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BMC Genet. 2001; 2: 5.
Published online Mar 19, 2001. doi:  10.1186/1471-2156-2-5
PMCID: PMC31344

NRG1 is required for glucose repression of the SUC2 and GAL genes of Saccharomyces cerevisiae

Abstract

Background

Glucose repression of transcription in the yeast, Saccharomyces cerevisiae, has been shown to be controlled by several factors, including two repressors called Mig1 and Mig2. Past results suggest that other repressors may be involved in glucose repression.

Results

By a screen for factors that control transcription of the glucose-repressible SUC2 gene of S. cerevisiae, the NRG1 gene was identified. Analysis of an nrg1Δ mutant has demonstrated that mRNA levels are elevated at both the SUC2 and of the GAL genes of S. cerevisiae when cells are grown under normally glucose-repressing conditions. In addition, genetic interactions have been detected between nrg1Δ and other factors that control SUC2 transcription.

Conclusions

The analysis of nrg1Δ demonstrates that Nrg1 plays a role in glucose repression of the SUC2 and GAL genes of S. cerevisiae. Thus, three repressors, Nrg1, Mig1, and Mig2, are involved as the downstream targets of the glucose signaling in S. cerevisiae.

Background

For the yeast Saccharomyces cerevisiae, glucose is the preferred carbon source. When glucose is present in the growth media, transcription of a large number of genes encoding products involved in the metabolism of alternative carbon sources is repressed (for reviews, see [1,2,3]. These genes include the GAL, SUC2, MAL and STA genes, required, respectively, for the utilization of galactose, sucrose/raffinose, maltose, and starch.

At many of these genes, glucose repression is mediated, at least in part, by the glucose-dependent repressor Mig1, a zinc-finger protein that binds in vitro to DNA consensus sites consisting of a GC-rich core and flanking AT sequences [4, 5]. Mig1 is thought to bind to several promoters, including GAL1, GAL4, SUC2 and MAL62, and to effect transcriptional repression by interacting with the co-repressor complex Ssn6-Tup1 [6,7,8]. Mig1's activity is regulated by phosphorylation and subcellular localization: in high glucose, Mig1 protein is hypophosphorylated and in the nucleus, where it can repress transcription; upon withdrawal of glucose, Mig1 is rapidly phosphorylated and transported into the cytoplasm [9]. This regulated phosphorylation requires the function of the Snf1/Snf4 kinase complex [10].

Deletion of MIG1, however, only partially relieves glucose repression at promoters such as SUC2, whereas deletion of either SSN6 or TUP1 completely abolishes glucose repression. Moreover, the STA1 gene of S. cerevisiae var. diastaticus, which is also repressed by glucose, is unaffected by mig1Δ [11]. Therefore, other proteins in addition to Mig1 are required for glucose repression. One of these proteins is Mig2, which shares sequence similarity with Mig1 in their zinc finger regions [12, 13]. Genetic analysis suggests that Mig2 plays a minor role relative to Mig1.

Recently, a previously uncharacterized gene, NRG1 (Negative regulator of glucose-repressed genes), was shown to be required for glucose repression of the STA1 gene in S. cerevisiae var. diastaticus [11]. These studies demonstrated that LexA-Nrg1 behaves as a repressor of a reporter construct and that this repression is dependent on glucose, Ssn6, and Tup1. In addition, Nrg1 and Ssn6 interact with each other in two-hybrid and GST pull-down assays, indicating that Nrg1 may repress via the same pathway as Mig1. Consistent with these results, Nrg1 appears to bind to two sites within the STA1 promoter.

The SUC2 gene of S. cerevisiae has been extensively studied with respect to its glucose repression [1,2]. Glucose repression of SUC2 is mediated by Ssn6/Tup1 and SUC2 has two Mig1 binding sites in its regulatory region. Additionally, in high glucose its promoter is also occupied by positioned nucleosomes, which cause transcriptional repression themselves [14, 15]. Derepression in low glucose is correlated with a loss of both Mig1- and nucleosome-mediated repression, although the precise relationship between the two pathways is not clear.

