• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
Infect Immun. Aug 1994; 62(8): 3479–3484.
PMCID: PMC302981

Cellular responses to a 55-kilodalton recombinant Pneumocystis carinii antigen.

Abstract

The host-parasite interaction in Pneumocystis carinii pneumonia is poorly understood. In recent years, two major groups of P. carinii antigens have been identified. One class of antigens is characterized by a broad band of immunoreactivity between 45 and 55 kDa in P. carinii derived from rats. This antigen complex is the P. carinii antigen most commonly found in respiratory tract specimens and most frequently recognized by the host immune response. The availability of a recombinant antigen has permitted studies focusing on the cellular and humoral responses to a single antigen within this class, p55. In this study, we have demonstrated that the p55 antigen elicits a cell-mediated immune response in animals previously exposed to P. carinii. Under conditions of natural exposure, the 5' portion of the molecule, p55(1-200), appears immunologically silent, failing to elicit lymphocyte proliferation or cytokine secretion. Following active immunization, the 5' portion is capable of stimulating lymphocyte proliferation. The 3' portion, p55(268-414), has at least one immunodominant region which contains a 7-amino-acid repeat motif. The cells responding to p55 include a CD4+ T cell which secretes a Th1 cytokine pattern. A detailed understanding of the host-parasite interaction will facilitate the development of immunoprophylaxis and immunotherapy for P. carinii infection.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Anders RF. Multiple cross-reactivities amongst antigens of Plasmodium falciparum impair the development of protective immunity against malaria. Parasite Immunol. 1986 Nov;8(6):529–539. [PubMed]
  • Beck JM, Warnock ML, Curtis JL, Sniezek MJ, Arraj-Peffer SM, Kaltreider HB, Shellito JE. Inflammatory responses to Pneumocystis carinii in mice selectively depleted of helper T lymphocytes. Am J Respir Cell Mol Biol. 1991 Aug;5(2):186–197. [PubMed]
  • Boom WH, Wallis RS, Chervenak KA. Human Mycobacterium tuberculosis-reactive CD4+ T-cell clones: heterogeneity in antigen recognition, cytokine production, and cytotoxicity for mononuclear phagocytes. Infect Immun. 1991 Aug;59(8):2737–2743. [PMC free article] [PubMed]
  • Chen W, Havell EA, Harmsen AG. Importance of endogenous tumor necrosis factor alpha and gamma interferon in host resistance against Pneumocystis carinii infection. Infect Immun. 1992 Apr;60(4):1279–1284. [PMC free article] [PubMed]
  • Chen W, Havell EA, Moldawer LL, McIntyre KW, Chizzonite RA, Harmsen AG. Interleukin 1: an important mediator of host resistance against Pneumocystis carinii. J Exp Med. 1992 Sep 1;176(3):713–718. [PMC free article] [PubMed]
  • Fisher DJ, Gigliotti F, Zauderer M, Harmsen AG. Specific T-cell response to a Pneumocystis carinii surface glycoprotein (gp120) after immunization and natural infection. Infect Immun. 1991 Oct;59(10):3372–3376. [PMC free article] [PubMed]
  • Forte M, Maartens G, Campbell F, Stubberfield C, Shahmanesh M, Kumararatne D, Gaston H. T-lymphocyte responses to Pneumocystis carinii in healthy and HIV-positive individuals. J Acquir Immune Defic Syndr. 1992;5(4):409–416. [PubMed]
  • Gigliotti F. Host species-specific antigenic variation of a mannosylated surface glycoprotein of Pneumocystis carinii. J Infect Dis. 1992 Feb;165(2):329–336. [PubMed]
