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Journal List > Nucleic Acids Res > v.22(15); Aug 11, 1994

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Nucleic Acids Res. 1994 August 11; 22(15): 3005–3010.
PMCID: PMC310268
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Role of the human ERCC-1 gene in gene-specific repair of cisplatin-induced DNA damage.
F Larminat and V A Bohr
Laboratory of Molecular Genetics, National Institute on Aging, NIH, Baltimore, MD 21224.
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Abstract
The human excision repair gene ERCC-1 gene restores normal resistance to UV and mitomycin C in excision repair deficient chinese hamster ovary cells of complementation group 1. To investigate the involvement of the ERCC-1 gene in gene-specific repair of bulky lesions, we have studied the removal of damage induced by the antitumor agent cisplatin in CHO mutant 43-3B cells of group 1, with or without transfection with the ERCC-1 gene. Firstly, we determined the contribution of the ERCC-1 gene to the repair of interstrand crosslinks (ICL) induced by cisplatin and found efficient removal of ICLs from the dihydrofolate reductase (DHFR) gene in the ERCC-1 transfected 43-3B cells. We then assessed the contribution of ERCC-1 to the repair of intrastrand adducts (IA) induced by cisplatin. Compared to the wild-type parental cell line, the ERCC-1 transfected 43-3B cells repaired the IAs in the DHFR gene inefficiently. Thus, our data suggest that the ERCC-1 gene is more involved in the repair of interstrand crosslinks than in the removal of intrastrand adducts.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
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