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Nucleic Acids Res. 2011 Jul 1; 39(Web Server issue): W184–W189.
Published online 2011 Jun 10. doi:  10.1093/nar/gkr430
PMCID: PMC3125794

AquaSAXS: a web server for computation and fitting of SAXS profiles with non-uniformally hydrated atomic models


Small Angle X-ray Scattering (SAXS) techniques are becoming more and more useful for structural biologists and biochemists, thanks to better access to dedicated synchrotron beamlines, better detectors and the relative easiness of sample preparation. The ability to compute the theoretical SAXS profile of a given structural model, and to compare this profile with the measured scattering intensity, yields crucial structural informations about the macromolecule under study and/or its complexes in solution. An important contribution to the profile, besides the macromolecule itself and its solvent-excluded volume, is the excess density due to the hydration layer. AquaSAXS takes advantage of recently developed methods, such as AquaSol, that give the equilibrium solvent density map around macromolecules, to compute an accurate SAXS/WAXS profile of a given structure and to compare it to the experimental one. Here, we describe the interface architecture and capabilities of the AquaSAXS web server (http://lorentz.dynstr.pasteur.fr/aquasaxs.php).


Small Angle X-ray Scattering (SAXS) is a technique that allows the study of the structure and interactions of biological molecules in solution. It can be used to probe proteins, nucleic acids, and their complexes under a variety of conditions, from near physiological to highly denaturing, without the need to crystallize the sample and without the molecular weight limitations inherent in other methods such as NMR spectroscopy.

The increasing availability of high-flux, third-generation synchrotron sources, improvements in detector technology and algorithmic developments for data analysis have made SAXS a technique of choice for a range of biological applications (1).

The basic principle of SAXS is to scatter X-ray photons elastically off molecules in solution, and to record the scattering intensity as a function of the scattering angle. The intensity profile of the buffer is subtracted from the profile of the macromolecule in the buffer, yielding an excess intensity profile, related to the excess electronic density of the molecule and its environment.

The SAXS profile provides information about the global structure and conformation of the studied molecule(s). Several reviews on the physical principles and theory of SAXS describe in detail how the scattering data can be analyzed and how different parameters can be fit and interpreted (2–6). Recent developments and novel applications of SAXS are described in (7–9).

Existing computational approaches for modeling a macromolecular structure based on its SAXS profile can be separated into two classes: profile-to-model (ab initio methods) and model-to-profile approaches. The former aims at proposing coarse shapes represented by dummy beads that fit the experimental profile (10–16), while the latter aims at comparing the theoretical profile of a given atomic or coarse grained model to the experimental one (17,18).

The model-to-profile approach consists in computing the theoretical profile of a given atomic structure and providing a measure of the goodness-of-fit to the experimental profile. Here, we describe a web server (AquaSAXS) that performs this task. It is useful for many applications where one needs to decide whether the proposed model is in agreement with the experiment, and to make assumptions about why they differ, if they do.

Several tools have been designed for that purpose, following various methods (19–24). To our knowledge, the most accurate method to date for computing SAXS profiles of a given macromolecule has been achieved by treating the solvent (excluded and hydrating) explicitly (24). Through this method, even higher resolution profiles resulting from Wide Angle X-Ray Scattering (WAXS) experiments were also reasonably reproduced. However, this approach requires hours of computation to retrieve one profile. All other methods rely on a continuum representation of the solvent-excluded volume, first proposed by Fraser et al. (25): the effective-atomic-scattering-form-factor method. The programs SASSIM (22) and Fast-SAXS (23) rely on configurational averaging over Molecular Dynamics simulations to treat the excess in electron density of the hydration layer relative to bulk solvent. The widely used program CRYSOL (19) makes the assumption that the hydration shell surrounding the solute is a constant density layer of given thickness, while FoXS (20) attributes an additional term to every effective-atomic-scattering-form-factor proportional to their solvent accessible area to account for the hydration layer's contribution.

AquaSAXS aims at providing a new way to treat the solvation layer accurately without the need for extensive Molecular Dynamics simulations. To achieve this goal, AquaSAXS takes advantage of recently developed methods that compute the solvent-distribution around a given solute on a 3D grid such as the Poisson–Boltzmann–Langevin (PBL) formalism (26); PBL is implemented in AquaSol that is freely available online as a web server (27) or as a software available upon request from the authors (28). Here it will be run by default. In principle, AquaSAXS can also take advantage of other methods that compute the solvent-distribution surrounding a macromolecule. For example, outputs from 3D-RISM (Three Dimensional-Reference Interaction Site Model) calculations can readily be used by AquaSAXS. The 3D-RISM theory has been implemented in the latest AmberTools package (29).

