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PMC full text:
J Am Chem Soc. Author manuscript; available in PMC 2012 Jun 14.
Published in final edited form as:
J Am Chem Soc. 2011 Jan 26; 133(3): 486–492.
Published online 2010 Dec 14. doi:  10.1021/ja107836t

Figure 4

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Temperature dependence on stability of duplexes with different cytosine modifications. (a) Mean transport times for 3 kbp C-DNA, mC-DNA, and hmC-DNA samples as a function of bath temperature. With increasing temperatures in the range of 10–30°C, mean transport times decrease for mC-DNA and C-DNA, while increasing for hmC-DNA. (b) Fluorescence annealing curves for three DNA molecules. The curves were obtained by differentiating the fluorescence signal from SYBR Green I® in the presence of DNA while cooling from 98°C to 54°C in decrements of 0.2°C using a realtime PCR instrument (curves were shifted for clarity). The peak positions represent the annealing temperatures Tm, written above each curve. Tm values follow the trend hmC-DNA<C- DNA<mC-DNA. In addition, annealing of hmC-DNA occurs more gradually with temperature than for C-DNA or mC-DNA, as shown in the inset plot of T-Tm.

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