  • Gigliotti F, Ballou LR, Hughes WT, Mosley BD. Purification and initial characterization of a ferret Pneumocystis carinii surface antigen. J Infect Dis. 1988 Oct;158(4):848–854. [PubMed]
  • Glimcher LH, Schroer JA, Chan C, Shevach EM. Fine specificity of cloned insulin-specific T cell hybridomas: evidence supporting a role for tertiary conformation. J Immunol. 1983 Dec;131(6):2868–2874. [PubMed]
  • Graves DC, McNabb SJ, Worley MA, Downs TD, Ivey MH. Analyses of rat Pneumocystis carinii antigens recognized by human and rat antibodies by using western immunoblotting. Infect Immun. 1986 Oct;54(1):96–103. [PMC free article] [PubMed]
  • Hagler DN, Deepe GS, Pogue CL, Walzer PD. Blastogenic responses to Pneumocystis carinii among patients with human immunodeficiency (HIV) infection. Clin Exp Immunol. 1988 Oct;74(1):7–13. [PMC free article] [PubMed]
  • Haidaris PJ, Wright TW, Gigliotti F, Haidaris CG. Expression and characterization of a cDNA clone encoding an immunodominant surface glycoprotein of Pneumocystis carinii. J Infect Dis. 1992 Nov;166(5):1113–1123. [PubMed]
  • Harmsen AG, Chen W. Resolution of Pneumocystis carinii pneumonia in CD4+ lymphocyte-depleted mice given aerosols of heat-treated Escherichia coli. J Exp Med. 1992 Sep 1;176(3):881–886. [PMC free article] [PubMed]
  • Hoffman OA, Standing JE, Limper AH. Pneumocystis carinii stimulates tumor necrosis factor-alpha release from alveolar macrophages through a beta-glucan-mediated mechanism. J Immunol. 1993 May 1;150(9):3932–3940. [PubMed]
  • Kolls JK, Beck JM, Nelson S, Summer WR, Shellito J. Alveolar macrophage release of tumor necrosis factor during murine Pneumocystis carinii pneumonia. Am J Respir Cell Mol Biol. 1993 Apr;8(4):370–376. [PubMed]
  • Kovacs JA, Halpern JL, Lundgren B, Swan JC, Parrillo JE, Masur H. Monoclonal antibodies to Pneumocystis carinii: identification of specific antigens and characterization of antigenic differences between rat and human isolates. J Infect Dis. 1989 Jan;159(1):60–70. [PubMed]
  • Kovacs JA, Halpern JL, Swan JC, Moss J, Parrillo JE, Masur H. Identification of antigens and antibodies specific for Pneumocystis carinii. J Immunol. 1988 Mar 15;140(6):2023–2031. [PubMed]
  • Kovacs JA, Powell F, Edman JC, Lundgren B, Martinez A, Drew B, Angus CW. Multiple genes encode the major surface glycoprotein of Pneumocystis carinii. J Biol Chem. 1993 Mar 15;268(8):6034–6040. [PubMed]
  • Krishnan VL, Meager A, Mitchell DM, Pinching AJ. Alveolar macrophages in AIDS patients: increased spontaneous tumour necrosis factor-alpha production in Pneumocystis carinii pneumonia. Clin Exp Immunol. 1990 May;80(2):156–160. [PMC free article] [PubMed]
  • Limper AH, Pottratz ST, Martin WJ., 2nd Modulation of Pneumocystis carinii adherence to cultured lung cells by a mannose-dependent mechanism. J Lab Clin Med. 1991 Nov;118(5):492–499. [PubMed]
  • Linke MJ, Cushion MT, Walzer PD. Properties of the major antigens of rat and human Pneumocystis carinii. Infect Immun. 1989 May;57(5):1547–1555. [PMC free article] [PubMed]
  • Lundgren B, Lipschik GY, Kovacs JA. Purification and characterization of a major human Pneumocystis carinii surface antigen. J Clin Invest. 1991 Jan;87(1):163–170. [PMC free article] [PubMed]
  • Murray HW. Interferon-gamma, the activated macrophage, and host defense against microbial challenge. Ann Intern Med. 1988 Apr;108(4):595–608. [PubMed]
