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Logo of viroljBioMed CentralBiomed Central Web Sitesearchsubmit a manuscriptregisterthis articleVirology JournalJournal Front Page
Virol J. 2010; 7: 355.
Published online Dec 3, 2010. doi:  10.1186/1743-422X-7-355
PMCID: PMC3004832

Morphogenesis of the T4 tail and tail fibers

Abstract

Remarkable progress has been made during the past ten years in elucidating the structure of the bacteriophage T4 tail by a combination of three-dimensional image reconstruction from electron micrographs and X-ray crystallography of the components. Partial and complete structures of nine out of twenty tail structural proteins have been determined by X-ray crystallography and have been fitted into the 3D-reconstituted structure of the "extended" tail. The 3D structure of the "contracted" tail was also determined and interpreted in terms of component proteins. Given the pseudo-atomic tail structures both before and after contraction, it is now possible to understand the gross conformational change of the baseplate in terms of the change in the relative positions of the subunit proteins. These studies have explained how the conformational change of the baseplate and contraction of the tail are related to the tail's host cell recognition and membrane penetration function. On the other hand, the baseplate assembly process has been recently reexamined in detail in a precise system involving recombinant proteins (unlike the earlier studies with phage mutants). These experiments showed that the sequential association of the subunits of the baseplate wedge is based on the induced-fit upon association of each subunit. It was also found that, upon association of gp53 (gene product 53), the penultimate subunit of the wedge, six of the wedge intermediates spontaneously associate to form a baseplate-like structure in the absence of the central hub. Structure determination of the rest of the subunits and intermediate complexes and the assembly of the hub still require further study.

Introduction

The structures of bacteriophages are unique among viruses in that most of them have tails, the specialized host cell attachment organelles. Phages that possess a tail are collectively called "Caudovirales" [1]. The family Caudovirales is divided into three sub-families according to the tail morphology: Myoviridae (long contractile tail), Siphoviridae (long non-contractile tail), and Podoviridae (short non-contractile tail). Of these, Myoviridae phages have the most complex tail structures with the greatest number of proteins involved in the tail assembly and function. Bacteriophage T4 belongs to this sub-family and has a very high efficiency of infection, likely due to its complex tails and two sets of host-cell binding fibers (Figure (Figure1).1). In laboratory conditions, virtually every phage particle can adsorb onto a bacterium and is successful in injecting the DNA into the cytosol [2].

Figure 1
Structure of bacteriophage T4. (A) Schematic representation; CryoEM-derived model of the phage particle prior to (B) and upon (C) host cell attachment. Tail fibers are disordered in the cryoEM structures, as they represent the average of many particles ...

Since the emergence of conditional lethal mutants in the 1960's [3], assembly of the phage as well as its molecular genetics have been extensively studied as reviewed in "Molecular biology of bacteriophage T4" [4]. During the past ten years, remarkable progress has been made in understanding the conformational transformation of the tail baseplate from a "hexagon" to a "star" shape, which occurs upon attachment of the phage to the host cell surface. Three-dimensional image reconstructions have been determined of the baseplate, both before [5] and after [6] tail contraction using cryo-electron microscopy and complete or partial atomic structures of eight out of 15 baseplate proteins have been solved [7-14]. The atomic structures of these proteins were fitted into the reconstructions [15]. The fact that the crystal structures of the constituent proteins could be unambiguously placed in both conformations of the baseplate indicated that the gross conformational change of the baseplate is caused by a rearrangement or relative movement of the subunit proteins, rather than associated with large structural changes of individual proteins. This has now provided a good understanding of the mechanics of the structural transformation of the baseplate, which will be discussed in this review.

Assembly Pathway of the Tail

The tail of bacteriophage T4 is a very large macromolecular complex, comprised of about 430 polypeptide chains with a molecular weight of approximately 2 × 107 (Tables (Tables1,1, ,22 and and3).3). Twenty two genes are involved in the assembly of the T4 tail (Tables (Tables1,1, ,22 and and3).3). The tail consists of a sheath, an internal tail tube and a baseplate, situated at the distal end of the tail. Two types of fibers (the long tail fibers and the short tail fibers), responsible for host cell recognition and binding, are attached to the baseplate.

