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Infect Immun. Aug 1994; 62(8): 3550–3553.
PMCID: PMC302991

The virulence gene cluster of Listeria monocytogenes is also present in Listeria ivanovii, an animal pathogen, and Listeria seeligeri, a nonpathogenic species.

Abstract

Most known Listeria monocytogenes virulence genes cluster within a 9.6-kb chromosomal region. This region is flanked on one end by two uncharacterized open reading frames (ORF A and ORF B) and ldh, an ORF presumably encoding the L. monocytogenes lactate dehydrogenase (J.-A. Vazquez-Boland, C. Kocks, S. Dramsi, H. Ohayon, C. Geoffroy, J. Mengaud, and P. Cossart, Infect. Immun. 60:219-230, 1992). We report here that the other end is flanked by prs, and ORF homologous to phosphoribosyl PPi synthetase genes. ORF B and prs were detected in all Listeria species and thus delimit the virulence region. This virulence gene cluster was detected exclusively in hemolytic Listeria species, Listeria ivanovii, an animal pathogen, and Listeria seeligeri, a nonpathogenic species.

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Selected References

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  • Bower SG, Hove-Jensen B, Switzer RL. Structure of the gene encoding phosphoribosylpyrophosphate synthetase (prsA) in Salmonella typhimurium. J Bacteriol. 1988 Jul;170(7):3243–3248. [PMC free article] [PubMed]
  • Camilli A, Goldfine H, Portnoy DA. Listeria monocytogenes mutants lacking phosphatidylinositol-specific phospholipase C are avirulent. J Exp Med. 1991 Mar 1;173(3):751–754. [PMC free article] [PubMed]
  • Camilli A, Tilney LG, Portnoy DA. Dual roles of plcA in Listeria monocytogenes pathogenesis. Mol Microbiol. 1993 Apr;8(1):143–157. [PubMed]
  • Chakraborty T, Leimeister-Wächter M, Domann E, Hartl M, Goebel W, Nichterlein T, Notermans S. Coordinate regulation of virulence genes in Listeria monocytogenes requires the product of the prfA gene. J Bacteriol. 1992 Jan;174(2):568–574. [PMC free article] [PubMed]
  • Cooper RF, Dennis SM. Further characterization of Listeria monocytogenes serotype 5. Can J Microbiol. 1978 May;24(5):598–599. [PubMed]
  • Cossart P, Mengaud J. Listeria monocytogenes. A model system for the molecular study of intracellular parasitism. Mol Biol Med. 1989 Oct;6(5):463–474. [PubMed]
  • Domann E, Leimeister-Wächter M, Goebel W, Chakraborty T. Molecular cloning, sequencing, and identification of a metalloprotease gene from Listeria monocytogenes that is species specific and physically linked to the listeriolysin gene. Infect Immun. 1991 Jan;59(1):65–72. [PMC free article] [PubMed]
  • Domann E, Wehland J, Rohde M, Pistor S, Hartl M, Goebel W, Leimeister-Wächter M, Wuenscher M, Chakraborty T. A novel bacterial virulence gene in Listeria monocytogenes required for host cell microfilament interaction with homology to the proline-rich region of vinculin. EMBO J. 1992 May;11(5):1981–1990. [PMC free article] [PubMed]
  • Dramsi S, Kocks C, Forestier C, Cossart P. Internalin-mediated invasion of epithelial cells by Listeria monocytogenes is regulated by the bacterial growth state, temperature and the pleiotropic activator prfA. Mol Microbiol. 1993 Sep;9(5):931–941. [PubMed]
  • Farber JM, Peterkin PI. Listeria monocytogenes, a food-borne pathogen. Microbiol Rev. 1991 Sep;55(3):476–511. [PMC free article] [PubMed]
  • Gaillard JL, Berche P, Frehel C, Gouin E, Cossart P. Entry of L. monocytogenes into cells is mediated by internalin, a repeat protein reminiscent of surface antigens from gram-positive cocci. Cell. 1991 Jun 28;65(7):1127–1141. [PubMed]
  • Geoffroy C, Gaillard JL, Alouf JE, Berche P. Production of thiol-dependent haemolysins by Listeria monocytogenes and related species. J Gen Microbiol. 1989 Mar;135(3):481–487. [PubMed]
  • Geoffroy C, Raveneau J, Beretti JL, Lecroisey A, Vazquez-Boland JA, Alouf JE, Berche P. Purification and characterization of an extracellular 29-kilodalton phospholipase C from Listeria monocytogenes. Infect Immun. 1991 Jul;59(7):2382–2388. [PMC free article] [PubMed]
  • Gormley E, Mengaud J, Cossart P. Sequences homologous to the listeriolysin O gene region of Listeria monocytogenes are present in virulent and avirulent haemolytic species of the genus Listeria. Res Microbiol. 1989 Nov-Dec;140(9):631–643. [PubMed]
  • Coune A. Liposomes as drug delivery system in the treatment of infectious diseases. Potential applications and clinical experience. Infection. 1988 May-Jun;16(3):141–147. [PubMed]
  • Hove-Jensen B. Mutation in the phosphoribosylpyrophosphate synthetase gene (prs) that results in simultaneous requirements for purine and pyrimidine nucleosides, nicotinamide nucleotide, histidine, and tryptophan in Escherichia coli. J Bacteriol. 1988 Mar;170(3):1148–1152. [PMC free article] [PubMed]
