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Nature. Author manuscript; available in PMC Jul 27, 2011.
Published in final edited form as:
PMCID: PMC3031026
EMSID: UKMS33223

Distinct physiological and behavioural functions for parental alleles of imprinted Grb10

Abstract

Imprinted genes, defined by their preferential expression of a single parental allele, represent a subset of the mammalian genome and often have key roles in embryonic development1, but also post-natal functions including energy homeostasis2 and behaviour3, 4. When the two parental alleles are unequally represented within a social group (when there is sex-bias in dispersal and/or variance in reproductive success)5, 6, imprinted genes may evolve to modulate social behaviour, although to date no such instance is known. Predominantly expressed from the maternal allele during embryogenesis, Grb10 encodes an intracellular adapter protein that can interact with a number of receptor tyrosine kinases and downstream signalling molecules7. Here we demonstrate that within the brain Grb10 is expressed from the paternal allele from fetal life into adulthood and that ablation of this expression engenders increased social dominance specifically among other aspects of social behaviour, a finding supported by the observed increase in allogrooming by paternal Grb10 deficient animals. Grb10 is, therefore, the first example of an imprinted gene that regulates social behaviour. It is also currently alone in exhibiting imprinted expression from each of the parental alleles in a tissue specific manner, as loss of the peripherally expressed maternal allele leads to significant fetal and placental overgrowth. Thus, Grb10 is to date a unique imprinted gene, able to influence distinct physiological processes, fetal growth and adult behaviour, due to actions of the two parental alleles in different tissues.

To characterise expression and investigate functions of the two parental Grb10 alleles we have generated a mutant mouse strain (Grb10KO), derived by insertion of a LacZ:neomycinr gene-trap cassette within Grb10 exon 8 (Figure 1a). Transmission of the Grb10KO allele separately through the two parental lines generated heterozygous progeny in which either the maternal (Grb10KOm/+) or paternal (Grb10KO+/p) Grb10 allele was disrupted by the β-geo cassette and allowed us to examine Grb10 expression in an allele-specific manner. Northern blot analysis of RNA samples prepared from whole fetuses (Figure 1b) showed that endogenous Grb10 transcripts were readily detected in wild type animals and in heterozygotes that inherited a mutant Grb10KO+/p allele. In contrast, Grb10 transcripts were found at relatively low levels in heterozygous animals with a mutant Grb10KOm/+ allele, an observation consistent with previous demonstrations that the majority of Grb10 expression is maternally derived (e.g. Ref 8). We next conducted more refined in situ analyses of allele-specific expression, utilising the integrated LacZ reporter gene. During fetal development, LacZ expression from the maternal allele was widespread in tissues of mesodermal and endodermal origin, but absent from the central nervous system (CNS) proper (Figure 1d, f). At e14.5 expression of the maternal Grb10 allele within the brain was seen only in the ventricular ependymal layers, the epithelium of the choroid plexus and the meninges, presumably identifying sources of maternal brain expression that have been reported by others9-13 (Supplemental Figures 1a, b). In contrast, expression from the paternal allele was predominant within the developing CNS, with only a few discrete sites of relatively low level expression seen in other tissues (Figures 1e, g, ,2a2a and Supplemental Figures 1c, d). The CNS expression commences between e11.5 and e14.5, consistent with the onset of neurogenesis, and correlates with the brain-specific loss of a repressive histone modification (H3K27me3) from the paternal Grb10 allele during development and during neural precursor cell differentiation in vitro14. This loss of H3K27me3 from the promoter region of the Grb10 paternal allele-specific transcripts (see Figure 1a) leaves a permissive histone mark on the paternal allele (H3K4me2), whereas this region of the maternal allele is constitutively associated with two repressive histone modifications (H3K9me3 and H4K20me3)14.