Table 1
Tail proteins listed in the order of assembly into the complete tail 172425.
Table 2
Chaperones involved in the assembly of the tail, tail fibers and attachment of the fibers to the phage particle 7172343446274.
Table 3
T4 fibers 17186265.

The assembly pathway of the T4 tail has been extensively studied by a number of authors and has been reviewed earlier [16-20]. The main part of the assembly pathway has been elucidated by Kikuchi and King [21-23] with the help of elaborate complementation assays and electron microscopy. The lysates of various amber mutant phage-infected cells were fractionated on sucrose density gradients and complemented with each other in vitro. The assembly pathway is strictly ordered and consists of many steps (Figure (Figure2).2). If one of the gene products is missing, the assembly proceeds to the point where the missing product would be required, leaving the remaining gene products in an "assembly naïve" soluble form, as is especially apparent in the baseplate wedge assembly. The assembly pathway has been confirmed by in vivo assembly experiments by Ferguson and Coombs (Table (Table1)1) [24] who performed pulse-chase experiments using 35S-labeled methionine and monitored the accumulation of the labeled gene products in the completed tail. They confirmed the previously proposed assembly pathway and showed that the order of appearance of the labeled gene products also depended on the pool size or the existing number of the protein in the cell. The tail genes are 'late' genes that are expressed almost simultaneously at 8 to 10 min after the infection, indicating that the order of the assembly is determined by the protein interactions, but not by the order of expression.

Figure 2
Assembly of the tail. Rows A, B and C show the assembly of the wedge; the baseplate and the tail tube with the sheath, respectively.

The fully assembled baseplate is a prerequisite for the assembly of the tail tube and the sheath both of which polymerize into the extended structure using the baseplate as the assembly nucleus (Figure (Figure2).2). The baseplate is comprised of about 140 polypeptide chains of at least 16 proteins. Two gene products, gp51 and gp57A, are required for assembly, but are not present in the final particle. The baseplate has sixfold symmetry and is assembled from 6 wedges and the central hub. The only known enzyme associated with the phage particle, the T4 tail lysozyme, is a baseplate component. It is encoded by gene 5 (gp5).

The assembly of the wedge, consisting of seven gene products (gp11, gp10, gp7, gp8, gp6, gp53 and gp25), is strictly ordered. When one of the gene products is missing, the intermediate complex before the missing gene product is formed and the remaining gene products stay in a free form in solution. Gp11 is an exception, which can bind to gp10 at any step of the assembly. Recently, all the intermediate complexes and the complete wedge as well as all the individual gene products of the wedge were isolated, and the interactions among the gene products were examined [25]. An unexpected finding was that gp6, gp53 and gp25 interact with each other weakly. Gp53, however, binds strongly to the precursor wedge complex only after gp6 has bound. Similarly, gp53 is required for gp25 binding. These findings strongly indicated that the strict sequential order of the wedge assembly is due to a conformational change of the intermediate complex, which results in the creation of a new binding site rather than formation of a new binding site at the interface between the newly bound gene product and the precursor complex. Another unexpected finding was that the wedge precursor complexes spontaneously assemble into sixfold symmetrical star-shaped baseplate-like, 43S structure as soon as gp53 binds. The 43S baseplate decreases its sedimentation coefficient to 40S after gp25 and gp11 binding, apparently due to a structural change in the baseplate [21-23]. Based on these findings, Yap et al. [25] have postulated that the 40S star-shaped particle is capable of binding the hub and the six short, gp12 tail fibers, to form the 70S dome-shaped baseplate, found in the extended tail.

