transcript levels were quantified in each sample using the AB 7500 RT-PCR System under standard thermal cycling conditions. A mean threshold cycle for each sample in two replicates was used to calculate the relative expression of IFN-inducible genes compared with housekeeping gene human transcripts used as endogenous references.

Statistical analysis

CEU and YRI consisted of unrelated individuals and were analysed as independent samples. CHB and JPT data were combined for analysis as, in previous studies, expression levels of >99% of over 4000 genes have been shown to be similar between these two groups.40

Two well correlated (r=0.5–0.63; p<0.001, Pearson test) microarray gene expression data sets were analysed using SAS Software (www.sas.com). Equality of variances was tested by the Folded F method. Genotypes of SNPs were assessed by the Student unpaired t test of dominant models (major allele homozygotes vs minor allele homozygotes plus heterozygotes) for association with IRF5 expression and total IFNα expression; other than SNP 2 (table 1), the studied SNPs had the same minor allele in all population samples. SNPs associated with IRF5 and IFNα expression were tested for association with IFN and CMK scores using the same methods. For data that did not meet the assumption of normality, the non-parametric Wilcoxon rank sum test was used. Only SNPs that withstood Bonferroni correction are reported.

Table 1
Association of IRF5 SNPs with IRF6 expression in multiple ethnic groups

Stratification for haplotype analysis was based on haplotypes containing risk alleles associated with increased gene expression. Haplotypes were tested for association by the Student unpaired t test with the gene’s own expression, total IFNα expression, and IFN and CMK scores. Statistical significance of the expression in LCLs was tested by the Student t test.

RESULTS

Association of SNPs of IRF5 with increased IRF5 expression in multiple ethnic groups

Six of the 14 SNPs (figure 1A) are intronic; the others are located at the 5′ upstream or 3′ downstream regions. Introns and 5′ and 3′ regions often contain sequences that influence mRNA expression and are thus considered important for our hypothesis. There were seven tagSNPs in the CEU and the CHB+JPT samples, and eight in the YRI, with most r2 values below 0.8 (figure 1B). Based on tagSNP genotyping availability, it is likely that a large fraction of genetic variation in the IRF5 locus has been adequately covered by linkage disequilibrium (LD) and determined with a minimum of redundancy.

Figure 1
Interferon regulatory factor 5 (IRF5) gene structure and haplotypic architecture

SNPs were individually tested for association with IRF5 mRNA expression. Eight of 11 (73%) in CEU individuals, 7 of 9 (78%) in CHB+JPT, but only 2 of 12 (17%) in YRI individuals were associated with increased IRF5 expression after Bonferroni correction for multiple testing (table 1).

Increased IRF5 mRNA expression was seen in the CEU sample with multiple SNPs, including SNPs 1, 2, 3, 4, 6, 10, 13 and 14 (table 1). Five of these were tagSNPs, spanning the length of the single 15 kb haplotype block (figure 1B). Similarly, seven SNPs (SNPs 3, 4, 6, 7, 10, 12 and 14; table 1) showed significant association with increased IRF5 expression in CHB+JPT. These SNPs included five tagSNPs that cover both CEU+JPT haplotype blocks (6 kb and 12 kb blocks; figure 1B). In the YRI sample the two SNPs (SNP 10 and 14) that were associated with IRF5 expression tagged block 3 (figure 1B) located in the 3′ gene region; these two SNPs were also associated with increased IRF5 expression in the CEU and CHB+JPT samples (figure 2).

Figure 2
Association of interferon regulatory factor 5 (IRF5) single nucleotide polymorphisms (SNPs) with increased IRF5 expression in three samples. IRF5 expression in European, Chinese/Japanese and Yoruba Nigerian individuals with or without risk alleles (homozygotes ...

Association of IRF5 SNPS, IFNα expression and downstream IFNα pathway activation

SNPs that were significantly associated with IRF5 expression, after correction for multiple testing, were tested for association with total IFNα expression. These results showed two SNPs in the CEU sample (SNP 4, p=0.03; SNP 13, p=0.003) and two SNPs in the CHB+JPT sample (SNP 4, p=0.02; SNP 12, p=0.02) that were associated with increased IFNα expression (p<0.05); however, only SNP 13 (rs10488631) in the CEU sample withstood Bonferroni correction (pc=0.03). Interestingly, SNP 13 is non-polymorphic in the YRI sample; neither of the two SNPs associated with increased IRF5 expression (SNPs 10 and 14) were associated with increased IFNα expression. However, these three SNPs (10, 13 and 14) are located in the 3′ downstream region of the gene and form one haplotype block in the YRI sample.