Figure 1
Generation and characterisation of Grb10KO mutants
Figure 2
Grb10 expression in the mouse brain

Our analysis showed paternal allele expression within the developing CNS was restricted to specific regions of both the brain and spinal cord, with reporter signal identified within select areas of the diencephalon, ventral midbrain and the medulla oblongata extending caudally along the ventral spinal cord. There was no expression detected within the presumptive neocortex, dorsal midbrain or the cerebellar primordium (Figure 2a). Embryonic Grb10 expression within the CNS proper was entirely paternal in origin, a fact that was not evident from previous expression studies that identified a promoter and brain-specific transcripts associated with the paternal allele, but relied on techniques involving RNA extraction from tissue homogenates9-12. Thus, our Grb10 expression analysis provides striking evidence of reciprocal imprinted expression from the two parental alleles in different tissues. A number of imprinted genes exhibit tissue specific and/or temporal regulation, such that their expression is biallelic (non-imprinted) at some of their sites of expression. However, the reciprocal parent-of-origin expression described here is unprecedented, suggesting new and intriguing possibilities for imprinted gene function and evolution.

Consistent with our previous studies of Grb10Δ2-4 mice8, 15, Grb10KOm/+ animals displayed a disproportionate overgrowth phenotype apparent from e12.5 onwards (Figure 1h,i and Supplementary Figure 2). At birth, the mean body weight of Grb10KOm/+ pups was 25±2.5% greater than that of wild type littermates. The liver was disproportionately enlarged (117±9.8% heavier), but there was sparing of the brain and kidney, such that the weights of these organs were not significantly different to those of wild types (Figure 1i). The cranial sparing is consistent with limited Grb10 maternal allele expression within the developing CNS. Body weight and proportions of Grb10KO+/p mutants did not differ from wild type controls and no function has yet been ascribed to the paternally inherited Grb10 allele, despite evidence of its expression within the neonatal brain9. Both Grb10KOm/+ and Grb10KO+/p mutants were present at the expected Mendelian frequencies (χ2 values, p=0.737 and p=0.395, respectively) when animals were genotyped at 3-4 weeks of age, indicating that survival to weaning was unimpaired. Observations of Grb10KO+/p pups prior to weaning, including analysis of stomach weights (Figure 1j), suggested that suckling behaviour was normal.

Grb10 expression in the adult brain was consistent with that observed during embryogenesis in being predominately paternally derived. Northern blot analysis demonstrated the presence of Grb10 transcripts in the wild type and Grb10KOm/+ brains but not in the Grb10KO+/p brain (Figure 1c), with no effects observed on expression of the adjacent Ddc gene (data not shown). Consistent with this, expression of the LacZ reporter was derived exclusively from the Grb10KO+/p allele (Figure 2a-d and Supplemental Figure 3). Histological analysis of LacZ expression in the adult Grb10KO+/p brain revealed a discrete pattern of paternal allele expression (Figure 2k-m). Reporter expression was observed within thalamic, hypothalamic, midbrain and hindbrain nuclei, with no cortical expression detected at any point throughout the brain. Forebrain expression was also evident within the septal nuclei and specifically the cholinergic inter-neurones of the caudate putamen. Within the midbrain and hindbrain sites of expression included almost all monoaminergic cell populations (for a complete list of sites of paternal allele expression see Supplementary Table 1). In situ hybridisation analysis of endogenous Grb10 mRNA expression was in close accordance with the observed LacZ expression profile, indicating that it was not an artefact of reporter insertion (Figure 2e-j and Supplemental Figure 4). Grb10 maternal allele expression is dramatically downregulated from late gestation and persists post-natally in only a subset of peripheral tissues16. Consistent with this, ependymal and choroid plexus epithelial expression observed in the embryo was no longer apparent in the Grb10KOm/+ adult. In situ hybridisation analysis of Grb10KO+/p brains revealed an almost complete absence of maternal Grb10 expression. A low level of maternal allele expression was detected in a small number of brain regions, including, the median preoptic nucleus, medial habenular, medial amygdaloid nuclei and ventromedial hypothalamus (Supplemental Figure 4), therefore representing sites of biallelic Grb10 expression. These sites within the brain mirror the situation outside the CNS where expression from the maternal allele predominates, but there are also discrete sites of biallelic expression (Figure 1e,g). Expression from the paternal allele in the CNS followed a pattern suggesting that expression established during embryogenesis was maintained into adulthood, as demonstrated by analysis of endogenous Grb10 expression in Grb10KOm/+ brains (Supplemental Figure 5).

The distribution of LacZ positive cells within expressing regions of the adult brain suggested expression from the paternal allele was predominantly neuron-specific. Supporting this, immunofluorescent colocalisation experiments carried out within three discrete brain regions, substantia nigra pars compacta, dorsal raphe nucleus and caudate putamen, demonstrated that paternal Grb10 expression was detectable in dopaminergic (Figure 2n), serotonergic (Figure 2o) and cholinergic (Figure 2p) neurons, respectively.