In addition, AquaSAXS provides a way for the user to check and/or tune the atomic types and the corresponding parameters that are used for computing the solute and the solvent-excluded-volume contribution to the SAXS profile. In the following sections, we describe the method and interface of AquaSAXS.


In the infinite dilution limit, assuming that the macromolecule has one preferential conformation, the scattering intensity of a sample solution containing the macromolecule is proportional to the scattering intensity of a single macromolecule of scattering density An external file that holds a picture, illustration, etc.
Object name is gkr430i1.jpg (r), surrounded by a solvent of average electron density An external file that holds a picture, illustration, etc.
Object name is gkr430i2.jpg. The scattering intensity An external file that holds a picture, illustration, etc.
Object name is gkr430i3.jpg of this macromolecule at a given value of the wavevector norm q is the spherical average of the scattering intensity on the sphere of radius q in reciprocal space (Equation 1).

equation image

where A is the excess form factor of the system and An external file that holds a picture, illustration, etc.
Object name is gkr430i4.jpg are respectively the form factor of the solute in vacuo and of the solvent-excluded-volume. An external file that holds a picture, illustration, etc.
Object name is gkr430i5.jpg is the excess form factor of the hydration shell.

We perform the spherical averaging using the cubature formulae (30). Following the effective-atomic-scattering-form-factor method (25), the solute and the solvent-excluded-volume's form factors are computed as a sum over all N non-Hydrogen atoms of the solute (Equations 2 and 3).

equation image

equation image

where fj is the atomic form factor in vacuo (19), computed as a sum of a constant and four gaussians whose parameters depend on the atom type, An external file that holds a picture, illustration, etc.
Object name is gkr430i6.jpg is the solvent volume displaced by atom j. An external file that holds a picture, illustration, etc.
Object name is gkr430i7.jpg is an overall expansion factor, as defined in (19), with C1 being the ratio of the adjusted and computed average atomic radii (default value = 1.0).

Programs such as AquaSol (28) [or 3D-RISM/Amber (29)] compute solvent density maps around the solute, based on the physical interactions within the system. The method used in AquaSol is based on the Poisson–Boltzmann formalism, where the solvent is no longer described as a continuum dielectric medium but rather as an assembly of self-orienting dipoles of variable density on a grid. It was shown that the resulting water distribution is in good agreement with experimental data and with the chemical nature of the atoms exposed to the solvent, both at the atomic and residue-level (26). These maps are typically cubic grids of given size and resolution (a), where each grid point r is associated to a given density value An external file that holds a picture, illustration, etc.
Object name is gkr430i8.jpg. Basically, in such maps, one expects a density of 0 inside the solute, and 1 (in units of bulk density An external file that holds a picture, illustration, etc.
Object name is gkr430i9.jpg) in the bulk region of the solvent, i.e. far from the solute. At the boundary between the solute and bulk region, the density is determined by the physico–chemical nature of the environment.

We compute the form factor for the hydration shell as in Equation 4.

equation image

The sum runs over all points with nonzero density. In practice, to reduce computation time, grid points with a density close to 1 (i.e. typically within An external file that holds a picture, illustration, etc.
Object name is gkr430i10.jpg1.10−4) are removed from the sum. On Urate Oxidase (example mentioned below), allowing a tolerance of 1.10−6 slowed down the computation by a factor of three and did not affect the resulting profile: the same fitting parameters were found, and the goodness-of-fit An external file that holds a picture, illustration, etc.
Object name is gkr430i11.jpg (cf Equation 6) was similar (1.688 versus 1.691).

Besides the solute and solvent, another possible contributor to the SAXS profile is the ion atmosphere surrounding the solute. AquaSol (28) computes the density maps of free cations and anions, and, in principle, these maps could be used to compute the excess form factors of ions. However, at physiological concentrations (200 mM NaCl) the ratio of the fugacities of ions and water is < 0.5%. At this stage, the contribution of ions was not implemented into AquaSAXS, except in the form of explicitly bound and fixed ions. Nevertheless, the presence of free ions can indirectly affect the solvent density in the hydration shell (screening effect), so the user is prompted for the ionic strength of the solution.

