• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of narLink to Publisher's site
Nucleic Acids Res. Aug 11, 1994; 22(15): 3099–3103.
PMCID: PMC310281

Chromatin structure determines the sites of chromosome breakages in Plasmodium falciparum.

Abstract

Spontaneous chromosome breakages are frequently observed in the human malaria parasite Plasmodium falciparum and are responsible for the generation of novel phenotypes, which may contribute to the pathogenicity and virulence of this protozoan parasite. The identification of a hot spot of chromosome breakage within the coding region of the KAHRP gene revealed that these events do not occur randomly but follow a regular pattern with a periodicity of 155 bp. This phasing corresponds to the average repeat unit of P. falciparum nucleosomes. Furthermore, breakage events preferentially occur within the linker regions of nucleosomes, as demonstrated by mapping endonuclease hypersensitive sites of chromatin. These data suggest that, in P. falciparum, the chromatin structure is involved in the molecular process of chromosome breakage, a mechanism that may be common in other eukaryotes.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Trager W, Jensen JB. Human malaria parasites in continuous culture. Science. 1976 Aug 20;193(4254):673–675. [PubMed]
  • de Bruin D, Lanzer M, Ravetch JV. Characterization of yeast artificial chromosomes from Plasmodium falciparum: construction of a stable, representative library and cloning of telomeric DNA fragments. Genomics. 1992 Oct;14(2):332–339. [PubMed]
  • Wright JH, Gottschling DE, Zakian VA. Saccharomyces telomeres assume a non-nucleosomal chromatin structure. Genes Dev. 1992 Feb;6(2):197–210. [PubMed]
  • Pologe LG, Pavlovec A, Shio H, Ravetch JV. Primary structure and subcellular localization of the knob-associated histidine-rich protein of Plasmodium falciparum. Proc Natl Acad Sci U S A. 1987 Oct;84(20):7139–7143. [PMC free article] [PubMed]
  • Creedon KA, Kaslow DC, Rathod PK, Wellems TE. Identification of a Plasmodium falciparum histone 2A gene. Mol Biochem Parasitol. 1992 Aug;54(1):113–115. [PubMed]
  • Adams CC, Workman JL. Nucleosome displacement in transcription. Cell. 1993 Feb 12;72(3):305–308. [PubMed]
  • Clark DJ, Felsenfeld G. A nucleosome core is transferred out of the path of a transcribing polymerase. Cell. 1992 Oct 2;71(1):11–22. [PubMed]
  • van Holde KE, Lohr DE, Robert C. What happens to nucleosomes during transcription? J Biol Chem. 1992 Feb 15;267(5):2837–2840. [PubMed]
  • Bresnick EH, Bustin M, Marsaud V, Richard-Foy H, Hager GL. The transcriptionally-active MMTV promoter is depleted of histone H1. Nucleic Acids Res. 1992 Jan 25;20(2):273–278. [PMC free article] [PubMed]
  • Nacheva GA, Guschin DY, Preobrazhenskaya OV, Karpov VL, Ebralidse KK, Mirzabekov AD. Change in the pattern of histone binding to DNA upon transcriptional activation. Cell. 1989 Jul 14;58(1):27–36. [PubMed]
  • Gottesfeld JM, Melton DA. The length of nucleosome-associated DNA is the same in both transcribed and nontranscribed regions of chromatin. Nature. 1978 May 25;273(5660):317–319. [PubMed]
  • Studitsky VM, Clark DJ, Felsenfeld G. A histone octamer can step around a transcribing polymerase without leaving the template. Cell. 1994 Jan 28;76(2):371–382. [PubMed]
  • Vernick KD, McCutchan TF. Sequence and structure of a Plasmodium falciparum telomere. Mol Biochem Parasitol. 1988 Mar;28(2):85–94. [PubMed]
  • Collins K, Greider CW. Tetrahymena telomerase catalyzes nucleolytic cleavage and nonprocessive elongation. Genes Dev. 1993 Jul;7(7B):1364–1376. [PubMed]
