• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of narLink to Publisher's site
Nucleic Acids Res. Jul 24, 1987; 15(14): 5833–5844.
PMCID: PMC306027

The structure of poly(dA):poly(dT) in a condensed state and in solution.


New X-ray and energetically optimal models of poly(dA):poly(dT) with the hydration spine in the minor groove have been compared with the NMR data in solution (Behling, R.W. and Kearns, D.R. (1986) Biochemistry 25, 3335-3346). These models have been refined to achieve a better fit with the NMR data. The obtained results suggest that the poly(dA):poly(dT) structure in a condensed state is similar to that in solution. The proposed conformations of poly(dA):poly(dT), unlike the classic B form, satisfy virtually all geometrical requirements which follow from the NMR data. Thus, the X-ray and energetically optimal poly(dA):poly(dT) structures (or those with slight modifications) can be considered as credible models of the poly(dA):poly(dT) double helix in solution. One of the features distinguishing these models from the classic B form is a narrowed minor groove.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1003K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Koo HS, Wu HM, Crothers DM. DNA bending at adenine . thymine tracts. Nature. 1986 Apr 10;320(6062):501–506. [PubMed]
  • Arnott S, Chandrasekaran R, Hall IH, Puigjaner LC. Heteronomous DNA. Nucleic Acids Res. 1983 Jun 25;11(12):4141–4155. [PMC free article] [PubMed]
  • Alexeev DG, Lipanov AA, Skuratovskii IYa Poly(dA).poly(dT) is a B-type double helix with a distinctively narrow minor groove. Nature. 325(6107):821–823. [PubMed]
  • Fratini AV, Kopka ML, Drew HR, Dickerson RE. Reversible bending and helix geometry in a B-DNA dodecamer: CGCGAATTBrCGCG. J Biol Chem. 1982 Dec 25;257(24):14686–14707. [PubMed]
  • Drew HR, Dickerson RE. Structure of a B-DNA dodecamer. III. Geometry of hydration. J Mol Biol. 1981 Sep 25;151(3):535–556. [PubMed]
  • Kopka ML, Fratini AV, Drew HR, Dickerson RE. Ordered water structure around a B-DNA dodecamer. A quantitative study. J Mol Biol. 1983 Jan 5;163(1):129–146. [PubMed]
  • Chuprina VP. Regularities in formation of the spine of hydration in the DNA minor groove and its influence on the DNA structure. FEBS Lett. 1985 Jul 1;186(1):98–102. [PubMed]
  • Chuprina VP. Anomalous structure and properties of poly (dA).poly(dT). Computer simulation of the polynucleotide structure with the spine of hydration in the minor groove. Nucleic Acids Res. 1987 Jan 12;15(1):293–311. [PMC free article] [PubMed]
  • Peck LJ, Wang JC. Sequence dependence of the helical repeat of DNA in solution. Nature. 1981 Jul 23;292(5821):375–378. [PubMed]
  • Rhodes D, Klug A. Sequence-dependent helical periodicity of DNA. Nature. 1981 Jul 23;292(5821):378–380. [PubMed]
  • Strauss F, Gaillard C, Prunell A. Helical periodicity of DNA, Poly(dA) . poly(dT) and poly(dA-dT). poly(dA-dT) in solution. Eur J Biochem. 1981 Aug;118(2):215–222. [PubMed]
  • Horowitz DS, Wang JC. Torsional rigidity of DNA and length dependence of the free energy of DNA supercoiling. J Mol Biol. 1984 Feb 15;173(1):75–91. [PubMed]
  • Sarma MH, Gupta G, Sarma RH. Untenability of the heteronomous DNA model for poly(dA).poly(dT) in solution. This DNA adopts a right-handed B-DNA duplex in which the two strands are conformationally equivalent. A 500 MHz NMR study using one dimensional NOE. J Biomol Struct Dyn. 1985 Jun;2(6):1057–1084. [PubMed]
  • Behling RW, Kearns DR. 1H two-dimensional nuclear Overhauser effect and relaxation studies of poly(dA).poly(dT) Biochemistry. 1986 Jun 3;25(11):3335–3346. [PubMed]
  • Arnott S, Selsing E. Structures for the polynucleotide complexes poly(dA) with poly (dT) and poly(dT) with poly(dA) with poly (dT). J Mol Biol. 1974 Sep 15;88(2):509–521. [PubMed]
  • Zhurkin VB, Poltev VI, Florent'ev VL. Atom-atomnye potentsial'nye funktsii dlia konformatsionnykh raschetov nukleinovykh kislot. Mol Biol (Mosk) 1980 Sep-Oct;14(5):1116–1130. [PubMed]
  • Poltev VI, Grokhlina TI, Malenkov GG. Hydration of nucleic acid bases studied using novel atom-atom potential functions. J Biomol Struct Dyn. 1984 Oct;2(2):413–429. [PubMed]
  • Ul'ianov NB, Zhurkin VB. Izuchenie gibkosti komplementarnykh dinukleozidfosfatov metodom Monte-Karlo. Mol Biol (Mosk) 1982 Sep-Oct;16(5):1075–1085. [PubMed]
  • Drew HR, Travers AA. DNA structural variations in the E. coli tyrT promoter. Cell. 1984 Jun;37(2):491–502. [PubMed]
  • Behling RW, Kearns DR. Determination of NOE "silent" dipolar interactions between magnetically equivalent nuclei: application to poly(dA).poly(dT). Biopolymers. 1985 Jul;24(7):1157–1167. [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Compound
    PubChem Compound links
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...