• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of narLink to Publisher's site
Nucleic Acids Res. Jul 10, 1987; 15(13): 5443–5459.
PMCID: PMC305971

Human cardiac myosin heavy chain genes and their linkage in the genome.

Abstract

Human myosin heavy chains are encoded by a multigene family consisting of at least 10 members. A gene-specific oligonucleotide has been used to isolate the human beta myosin heavy chain gene from a group of twelve nonoverlapping genomic clones. We have shown that this gene (which is expressed in both cardiac and skeletal muscle) is located 3.6kb upstream of the alpha cardiac myosin gene. We find that DNA sequences located upstream of rat and human alpha cardiac myosin heavy chain genes are very homologous over a 300bp region. Analogous regions of two other myosin genes expressed in different muscles (cardiac and skeletal) show no such homology to each other. While a human skeletal muscle myosin heavy chain gene cluster is located on chromosome 17, we show that the beta and alpha human cardiac myosin heavy chain genes are located on chromosome 14.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.9M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Harrington WF, Rodgers ME. Myosin. Annu Rev Biochem. 1984;53:35–73. [PubMed]
  • Whalen RG, Sell SM, Butler-Browne GS, Schwartz K, Bouveret P, Pinset-Härstöm I. Three myosin heavy-chain isozymes appear sequentially in rat muscle development. Nature. 1981 Aug 27;292(5826):805–809. [PubMed]
  • Whalen RG, Sell SM, Eriksson A, Thornell LE. Myosin subunit types in skeletal and cardiac tissues and their developmental distribution. Dev Biol. 1982 Jun;91(2):478–484. [PubMed]
  • Bárány M. ATPase activity of myosin correlated with speed of muscle shortening. J Gen Physiol. 1967 Jul;50(6 Suppl):197–218. [PMC free article] [PubMed]
  • Geliebter J, Zeff RA, Schulze DH, Pease LR, Weiss EH, Mellor AL, Flavell RA, Nathenson SG. Interaction between Kb and Q4 gene sequences generates the Kbm6 mutation. Mol Cell Biol. 1986 Feb;6(2):645–652. [PMC free article] [PubMed]
  • Weydert A, Daubas P, Lazaridis I, Barton P, Garner I, Leader DP, Bonhomme F, Catalan J, Simon D, Guénet JL, et al. Genes for skeletal muscle myosin heavy chains are clustered and are not located on the same mouse chromosome as a cardiac myosin heavy chain gene. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7183–7187. [PMC free article] [PubMed]
  • Mahdavi V, Periasamy M, Nadal-Ginard B. Molecular characterization of two myosin heavy chain genes expressed in the adult heart. Nature. 1982 Jun 24;297(5868):659–664. [PubMed]
  • Saez L, Leinwand LA. Characterization of diverse forms of myosin heavy chain expressed in adult human skeletal muscle. Nucleic Acids Res. 1986 Apr 11;14(7):2951–2969. [PMC free article] [PubMed]
  • Lompré AM, Nadal-Ginard B, Mahdavi V. Expression of the cardiac ventricular alpha- and beta-myosin heavy chain genes is developmentally and hormonally regulated. J Biol Chem. 1984 May 25;259(10):6437–6446. [PubMed]
  • Hoh JF, McGrath PA, Hale PT. Electrophoretic analysis of multiple forms of rat cardiac myosin: effects of hypophysectomy and thyroxine replacement. J Mol Cell Cardiol. 1978 Nov;10(11):1053–1076. [PubMed]
  • Friedman DJ, Umeda PK, Sinha AM, Hsu HJ, Jakovcic S, Rabinowitz M. Characterization of genomic clones specifying rabbit alpha- and beta-ventricular myosin heavy chains. Proc Natl Acad Sci U S A. 1984 May;81(10):3044–3048. [PMC free article] [PubMed]
  • Mahdavi V, Chambers AP, Nadal-Ginard B. Cardiac alpha- and beta-myosin heavy chain genes are organized in tandem. Proc Natl Acad Sci U S A. 1984 May;81(9):2626–2630. [PMC free article] [PubMed]
