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Nucleic Acids Res. Nov 11, 1986; 14(21): 8265–8277.
PMCID: PMC311858

Structure of the RESA gene of Plasmodium falciparum.


We have determined the nucleotide sequence of the gene encoding the ring-infected erythrocyte surface antigen (RESA) of Plasmodium falciparum, an antigen that has been shown to confer protective immunity on monkeys. The sequence has enabled us to predict the structure of the RESA gene and the amino acid sequence of its protein product. The gene consists of two exons with a short intron located near the 5' end of the coding region. A hydrophobic amino acid segment predicted for the 3' end of exon 1 is consistent with the possibility that exon 1 encodes trafficking signal sequences. We show that restriction fragment length polymorphisms can be used to define two different alleles of RESA, represented by isolates FC27 and NF7, and compare the FC27 sequence with that of a long cDNA clone from NF7 described previously.

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Selected References

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  • Coppel RL, Cowman AF, Lingelbach KR, Brown GV, Saint RB, Kemp DJ, Anders RF. Isolate-specific S-antigen of Plasmodium falciparum contains a repeated sequence of eleven amino acids. Nature. 1983 Dec 22;306(5945):751–756. [PubMed]
  • Coppel RL, Cowman AF, Anders RF, Bianco AE, Saint RB, Lingelbach KR, Kemp DJ, Brown GV. Immune sera recognize on erythrocytes Plasmodium falciparum antigen composed of repeated amino acid sequences. Nature. 310(5980):789–792. [PubMed]
  • Stahl HD, Crewther PE, Anders RF, Brown GV, Coppel RL, Bianco AE, Mitchell GF, Kemp DJ. Interspersed blocks of repetitive and charged amino acids in a dominant immunogen of Plasmodium falciparum. Proc Natl Acad Sci U S A. 1985 Jan;82(2):543–547. [PMC free article] [PubMed]
  • Koenen M, Scherf A, Mercereau O, Langsley G, Sibilli L, Dubois P, Pereira da Silva L, Müller-Hill B. Human antisera detect a Plasmodium falciparum genomic clone encoding a nonapeptide repeat. Nature. 311(5984):382–385. [PubMed]
  • Kemp DJ, Coppel RL, Cowman AF, Saint RB, Brown GV, Anders RF. Expression of Plasmodium falciparum blood-stage antigens in Escherichia coli: detection with antibodies from immune humans. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3787–3791. [PMC free article] [PubMed]
  • Brown GV, Culvenor JG, Crewther PE, Bianco AE, Coppel RL, Saint RB, Stahl HD, Kemp DJ, Anders RF. Localization of the ring-infected erythrocyte surface antigen (RESA) of Plasmodium falciparum in merozoites and ring-infected erythrocytes. J Exp Med. 1985 Aug 1;162(2):774–779. [PMC free article] [PubMed]
  • Perlmann H, Berzins K, Wahlgren M, Carlsson J, Björkman A, Patarroyo ME, Perlmann P. Antibodies in malarial sera to parasite antigens in the membrane of erythrocytes infected with early asexual stages of Plasmodium falciparum. J Exp Med. 1984 Jun 1;159(6):1686–1704. [PMC free article] [PubMed]
  • Cowman AF, Coppel RL, Saint RB, Favaloro J, Crewther PE, Stahl HD, Bianco AE, Brown GV, Anders RF, Kemp DJ. The ring-infected erythrocyte surface antigen (RESA) polypeptide of Plasmodium falciparum contains two separate blocks of tandem repeats encoding antigenic epitopes that are naturally immunogenic in man. Mol Biol Med. 1984 Jun;2(3):207–221. [PubMed]
  • Kochan J, Perkins M, Ravetch JV. A tandemly repeated sequence determines the binding domain for an erythrocyte receptor binding protein of P. falciparum. Cell. 1986 Mar 14;44(5):689–696. [PubMed]
  • Dame JB, Williams JL, McCutchan TF, Weber JL, Wirtz RA, Hockmeyer WT, Maloy WL, Haynes JD, Schneider I, Roberts D, et al. Structure of the gene encoding the immunodominant surface antigen on the sporozoite of the human malaria parasite Plasmodium falciparum. Science. 1984 Aug 10;225(4662):593–599. [PubMed]
  • Kemp DJ, Coppel RL, Stahl HD, Bianco AE, Corcoran LM, McIntyre P, Langford CJ, Favaloro JM, Crewther PE, Brown GV, et al. The Wellcome Trust lecture. Genes for antigens of Plasmodium falciparum. Parasitology. 1986;92 (Suppl):S83–108. [PubMed]
  • Wåhlin B, Wahlgren M, Perlmann H, Berzins K, Björkman A, Patarroyo ME, Perlmann P. Human antibodies to a Mr 155,000 Plasmodium falciparum antigen efficiently inhibit merozoite invasion. Proc Natl Acad Sci U S A. 1984 Dec;81(24):7912–7916. [PMC free article] [PubMed]
  • Berzins K, Perlmann H, Wåhlin B, Carlsson J, Wahlgren M, Udomsangpetch R, Björkman A, Patarroyo ME, Perlmann P. Rabbit and human antibodies to a repeated amino acid sequence of a Plasmodium falciparum antigen, Pf 155, react with the native protein and inhibit merozoite invasion. Proc Natl Acad Sci U S A. 1986 Feb;83(4):1065–1069. [PMC free article] [PubMed]
  • Cowman AF, Saint RB, Coppel RL, Brown GV, Anders RF, Kemp DJ. Conserved sequences flank variable tandem repeats in two S-antigen genes of Plasmodium falciparum. Cell. 1985 Apr;40(4):775–783. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Anders RF, Shi PT, Scanlon DB, Leach SJ, Coppel RL, Brown GV, Stahl HD, Kemp DJ. Antigenic repeat structures in proteins of Plasmodium falciparum. Ciba Found Symp. 1986;119:164–183. [PubMed]
  • Stahl HD, Kemp DJ, Crewther PE, Scanlon DB, Woodrow G, Brown GV, Bianco AE, Anders RF, Coppel RL. Sequence of a cDNA encoding a small polymorphic histidine- and alanine-rich protein from Plasmodium falciparum. Nucleic Acids Res. 1985 Nov 11;13(21):7837–7846. [PMC free article] [PubMed]
  • Eisenberg D, Schwarz E, Komaromy M, Wall R. Analysis of membrane and surface protein sequences with the hydrophobic moment plot. J Mol Biol. 1984 Oct 15;179(1):125–142. [PubMed]
  • DeLisi C, Berzofsky JA. T-cell antigenic sites tend to be amphipathic structures. Proc Natl Acad Sci U S A. 1985 Oct;82(20):7048–7052. [PMC free article] [PubMed]
  • Kaul RK, Murthy SN, Reddy AG, Steck TL, Kohler H. Amino acid sequence of the N alpha-terminal 201 residues of human erythrocyte membrane band 3. J Biol Chem. 1983 Jul 10;258(13):7981–7990. [PubMed]
  • Dekowski SA, Rybicki A, Drickamer K. A tyrosine kinase associated with the red cell membrane phosphorylates band 3. J Biol Chem. 1983 Mar 10;258(5):2750–2753. [PubMed]
  • Bennett V. The membrane skeleton of human erythrocytes and its implications for more complex cells. Annu Rev Biochem. 1985;54:273–304. [PubMed]
  • Staden R. Graphic methods to determine the function of nucleic acid sequences. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):521–538. [PMC free article] [PubMed]

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