Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
Infect Immun. Aug 1994; 62(8): 3222–3229.
PMCID: PMC302949

Mycobacterium marinum persists in cultured mammalian cells in a temperature-restricted fashion.

Abstract

We have explored the relatively rapidly growing animal and human pathogen Mycobacterium marinum as an experimental model for mycobacterial pathogenesis. M. marinum, which has a lower temperature for optimal growth than does Mycobacterium tuberculosis, has a much shorter generation time and can be safely studied in ordinary laboratory facilities and examined in multiple animal infection models. We have established an in vitro assay for its interaction with eukaryotic cells and shown that it persists in these cells in a temperature-specific fashion that correlates with its ability to cause disease in vivo at lower temperatures. Additionally, preliminary evidence that M. marinum causes a chronic disease with some features resembling tuberculosis in frogs of the species Rana pipiens is presented.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (3.5M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Arruda S, Bomfim G, Knights R, Huima-Byron T, Riley LW. Cloning of an M. tuberculosis DNA fragment associated with entry and survival inside cells. Science. 1993 Sep 10;261(5127):1454–1457. [PubMed]
  • Bloom BR, Murray CJ. Tuberculosis: commentary on a reemergent killer. Science. 1992 Aug 21;257(5073):1055–1064. [PubMed]
  • CLARK HF, SHEPARD CC. EFFECT OF ENVIRONMENTAL TEMPERATURES ON INFECTION WITH MYCOBACTERIUM MARINUM (BALNEI) OF MICE AND A NUMBER OF POIKILOTHERMIC SPECIES. J Bacteriol. 1963 Nov;86:1057–1069. [PMC free article] [PubMed]
  • Collins FM, Montalbine V, Morrison NE. Growth and immunogenicity of photochromogenic strains of mycobacteria in the footpads of normal mice. Infect Immun. 1975 May;11(5):1079–1087. [PMC free article] [PubMed]
  • Francis CL, Ryan TA, Jones BD, Smith SJ, Falkow S. Ruffles induced by Salmonella and other stimuli direct macropinocytosis of bacteria. Nature. 1993 Aug 12;364(6438):639–642. [PubMed]
  • Gadea I, Zapardiel J, Ruiz P, Gegúndez MI, Esteban J, Soriano F. Cytopathic effect mimicking virus culture due to Mycobacterium tuberculosis. J Clin Microbiol. 1993 Sep;31(9):2517–2518. [PMC free article] [PubMed]
  • Gray SF, Smith RS, Reynolds NJ, Williams EW. Fish tank granuloma. BMJ. 1990 Apr 21;300(6731):1069–1070. [PMC free article] [PubMed]
  • Huminer D, Pitlik SD, Block C, Kaufman L, Amit S, Rosenfeld JB. Aquarium-borne Mycobacterium marinum skin infection. Report of a case and review of the literature. Arch Dermatol. 1986 Jun;122(6):698–703. [PubMed]
  • Jacobs WR, Jr, Kalpana GV, Cirillo JD, Pascopella L, Snapper SB, Udani RA, Jones W, Barletta RG, Bloom BR. Genetic systems for mycobacteria. Methods Enzymol. 1991;204:537–555. [PubMed]
  • Joiner KA, Fuhrman SA, Miettinen HM, Kasper LH, Mellman I. Toxoplasma gondii: fusion competence of parasitophorous vacuoles in Fc receptor-transfected fibroblasts. Science. 1990 Aug 10;249(4969):641–646. [PubMed]
  • King CH, Mundayoor S, Crawford JT, Shinnick TM. Expression of contact-dependent cytolytic activity by Mycobacterium tuberculosis and isolation of the genomic locus that encodes the activity. Infect Immun. 1993 Jun;61(6):2708–2712. [PMC free article] [PubMed]
  • Lambertus MW, Mathisen GE. Mycobacterium marinum infection in a patient with cryptosporidiosis and the acquired immunodeficiency syndrome. Cutis. 1988 Jul;42(1):38–40. [PubMed]
  • LINELL F, NORDEN A. Mycobacterium balnei, a new acid-fast bacillus occurring in swimming pools and capable of producing skin lesions in humans. Acta Tuberc Scand Suppl. 1954;33:1–84. [PubMed]
  • McDonough KA, Kress Y, Bloom BR. Pathogenesis of tuberculosis: interaction of Mycobacterium tuberculosis with macrophages. Infect Immun. 1993 Jul;61(7):2763–2773. [PMC free article] [PubMed]
  • Ries KM, White GL, Jr, Murdock RT. Atypical mycobacterial infection caused by Mycobacterium marinum. N Engl J Med. 1990 Mar 1;322(9):633–633. [PubMed]
  • Rogall T, Wolters J, Flohr T, Böttger EC. Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium. Int J Syst Bacteriol. 1990 Oct;40(4):323–330. [PubMed]
  • SHEPARD CC. Growth characteristics of tubercle bacilli and certain other mycobacteria in HeLa cells. J Exp Med. 1957 Jan 1;105(1):39–48. [PMC free article] [PubMed]
  • SHEPARD CC. Growth characteristics in HeLa cells of the rapidly growing acid fast bacteria, Mycobacterium fortuitum, Mycobacterium phlei, and Mycobacterium smegmatis. J Bacteriol. 1957 Jun;73(6):722–726. [PMC free article] [PubMed]
  • SHEPARD CC. A comparison of the growth of selected mycobacteria in HeLa, monkey kidney, and human amnion cells in tissue culture. J Exp Med. 1958 Feb 1;107(2):237–246. [PMC free article] [PubMed]
  • Swartz RP, Naai D, Vogel CW, Yeager H., Jr Differences in uptake of mycobacteria by human monocytes: a role for complement. Infect Immun. 1988 Sep;56(9):2223–2227. [PMC free article] [PubMed]
  • Tchornobay AM, Claudy AL, Perrot JL, Lévigne V, Denis M. Fatal disseminated Mycobacterium marinum infection. Int J Dermatol. 1992 Apr;31(4):286–287. [PubMed]
  • Weiss R. On the track of "killer" TB. Science. 1992 Jan 10;255(5041):148–150. [PubMed]
  • Woods GL, Washington JA., 2nd Mycobacteria other than Mycobacterium tuberculosis: review of microbiologic and clinical aspects. Rev Infect Dis. 1987 Mar-Apr;9(2):275–294. [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...