We next tested only SNPs associated with increased IRF5 and IFNα expression for downstream IFNα pathway activation. Based on this criterion, only SNP 13 in the CEU sample was analysed. This SNP was not tested in the CHB+JPT or the YRI samples owing to unavailability of genotyping (CHB+JPT) or the SNP not being polymorphic (YRI). SNP 13 in the CEU sample was associated with increased IRF5 and IFNα expression and increased CMK score (pC=0.0005, 0.01, 0.04, respectively; figure 3A); IFN scores 1 and 2 showed no significance. A recent report showed that a STAT4 risk allele (rs7574865, T allele) in patients with SLE was dominant over the SLE IRF5 risk allele of rs3807306 with regard to IFN pathway activity.43 We analysed the co-occurrence of the STAT4 and IRF5 risk alleles in our CEU sample but it did not account for our IFN score 1 and 2 results (data not shown).

Figure 3
Mean mRNA expression (±SEM) of interferon regulatory factor 5 (IRF5) and interferon α (IFNα), and mean IFN or CMK scores (±SEM) of (A) individuals of European background (CEU) with or without the systemic lupus erythematosus ...

Haplotypes of IRF5 conferred risk of IFNα pathway activation

Haplotype-based analysis can be much more robust than single marker analysis. A stratified analysis was performed on the CEU haplotypes based on SNP 13 (rs10488631)—that is, individuals with at least one haplotype containing the SNP 13 risk allele were tested against those that did not. Three of the 15 CEU haplotypes (haplotypes 3 (H3), 4 (H4) and 15 (H15); figure 1C) contained the minor C allele of SNP 13. Haplotype 15 was not independently tested as it was found in only one heterozygote individual.

H3 and H4 differed by only the allele at SNP 10, one of two SNPs (the other was SNP 14) associated with increased IRF5 expression in all three ethnic groups (figure 2). We therefore extended our haplotype analysis to include SNPs 10 and 14: H4 contains the three risk alleles of SNPs 10, 13 and 14; H1 contains none of the risk alleles. As shown in figure 3B, the risk haplotype H4 was associated with increased IRF5 and IFNα expression and an increased CMK score compared with the non-risk haplotype H1. There was no significant difference in the two IFN scores using haplotype analysis.

Comparison of results with previous SLE studies

Eleven of the studied SNPs have been associated with SLE (all except SNPs 8, 9 and 11), predominantly in cohorts of mixed European descent.9,11,18,44,45 We did not have genotyping of the four previously reported functional IRF5 SNPs (rs2004640, rs10954213, the exon 6 indel and the CGGGG promoter indel)11,12,16,44 for testing our hypothesis. However, in 379 British families with SLE, SNPs 3 and 14 are in high LD with, respectively, rs2004640, the exon 1B splice site (r2=0.91) and rs10954213, the IRF5 polyadenylation site (r2=0.99).11 The promoter indel and the exon 6 indel were shown to be in relatively high LD with SNPs 3 and 7 (r2=0.7 and 0.61, respectively) in a Swedish sample,45 but these may not fully represent the functional SNPs in CEU samples. For example, although the CGGGG indel was associated with higher IRF5 transcripts in a minigene reporter assay and peripheral blood cells in Swedish patients with SLE,45 SNP 3 was not associated with IFNα pathway activation in this study.

In vitro confirmation of LCL stimulation by IFNα

To confirm the utility of our computer simulated model in vitro, we established EBV-transformed cell lines of seven children with SLE. LCLs were incubated with media alone, recombinant human IFNα and/or the IFNα neutralising agent recombinant B18R protein.42 Expression of IFN-inducible genes MX-1 ISG15, OASL and OAS1, part of IFN score 1, was inducible by IFNα in these LCLs (p=0.0002; figure 4). The addition of an IFN receptor competitive inhibitor, B18R, abolished this increased expression which was not observed with the non-IFN-inducible housekeeping gene, RFLPO.

Figure 4
Increased relative expression of interferon-inducible genes, but not housekeeping genes, was seen in lymphoblastoid cells (LCLs) after interferon α (IFNα) stimulation. Relative expression of genes in LCLs incubated with IFNα was ...

