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Nucleic Acids Res. Jun 11, 1994; 22(11): 2168–2169.
PMCID: PMC308138

Rapid preparation of megabase plant DNA from nuclei in agarose plugs and microbeads.

Abstract

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Selected References

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  • Guidet F, Rogowsky P, Langridge P. A rapid method of preparing megabase plant DNA. Nucleic Acids Res. 1990 Aug 25;18(16):4955–4955. [PMC free article] [PubMed]
  • Cook PR. A general method for preparing intact nuclear DNA. EMBO J. 1984 Aug;3(8):1837–1842. [PMC free article] [PubMed]
  • Koob M, Szybalski W. Preparing and using agarose microbeads. Methods Enzymol. 1992;216:13–20. [PubMed]
  • Timberlake WE. Low repetitive DNA content in Aspergillus nidulans. Science. 1978 Dec 1;202(4371):973–975. [PubMed]
  • Fischer RL, Goldberg RB. Structure and flanking regions of soybean seed protein genes. Cell. 1982 Jun;29(2):651–660. [PubMed]
  • Guzmán P, Ecker JR. Development of large DNA methods for plants: molecular cloning of large segments of Arabidopsis and carrot DNA into yeast. Nucleic Acids Res. 1988 Dec 9;16(23):11091–11105. [PMC free article] [PubMed]
  • Cheung WY, Gale MD. The isolation of high molecular weight DNA from wheat, barley and rye for analysis by pulse-field gel electrophoresis. Plant Mol Biol. 1990 May;14(5):881–888. [PubMed]
  • Guidet F, Langridge P. Megabase DNA preparation from plant tissue. Methods Enzymol. 1992;216:3–12. [PubMed]

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