• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of narLink to Publisher's site
Nucleic Acids Res. Feb 11, 1992; 20(3): 624.
PMCID: PMC310447

A PCR method for introducing mutations into cloned DNA by joining an internal primer to a tagged flanking primer.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (204K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Kemler R, Ozawa M, Ringwald M. Calcium-dependent cell adhesion molecules. Curr Opin Cell Biol. 1989 Oct;1(5):892–897. [PubMed]
  • Nossal NG. DNA synthesis on a double-stranded DNA template by the T4 bacteriophage DNA polymerase and the T4 gene 32 DNA unwinding protein. J Biol Chem. 1974 Sep 10;249(17):5668–5676. [PubMed]
  • Kunkel TA. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc Natl Acad Sci U S A. 1985 Jan;82(2):488–492. [PMC free article] [PubMed]
  • Perlak FJ. Single step large scale site-directed in vitro mutagenesis using multiple oligonucleotides. Nucleic Acids Res. 1990 Dec 25;18(24):7457–7458. [PMC free article] [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Compound
    PubChem Compound links
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem Substance links