Alternatively, the hydration shell's form factor can be computed as in FoXS (20), following Equation 5.

equation image

where An external file that holds a picture, illustration, etc.
Object name is gkr430i13.jpg is the fraction of solvent accessible surface of the atom j (31) and An external file that holds a picture, illustration, etc.
Object name is gkr430i14.jpg is the water form factor. An external file that holds a picture, illustration, etc.
Object name is gkr430i15.jpg is a scale factor used to adjust the hydration shell's contribution (default value = 1.0).

The computed profile An external file that holds a picture, illustration, etc.
Object name is gkr430i16.jpg is fitted to a given experimental SAXS profile An external file that holds a picture, illustration, etc.
Object name is gkr430i17.jpg (with experimental error An external file that holds a picture, illustration, etc.
Object name is gkr430i18.jpg) by minimizing the goodness-of-fit function An external file that holds a picture, illustration, etc.
Object name is gkr430i19.jpg with respect to three adjustable parameters: C1, C2 and C (Equation 6).

equation image

C1 and C2 values are scanned within a given range (An external file that holds a picture, illustration, etc.
Object name is gkr430i20.jpg, An external file that holds a picture, illustration, etc.
Object name is gkr430i21.jpg and An external file that holds a picture, illustration, etc.
Object name is gkr430i22.jpg), in steps of 0.0055, 0.014 and 0.04, resp., and for each pair, a linear-least-squares minimization is performed to adjust the scaling constant C. The pair leading to the minimal χ is kept to compute the returned profile.


AquaSAXS was successfully tested with all PDB (32) structures that have an experimental SAXS profile in the open access BioIsis database (33), and gave results similar to CRYSOL and FoXS. It was also tested on Urate Oxidase (see Figure 2).

Figure 2.
Typical result of AquaSAXS on Urate Oxidase. The deposited structure 3L8W was used as model. The PQR file was generated with PDB2PQR (34), using CHARMM parameters. The solvent map was generated with AquaSol. 65 points per edge, equally spaced by 2.2 Å ...

The calculation scales linearly with the number of points at which the profile is sampled (An external file that holds a picture, illustration, etc.
Object name is gkr430i23.jpg), as well as with the number of non-Hydrogen atoms of the solute. It scales to the cube of the number of points per grid edge, although this expensive cost is attenuated by a preliminary compressing process discarding all points with an excess density close to zero.

For the Solvent-map solvation option, for maps with 65 grid points per edge (about 2 Å grid size), the calculation typically takes from less than a minute to a few minutes for systems of a few thousands atoms to dozens of thousands of atoms (with An external file that holds a picture, illustration, etc.
Object name is gkr430i24.jpg), which is a few times slower than CRYSOL and FoXS. For a WAXS spectrum, An external file that holds a picture, illustration, etc.
Object name is gkr430i25.jpg. For the Surface-Accessible solvation option, computation is more efficient.


Figure 1 shows the flowchart of a typical AquaSAXS calculation. Starting with a PDB file or a PQR file, the user is expected to make two decisions, namely the selection of the solvation method, and whether a comparison is made with an experimental SAXS profile or not.

Figure 1.
Flowchart of AquaSAXS. A PDB or PQR file is mandatory for the calculation, and depending on the chosen method for solvation, a solvent map in CNS format must be fed into the server, or the PDB file must be in PQR format. If one wants to fit a theoretical ...

There are three options for the solvation method: either a solvation map is provided as input, or a solvation map is computed using AquaSol (28), or a hydration layer is defined using the accessible surface of each atom, following the method introduced in FoXS (20).

The Solvent-map solvation option will account for the hydration layer following Equation 4. If a solvent map (in CNS format) is provided by the user in input, it will be taken into account. If no solvent map is provided, AquaSol (28) will be run in default mode prior to AquaSAXS; in that case, the PDB input file must be in PQR format (34) (partial charges and atomic radius must be known for all atoms). The Surface-Accessible solvation option uses a form factor An external file that holds a picture, illustration, etc.
Object name is gkr430i29.jpg defined as in Equation 5.

Optionally, an ASCII text file containing the experimental SAXS profile can be given as input to AquaSAXS. Lines containing data must consist of at least two columns and an optional, but recommended, third one. The first column contains the values of the wavevector q. By default, AquaSAXS defines q as An external file that holds a picture, illustration, etc.
Object name is gkr430i30.jpg in Å−1, where An external file that holds a picture, illustration, etc.
Object name is gkr430i31.jpg is the scattering angle and An external file that holds a picture, illustration, etc.
Object name is gkr430i32.jpg is the wavelength of the incident X-ray beam. Three other definitions of q are also available. The second column contains the scattering intensity values. The optional third column contains the experimental errors. If not provided, the experimental error will be assumed to be equal to the difference between the intensity in qn, and the average intensity at qn1 and qn+1.