Several groups studied the assembly and composition of the central part of the baseplate - the hub - and arrived at different, rather contradictory, conclusions [17]. The assembly of the hub is complicated by a branching pathway and by the presence of gp51, an essential protein of unknown function [26]. Structural studies suggest that the hub consists of at least four proteins: gp5, gp27, gp29 and another unidentified small protein, possibly, gp28 [5]. Recent genetic studies support some of the earlier findings that the hub contains gp26 and gp28 [27].

After the formation of the 70S dome-shaped baseplate containing the short tail fibers, six gp9 trimers (the "socket proteins" of the long tail fibers) bind to the baseplate. Gp48 and gp54 bind to the 'upper' part of the baseplate dome to form the platform for polymerization of gp19 for formation of the tube.

The detailed mechanism of the length determination of the tube is unknown, but the strongest current hypothesis suggests that gp29 is incorporated into the baseplate in an unfolded form. Gp29, the "tape-measure protein", extends as more and more copies of the tail tube protomer, gp19, are added to the growing tube[28]. At the end of the tube, the capping protein, gp3, binds to the last row of gp19 subunits (and, possibly, to gp29) to stabilize them. The tail sheath is built from gp18 subunits simultaneously as the tube, using the tube as a scaffold. When the sheath reaches the length of the tube, the tail terminator protein, gp15, binds to gp3 and the last row of gp18 subunits, completing the tail, which becomes competent for attachment to the head. Both gp15 and gp3 form hexameric rings [29].

The assembly pathway of the tail is a component of Movie 1 (http://www.seyet.com/t4_virology.html), which describes the assembly of the entire phage particle.

Tail Structure

Structure of the baseplate and its constituent proteins

The tail consists of the sheath, the internal tail tube and the baseplate, situated at the distal end of the tail (Figures (Figures11 and and2).2). During attachment to the host cell surface, the tail undergoes a large conformational change: The baseplate opens up like a flower, the sheath contracts, and the internal tube is pushed through the baseplate, penetrating the host envelope. The phage DNA is then released into the host cell cytoplasm through the tube. The tail can, therefore, be compared to a syringe, which is powered by the extended spring, the sheath, making the term "macromolecular nanomachine" appropriate.

The baseplate conformation is coupled to that of the sheath: the "hexagonal" conformation is associated with the extended sheath, whereas the "star" conformation is associated with the contracted sheath that occurs in the T4 particle after attachment to the host cell. Before discussing more fully the baseplate and tail structures in their two conformations, the crystal structures of the baseplate constituent proteins as well as relevant biochemical and genetic data will be described.

Crystal structure of the cell-puncturing device, the gp5-gp27 complex

Gp5 was identified as the tail-associated lysozyme, required during infection but not for cell lysis [30]. The lysozyme domain of gp5 is the middle part of the gp5 polypeptide [31]. It has 43% sequence identity to the cytoplasmic T4 lysozyme, encoded by gene e and called T4L [32]. Gp5 was found to undergo post-translational proteolysis [31], which was believed to be required for activation. Kanamaru et al. [33] showed that the C-terminal domain of gp5, which they named gp5C, is a structural component of the phage particle. Furthermore, Kanamaru et al. [33] reported that 1) gp5C is an SDS- and urea-resistant trimer; 2) gp5C is responsible for trimerization of the entire gp5; 3) gp5C is rich in β-structure; 4) post-translational proteolysis occurs between Ser351 and Ala352; 5) gp5C dissociates from the N-terminal part, called gp5*, at elevated temperatures; and that 6) the lysozyme activity of the trimeric gp5 in the presence of gp5C is only 10% of that of the monomeric gp5*. The amino acid sequence of gp5C contains eleven VXGXXXXX repeats. Subsequent studies showed that gp5 forms a stable complex with gp27 in equimolar quantities and that this complex falls apart in low pH conditions (Figure (Figure3).3). Upon cleavage of gp5, this complex consists of 9 polypeptide chains, represented as (gp27-gp5*-gp5C)3.

Figure 3
Assembly of (gp27-gp5*-gp5C)3; reprinted from [13]. A, Domain organization of gp5. The maturation cleavage is indicated with the dotted line. Initial and final residue numbers are shown for each domain. B, Alignment of the octapeptide units composing ...