DISCUSSION

Using publicly available databases, we have shown that the major alleles of two correlated IRF5 3′ SNPs, rs13242262 and rs2280714 (SNPs 10 and 14), are associated with increased IRF5 expression in unstimulated LCLs from unrelated individuals from three ethnic groups. Results from recent publications showed increased IRF5 expression in CEU LCLs containing the risk allele of SNP 14,12 and in CEU, CHB and JPT LCLs containing the risk allele of rs10954213,11,14,44 for which SNP 10 is a proxy. In our study, SNPs spanning IRF5 were associated with increased IRF5 transcripts in the CEU and CHB+JPT samples, but only SNPs tagging for the 3′ block (figure 1B) were associated with increased mRNA in the YRI sample. One SNP from the 3′ region (rs10488631) in the CEU sample showed increased IFNα levels by multiple testing criteria; this was not seen in the other populations. IRF5 studies of SLE in East Asians indicate that the 3′ region may be less important to SLE susceptibility than in individuals of European descent.14,46 Whether one region of IRF5 is more important in the regulation of gene expression to certain ethnic groups awaits further testing in larger samples.

We report that the minor allele of rs10488631 (SNP 13) was associated with increased IRF5, total IFNα and IFN-inducible chemokine transcripts in unstimulated CEU LCLs. Along with a CGGGG promoter indel, rs10488631 has accounted for the strongest association signals observed with IRF5 in Swedish SLE studies.45 rs10488631 can be considered a marker for an SLE risk haplotype, as described in two large-scale studies of diverse European ancestries, which contains the three other functional IRF5 risk alleles: rs2004640, rs10954213 and the exon 6 indel.9,44 An IRF5 SLE risk haplotype has been associated with increased IFNα pathway activation in European and Hispanic Americans with SLE;29 the same has not been reported in healthy individuals. In our study, a CEU haplotype defined by the risk alleles of rs13242262, rs10488631 and rs2280714 was associated with increased IRF5, total IFNα and IFN-inducible chemokine transcripts. The CGGGG indel is in LD with the risk allele of rs10488631 (r2=0.61), which has been associated with SLE in European and African American cohorts11,15 and is present in our risk haplotype. This SNP was not associated with increased IRF5 expression in the YRI sample but, as the YRI sample does not reflect the genetic admixture seen in African Americans, this again shows that different IRF5 variations may impact on the IFNα pathway among ethnic groups.

The representation of SNPs across IRF5 in our haplotypes allowed for a straightforward comparison with previously reported SLE risk haplotypes. In 555 families of diverse European descent, SNPs from three groups (rs2004640, the exon 6 indel and rs10954213) are contained in a risk haplotype for SLE.44 Risk alleles of SNPs representing rs2004640 and rs10954213 (as described in the Results section) are present in our CEU risk haplotype. In another report, two over-transmitted haplotypes made up of six SNPs were found by transmission disequilibrium test analysis of UK SLE trios.11 Of these, SNPs 2, 3, 13 and 14 overlapped with our analysis and the alleles present in our CEU risk haplotype were the same as in the SLE study. Finally, a large combined study from eight European countries identified the minor allele of rs10488631 as defining a susceptibility haplotype in European patients with SLE.9 As we reported, the minor allele of this SNP is contained in our CEU risk haplotype. Although IRF5 polymorphisms, including rs2004640, have been associated with SLE in East Asians,14,46,47 the results in these populations do not report identical SLE risk haplotypes as those in individuals of European descent. Specifically, haplotypes containing the three European risk alleles of rs2004640, the exon 6 indel and rs10954213 are not associated with an increased risk of SLE in Japanese and Korean samples.14,46 Our study did not identify an association between IRF5 variants and IFNα pathway activation in CHB+JPT or YRI individuals. It remains to be seen whether other variants not included in this study may be responsible for pathway activation in non-European populations, or whether other genes involved in IFNα regulation may play a more important role.