The structure file in PDB or PQR format is mandatory. Each line of the file starting with the label ‘ATOM’ or ‘HETATM’ is stored in memory. If the corresponding atom is a hydrogen or belongs to a water molecule, it is discarded. Otherwise, the residue and atom's names are parsed among the standard PDB protein, nucleic acids and ligands library (32). Thirteen atom types are currently recognized: Carbons with zero, one, two or three bound hydrogens, nitrogen with zero, one, two or three bound hydrogens, oxygen with zero or one bound hydrogen, sulfur with zero or one bound hydrogen, and phosphorus. Once the atomic type of atom j has been recognized, its position rj is stored and it is assigned the corresponding form factor fj, excluded volume An external file that holds a picture, illustration, etc.
Object name is gkr430i33.jpg and radius An external file that holds a picture, illustration, etc.
Object name is gkr430i34.jpg. On the ‘Flowchart’ web-page, the user is given the possibility to check whether the atomic types of the residues in the provided PDB/PQR file can be recognized. If the user wants to define other atomic types than those listed above, two optional files can be given as input to the program: one listing the atom types of a given residue, the other listing the atomic parameters of new atom types.

Several other options/parameters can be set: the maximum q-value considered, the sampling resolution of the profile, the bulk average electron density (in e.Å−3), the subset chains in the structure to be considered, as well as the values of C1 and C2 in the nonfitting mode.

The computation is performed in real time and the browser is redirected to the result's page when the calculation has finished. If an email address is provided, an email will be sent to the user. Depending on the system's size and server's queue load, the typical running time ranges from less than a minute to a few minutes. The result's page displays a plot of the computed profile (see Figure 2), superimposed to the experimental profile, if provided, as well as the run logfile. Links to three output files are displayed, to retrieve the logfile, the profile file, and a PDB file listing all the atoms that have been considered in the calculation. Possibly, links toward the output files of AquaSol (28) are displayed too (computed solvent map and logfile).


In addition to single conformation fitting of experimental SAXS profiles, AquaSAXS provides two methods to deal with multiple structure files.

The first one, called ‘Sequential fit’, provides a way to compute the SAXS profile of a set of PDB/PQR files in a single run. That way, if an experimental profile is provided, the user can readily compare the relevance of provided models, without having to rerun the server several times.

The second method, called ‘Ensemble fit’, aims at plugging in all possible models and refining their population, and is directly inspired from (35). This method can prove useful when the macromolecule visits several conformational states in solution. Another interesting way to take profit of this approach would be the following: starting from a given PDB structure, several models are built along a given deformation parameter (e.g. a normal mode); the best value for this deformation parameter would likely be detected by the refinement process.

In practice, this method couples a mean-field optimization of the model's populations with a simulated annealing protocol. Let us consider an ensemble of M models, each model m being associated the scattered intensity An external file that holds a picture, illustration, etc.
Object name is gkr430i37.jpg. Noting pm the associated probability (or population) of model m, the total scattered intensity (in the limit of infinite dilution) is then:

equation image

If one defines a free energy F of the form:

equation image

where An external file that holds a picture, illustration, etc.
Object name is gkr430i39.jpg is defined as in Equation 6, S is the entropy, and T is the temperature, then minimizing the free energy with respect to pm gives:

equation image

where An external file that holds a picture, illustration, etc.
Object name is gkr430i40.jpg is the inverse of An external file that holds a picture, illustration, etc.
Object name is gkr430i41.jpg, An external file that holds a picture, illustration, etc.
Object name is gkr430i42.jpg and An external file that holds a picture, illustration, etc.
Object name is gkr430i43.jpg is a normalization constant given by An external file that holds a picture, illustration, etc.
Object name is gkr430i44.jpg.

The refinement starts with uniform values of the probabilities, which are updated at each cycle of the refinement until a self-consistent solution is obtained; at each cycle the derivative are evaluated at the current solution, i.e. the current set of pm values. The temperature governs the contrast between the different populations, the contrast being higher as the temperature is lower. After convergence at a given temperature, the set of populations found is used as initial guess for a new mean-field refinement at lower temperature, until the final temperature is reached.