  • Hove-Jensen B, Harlow KW, King CJ, Switzer RL. Phosphoribosylpyrophosphate synthetase of Escherichia coli. Properties of the purified enzyme and primary structure of the prs gene. J Biol Chem. 1986 May 25;261(15):6765–6771. [PubMed]
  • Iizasa T, Taira M, Shimada H, Ishijima S, Tatibana M. Molecular cloning and sequencing of human cDNA for phosphoribosyl pyrophosphate synthetase subunit II. FEBS Lett. 1989 Feb 13;244(1):47–50. [PubMed]
  • Karunasagar I, Krohne G, Goebel W. Listeria ivanovii is capable of cell-to-cell spread involving actin polymerization. Infect Immun. 1993 Jan;61(1):162–169. [PMC free article] [PubMed]
  • Kocks C, Gouin E, Tabouret M, Berche P, Ohayon H, Cossart P. L. monocytogenes-induced actin assembly requires the actA gene product, a surface protein. Cell. 1992 Feb 7;68(3):521–531. [PubMed]
  • Köhler S, Leimeister-Wächter M, Chakraborty T, Lottspeich F, Goebel W. The gene coding for protein p60 of Listeria monocytogenes and its use as a specific probe for Listeria monocytogenes. Infect Immun. 1990 Jun;58(6):1943–1950. [PMC free article] [PubMed]
  • Kreft J, Funke D, Haas A, Lottspeich F, Goebel W. Production, purification and characterization of hemolysins from Listeria ivanovii and Listeria monocytogenes Sv4b. FEMS Microbiol Lett. 1989 Jan 15;48(2):197–202. [PubMed]
  • Leimeister-Wächter M, Domann E, Chakraborty T. Detection of a gene encoding a phosphatidylinositol-specific phospholipase C that is co-ordinately expressed with listeriolysin in Listeria monocytogenes. Mol Microbiol. 1991 Feb;5(2):361–366. [PubMed]
  • Leimeister-Wächter M, Haffner C, Domann E, Goebel W, Chakraborty T. Identification of a gene that positively regulates expression of listeriolysin, the major virulence factor of listeria monocytogenes. Proc Natl Acad Sci U S A. 1990 Nov;87(21):8336–8340. [PMC free article] [PubMed]
  • Mengaud J, Braun-Breton C, Cossart P. Identification of phosphatidylinositol-specific phospholipase C activity in Listeria monocytogenes: a novel type of virulence factor? Mol Microbiol. 1991 Feb;5(2):367–372. [PubMed]
  • Mengaud J, Dramsi S, Gouin E, Vazquez-Boland JA, Milon G, Cossart P. Pleiotropic control of Listeria monocytogenes virulence factors by a gene that is autoregulated. Mol Microbiol. 1991 Sep;5(9):2273–2283. [PubMed]
  • Mengaud J, Geoffroy C, Cossart P. Identification of a new operon involved in Listeria monocytogenes virulence: its first gene encodes a protein homologous to bacterial metalloproteases. Infect Immun. 1991 Mar;59(3):1043–1049. [PMC free article] [PubMed]
  • Mengaud J, Vicente MF, Cossart P. Transcriptional mapping and nucleotide sequence of the Listeria monocytogenes hlyA region reveal structural features that may be involved in regulation. Infect Immun. 1989 Dec;57(12):3695–3701. [PMC free article] [PubMed]
  • Michel E, Cossart P. Physical map of the Listeria monocytogenes chromosome. J Bacteriol. 1992 Nov;174(22):7098–7103. [PMC free article] [PubMed]
  • Nilsson D, Hove-Jensen B, Arnvig K. Primary structure of the tms and prs genes of Bacillus subtilis. Mol Gen Genet. 1989 Sep;218(3):565–571. [PubMed]
  • Notermans SH, Dufrenne J, Leimeister-Wächter M, Domann E, Chakraborty T. Phosphatidylinositol-specific phospholipase C activity as a marker to distinguish between pathogenic and nonpathogenic Listeria species. Appl Environ Microbiol. 1991 Sep;57(9):2666–2670. [PMC free article] [PubMed]
  • Portnoy DA, Chakraborty T, Goebel W, Cossart P. Molecular determinants of Listeria monocytogenes pathogenesis. Infect Immun. 1992 Apr;60(4):1263–1267. [PMC free article] [PubMed]
  • Taira M, Ishijima S, Kita K, Yamada K, Iizasa T, Tatibana M. Nucleotide and deduced amino acid sequences of two distinct cDNAs for rat phosphoribosylpyrophosphate synthetase. J Biol Chem. 1987 Nov 5;262(31):14867–14870. [PubMed]
  • Tilney LG, Tilney MS. The wily ways of a parasite: induction of actin assembly by Listeria. Trends Microbiol. 1993 Apr;1(1):25–31. [PubMed]
  • Vazquez-Boland JA, Dominguez L, Rodriguez-Ferri EF, Suarez G. Purification and characterization of two Listeria ivanovii cytolysins, a sphingomyelinase C and a thiol-activated toxin (ivanolysin O). Infect Immun. 1989 Dec;57(12):3928–3935. [PMC free article] [PubMed]
  • Vazquez-Boland JA, Kocks C, Dramsi S, Ohayon H, Geoffroy C, Mengaud J, Cossart P. Nucleotide sequence of the lecithinase operon of Listeria monocytogenes and possible role of lecithinase in cell-to-cell spread. Infect Immun. 1992 Jan;60(1):219–230. [PMC free article] [PubMed]
  • Walker JE, Gay NJ, Saraste M, Eberle AN. DNA sequence around the Escherichia coli unc operon. Completion of the sequence of a 17 kilobase segment containing asnA, oriC, unc, glmS and phoS. Biochem J. 1984 Dec 15;224(3):799–815. [PMC free article] [PubMed]

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