The crystal structure of the gp5-gp27 complex was determined to a resolution of 2.9 Å [13]. The structure resembles a 190 Å long torch (or flashlight) (Figure (Figure4)4) with the gp27 trimer forming the cylindrical "head" part of the structure. This hollow cylinder has internal and external diameters of about 30 Å and 80 Å, respectively, and is about 60 Å long. The cylinder encompasses three N-terminal domains of the trimeric gp5* to which the 'handle' of the torch is attached. The 'handle' is formed by three intertwined polypeptide chains constituting the gp5 C-terminal domain folded into a trimeric β-helix. The three gp5 lysozyme domains are adjacent to the β-helix. Two long peptide linkers run along the side of the β-helix, connecting the lysozyme domain with the gp5 N- and C-terminal domains. The linker joining the lysozyme domain to the β-helix contains the cleavage site between gp5* and gp5C.

Figure 4
Structure of the gp5-gp27 complex. A, The gp5-gp27 trimer is shown as a ribbon diagram in which each chain is shown in a different color. B, Domains of gp27. The two homologous domains are colored in light green and cyan. C, Side and end on views of the ...

Two domains of gp27 (residues 2 to 111 and residues 207-239 plus 307-368) are homologous (Figure (Figure4).4). They have similar seven- or eight-stranded, antiparallel β-barrel structures, which can be superimposed on each other with the root mean square deviation (RMSD) of 2.4 Å between the 63 equivalent Cα atoms, representing 82% of all Cα atoms. The superposition transformation involves an approximately 60° rotation about the crystallographic threefold axis. Thus, these domains of gp27 form a pseudo-sixfold-symmetric torus in the trimer, which serves as the symmetry adjuster between the trimeric gp5-gp27 complex and the sixfold-symmetric baseplate. Notwithstanding the structural similarity of these two domains, there is only 4% sequence identity of the structurally equivalent amino acids in these two domains. Nevertheless, the electrostatic charge distribution and hydrophilic properties of the gp27 trimer are roughly sixfold symmetric.

Gp5* consists of the N-terminal OB-fold domain and the lysozyme domain. The OB-fold domain is a five-stranded antiparallel β-barrel with a Greek-key topology that was originally observed as being an oligosaccharide/oligonucleotide-binding domain [34]. It is clear now that this fold shows considerable variability of its binding specificity, although the substrate binding site location on the surfaces on most OB-folds has a common site [35]. It is unlikely that the gp5 N-terminal domain is involved in polysaccharide binding, as it lacks the polar residues required for binding sugars. Most probably, the OB-fold has adapted to serve as an adapter between the gp27 trimer and the C-terminal β-helical domain.

The structure of the gp5 lysozyme domain is similar to that of hen egg white lysozyme (HEWL) and T4L having 43% sequence identity with the latter. The two T4 lysozyme structures can be superimposed with an RSMD of 1.1 Å using all Cα atoms in the alignment. There are two small additional loops in gp5, constituting a total of 5 extra residues (Val211-Arg212 and Asn232-Pro233,-Gly234). The active site residues of HEWL, T4L and gp5 are conserved. The known catalytic residues of T4L, Glu11, Asp20, and Thr26, correspond to Glu184, Asp193, and Thr199 in gp5, respectively, establishing that the enzymatic mechanism is the same and that the gp5 lysozyme domain, T4L and HEWL have a common evolutionary origin.

By comparing the crystal structure of T4L with bound substrate [36] to gp5, the inhibition of gp5 lysozyme activity in the presence of the C-terminal β-helix can be explained. Both gp5 and T4L have the same natural substrate, namely E. coli periplasmic cell wall, the major component of which ((NAG-NAM)-LAla-DisoGlu-DAP-DAla [36] ) contains sugar and peptide moieties. In the gp5 trimer, the linker connecting the lysozyme domain to the β-helix prevents binding of the peptide portion of the substrate to the lysozyme domain. At the same time, the polysaccharide binding cleft is sterically blocked by the gp5 β-helix. Dissociation of the β-helix removes both of these blockages and restores the full lysozyme activity of gp5*.