Given the heterogeneity and incomplete penetrance of SLE, and given the relatively small role that B cells play in the IFN response, it is remarkable to observe an association of IRF5 variants with unstimulated mRNA levels of IFNα and IFN-inducible chemokines, however small, in LCLs from Hapmap individuals. The lack of association of IRF5 variants with IFN-inducible genes in these individuals may be explained by a number of factors. It is plausible that other transcription factors such as IRF7, IRF3, OPN or STAT4 may be involved in activation of the IFNα pathway,48 or that this association may only be present after stimulation by autoantibodies or IFNα. A recent study showed that some patients with SLE had raised levels of IFN-inducible genes and chemokines whereas others only had raised levels of IFN-inducible chemokines,41 suggesting a non-linear pattern of modulation of IFN-inducible genes and downstream chemokines. Based on the results from our computer simulated study, we are investigating whether genetic variants of the IFNα signalling pathway regulate its threshold of activation, potentially contributing to the development of SLE and other autoimmune diseases in unaffected individuals of different populations.

Acknowledgments

Funding for this project was provided by the NIH (National Institute of Arthritis and Musculoskeletal and Skin Diseases) grant R01 AR43814, the NIH (National Institute of Child Health and Human Development) Institutional Research and Academic Career Development Award 5 K12 HC034510, the American College of Rheumatology Research and Education Foundation’s Physician Scientist Development Award, the Arthritis Foundation Southern California Chapter and the Lupus Foundation of America (funding from the Lupus Foundation of America was made possible by the support of the Wallace H Coulter Foundation in memory of Michael Jon Barlin). We would like to thank Francesca Fike and Robert Davies for their assistance with the paediatric SLE lymphoblastoid cell lines.

Footnotes

Ethics approval This study was conducted with the approval of the UCLA Institutional Review Board.

Provenance and peer review Not commissioned; externally peer reviewed.

