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Nucleic Acids Res. 1993 Nov 11; 21(22): 5283–5284.
PMCID: PMC310653

PCR amplification of highly GC-rich DNA template after denaturation by NaOH.


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Selected References

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  • Erlich HA, Gelfand D, Sninsky JJ. Recent advances in the polymerase chain reaction. Science. 1991 Jun 21;252(5013):1643–1651. [PubMed]
  • Sarkar G, Kapelner S, Sommer SS. Formamide can dramatically improve the specificity of PCR. Nucleic Acids Res. 1990 Dec 25;18(24):7465–7465. [PMC free article] [PubMed]
  • Dutton CM, Paynton C, Sommer SS. General method for amplifying regions of very high G+C content. Nucleic Acids Res. 1993 Jun 25;21(12):2953–2954. [PMC free article] [PubMed]
  • Perl A, Rosenblatt JD, Chen IS, DiVincenzo JP, Bever R, Poiesz BJ, Abraham GN. Detection and cloning of new HTLV-related endogenous sequences in man. Nucleic Acids Res. 1989 Sep 12;17(17):6841–6854. [PMC free article] [PubMed]
  • Banki K, Maceda J, Hurley E, Ablonczy E, Mattson DH, Szegedy L, Hung C, Perl A. Human T-cell lymphotropic virus (HTLV)-related endogenous sequence, HRES-1, encodes a 28-kDa protein: a possible autoantigen for HTLV-I gag-reactive autoantibodies. Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1939–1943. [PMC free article] [PubMed]
  • Srivastava BI, Banki K, Perl A. Human T-cell leukemia virus type I or a related retrovirus in patients with mycosis fungoides/Sézary syndrome and Kaposi's sarcoma. Cancer Res. 1992 Aug 15;52(16):4391–4395. [PubMed]
  • Shimotohno K, Takahashi Y, Shimizu N, Gojobori T, Golde DW, Chen IS, Miwa M, Sugimura T. Complete nucleotide sequence of an infectious clone of human T-cell leukemia virus type II: an open reading frame for the protease gene. Proc Natl Acad Sci U S A. 1985 May;82(10):3101–3105. [PMC free article] [PubMed]

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