• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of narLink to Publisher's site
Nucleic Acids Res. Oct 11, 1995; 23(19): 3805–3809.
PMCID: PMC307294

Induction of double-strand breaks by S1 nuclease, mung bean nuclease and nuclease P1 in DNA containing abasic sites and nicks.

Abstract

Defined DNA substrates containing discrete abasic sites or paired abasic sites set 1, 3, 5 and 7 bases apart on opposite strands were constructed to examine the reactivity of S1, mung bean and P1 nucleases towards abasic sites. None of the enzymes acted on the substrate containing discrete abasic sites. Under conditions where little or no non-specific DNA degradation was observed, all three nucleases were able to generate double-strand breaks when the bistranded abasic sites were 1 and 3 base pairs apart. However, when the abasic sites were further apart, the enzymes again failed to cleave the DNA. These results indicate that single abasic sites do not cause sufficient denaturation of the DNA to allow incision by these single-strand specific endonucleases. The reactivity of these enzymes was also investigated on DNA substrates that were nicked by DNasel or more site-specifically by endonuclease III incision at the discrete abasic sites. The three nucleases readily induced a strand break opposite such nicks.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Rushizky GW. S1 nuclease of Aspergillus oryzae. Gene Amplif Anal. 1981;2:205–215. [PubMed]
  • Shishido K. Relationship between S1 endonuclease-sensitivity and number of superhelical turns in a negatively-twisted DNA. FEBS Lett. 1980 Mar 10;111(2):333–336. [PubMed]
  • Ghangas GS, Wu R. Specific hydrolysis of the cohesive ends of bacteriophage lambda DNA by three single strand-specific nucleases. J Biol Chem. 1975 Jun 25;250(12):4601–4606. [PubMed]
  • Kroeker WD, Kowalski D. Gene-sized pieces produced by digestion of linear duplex DNA with mung bean nuclease. Biochemistry. 1978 Aug 8;17(16):3236–3243. [PubMed]
  • Kroeker WD, Kowalski D, Laskowski M., Sr Mung bean nuclease I. Terminally directed hydrolysis of native DNA. Biochemistry. 1976 Oct 5;15(20):4463–4467. [PubMed]
  • Wang JC. Interactions between twisted DNAs and enzymes: the effects of superhelical turns. J Mol Biol. 1974 Aug 25;87(4):797–816. [PubMed]
  • Kowalski D, Sanford JP. Action of mung bean nuclease on supercoiled PM2 DNA. J Biol Chem. 1982 Jul 10;257(13):7820–7825. [PubMed]
  • Kazumi T, Yoshino G, Fujii S, Baba S. Tumorigenic action of streptozotocin on the pancreas and kidney in male Wistar rats. Cancer Res. 1978 Jul;38(7):2144–2147. [PubMed]
  • Shishido K, Ando T. Cleavage of ultraviolet light-irradiated DNA by single strand-specific S1 endonuclease. Biochem Biophys Res Commun. 1974 Aug 19;59(4):1380–1388. [PubMed]
  • Rizvi RY, Alvi NK, Hadi SM. Effect of alkylation on the secondary structure of DNA. Biosci Rep. 1982 May;2(5):315–322. [PubMed]
  • Rizvi RY, Shahabuddin, Rahman A, Hadi SM. Effect of alkylation with streptozotocin on the secondary structure of DNA. Biosci Rep. 1986 Jun;6(6):557–564. [PubMed]
  • Butour JL, Mazard AM, Vieussens C, Johnson NP. Kinetic studies of the hydrolysis of platinum-DNA complexes by nuclease S1. Chem Biol Interact. 1990;73(2-3):195–205. [PubMed]
  • Fuchs RP. In vitro recognition of carcinogen-induced local denaturation sites native DNA by S1 endonuclease from Aspergillus oryzae. Nature. 1975 Sep 11;257(5522):151–152. [PubMed]
  • Martin-Bertram H. S1-sensitive sites in DNA after gamma-irradiation. Biochim Biophys Acta. 1981 Feb 26;652(2):261–265. [PubMed]
  • Andrews J, Martin-Bertram H, Hagen U. S1 nuclease-sensitive sites in yeast DNA: an assay for radiation-induced base damage. Int J Radiat Biol Relat Stud Phys Chem Med. 1984 May;45(5):497–504. [PubMed]
  • Millican TA, Mock GA, Chauncey MA, Patel TP, Eaton MA, Gunning J, Cutbush SD, Neidle S, Mann J. Synthesis and biophysical studies of short oligodeoxynucleotides with novel modifications: a possible approach to the problem of mixed base oligodeoxynucleotide synthesis. Nucleic Acids Res. 1984 Oct 11;12(19):7435–7453. [PMC free article] [PubMed]
  • Wei CF, Legerski RJ, Alianell GA, Robberson DL, Gray HB., Jr A single apurinic site can elicit BAL 31 nuclease-catalyzed cleavage in duplex DNA. Biochim Biophys Acta. 1984 Sep 10;782(4):408–414. [PubMed]
  • Chaudhry MA, Weinfeld M. The action of Escherichia coli endonuclease III on multiply damaged sites in DNA. J Mol Biol. 1995 Jun 23;249(5):914–922. [PubMed]
  • Wang TS, Korn D. Reactivity of KB cell deoxyribonucleic acid polymerases alpha and beta with nicked and gapped deoxyribonucleic acid. Biochemistry. 1980 Apr 29;19(9):1782–1790. [PubMed]
  • Withka JM, Wilde JA, Bolton PH, Mazumder A, Gerlt JA. Characterization of conformational features of DNA heteroduplexes containing aldehydic abasic sites. Biochemistry. 1991 Oct 15;30(41):9931–9940. [PubMed]
  • Silber JR, Loeb LA. S1 nuclease does not cleave DNA at single-base mis-matches. Biochim Biophys Acta. 1981 Dec 28;656(2):256–264. [PubMed]
  • Shishido K, Ando T. Site-specific fragmentation of bacteriophage T5 DNA by single-strand-specific S1 endonuclease. Biochim Biophys Acta. 1975 Apr 16;390(1):125–132. [PubMed]
  • Wiegand RC, Godson GN, Radding CM. Specificity of the S1 nuclease from Aspergillus oryzae. J Biol Chem. 1975 Nov 25;250(22):8848–8855. [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...