  • Pottratz ST, Martin WJ., 2nd Mechanism of Pneumocystis carinii attachment to cultured rat alveolar macrophages. J Clin Invest. 1990 Nov;86(5):1678–1683. [PMC free article] [PubMed]
  • Pottratz ST, Martin WJ., 2nd Role of fibronectin in Pneumocystis carinii attachment to cultured lung cells. J Clin Invest. 1990 Feb;85(2):351–356. [PMC free article] [PubMed]
  • Romani L, Mocci S, Bietta C, Lanfaloni L, Puccetti P, Bistoni F. Th1 and Th2 cytokine secretion patterns in murine candidiasis: association of Th1 responses with acquired resistance. Infect Immun. 1991 Dec;59(12):4647–4654. [PMC free article] [PubMed]
  • Roths JB, Sidman CL. Both immunity and hyperresponsiveness to Pneumocystis carinii result from transfer of CD4+ but not CD8+ T cells into severe combined immunodeficiency mice. J Clin Invest. 1992 Aug;90(2):673–678. [PMC free article] [PubMed]
  • Ruoslahti E, Pierschbacher MD. Arg-Gly-Asp: a versatile cell recognition signal. Cell. 1986 Feb 28;44(4):517–518. [PubMed]
  • Schleifer KW, Filutowicz H, Schopf LR, Mansfield JM. Characterization of T helper cell responses to the trypanosome variant surface glycoprotein. J Immunol. 1993 Apr 1;150(7):2910–2919. [PubMed]
  • Smulian AG, Stringer JR, Linke MJ, Walzer PD. Isolation and characterization of a recombinant antigen of Pneumocystis carinii. Infect Immun. 1992 Mar;60(3):907–915. [PMC free article] [PubMed]
  • Smulian AG, Sullivan DW, Linke MJ, Halsey NA, Quinn TC, MacPhail AP, Hernandez-Avila MA, Hong ST, Walzer PD. Geographic variation in the humoral response to Pneumocystis carinii. J Infect Dis. 1993 May;167(5):1243–1247. [PubMed]
  • Smulian AG, Theus SA, Denko N, Walzer PD, Stringer JR. A 55 kDa antigen of Pneumocystis carinii: analysis of the cellular immune response and characterization of the gene. Mol Microbiol. 1993 Mar;7(5):745–753. [PubMed]
  • Stringer SL, Garbe T, Sunkin SM, Stringer JR. Genes encoding antigenic surface glycoproteins in Pneumocystis from humans. J Eukaryot Microbiol. 1993 Nov-Dec;40(6):821–826. [PubMed]
  • Tamburrini E, De Luca A, Ventura G, Maiuro G, Siracusano A, Ortona E, Antinori A. Pneumocystis carinii stimulates in vitro production of tumor necrosis factor-alpha by human macrophages. Med Microbiol Immunol. 1991;180(1):15–20. [PubMed]
  • Theus SA, Linke MJ, Andrews RP, Walzer PD. Proliferative and cytokine responses to a major surface glycoprotein of Pneumocystis carinii. Infect Immun. 1993 Nov;61(11):4703–4709. [PMC free article] [PubMed]
  • Ullmann A. One-step purification of hybrid proteins which have beta-galactosidase activity. Gene. 1984 Jul-Aug;29(1-2):27–31. [PubMed]
  • Wada M, Kitada K, Saito M, Egawa K, Nakamura Y. cDNA sequence diversity and genomic clusters of major surface glycoprotein genes of Pneumocystis carinii. J Infect Dis. 1993 Oct;168(4):979–985. [PubMed]
  • Walzer PD, Linke MJ. A comparison of the antigenic characteristics of rat and human Pneumocystis carinii by immunoblotting. J Immunol. 1987 Apr 1;138(7):2257–2265. [PubMed]
  • Willadsen P, Eisemann CH, Tellam RL. 'Concealed' antigens: expanding the range of immunological targets. Parasitol Today. 1993 Apr;9(4):132–135. [PubMed]
  • Willadsen P, McKenna RV. Vaccination with 'concealed' antigens: myth or reality? Parasite Immunol. 1991 Nov;13(6):605–616. [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...