• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of narLink to Publisher's site
Nucleic Acids Res. Sep 11, 1994; 22(18): 3715–3721.
PMCID: PMC308352

The DNA polymerase genes of several HMU-bacteriophages have similar group I introns with highly divergent open reading frames.

Abstract

A previous report described the discovery of a group I, self-splicing intron in the DNA polymerase gene of the Bacillus subtilis bacteriophage SPO1 (1). In this study, the DNA polymerase genes of three close relatives of SPO1: SP82, 2C and phi e, were also found to be interrupted by an intron. All of these introns have group I secondary structures that are extremely similar to one another in primary sequence. Each is interrupted by an open reading frame (ORF) that, unlike the intron core or exon sequences, are highly diverged. Unlike the relatives of Escherichia coli bacteriophage T4, most of which do not have introns (2), this intron seems to be common among the relatives of SPO1.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.7M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Goodrich-Blair H, Scarlato V, Gott JM, Xu MQ, Shub DA. A self-splicing group I intron in the DNA polymerase gene of Bacillus subtilis bacteriophage SPO1. Cell. 1990 Oct 19;63(2):417–424. [PubMed]
  • Lambowitz AM, Belfort M. Introns as mobile genetic elements. Annu Rev Biochem. 1993;62:587–622. [PubMed]
  • Cech TR. Conserved sequences and structures of group I introns: building an active site for RNA catalysis--a review. Gene. 1988 Dec 20;73(2):259–271. [PubMed]
  • Michel F, Westhof E. Modelling of the three-dimensional architecture of group I catalytic introns based on comparative sequence analysis. J Mol Biol. 1990 Dec 5;216(3):585–610. [PubMed]
  • Cech TR. Self-splicing of group I introns. Annu Rev Biochem. 1990;59:543–568. [PubMed]
  • Mota EM, Collins RA. Independent evolution of structural and coding regions in a Neurospora mitochondrial intron. Nature. 1988 Apr 14;332(6165):654–656. [PubMed]
  • Garriga G, Lambowitz AM. RNA splicing in neurospora mitochondria: self-splicing of a mitochondrial intron in vitro. Cell. 1984 Dec;39(3 Pt 2):631–641. [PubMed]
  • Gott JM, Shub DA, Belfort M. Multiple self-splicing introns in bacteriophage T4: evidence from autocatalytic GTP labeling of RNA in vitro. Cell. 1986 Oct 10;47(1):81–87. [PubMed]
  • Shub DA, Gott JM, Xu MQ, Lang BF, Michel F, Tomaschewski J, Pedersen-Lane J, Belfort M. Structural conservation among three homologous introns of bacteriophage T4 and the group I introns of eukaryotes. Proc Natl Acad Sci U S A. 1988 Feb;85(4):1151–1155. [PMC free article] [PubMed]
  • Sjöberg BM, Hahne S, Mathews CZ, Mathews CK, Rand KN, Gait MJ. The bacteriophage T4 gene for the small subunit of ribonucleotide reductase contains an intron. EMBO J. 1986 Aug;5(8):2031–2036. [PMC free article] [PubMed]
  • Young P, Ohman M, Xu MQ, Shub DA, Sjöberg BM. Intron-containing T4 bacteriophage gene sunY encodes an anaerobic ribonucleotide reductase. J Biol Chem. 1994 Aug 12;269(32):20229–20232. [PubMed]
  • GREEN DM. INFECTIVITY OF DNA ISOLATED FROM BACILLUS SUBTILIS BACTERIOPHAGE, SP82. J Mol Biol. 1964 Dec;10:438–451. [PubMed]
  • Roscoe DH, Tucker RG. The biosynthesis of 5-hydroxymethyldeoxyuridylic acid in bacteriophage-infected Bacillus subtilis. Virology. 1966 May;29(1):157–166. [PubMed]
  • ROMIG WR, BRODETSKY AM. Isolation and preliminary characterization of bacteriophages for Bacillus subtilis. J Bacteriol. 1961 Jul;82:135–141. [PMC free article] [PubMed]
  • Scarlato V, Gargano S. The DNA polymerase-encoding gene of Bacillus subtilis bacteriophage SPO1. Gene. 1992 Sep 1;118(1):109–113. [PubMed]
  • Gage LP, Geiduschek EP. RNA synthesis during bacteriophage SPO1 development: six classes of SPO1 RNA. J Mol Biol. 1971 Apr 28;57(2):279–297. [PubMed]
  • Braithwaite DK, Ito J. Compilation, alignment, and phylogenetic relationships of DNA polymerases. Nucleic Acids Res. 1993 Feb 25;21(4):787–802. [PMC free article] [PubMed]
  • Zuker M, Jaeger JA, Turner DH. A comparison of optimal and suboptimal RNA secondary structures predicted by free energy minimization with structures determined by phylogenetic comparison. Nucleic Acids Res. 1991 May 25;19(10):2707–2714. [PMC free article] [PubMed]
  • Trinkl H, Wolf K. The mosaic cox1 gene in the mitochondrial genome of Schizosaccharomyces pombe: minimal structural requirements and evolution of group I introns. Gene. 1986;45(3):289–297. [PubMed]
  • Cummings DJ, Michel F, McNally KL. DNA sequence analysis of the 24.5 kilobase pair cytochrome oxidase subunit I mitochondrial gene from Podospora anserina: a gene with sixteen introns. Curr Genet. 1989 Dec;16(5-6):381–406. [PubMed]
  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. [PubMed]
  • Weise F, Chai S, Lüder G, Alonso JC. Nucleotide sequence and complementation studies of the gene 35 region of the Bacillus subtilis bacteriophage SPP1. Virology. 1994 Aug 1;202(2):1046–1049. [PubMed]
  • Perlman PS, Butow RA. Mobile introns and intron-encoded proteins. Science. 1989 Dec 1;246(4934):1106–1109. [PubMed]
  • Sharma M, Ellis RL, Hinton DM. Identification of a family of bacteriophage T4 genes encoding proteins similar to those present in group I introns of fungi and phage. Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6658–6662. [PMC free article] [PubMed]
  • Shub DA, Goodrich-Blair H. Protein introns: a new home for endonucleases. Cell. 1992 Oct 16;71(2):183–186. [PubMed]
  • Truffaut N, Revet B, Soulie MO. Etude comparative des DNA de phages 2C, SP8*, SP82, phi e, SP01 et SP50. Eur J Biochem. 1970 Aug;15(2):391–400. [PubMed]
  • Lawrie JM, Downard JS, Whiteley HR. Bacillus subtilis bacteriophages SP82, SPO1, and phie: a comparison of DNAs and of peptides synthesized during infection. J Virol. 1978 Sep;27(3):725–737. [PMC free article] [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...