Despite having established a role for maternal Grb10 as a major regulator of both fetal and placental growth8, 15 (Figure 1 and Supplementary Figure 2), we found no evidence of brain overgrowth in neonatal Grb10KO+/p mutant animals (Figure 1i). A number of genes show imprinted expression in the brain and knockout mouse studies have shown that some of these genes regulate specific behaviours. Notably, these include paternally expressed genes important for maternal nurturing of young (Mest and Peg3, reviewed in Ref 4), but also genes regulating other behavioural functions, including exploratory behaviour (maternally expressed Nesp55; 17) and circadian rhythm output (paternally expressed Magel2; 18). We therefore sought to assay Grb10KO+/p mutant animals using standard tests of different behavioural parameters. In most of these assays Grb10KO+/p mice were essentially indistinguishable from wild type littermate controls, including tests of anxiety-related behaviour, locomotor activity, olfaction and aggression (Supplementary Figure 5). However, in an assay of social dominance in which a forced encounter was observed between two unfamiliar animals, using the tube-test paradigm19, Grb10KO+/p mutants were found to be significantly less likely to back down than their wild-type “opponents” (Figure 3a). This was not the case for Grb10KOm/+ mice (Supplemental Figure 5i). In the context of all of our behavioural testing, the outcome of the tube-test was interpreted as a specific change in the behaviour of Grb10KO+/p mutant animals. Moreover, this behavioural change was found to correlate with observations made of socially housed mice, where there was a significantly elevated incidence of facial barbering in cages containing at least one Grb10KO+/p mutant (Figure 3b, c). Typically, these cages contained a single unbarbered Grb10KO+/p mutant (81% of cages), suggesting this animal was responsible for allogrooming of cage-mates. Consistent with this, isolation of barbered animals facilitated complete regrowth of missing hair and vibrissae. Barbering was observed in both male and female cages (Supplemental Figure 5j). Allogrooming is regarded to be a robust correlate of social dominance, as its assessment is independent of exogenous confounds20. Rigorous testing of additional aspects of social behaviour in Grb10KO+/p mice revealed no further differences in comparison with wild type littermate controls (Fig 3d, e). Specifically, habituation-dishabituation studies designed to probe aspects of social recognition, pertinent to the interpretation of the tube-test data, indicated that Grb10KO+/p animals reacted normally by exhibiting a general habituation to the olfactory cue, urine, followed by subsequent dishabituation when presented with a novel odour. Consistent with this outcome, Grb10KO+/p mutant mice exhibited normal olfactory responses when tested in their latency to investigate two different odours (Supplemental Fig 4e, f).

Figure 3
Increased social dominance in Grb10KO+/p mice

Our study identifies Grb10 as the first imprinted gene to have a role in the modulation of a specific social behaviour (as distinct from parental care). This function is predicted to be subject to the effects of intra-genomic conflict within social groups when the two parental alleles are unequally represented, notably when there is sex-bias in dispersal and/or variance in reproductive success5, 6. In mice, as in other mammals6 there is probably greater variance in reproductive success in males than in females and unequal representation is thus very likely. However, whether the association of the phenotypes with the sex-of-origin that we observe are consistent with the theory is unclear. For species such as humans, in which there is greater variation between males in reproductive success and (most probably) female dispersal, the involvement of paternally derived genes in promoting more altruistic behaviours is expected6. For mice, however, the necessary parameters are not well enough described to enable confident prediction as to whether paternal or maternal alleles should be the more “altruistic”6. We note that our finding might also be considered consistent with the co-adaptation theory of imprinted gene evolution21, 22. Similarly, the effect of Grb10 on placental growth8, 15 is potentially consistent both with the parental conflict and co-adaptation theories.