Results of both methods applied on synthetic data are presented in Supplementary Materials.


We have described a program that allows structural biologists to compare their SAXS data to the theoretical one for a model given as a PDB or PQR file. Its major novelty resides in the possibility to better model the hydration layer through a physically sound representation of the solvent density map, combined with the use of the cubature method for spherical averaging. The user-friendly interface allows to modify (or add new entries to) the list of scatterers and their parameters.

The possibility to fit the data with multiple models, either independently, or through population refinement has also been implemented.

Future developments will allow for the possibility to refine the coordinates of the model against the experimental data. In that case, care must be taken to use as few degrees of freedom as possible. One possibility is to restrict the deformation of the model along a small number of ‘essential’ normal modes within the framework of the Elastic Network Model (36). Finally, the possibility to compute the theoretical anomalous SAXS profile of a solute containing atoms with anomalous contribution (e.g. Bromide or Cesium ions) will be made available soon.


Supplementary Data are available at NAR Online.


Funding for open access charge: Institut Pasteur.

Conflict of interest statement. None declared.


The authors thank Dr Patrice Vachette for kindly providing the Urate Oxidase's experimental SAXS profile and for helpful suggestions on the manuscript. The authors are grateful to Dr Dominique Durand for her help during beta testing, and to Dr Ludovic Sauguet and Dr Hugues Nury for helpful discussions. P.K. acknowledges support from the NIH.