  • Harrington LA, Greider CW. Telomerase primer specificity and chromosome healing. Nature. 1991 Oct 3;353(6343):451–454. [PubMed]
  • Morin GB. Recognition of a chromosome truncation site associated with alpha-thalassaemia by human telomerase. Nature. 1991 Oct 3;353(6343):454–456. [PubMed]
  • Wilkie AO, Lamb J, Harris PC, Finney RD, Higgs DR. A truncated human chromosome 16 associated with alpha thalassaemia is stabilized by addition of telomeric repeat (TTAGGG)n. Nature. 1990 Aug 30;346(6287):868–871. [PubMed]
  • Wu TC, Lichten M. Meiosis-induced double-strand break sites determined by yeast chromatin structure. Science. 1994 Jan 28;263(5146):515–518. [PubMed]
  • Roberts DJ, Craig AG, Berendt AR, Pinches R, Nash G, Marsh K, Newbold CI. Rapid switching to multiple antigenic and adhesive phenotypes in malaria. Nature. 1992 Jun 25;357(6380):689–692. [PMC free article] [PubMed]
  • Kemp DJ, Corcoran LM, Coppel RL, Stahl HD, Bianco AE, Brown GV, Anders RF. Size variation in chromosomes from independent cultured isolates of Plasmodium falciparum. Nature. 1985 May 23;315(6017):347–350. [PubMed]
  • Van der Ploeg LH, Smits M, Ponnudurai T, Vermeulen A, Meuwissen JH, Langsley G. Chromosome-sized DNA molecules of Plasmodium falciparum. Science. 1985 Aug 16;229(4714):658–661. [PubMed]
  • Pologe LG, Ravetch JV. A chromosomal rearrangement in a P. falciparum histidine-rich protein gene is associated with the knobless phenotype. Nature. 322(6078):474–477. [PubMed]
  • Pologe LG, Ravetch JV. Large deletions result from breakage and healing of P. falciparum chromosomes. Cell. 1988 Dec 2;55(5):869–874. [PubMed]
  • Borst P, Greaves DR. Programmed gene rearrangements altering gene expression. Science. 1987 Feb 6;235(4789):658–667. [PubMed]
  • Lanzer M, de Bruin D, Ravetch JV. Transcriptional differences in polymorphic and conserved domains of a complete cloned P. falciparum chromosome. Nature. 1993 Feb 18;361(6413):654–657. [PubMed]
  • Pologe LG, de Bruin D, Ravetch JV. A and T homopolymeric stretches mediate a DNA inversion in Plasmodium falciparum which results in loss of gene expression. Mol Cell Biol. 1990 Jun;10(6):3243–3246. [PMC free article] [PubMed]
  • Scherf A, Mattei D. Cloning and characterization of chromosome breakpoints of Plasmodium falciparum: breakage and new telomere formation occurs frequently and randomly in subtelomeric genes. Nucleic Acids Res. 1992 Apr 11;20(7):1491–1496. [PMC free article] [PubMed]
  • Shirley MW, Biggs BA, Forsyth KP, Brown HJ, Thompson JK, Brown GV, Kemp DJ. Chromosome 9 from independent clones and isolates of Plasmodium falciparum undergoes subtelomeric deletions with similar breakpoints in vitro. Mol Biochem Parasitol. 1990 Apr;40(1):137–145. [PubMed]
  • Scherf A, Carter R, Petersen C, Alano P, Nelson R, Aikawa M, Mattei D, Pereira da Silva L, Leech J. Gene inactivation of Pf11-1 of Plasmodium falciparum by chromosome breakage and healing: identification of a gametocyte-specific protein with a potential role in gametogenesis. EMBO J. 1992 Jun;11(6):2293–2301. [PMC free article] [PubMed]
  • Raventos-Suarez C, Kaul DK, Macaluso F, Nagel RL. Membrane knobs are required for the microcirculatory obstruction induced by Plasmodium falciparum-infected erythrocytes. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3829–3833. [PMC free article] [PubMed]
  • Barnwell JW. Cytoadherence and sequestration in falciparum malaria. Exp Parasitol. 1989 Nov;69(4):407–412. [PubMed]
  • Udeinya IJ, Schmidt JA, Aikawa M, Miller LH, Green I. Falciparum malaria-infected erythrocytes specifically bind to cultured human endothelial cells. Science. 1981 Jul 31;213(4507):555–557. [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...