It has also been suggested that differences in parental genome representation within social groups could engender differential tolerance to risk-taking behaviours4. Tempering of socially dominant behaviour can be viewed as a risk-averse phenotype aimed at maximising reproductive success by avoiding the potentially detrimental consequences of challenging for social status. Expression of Grb10 within a number of monoaminergic nuclei may be relevant to the possible underlying mechanism, as cerebrospinal fluid levels of serotonin and dopamine metabolites have been independently correlated with dominant/submissive behaviour23, 24. However, no changes in the levels of dopamine, serotonin, noradrenalin and acetylcholine (and associated metabolites) were detected from macro-dissected brain regions of Grb10KO+/p mice (Supplemental Figure 6). The imprinted Nesp55 gene has been associated with the promotion of risk-tolerance, notable because Nesp55 is expressed from the maternal allele within discrete brain regions that overlap sites of Grb10 paternal allele expression, including the serotonergic raphe nucleus and noradrenergic neurons of the locus coeruleus4, 17. This raises the possibility that these two genes might represent antagonistic components within the same neurological systems. Moreover, a recent genome-wide screen has indicated that over 1300 loci could be subject to parent-of-origin allelic expression bias within the mouse brain13, suggesting the influence of genomic imprinting within the brain may be much greater than previously thought, although verification of this will require extensive validation of allelic expression bias together with functional testing of the identified genes.

Many imprinted genes are found in clusters that can contain genes expressed from either parental allele as well as non-imprinted genes25. However, the demonstration of opposite imprinting within a single mouse gene, most likely conserved in humans26, represents a highly provocative situation, whereby the two parental alleles of Grb10 have evolved distinct patterns of imprinted expression according to their functions in different tissues.

Methods Summary

Grb10KO mice

Chimeric animals were generated by microinjection of a gene-trap ES cell line (XC302; Baygenomics, California, USA) into F2 (C57BL/6 × CBA) strain blastocysts, using standard methods27. Mice were maintained on a C57BL/6:CBA mixed genetic background and kept as previously described28. Behavioural phenotyping and statistical methods are detailed in the online Methods section.

Northern blot analysis

Total RNA was extracted using TRI reagent (Sigma Aldrich), with 20-50 μg run on denaturing agarose gels and transferred to a nylon membrane for hybridisation with a Grb10 specific radiolabelled probe8.

In situ hybridisation

Adult brain tissue was collected from animals transcardially perfused with 4% paraformaldehyde, cryoprotected in 20% sucrose and sectioned at 30 μm on a freezing microtome. Tissue was processed for in situ hybridisation29 and a [35S] radiolabelled riboprobe specific to exons 11-16 of the mouse Grb10 mRNA sequence was used to detect endogenous Grb10 expression.

LacZ expression analysis

Dissected embryos were fixed in 2% formaldehyde/0.2% glutaraldehyde in PBS for 2 hours at room temperature, stained at 37°C for approximately 2 hours in freshly prepared X-gal solution, post-fixed overnight at 4°C using 4% paraformaldehyde in PBS, then cleared in 80% glycerol. Adult brains were longitudinally bisected and stained, as above, without fixation. For adult brain sections, animals were first perfused with chilled 9.25% (w/v) sucrose solution, followed by approximately 100 ml of chilled 3% paraformaldehyde in 0.1% PBS. Brains were sectioned at 50 μm on a vibratome (VT1000S; Leica), with the tissue kept ice cold. Free-floating sections were collected and immersed in X-gal staining solution at 28°C overnight. Grb10KO samples were coetaneously stained alongside wild type controls.

Immunofluorescent analysis of adult brain sections

50 μm brain sections were collected from animals perfused with 4% paraformaldehyde and the sections post-fixed in 4% paraformaldehyde at 4°C overnight prior to antibody staining, as described30.

Full Methods and any associated references are available in the online version of this paper at www.nature.com/nature.

Supplementary Material

Acknowledgements

We thank Sue Wonnacott for reagents, the University of Bath Biological Services Unit and Steve Routley for technical assistance, Ian Jones and Paul Mitchell for invaluable advice, and Cheryll Tickle for comments on the manuscript. We gratefully acknowledge funding of the work from the BBSRC, Medical Research Council, Wellcome Trust and external benefactors.

A.W. and A.S.G. conceived the project and interpreted the data, with input from L.D.H., A.R.I. and L.S.W.; K.M and J.E.S-C generated the Grb10KO mice; A.S.G carried out the bulk of the experiments with contributions from M.C, J.W.D., S.B., K.G., A.R.I., F.M.S, J.X. and A.W; A.S.G and A.W jointly wrote the manuscript.

Footnotes

References

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