References

1. Crow MK, Kirou KA. Interferon-alpha in systemic lupus erythematosus. Curr Opin Rheumatol. 2004;16:541–7. [PubMed]
2. Pascual V, Farkas L, Banchereau J. Systemic lupus erythematosus: all roads lead to type I interferons. Curr Opin Immunol. 2006;18:676–82. [PubMed]
3. Niewold TB, Hua J, Lehman TJ, et al. High serum IFN-alpha activity is a heritable risk factor for systemic lupus erythematosus. Genes Immun. 2007;8:492–502. [PMC free article] [PubMed]
4. Pascual V, Banchereau J, Palucka AK. The central role of dendritic cells and interferon-alpha in SLE. Curr Opin Rheumatol. 2003;15:548–56. [PubMed]
5. Rönnblom L, Alm GV. Systemic lupus erythematosus and the type I interferon system. Arthritis Res Ther. 2003;5:68–75. [PubMed]
6. Theofilopoulos AN, Baccala R, Beutler B, et al. Type I interferons (alpha/beta) in immunity and autoimmunity. Annu Rev Immunol. 2005;23:307–36. [PubMed]
7. Abelson AK, Delgado-Vega AM, Kozyrev SV, et al. STAT4 associates with systemic lupus erythematosus through two independent effects that correlate with gene expression and act additively with IRF5 to increase risk. Ann Rheum Dis. 2009;68:1746–53. [PMC free article] [PubMed]
8. D’Alfonso S, Barizzone N, Giordano M, et al. Two single-nucleotide polymorphisms in the 5′ and 3′ ends of the osteopontin gene contribute to susceptibility to systemic lupus erythematosus. Arthritis Rheum. 2005;52:539–47. [PubMed]
9. Ferreiro-Neira I, Calaza M, Alonso-Perez E, et al. Opposed independent effects and epistasis in the complex association of IRF5 to SLE. Genes Immun. 2007;8:429–38. [PubMed]
10. Cunninghame Graham DS, Akil M, Vyse TJ. Association of polymorphisms across the tyrosine kinase gene, TYK2 in UK SLE families. Rheumatology (Oxford) 2007;46:927–30. [PubMed]
11. Cunninghame Graham DS, Manku H, Wagner S, et al. Association of IRF5 in UK SLE families identifies a variant involved in polyadenylation. Hum Mol Genet. 2007;16:579–91. [PMC free article] [PubMed]
12. Graham RR, Kozyrev SV, Baechler EC, et al. A common haplotype of interferon regulatory factor 5 (IRF5) regulates splicing and expression and is associated with increased risk of systemic lupus erythematosus. Nat Genet. 2006;38:550–5. [PubMed]
13. Han S, Guthridge JM, Harley IT, et al. Osteopontin and systemic lupus erythematosus association: a probable gene-gender interaction. PLoS ONE. 2008;3:e0001757. [PMC free article] [PubMed]
14. Kawasaki A, Kyogoku C, Ohashi J, et al. Association of IRF5 polymorphisms with systemic lupus erythematosus in a Japanese population: support for a crucial role of intron 1 polymorphisms. Arthritis Rheum. 2008;58:826–34. [PubMed]
15. Kelly JA, Kelley JM, Kaufman KM, et al. Interferon regulatory factor-5 is genetically associated with systemic lupus erythematosus in African Americans. Genes Immun. 2008;9:187–94. [PubMed]
16. Kozyrev SV, Lewén S, Reddy PM, et al. Structural insertion/deletion variation in IRF5 is associated with a risk haplotype and defines the precise IRF5 isoforms expressed in systemic lupus erythematosus. Arthritis Rheum. 2007;56:1234–41. [PubMed]
17. Palomino-Morales RJ, Rojas-Villarraga A, González CI, et al. STAT4 but not TRAF1/C5 variants influence the risk of developing rheumatoid arthritis and systemic lupus erythematosus in Colombians. Genes Immun. 2008;9:379–82. [PubMed]
18. Sigurdsson S, Nordmark G, Göring HH, et al. Polymorphisms in the tyrosine kinase 2 and interferon regulatory factor 5 genes are associated with systemic lupus erythematosus. Am J Hum Genet. 2005;76:528–37. [PMC free article] [PubMed]
19. Sigurdsson S, Nordmark G, Garnier S, et al. A risk haplotype of STAT4 for systemic lupus erythematosus is over-expressed, correlates with anti-dsDNA and shows additive effects with two risk alleles of IRF5. Hum Mol Genet. 2008;17:2868–76. [PMC free article] [PubMed]
20. Xu AP, Bai J, Lü J, et al. Osteopontin gene polymorphism in association with systemic lupus erythematosus in Chinese patients. Chin Med J. 2007;120:2124–8. [PubMed]
21. Dieguez-Gonzalez R, Calaza M, Perez-Pampin E, et al. Association of interferon regulatory factor 5 haplotypes, similar to that found in systemic lupus erythematosus, in a large subgroup of patients with rheumatoid arthritis. Arthritis Rheum. 2008;58:1264–74. [PubMed]
22. Shimane K, Kochi Y, Yamada R, et al. A single nucleotide polymorphism in the IRF5 promoter region is associated with susceptibility to rheumatoid arthritis in the Japanese population. Ann Rheum Dis. 2009;68:377–83. [PubMed]
23. Sigurdsson S, Padyukov L, Kurreeman FA, et al. Association of a haplotype in the promoter region of the interferon regulatory factor 5 gene with rheumatoid arthritis. Arthritis Rheum. 2007;56:2202–10. [PubMed]
24. Kristjansdottir G, Sandling JK, Bonetti A, et al. Interferon regulatory factor 5 (IRF5) gene variants are associated with multiple sclerosis in three distinct populations. J Med Genet. 2008;45:362–9. [PMC free article] [PubMed]