1. Grossman JG. Biological solution scattering: recent achievements and future challenges. J. Appl. Crystallogr. 2007;40:s217–s222.
2. Glatter O, Kratky O. Small Angle X-ray Scattering. London: Academic; 1982.
3. Guinier A. La diffraction des rayons X aux très petits angles: application l'étude des phénomènes ultramicroscopiques. Ann. Phys. 1939;12:161–237.
4. Doniach S. Changes in biomolecular conformations seen by small-angle X-ray scattering. Chem. Rev. 2001;101:1763–1778. [PubMed]
5. Koch MHJ, Vachette P, Svergun DI. Small-angle scattering: a view on the properties, structures and structural changes of biological macromolecules in solution. Q. Rev. Biophys. 2003;36:147–227. [PubMed]
6. Svergun DI, Koch MHJ. Small-angle scattering studies of biological macromolecules in solution. Rep. Prog. Phys. 2003;66:1735–1782.
7. Lipfert J, Doniach S. Small-angle X-ray scattering from RNA, proteins, and protein complexes. Annu. Rev. Biophys. Biomol. Struct. 2007;36:307–327. [PubMed]
8. Putnam CD, Hammel M, Hura GL, Tainer JA. X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution. Q. Rev. Biophys. 2007;40:191–285. [PubMed]
9. Rambo RP, Tainer JA. Bridging the solution divide: comprehensive structural analyses of dynamic RNA, DNA and protein assemblies by small-angle X-ray scattering. Curr. Opin. Struct. Biol. 2010;20:128–137. [PMC free article] [PubMed]
10. Chacon P, Moran F, Diaz JF, Pantos E, Andreu JM. Low-resolution structure of proteins in solution retrieved from X-ray scattering with a genetic algorithm. Biophys. J. 1998;74:2760–2775. [PMC free article] [PubMed]
11. Svergun DI. Restoring low-resolution structure of biological macromolecules from solution scattering using simulated annealing. Biophys. J. 1999;76:2879–2886. [PMC free article] [PubMed]
12. Pethoukhov MV, Svergun DI. New methods for domain structure determination of proteins from solution scattering data. J. Appl. Crystallogr. 2003;36:540–544.
13. Walther D, Cohen FE, Doniach S. Reconstruction of low resolution three-dimensional density maps from one-dimensional small angle X-ray scattering data for biomolecules in solution. J. Appl. Crystallogr. 2000;33:350–363.
14. Kozin MB, Svergun DI. Automated matching of high- and low-resolution structural models. J. Appl. Crystallogr. 2001;34:33–41.
15. Volkov VV, Svergun DI. Uniqueness of ab initio shape determination in small-angle scattering. J. Appl. Crystallogr. 2003;36:860–864.
16. Wriggers W, Chacon P. Using Situs for the registration of protein structures with low-resolution bead models from X-ray solution scattering. J. Appl. Crystallogr. 2001;34:773–776.
17. Pethoukhov MV, Svergun DI. Global rigid body modeling of macromolecular complexes against small-angle scattering data. Biophys. J. 2005;89:1237–1250. [PMC free article] [PubMed]
18. Förster F, Webb B, Krukenberg KA, Tsuruta H, Agard DA, Sali A. Integration of small-angle X-ray scattering data into structural modeling of proteins and their assemblies. J. Mol. Biol. 2008;382:1089–1106. [PMC free article] [PubMed]
19. Svergun D, Barberato C, Koch MHJ. CRYSOL - a program to evaluate X-ray solution scattering of biological macromolecules from atomic coordinates. J. Appl. Crystallogr. 1995;28:768–773.
20. Schneidman-Duhovny D, Hammel M, Sali A. FoXS: a web server for rapid computation and fitting of SAXS profiles. Nucleic Acids Res. 2010;38:W540–W544. [PMC free article] [PubMed]
21. Tjioe E, Heller WT. ORNL_SAS: software for calculation of small-angle scattering intensities of proteins and proteins complexes. J. Appl. Crystallogr. 2007;40:782–785.
22. Merzel F, Smith JC. SASSIM: a method for calculating small-angle X-ray and neutron scattering and the associated molecular envelope from explicit-atom models of solvated proteins. Acta. Cryst. D. 2002;58:242–249. [PubMed]
23. Yang S, Park S, Makowski L, Roux B. A rapid coarse residue-based computational method for X-ray solution scattering characterization of protein folds and multiple conformational states of large protein complexes. Biophys. J. 2009;96:4449–4463. [PMC free article] [PubMed]
24. Park S, Bardhan JP, Roux B, Makowski L. Simulated X-ray scattering of protein solutions using explicit-solvent models. J. Chem. Phys. 2009;130:134114. [PMC free article] [PubMed]
25. Fraser RDB, MacRae TP, Suzuki E. An improved method for calculating the contribution of solvent to the X-ray diffraction pattern of biological molecules. J. Appl. Crystallogr. 1978;11:693–694.
26. Azuara C, Orland H, Bon M, Koehl P, Delarue M. Incorporating dipolar solvents with variable density in Poisson-Boltzmann electrostatics. Biophys. J. 2008;95:5587–5605. [PubMed]
27. Azuara C, Lindahl E, Koehl P, Orland H, Delarue M. PDB_Hydro: incorporating dipolar solvents with variable density in the Poisson-Boltzmann treatment of macromolecule electrostatics. Nucleic Acids Res. 2006;34:W38–W42. [PMC free article] [PubMed]
28. Koehl P, Delarue M. AQUASOL: an efficient solver for the dipolar Poisson-Boltzmann-Langevin equation. J. Chem. Phys. 2010;132:064101. [PMC free article] [PubMed]
29. Luchko T, Gusarov S, Roe DR, Simmerling C, Case DA, Tuszynski J, Kovalenko A. Three-dimensional molecular theory of solvation coupled with molecular dynamics in Amber. J. Chem. Theo. Comp. 2010;6:607–624. [PMC free article] [PubMed]
30. Fliege J, Maier U. A two-stage approach for computing cubature formulae for the sphere. Technical Report. 1996 Ergebnisberichte Angewandte Mathematik 139T, Universitat Dortmund, Fachbereich Mathematik.
31. Connolly ML. Solvent-accessible surfaces of proteins and nucleic acids. Science. 1983;221:709–713. [PubMed]
32. Berman H, Henrick K, Nakamura H. Announcing the worldwide Protein Data Bank. Nat. Struct. Biol. 2003;10:908. [PubMed]
33. Hura GL, Menon AL, Hammel M, Rambo RP, Poole FL, 2nd, Tsutakawa SE, Jenney FE, Jr, Classen S, Frankel KA, Hopkins RC, et al. Robust, high-throughput solution structural analyses by small angle X-ray scattering (SAXS) Nat. Methods. 2009;6:606–612. [PMC free article] [PubMed]
34. Dolinsky TJ, Nielsen JE, McCammon JA, Baker NA. PDB2PQR: an automated pipeline for the setup of Poisson-Boltzmann electrostatics calculations. Nucleic Acids Res. 2004;32:W665–W667. [PMC free article] [PubMed]
35. Delarue M. Dealing with structural variability in molecular replacement and crystallographic refinement through normal-mode analysis. Acta Cryst. 2008:D40–D48. [PMC free article] [PubMed]
36. Nicolay S, Sanejouand Y-H. Functional modes of proteins are among the most robust. Phys. Rev. Letter. 2006;96:078104–112. [PubMed]

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