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Nucleic Acids Res. 1992 Feb 11; 20(3): 624.
PMCID: PMC310447

A PCR method for introducing mutations into cloned DNA by joining an internal primer to a tagged flanking primer.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Kemler R, Ozawa M, Ringwald M. Calcium-dependent cell adhesion molecules. Curr Opin Cell Biol. 1989 Oct;1(5):892–897. [PubMed]
  • Nossal NG. DNA synthesis on a double-stranded DNA template by the T4 bacteriophage DNA polymerase and the T4 gene 32 DNA unwinding protein. J Biol Chem. 1974 Sep 10;249(17):5668–5676. [PubMed]
  • Kunkel TA. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc Natl Acad Sci U S A. 1985 Jan;82(2):488–492. [PMC free article] [PubMed]
  • Perlak FJ. Single step large scale site-directed in vitro mutagenesis using multiple oligonucleotides. Nucleic Acids Res. 1990 Dec 25;18(24):7457–7458. [PMC free article] [PubMed]

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