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Infect Immun. Jun 1989; 57(6): 1834–1838.
PMCID: PMC313363

Role of adherence in pathogenesis of Enterococcus faecalis urinary tract infection and endocarditis.

Abstract

Enterococcus faecalis strains isolated from urinary tract infections (UTIs) and endocarditis were analyzed for their ability to adhere to urinary tract epithelial cells (ECs) and Girardi heart (GH) and human embryonic kidney (HEK) cell cultures. UTI isolates adhered to urinary tract ECs more efficiently than to the cultured cells, at the same time showing the least affinity for GH cells. In contrast, endocarditis isolates adhered to GH cell cultures more readily than to urinary tract ECs. Moreover, although strains isolated from endocarditis adhered to GH cells more efficiently than those derived from UTI, the latter strains adhered to urinary tract cells better than the former. Studies of the ability of GH and HEK cells to internalize E. faecalis showed that for UTI isolates, 9 to 74% of adhered bacteria were internalized, while for endocarditis isolates, the percentage varied from 76 to 82%. All strains were able to associate with human neutrophils; endocarditis strains, however, associated less efficiently than UTI isolates. Growth in serum raised the adherence of all tested strains by at least 1.5- to 3-fold, with the greatest increase being observed in UTI strain adherence to GH cells (8-fold). In contrast, the association of serum-grown cells with polymorphonuclear leukocytes was reduced by two- to fivefold. In both cases, the observed serum-dependent alterations were cancelled by a few subcultures in brain heart infusion broth. These results indicate that adhesive properties are important virulence factors in the pathogenesis of UTI and endocarditis and also suggest that UTI strains showing the highest invasion and adhesive potential invade the kidneys, cause bacteremia, and, after having expressed the serum-dependent surface modification, colonize the heart.

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Selected References

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  • Aitchison EJ, Lambert PA, Farrell ID. Antigenic composition of an endocarditis-associated isolate of Streptococcus faecalis and identification of its glycoprotein antigens by ligand blotting with lectins. J Med Microbiol. 1986 Mar;21(2):161–167. [PubMed]
  • Beachey EH. Bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surface. J Infect Dis. 1981 Mar;143(3):325–345. [PubMed]
  • Bøyum A. Isolation of lymphocytes, granulocytes and macrophages. Scand J Immunol. 1976 Jun;Suppl 5:9–15. [PubMed]
  • Brown MR, Williams P. The influence of environment on envelope properties affecting survival of bacteria in infections. Annu Rev Microbiol. 1985;39:527–556. [PubMed]
  • Chabanon G, Hartley CL, Richmond MH. In vitro attachment to a human cell line of Escherichia coli strains causing urinary-tract infection: occurrence of fimbriae (pili) in adhesive and non-adhesive strains. Ann Microbiol (Paris) 1982 May-Jun;133(3):357–369. [PubMed]
  • Dalhoff A. Differences between bacteria grown in vitro and in vivo. J Antimicrob Chemother. 1985 Jan;15 (Suppl A):175–195. [PubMed]
  • Gould K, Ramirez-Ronda CH, Holmes RK, Sanford JP. Adherence of bacteria to heart valves in vitro. J Clin Invest. 1975 Dec;56(6):1364–1370. [PMC free article] [PubMed]
  • Jones GW, Richardson LA, Uhlman D. The invasion of HeLa cells by Salmonella typhimurium: reversible and irreversible bacterial attachment and the role of bacterial motility. J Gen Microbiol. 1981 Dec;127(2):351–360. [PubMed]
  • KOENIG MG, KAYE D. Enterococcal endocarditis. Report of nineteen cases with long-term follow-up data. N Engl J Med. 1961 Feb 9;264:257–264. [PubMed]
  • Moellering RC, Jr, Watson BK, Kunz LJ. Endocarditis due to group D streptococci. Comparison of disease caused by streptococcus bovis with that produced by the enterococci. Am J Med. 1974 Aug;57(2):239–250. [PubMed]
  • Ofek I, Mirelman D, Sharon N. Adherence of Escherichia coli to human mucosal cells mediated by mannose receptors. Nature. 1977 Feb 17;265(5595):623–625. [PubMed]
  • Rodriguez-Ortega M, Ofek I, Sharon N. Membrane glycoproteins of human polymorphonuclear leukocytes that act as receptors for mannose-specific Escherichia coli. Infect Immun. 1987 Apr;55(4):968–973. [PMC free article] [PubMed]
  • Silverblatt FJ, Dreyer JS, Schauer S. Effect of pili on susceptibility of Escherichia coli to phagocytosis. Infect Immun. 1979 Apr;24(1):218–223. [PMC free article] [PubMed]
  • Sipes JN, Thompson RL, Hook EW. Prophylaxis of infective endocarditis: a reevaluation. Annu Rev Med. 1977;28:371–391. [PubMed]
  • Edén CS, Hanson LA, Jodal U, Lindberg U, Akerlund AS. Variable adherence to normal human urinary-tract epithelial cells of Escherichia coli strains associated with various forms of urinary-tract infection. Lancet. 1976 Sep 4;1(7984):490–492. [PubMed]
  • Väisänen-Rhen V, Elo J, Väisänen E, Siitonen A, Orskov I, Orskov F, Svenson SB, Mäkelä PH, Korhonen TK. P-fimbriated clones among uropathogenic Escherichia coli strains. Infect Immun. 1984 Jan;43(1):149–155. [PMC free article] [PubMed]

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