Genetic screens have identified a large number of genes, named SNF (Sucrose Non-Fermenting) that are required for derepression of SUC2 transcription in the absence of glucose [16,17,18]. Genetic analyses and subsequent studies have traditionally divided SNF genes into two groups. One group encodes the protein kinase Snf1 and its associated regulator Snf4, required to antagonize the repression caused by Mig1 [10, 19]. The other group consists of members of the Swi/Snf complex required to counter the repressive effects of chromatin by remodeling nucleosomes in an ATP-dependent manner (for review see [20]. Suppressors of swi/snf mutations, such as spt6, do not suppress snf1Δ [21], and ssn6, a strong suppressor of snf1Δ, only partially suppress swi/snf mutations [22].

In this work, we report the identification of Nrg1 in a genetic screen for new regulators of SUC2 transcription. We show that Nrg1 plays a role in the glucose repression of SUC2 and GAL genes in S. cerevisiae. Thus, at these genes, Mig1, Mig2 and Nrg1 are partially redundant for mediating repression by glucose. Consistent with our findings, recent results have demonstrated an interaction between Snf1 and Nrg1 [23]. We also present experiments that test the genetic interactions between mig1Δ, nrg1Δ and deletions of various genes encoding activators that function at the SUC2 promoter.

Results

Isolation of a high-copy-number suppressor of snf2Δ

The Swi/Snf complex is required for normal levels of expression of SUC2 when cells are grown in low glucose. To identify factors that might be functionally related to Swi/Snf, we screened for high-copy-number plasmids that could suppress a snf2Δ mutation (see Materials and Methods). To sensitize the screen, we used an allele of SUC2, SUC2-36, that allows an elevated level of SUC2 transcription in the absence of Swi/Snf [24]. The SUC2-36 mutation is a single base pair change, AT to GC at position -401 relative to the SUC2 ATG. SUC2-36 strains still have a Raf- phenotype in a snf2Δ mutant.

To identify high-copy-number suppressor candidates, we used a 2μ circle library to transform the snf2Δ SUC2-36 strain FY1845 (Table (Table1)1) and screened 60,000 transformants for those with a Raf+ phenotype. Eighty-two candidates were identified, 25 of which contained the SNF2 gene. Among the remaining plasmids, most conferred a weak Raf+ phenotype. We focused on the candidate that conferred the strongest Raf+ phenotype. This plasmid contained a chromosome IV genomic fragment that spans from within the NRG1 gene (open reading frame YDR043C) through the HEM12 gene (YDR047W). Subcloning experiments identified the partial NRG1 clone as the sequence responsible for suppression of snf2Δ and demonstrated that this suppression occurred in both SUC2-36 and SUC2+ genetic backgrounds (Figure (Figure11).

Figure 1
Overexpressing a truncated clone of NRG1 suppresses snf2Δ. Yeast strains FY32 (snf2Δ1::HIS3 SUC2) and yHZ269 (snf2Δ1::HIS3 SUC2-36) were transformed with nrglΔZn or full-length NRG1 cloned in pRS426, as well as vector alone. ...
Table 1
Yeast Strains

NRG1 is predicted to encode a protein of 231 amino acids with two C2H2 zinc fingers in the carboxyl terminus. Sequence analysis revealed that the 2μ plasmid that confers suppression of snf2Δ encodes just the amino terminal region of Nrg1, lacking the zinc fingers. To test if the complete NRG1 gene causes the same high copy number phenotype, we subcloned the complete NRG1 gene into a 2μ plasmid and tested it for suppression of snf2Δ. Our results demonstrate that the complete NRG1 gene on a 2μ plasmid does not suppress snf2Δ (Figure (Figure11).

NRG1 encodes a repressor of transcription

To characterize further the role of Nrg1 with respect to SUC2 transcription, we constructed and analyzed an nrg1Δ mutant. The nrg1Δ mutant grows normally on media containing glucose, sucrose, or galactose, demonstrating that NRG1 is not essential for grwoth and that nrg1Δ mutants can utilize several different carbon sources.

