Logo of narLink to Publisher's site
Nucleic Acids Res. Jun 11, 1994; 22(11): 2168–2169.
PMCID: PMC308138

Rapid preparation of megabase plant DNA from nuclei in agarose plugs and microbeads.


Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (412K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Guidet F, Rogowsky P, Langridge P. A rapid method of preparing megabase plant DNA. Nucleic Acids Res. 1990 Aug 25;18(16):4955–4955. [PMC free article] [PubMed]
  • Cook PR. A general method for preparing intact nuclear DNA. EMBO J. 1984 Aug;3(8):1837–1842. [PMC free article] [PubMed]
  • Koob M, Szybalski W. Preparing and using agarose microbeads. Methods Enzymol. 1992;216:13–20. [PubMed]
  • Timberlake WE. Low repetitive DNA content in Aspergillus nidulans. Science. 1978 Dec 1;202(4371):973–975. [PubMed]
  • Fischer RL, Goldberg RB. Structure and flanking regions of soybean seed protein genes. Cell. 1982 Jun;29(2):651–660. [PubMed]
  • Guzmán P, Ecker JR. Development of large DNA methods for plants: molecular cloning of large segments of Arabidopsis and carrot DNA into yeast. Nucleic Acids Res. 1988 Dec 9;16(23):11091–11105. [PMC free article] [PubMed]
  • Cheung WY, Gale MD. The isolation of high molecular weight DNA from wheat, barley and rye for analysis by pulse-field gel electrophoresis. Plant Mol Biol. 1990 May;14(5):881–888. [PubMed]
  • Guidet F, Langridge P. Megabase DNA preparation from plant tissue. Methods Enzymol. 1992;216:3–12. [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Compound
    PubChem Compound links
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...