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Nucleic Acids Res. Aug 25, 1995; 23(16): 3343–3344.
PMCID: PMC307197

Low concentrations of tetramethylammonium chloride increase yield and specificity of PCR.

Abstract

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Selected References

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  • Mullis KB, Faloona FA. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 1987;155:335–350. [PubMed]
  • Melchior WB, Jr, Von Hippel PH. Alteration of the relative stability of dA-dT and dG-dC base pairs in DNA. Proc Natl Acad Sci U S A. 1973 Feb;70(2):298–302. [PMC free article] [PubMed]
  • Honoré B, Madsen P, Leffers H. The tetramethylammonium chloride method for screening of cDNA libraries using highly degenerate oligonucleotides obtained by backtranslation of amino-acid sequences. J Biochem Biophys Methods. 1993 Aug;27(1):39–48. [PubMed]
  • Prat A, Katinka M, Caron F, Meyer E. Nucleotide sequence of the Paramecium primaurelia G surface protein. A huge protein with a highly periodic structure. J Mol Biol. 1986 May 5;189(1):47–60. [PubMed]
  • Breslauer KJ, Frank R, Blöcker H, Marky LA. Predicting DNA duplex stability from the base sequence. Proc Natl Acad Sci U S A. 1986 Jun;83(11):3746–3750. [PMC free article] [PubMed]
  • Cummings DJ. Studies on Macronuclear DNA from Paramecium aurelia. Chromosoma. 1975 Dec 10;53(3):191–208. [PubMed]

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