To test for the requirement for Nrg1 in glucose repression, we tested growth of an nrg1Δ mutant on YP sucrose media containing the glucose analog, 2-deoxyglucose (2-DG). 2-DG causes glucose repression but cannot be used as a carbon source by S. cerevisiae. Therefore, wild-type cells do not grow on YP sucrose plates that contain 2-DG, due to glucose repression of SUC2. However, strains defective for glucose repression can grow on this medium as they express SUC2 even in the presence of 2-DG. We found that an nrg1Δ mutant was able to grow on YP sucrose plus 2-DG, suggesting that nrg1Δ mutants are indeed defective for glucose repression. To assess the role of Nrg1 relative to the two other factors known to be required for glucose repression, Mig1 and Mig2, we compared the mutant phenotypes caused by nrg1Δ, mig1Δ, and mig2Δ, as well as testing combinations of these deletions. We observed that the three single mutants grow with different strengths on YP sucrose 2-DG plates in the order mig1Δ > nrg1Δ > mig2Δ (Figure (Figure2).2). The double and triple mutants had stronger phenotypes than the single mutants (Figure (Figure2).2). These results strongly suggest that Nrg1, Mig1, and Mig2 are each required for glucose repression at the SUC2 locus, with Mig1 playing the major role. We also tested growth of these strains on YP galactose + 2-DG plates and found that only the triple mutant was able to grow, albeit weakly, on this medium, perhaps because galactose is a poor carbon source (data not shown). This suggests that each of these three proteins contributed to glucose repression of the GAL genes.

Figure 2
Deletion of NRG1 partially abolishes glucose repression. nrg1Δ allows cells to grow on sucrose plates containing 2-deoxyglucose, and has additive effects with mig1Δ and mig2Δ. A single colony of each strain was inoculated ...

Glucose repression of transcription is defective in nrg1Δ

To test whether the nrg1Δ phenotype on 2-DG plates is caused by altered transcription, we performed Northern analyses to SUC2 mRNA levels. Under repressing conditions (2% glucose), the level of SUC2 mRNA was increased by two-to-four fold in an nrg1Δ strain compared to a wild-type control (Figure (Figure3A).3A). Consistent with previously published results, a mig1Δ mutant had a nine- to-fourteen fold increase in SUC2 mRNA levels while a mig2Δ mutant had no detectable defect in glucose repression of SUC2 [4, 12]. We also analyzed the SUC2 mRNA levels in double and triple mutant combinations. In general, multiple mutations caused greater derepression, up to 79-fold for the triple mutant, nrg1Δ mig1Δ mig2Δ (Figure (Figure3A).3A). These data demonstrate that Nrg1, Mig1, and Mig2 all contribute to the glucose repression of SUC2.

Figure 3
Deletion of NRG1 causes defects in glucose repression. (A) A single colony of each strain was inoculated into YPD liquid with 2% glucose and grown to mid-log phase (approx. 1 × 107 cells/ml). The cells were harvested, and total RNA was isolated ...

We also tested if an nrg1Δ affects glucose repression of the GAL genes as described in Materials and Methods. Both nrg1Δ and mig1Δ mutations cause a defect in the glucose repression of GAL1 and GAL10, whereas mig2Δ alone had no effect (Figure (Figure3B).3B). As for SUC2, additive effects were observed in double and triple mutant strains, up to a 13-fold effect for the nrg1Δ mig1Δ mig2Δ triple mutant (Figure (Figure3B).3B). These data indicate that all three proteins are involved in glucose repression of GAL1-GAL10, with Mig2 playing only a minor role.

Deletion of MIG1 or NRG1 suppresses mutations in both SNF1 and SWI/SNF genes

Activation of SUC2 transcription depends upon both the Snf1/Snf4 kinase complex and the Swi/Snf nucleosome remodeling complex. To address the relationship of Nrg1 to both complexes and to compare it to Mig1, we tested the abilities of nrg1Δ and mig1Δ to suppress the Gal-, Suc-, and Raf- phenotypes of mutations in SNF1 and SWI/SNF genes.

