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Nucleic Acids Res. Jan 11, 1993; 21(1): 173–174.
PMCID: PMC309083

Large-scale production of DNA sequencing templates by microtitre format PCR.


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Selected References

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  • Smith V, Brown CM, Bankier AT, Barrell BG. Semiautomated preparation of DNA templates for large-scale sequencing projects. DNA Seq. 1990;1(1):73–78. [PubMed]
  • Mardis ER, Roe BA. Automated methods for single-stranded DNA isolation and dideoxynucleotide DNA sequencing reactions on a robotic workstation. Biotechniques. 1989 Sep;7(8):840–850. [PubMed]
  • Fry G, Lachenmeier E, Mayrand E, Giusti B, Fisher J, Johnston-Dow L, Cathcart R, Finne E, Kilaas L. A new approach to template purification for sequencing applications using paramagnetic particles. Biotechniques. 1992 Jul;13(1):124–131. [PubMed]
  • Hawkins T. M13 single-strand purification using a biotinylated probe and streptavidin coated magnetic beads. DNA Seq. 1992;3(2):65–69. [PubMed]
  • Gyllensten UB, Erlich HA. Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus. Proc Natl Acad Sci U S A. 1988 Oct;85(20):7652–7656. [PMC free article] [PubMed]
  • Güssow D, Clackson T. Direct clone characterization from plaques and colonies by the polymerase chain reaction. Nucleic Acids Res. 1989 May 25;17(10):4000–4000. [PMC free article] [PubMed]
  • Schofield JP, Vaudin M, Kettle S, Jones DS. A rapid semi-automated microtiter plate method for analysis and sequencing by PCR from bacterial stocks. Nucleic Acids Res. 1989 Nov 25;17(22):9498–9498. [PMC free article] [PubMed]
  • Hultman T, Ståhl S, Hornes E, Uhlén M. Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucleic Acids Res. 1989 Jul 11;17(13):4937–4946. [PMC free article] [PubMed]
  • Waterston R, Martin C, Craxton M, Huynh C, Coulson A, Hillier L, Durbin R, Green P, Shownkeen R, Halloran N, et al. A survey of expressed genes in Caenorhabditis elegans. Nat Genet. 1992 May;1(2):114–123. [PubMed]
  • Rosenthal A, Charnock-Jones DS. New protocols for DNA sequencing with dye terminators. DNA Seq. 1992;3(1):61–64. [PubMed]

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