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Nucleic Acids Res. 1995 Mar 25; 23(6): 1087–1088.
PMCID: PMC306810

An improved PCR method for walking in uncloned genomic DNA.


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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ochman H, Gerber AS, Hartl DL. Genetic applications of an inverse polymerase chain reaction. Genetics. 1988 Nov;120(3):621–623. [PMC free article] [PubMed]
  • Parker JD, Rabinovitch PS, Burmer GC. Targeted gene walking polymerase chain reaction. Nucleic Acids Res. 1991 Jun 11;19(11):3055–3060. [PMC free article] [PubMed]
  • Rosenthal A, Jones DS. Genomic walking and sequencing by oligo-cassette mediated polymerase chain reaction. Nucleic Acids Res. 1990 May 25;18(10):3095–3096. [PMC free article] [PubMed]
  • Riley J, Butler R, Ogilvie D, Finniear R, Jenner D, Powell S, Anand R, Smith JC, Markham AF. A novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones. Nucleic Acids Res. 1990 May 25;18(10):2887–2890. [PMC free article] [PubMed]
  • Lagerström M, Parik J, Malmgren H, Stewart J, Pettersson U, Landegren U. Capture PCR: efficient amplification of DNA fragments adjacent to a known sequence in human and YAC DNA. PCR Methods Appl. 1991 Nov;1(2):111–119. [PubMed]
  • Jones DH, Winistorfer SC. Genome walking with 2- to 4-kb steps using panhandle PCR. PCR Methods Appl. 1993 Feb;2(3):197–203. [PubMed]
  • Domínguez O, López-Larrea C. Gene walking by unpredictably primed PCR. Nucleic Acids Res. 1994 Aug 11;22(15):3247–3248. [PMC free article] [PubMed]
  • Iwahana H, Tsujisawa T, Katashima R, Yoshimoto K, Itakura M. PCR with end trimming and cassette ligation: a rapid method to clone exon-intron boundaries and a 5'-upstream sequence of genomic DNA based on a cDNA sequence. PCR Methods Appl. 1994 Aug;4(1):19–25. [PubMed]
  • Barnes WM. PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates. Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2216–2220. [PMC free article] [PubMed]
  • Cheng S, Fockler C, Barnes WM, Higuchi R. Effective amplification of long targets from cloned inserts and human genomic DNA. Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5695–5699. [PMC free article] [PubMed]
  • Degen SJ, Rajput B, Reich E. The human tissue plasminogen activator gene. J Biol Chem. 1986 May 25;261(15):6972–6985. [PubMed]

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