Our results (Figure (Figure4)4) show that both nrg1Δ and mig1Δ suppress, albeit sometimes weakly, mutations in both SNF1 and SWI/SNF genes. With respect to suppression of snf1Δ, mig1Δ is the stronger suppressor, with suppression detectable for the Gal" phenotype (Figure (Figure4A).4A). The observed suppression by mig1Δ is consistent with previous results [22]. The nrg1Δ mutation did not detectably suppress either the Suc- or Raf- phenotypes caused by snf1Δ. With respect to swi/snf mutations, we tested suppression of both snf2Δ and swp73Δ and observed weak suppression of the Gal- and Suc phenotypes (Figure (Figure4B).4B). Suppression of the Raf- phenotype was not detectable. There appear to be some gene-specific interactions as suppression of swp73Δ by mig1Δ was stronger than the suppression observed for the other pairs tested.

Figure 4
Mutations in SNF1 and SNF/SWI can be suppressed by both nrg1Δ and miglΔ. A single colony of each strain was inoculated into YPD liquid and grown over-night to saturation and adjusted in water to 1 × 108 cells/ml. The cultures were ...

Discussion

Our results demonstrate that Nrg1 plays a role in glucose repression of the SUC2 and GAL genes of S. cerevisiae. Consistent with a role in glucose repression, an nrg1Δ mutation suppresses the defects of a snf1Δ mutant. Recent results from an independent study have demonstrated an interaction between Snf1 and Nrg1 [23]. Our results also suggest that Nrg1 is partially redundant with two other factors required for glucose repression, Mig1 and Mig2. At SUC2 and GAL1-10, all three proteins appear to be involved in glucose repression, because double- and triple-deletion mutations have additive effects. Interestingly, both nrg1Δ and mig1Δ can also suppress the defects caused by mutations in genes encoding members of the Swi/Snf complex.

While Nrg1, Mig1, and Mig2 are partially redundant, current evidence suggestions that they do not function in the same relative fashion at all glucose-repressible promoters. For example, while mig1Δ and nrg1Δ cause comparable defects at GAL1-GAL10, nrg1Δ causes a weaker defect at SUC2. Mig2 appears to have only a minimal function at either promoter. In addition, Nrg1 is the major repressor at STA1, whose glucose-repression does not require Mig1 [11]. Therefore, some gene-specific specialization exists among these three glucose-dependent repressors.

A previous study of Nrg1 provided evidence that it interacts with Ssn6 and confers repression by recruitment of Ssn6/Tup1 [11]. We initially identified NRG1 in our studies by the isolation of a high-copy-number plasmid encoding a fragment of Nrg1, lacking the zinc-finger domain. Likely, the phenotype caused by this plasmid is caused by interference of repression by Ssn6/Tup1.

Our studies have not yet distinguished between a direct or indirect effect of Nrg1 on glucose repression at SUC2 and GAL1-GAL10. One possible indirect effect of Nrg1 could be by regulation of MIG1 transcription. However, Northern analysis showed that MIG1 mRNA levels are unaffected by an nrg1Δ mutation (H. Zhou and F. Winston, unpublished data). We tested Nrg1 for binding to the SUC2 promoter and those experiments are briefly summarized here. We screened for DNA binding of Nrg1 to the SUC2 promoter region using a previously described GST-Nrg1 fusion protein [11] and a gel shift assay. Our results demonstrated specific DNA binding to two sites within the -1022 to -825 region 5' of SUC2 (H. Zhou and F. Winston, unpublished results). However, a deletion of this region does not alter SUC2 expression. Based on the similarity between the zinc fingers of Nrg1 and Mig1 and our binding studies, the binding site of Nrg1 may contain a GC-rich core. Another such site in the SUC2 promoter may occur at -570 with the sequence AGGCCCA. Although we did not detect a gel shift of a fragment containing this site, it is still possible that it is recognized and bound by Nrg1 in vivo. Furthermore, although an Nrg1 consensus binding [11] exists at -976 of SUC2, we were unable to detect binding to this site by GST-Nrg1. This region also did not compete the binding that we detected by GST-Nrg1. This discrepancy between our findings and previous results can be explained by the fact that Park et al [11] used 10-fold more GST-Nrg1 in their binding studies than we did. Finally, we did not detect any binding of Nrg1 to the Mig1 binding sites. Thus, the DNA binding of Nrg1 to SUC2 remains to be resolved.

