Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. 1987 May; 84(9): 2732–2736.
PMCID: PMC304732

A chemical model for the cooperation of sulfates and carboxylates in calcite crystal nucleation: Relevance to biomineralization

Abstract

Acidic matrix macromolecules involved in regulation of biological crystal growth often contain aspartic acid-rich domains and covalently bound sulfated polysaccharides. We propose that sulfates and β-sheet structured carboxylates cooperate in oriented calcite crystal nucleation. The sulfates concentrate calcium, creating the supersaturation necessary for nucleation on the structured carboxylate domains. An artificial model, composed of sulfonated polystyrene surfaces and adsorbed β-sheet poly(aspartate), demonstrates that the two components indeed act cooperatively with respect to two independent assays, both by induction of calcite nucleation off the (001) plane and by calcium association. Evidence is presented that a purified organic matrix acidic glycoprotein from mollusk shells may behave in vitro in a similar way.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Weiner S. Organization of extracellularly mineralized tissues: a comparative study of biological crystal growth. CRC Crit Rev Biochem. 1986;20(4):365–408. [PubMed]
  • Addadi L, Weiner S. Interactions between acidic proteins and crystals: stereochemical requirements in biomineralization. Proc Natl Acad Sci U S A. 1985 Jun;82(12):4110–4114. [PMC free article] [PubMed]
  • Chen CC, Boskey AL. Mechanisms of proteoglycan inhibition of hydroxyapatite growth. Calcif Tissue Int. 1985 Jul;37(4):395–400. [PubMed]
  • Thiele H, Awad A. Nucleation and oriented crystallization apatite in ionotropic gels. J Biomed Mater Res. 1969 Sep;3(3):431–441. [PubMed]
  • Maroudas NG. Sulphonated polystyrene as an optimal substratum for the adhesion and spreading of mesenchymal cells in monovalent and divalent saline solutions. J Cell Physiol. 1977 Mar;90(3):511–519. [PubMed]
  • Hoare DG, Koshland DE., Jr A method for the quantitative modification and estimation of carboxylic acid groups in proteins. J Biol Chem. 1967 May 25;242(10):2447–2453. [PubMed]
  • Nagasawa K, Inoue Y, Kamata T. Solvolytic desulfation of glycosaminoglycuronan sulfates with dimethyl sulfoxide containing water or methanol. Carbohydr Res. 1977 Sep;58(1):47–55. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Tsay TG, Veis A. Preparation, detection, and characterization of an antibody to rat alpha-phosphophoryn. Biochemistry. 1985 Nov 5;24(23):6363–6369. [PubMed]
  • Greenfield N, Fasman GD. Computed circular dichroism spectra for the evaluation of protein conformation. Biochemistry. 1969 Oct;8(10):4108–4116. [PubMed]
  • Simkiss K. The organic matrix of the oyster shell. Comp Biochem Physiol. 1965 Dec;16(4):427–435. [PubMed]
  • Fisher LW, Termine JD, Dejter SW, Jr, Whitson SW, Yanagishita M, Kimura JH, Hascall VC, Kleinman HK, Hassell JR, Nilsson B. Proteoglycans of developing bone. J Biol Chem. 1983 May 25;258(10):6588–6594. [PubMed]
  • Torchia DA, Hasson MA, Hascall VC. Investigation of molecular motion of proteoglycans in cartilage by 13C magnetic resonance. J Biol Chem. 1977 Jun 10;252(11):3617–3625. [PubMed]
  • Lee SL, Veis A. Cooperativity in calcium ion binding to repetitive, carboxylate-serylphosphate polypeptides and the relationship of this property to dentin mineralization. Int J Pept Protein Res. 1980 Sep;16(3):231–240. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • PubMed
    PubMed
    PubMed citations for these articles

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...