25. Dideberg V, Kristjansdottir G, Milani L, et al. An insertion-deletion polymorphism in the interferon regulatory Factor 5 (IRF5) gene confers risk of inflammatory bowel diseases. Hum Mol Genet. 2007;16:3008–16. [PubMed]
26. Miceli-Richard C, Comets E, Loiseau P, et al. Association of an IRF5 gene functional polymorphism with Sjögren’s syndrome. Arthritis Rheum. 2007;56:3989–94. [PMC free article] [PubMed]
27. Baechler EC, Bauer JW, Slattery CA, et al. An interferon signature in the peripheral blood of dermatomyositis patients is associated with disease activity. Mol Med. 2007;13:59–68. [PMC free article] [PubMed]
28. Walsh RJ, Kong SW, Yao Y, et al. Type I interferon-inducible gene expression in blood is present and reflects disease activity in dermatomyositis and polymyositis. Arthritis Rheum. 2007;56:3784–92. [PMC free article] [PubMed]
29. Niewold TB, Kelly JA, Flesch MH, et al. Association of the IRF5 risk haplotype with high serum interferon-alpha activity in systemic lupus erythematosus patients. Arthritis Rheum. 2008;58:2481–7. [PMC free article] [PubMed]
30. Niewold TB, Rivera TL, Buyon JP, et al. Serum type I interferon activity is dependent on maternal diagnosis in anti-SSA/Ro-positive mothers of children with neonatal lupus. Arthritis Rheum. 2008;58:541–6. [PMC free article] [PubMed]
31. Baechler EC, Batliwalla FM, Karypis G, et al. Interferon-inducible gene expression signature in peripheral blood cells of patients with severe lupus. Proc Natl Acad Sci USA. 2003;100:2610–15. [PMC free article] [PubMed]
32. Bauer JW, Baechler EC, Petri M, et al. Elevated serum levels of interferon-regulated chemokines are biomarkers for active human systemic lupus erythematosus. PLoS Med. 2006;3:e491. [PMC free article] [PubMed]
33. Bennett L, Palucka AK, Arce E, et al. Interferon and granulopoiesis signatures in systemic lupus erythematosus blood. J Exp Med. 2003;197:711–23. [PMC free article] [PubMed]
34. Feng X, Wu H, Grossman JM, et al. Association of increased interferon-inducible gene expression with disease activity and lupus nephritis in patients with systemic lupus erythematosus. Arthritis Rheum. 2006;54:2951–62. [PubMed]
35. Kirou KA, Lee C, George S, et al. Coordinate overexpression of interferon-alpha-induced genes in systemic lupus erythematosus. Arthritis Rheum. 2004;50:3958–67. [PubMed]
36. Kirou KA, Lee C, George S, et al. Activation of the interferon-alpha pathway identifies a subgroup of systemic lupus erythematosus patients with distinct serologic features and active disease. Arthritis Rheum. 2005;52:1491–503. [PubMed]
37. Santer DM, Yoshio T, Minota S, et al. Potent induction of IFN-alpha and chemokines by autoantibodies in the cerebrospinal fluid of patients with neuropsychiatric lupus. J Immunol. 2009;182:1192–201. [PMC free article] [PubMed]
38. Anon The International HapMap Project. Nature. 2003;426:789–796. [PubMed]
39. Huang RS, Duan S, Shukla SJ, et al. Identification of genetic variants contributing to cisplatin-induced cytotoxicity by use of a genomewide approach. Am J Hum Genet. 2007;81:427–37. [PMC free article] [PubMed]
40. Spielman RS, Bastone LA, Burdick JT, et al. Common genetic variants account for differences in gene expression among ethnic groups. Nat Genet. 2007;39:226–31. [PMC free article] [PubMed]
41. FuQ, Chen X, Cui H, et al. Association of elevated transcript levels of interferon-inducible chemokines with disease activity and organ damage in systemic lupus erythematosus patients. Arthritis Res Ther. 2008;10:R112. [PMC free article] [PubMed]
42. Liptáková H, Kontseková E, Alcamí A, et al. Analysis of an interaction between the soluble vaccinia virus-coded type I interferon (IFN)-receptor and human IFN-alpha1 and IFN-alpha2. Virology. 1997;232:86–90. [PubMed]
43. Kariuki SN, Kirou KA, MacDermott EJ, et al. Cutting edge: autoimmune disease risk variant of STAT4 confers increased sensitivity to IFN-alpha in lupus patients in vivo. J Immunol. 2009;182:34–8. [PMC free article] [PubMed]
44. Graham RR, Kyogoku C, Sigurdsson S, et al. Three functional variants of IFN regulatory factor 5 (IRF5) define risk and protective haplotypes for human lupus. Proc Natl Acad Sci USA. 2007;104:6758–63. [PMC free article] [PubMed]
45. Sigurdsson S, Göring HH, Kristjansdottir G, et al. Comprehensive evaluation of the genetic variants of interferon regulatory factor 5 (IRF5) reveals a novel 5 bp length polymorphism as strong risk factor for systemic lupus erythematosus. Hum Mol Genet. 2008;17:872–81. [PubMed]
46. Shin HD, Kim I, Choi CB, et al. Different genetic effects of interferon regulatory factor 5 (IRF5) polymorphisms on systemic lupus erythematosus in a Korean population. J Rheumatol. 2008;35:2148–51. [PubMed]
47. Siu HO, Yang W, Lau CS, et al. Association of a haplotype of IRF5 gene with systemic lupus erythematosus in Chinese. J Rheumatol. 2008;35:360–2. [PubMed]
48. Fitzgerald-Bocarsly P, Dai J, Singh S. Plasmacytoid dendritic cells and type I IFN: 50 years of convergent history. Cytokine Growth Factor Rev. 2008;19:3–19. [PMC free article] [PubMed]
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