  • Izumo S, Nadal-Ginard B, Mahdavi V. All members of the MHC multigene family respond to thyroid hormone in a highly tissue-specific manner. Science. 1986 Feb 7;231(4738):597–600. [PubMed]
  • Sinha AM, Umeda PK, Kavinsky CJ, Rajamanickam C, Hsu HJ, Jakovcic S, Rabinowitz M. Molecular cloning of mRNA sequences for cardiac alpha- and beta-form myosin heavy chains: expression in ventricles of normal, hypothyroid, and thyrotoxic rabbits. Proc Natl Acad Sci U S A. 1982 Oct;79(19):5847–5851. [PMC free article] [PubMed]
  • Dillmann WH. Hormonal influences on cardiac myosin ATPase activity and myosin isoenzyme distribution. Mol Cell Endocrinol. 1984 Mar;34(3):169–181. [PubMed]
  • Everett AW, Sinha AM, Umeda PK, Jakovcic S, Rabinowitz M, Zak R. Regulation of myosin synthesis by thyroid hormone: relative change in the alpha- and beta-myosin heavy chain mRNA levels in rabbit heart. Biochemistry. 1984 Apr 10;23(8):1596–1599. [PubMed]
  • Leinwand LA, Fournier RE, Nadal-Ginard B, Shows TB. Multigene family for sarcomeric myosin heavy chain in mouse and human DNA: localization on a single chromosome. Science. 1983 Aug 19;221(4612):766–769. [PubMed]
  • Benton WD, Davis RW. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Dale RM, McClure BA, Houchins JP. A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA. Plasmid. 1985 Jan;13(1):31–40. [PubMed]
  • Messing J, Crea R, Seeburg PH. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. [PMC free article] [PubMed]
  • Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. [PubMed]
  • Shows TB, Sakaguchi AY, Naylor SL. Mapping the human genome, cloned genes, DNA polymorphisms, and inherited disease. Adv Hum Genet. 1982;12:341–452. [PubMed]
  • Shows TB, Sakaguchi AY, Naylor SL, Goedell DV, Lawn RM. Clustering of leukocyte and fibroblast interferon genes of human chromosome 9. Science. 1982 Oct 22;218(4570):373–374. [PubMed]
  • Wilbur WJ, Lipman DJ. Rapid similarity searches of nucleic acid and protein data banks. Proc Natl Acad Sci U S A. 1983 Feb;80(3):726–730. [PMC free article] [PubMed]
  • Gulick J, Kropp K, Robbins J. The structure of two fast-white myosin heavy chain promoters. A comparative study. J Biol Chem. 1985 Nov 25;260(27):14513–14520. [PubMed]
  • Wydro RM, Nguyen HT, Gubits RM, Nadal-Ginard B. Characterization of sarcomeric myosin heavy chain genes. J Biol Chem. 1983 Jan 10;258(1):670–678. [PubMed]
  • Lichter P, Umeda PK, Levin JE, Vosberg HP. Partial characterization of the human beta-myosin heavy-chain gene which is expressed in heart and skeletal muscle. Eur J Biochem. 1986 Oct 15;160(2):419–426. [PubMed]
  • Dean DC, Knoll BJ, Riser ME, O'Malley BW. A 5'-flanking sequence essential for progesterone regulation of an ovalbumin fusion gene. Nature. 1983 Oct 6;305(5934):551–554. [PubMed]
  • Robins DM, Paek I, Seeburg PH, Axel R. Regulated expression of human growth hormone genes in mouse cells. Cell. 1982 Jun;29(2):623–631. [PubMed]
  • Gunning P, Ponte P, Kedes L, Eddy R, Shows T. Chromosomal location of the co-expressed human skeletal and cardiac actin genes. Proc Natl Acad Sci U S A. 1984 Mar;81(6):1813–1817. [PMC free article] [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Gene
    Gene
    Gene links
  • Gene (nucleotide)
    Gene (nucleotide)
    Records in Gene identified from shared sequence links
  • GEO Profiles
    GEO Profiles
    Related GEO records
  • HomoloGene
    HomoloGene
    HomoloGene links
  • MedGen
    MedGen
    Related information in MedGen
  • Nucleotide
    Nucleotide
    Published Nucleotide sequences
  • OMIM
    OMIM
    OMIM record citing PubMed
  • Protein
    Protein
    Published protein sequences
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...