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Nucleic Acids Res. Jun 11, 1986; 14(11): 4617–4624.
PMCID: PMC311469

Expression in mammalian cells of a gene from Streptomyces alboniger conferring puromycin resistance.


The gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger has been cloned next to the SV40 early promoter in a mammalian cells-Escherichia coli shuttle vector. When this construction was introduced into VERO cells it expressed the relevant enzymic activity. Moreover, the puromycin N-acetyl transferase gene has been used as a dominant marker for the selection of transformed mammalian cells able to grow in the presence of the antibiotic.

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Selected References

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