Conclusions

In conclusion, these studies have identified Nrg1 as a third repressor required for glucose repression at SUC2 and the GAL genes. Based on the similarity between the zinc fingers of Nrg1 and Mig1, the phenotypes of nrg1Δ and mig1Δ, and the reported interaction between Nrg1 and Ssn6 [11], Nrg1 likely functions by binding to the target promoters and recruiting the Ssn6/Tup1 complex. The relative and possible cooperative roles of each of these repressors in recruiting Ssn6-Tup1 remains to be determined.

Materials and methods

Yeast strains

All S. cerevisiae strains are listed in Table Table11 and are in the S288C genetic background [25, 26]. Deletion of MIG1 was achieved by transforming strain yHZ416 with the HindIII digest of pJN22 (for migl-Δ2::LEU2) or pJN41 (for mig1-Δ2::URA3) [4], and selecting for Leu+ or Ura+ transformants, respectively. PCR-directed gene replacement [27] was used to construct deletions of NRG1 and MIG2. PCR reactions were carried out using as templates pRS vectors carrying the desired markers [25, 28]. For NRG1, the oligos used were HZ034, 5' TCG ACC AGC ATA TTA CTA CCC TTC GCA AAC TTT CAG GCA CTG TGC GGT ATT TCA CAC CG 3'; and HZ035, 5' GTA GTA CTG CTA ATG AGA AAA ACA CGG GTA TAC CGT CAA AGA TTG TAC TGA GAG TGC AC 3'. For MIG2, the oligos were HZ045, 5' TGA CCT CGA GAA CAA ACA AAA TAA AAA TAA AAA AAG AGA CTG TGC GGT ATT TCA CAC CG 3'; and HZ046, 5' TTA GAG GAA AAA TGG TGA GAT AAA AAG GGG CCG TAA AGG AGA TTG TAC TGA GAG TGC AC 3'. The PCR fragment was used to transform a haploid strain directly. All gene replacements were verified by PCR, Southern analyses, and tetrad analyses.

Media

The media used in this study were previously described [29]. Glucose, galactose, sucrose or raffinose was added to 2% final weight per volume. For solid media containing a carbon source other than glucose or glycerol, antimycin A was also added to a concentration of 1 μg/ml. To test for glucose repression of SUC2 and GAL genes, 2-deoxyglucose was added to YP sucrose-antimycin A and YP galactose-antimycin A plates to a final concentration of 200 μg/ml [4]. We discovered during the course of this study that a ura3Δ0 strain had half the amount of GAL 1-10 mRNA of a URA3 strain when grown in SD media containing 2% glucose and 2% galactose. A ura3Δ0 strain also grew more slowly than a URA3 strain on minimal media containing sucrose or galactose. We do not yet have an explanation for this phenomenon. To overcome this growth defect, uracil was added to YP plates to a final concentration of 80 μM.

Subcloning of NRG1 constructs

The 1.8 kb SacI-SalI fragment of the original library clone, containing only the 5' half of NrG1 without the zinc fingers, was cloned into the SacI-SalI sites of pRS426 to create pHZ56. To clone the complete NRG1 ORF, HZ032 and HZ033 were used to PCR from genomic DNA the complete wild-type NRG1 from -1119 to +719. The PCR fragment was digested with Sad and cloned into the SacI-SmaI sites of pRS426 to generate pHZ52.

Northern analysis

Cell cultures were grown in liquid media as indicated to mid-log phase (1-2 × l07 cells/ml), and total RNA was prepared as previously described [27,30]. RNA was separated by electrophoresis on 1% agarose-formaldehyde gels, transferred to membrane and blotted with specific radio-labeled probes. The probes were: for SUC2, the 1.3 kb BamHI-HindIII fragment of pRB59 [31]; for GAL1-10, the 2 kb EcoRI-EcoRI fragment of BNN45 [32] and for SPT15, the 0.8 kb SpeI-HindIII fragment of pIP45 (I. Pinto, personal communication). All probes were labeled by random priming.

Acknowledgments

This work was supported by NIH grant GM32967.

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