Gp5C, the C-terminal domain of gp5, is a triple-stranded β-helix (Figure (Figure4).4). Three polypeptide chains wind around each other to create an equilateral triangular prism, which is 110 Å long and 28 Å in diameter. Each face has a slight left-handed twist (about 3° per β-strand), as is normally observed in β-sheets. The width of the prism face tapers gradually from 33 Å at the amino end to 25 Å at the carboxy end of the β-helix, thus creating a pointed needle. This narrowing is caused by a decrease in size of the external side chains and by the internal methionines 554 and 557, which break the octapeptide repeat near the tip of the helix. The first 5 β-strands (residues 389-435) form an antiparallel β-sheet, which forms one of the three faces of the prism. The succeeding 18 β-strands comprise a 3-start intertwined β-helix together with the other two, threefold-related polypeptides. The intertwined C-terminal part of the β-helical prism (residues 436-575) is a remarkably smooth continuation of its three non-intertwined N-terminal parts (residues 389-435).

The octapeptide sequence of the helical intertwined part of the prism (residues a through h) has dominant glycines at position a, asparagines or aspartic acids at position b, valines at position g, and polar or charged residues at position h. Residues b through g form extended β-strands (Ramachandran angles [var phi] ≈ -129°, ψ ≈ 128°) that run at an angle of 75° with respect to the helix axis. The glycines at position a ([var phi] = -85°, ψ = -143°, an allowed region of the Ramachandran diagram) and residues at position h ([var phi] = -70°, ψ = -30°, typical for α-helices) kink the polypeptide chain by about 130° clockwise. The conserved valines at position g always point to the inside of the β-helix and form a "knob-into-holes" arrangement with the main chain atoms of the glycines at position a and the aliphatic part of the side chains of residues at position c. Asp436 replaces the normal glycine in position a and is at the start of the β-helix. This substitution may be required for folding of the β-helix, because the Asp436 Oδ atom makes a hydrogen bond with Oγ of Ser427 from the threefold-related polypeptide chain. The side chain oxygen atoms of Asp468, which also occupies position a, forms hydrogen bonds with residues in the lysozyme domain.

The interior of the β-helix is progressively more hydrophobic toward its C-terminal tip. The middle part of the helix has a pore, which is filled with water molecules bound to polar and charged side chains. The helix is stabilized by two ions situated on its symmetry axis: an anion (possibly, a phosphate) coordinated by three Lys454 residues and a hydrated Ca2+ cation (S. Buth, S. Budko, P. Leiman unpublished data) coordinated by three Glu552 residues. These features contribute to the chemical stability of the β-helix, which is resistant to 10% SDS and 2 M guanidine HCl. The surface of the β-helix is highly negatively charged. This charge may be necessary to repel the phosphates of the lipid bilayer when the β-helix penetrates through the outer cell membrane during infection.

Crystal structures of gp6, gp8, gp9, gp10, gp11 and gp12

Genes of all the T4 baseplate proteins were cloned into high level expression vectors individually and in various combinations. Proteins comprising the periphery of the baseplate showed better solubility and could be purified in amounts sufficient for crystallization. The activity was checked in complementation assays using a corresponding amber mutant phage. It was possible to crystallize and solve structures of the full-length gp8, gp9 and gp11 (Figure (Figure5)5) [8-10]. The putative domain organization of gp10 was derived from the cryoEM map of the baseplate. This information was used to design a deletion mutant constituting the C-terminal domain, which was then crystallized [11]. A stable deletion mutant of gp6 suitable for crystallization was identified using limited proteolysis (Figure (Figure5)5) [7]. Full-length gp12 showed a very high tendency to aggregation. Gp12 was subjected to limited proteolysis in various buffers and conditions. Two slightly different proteolysis products, which resulted from these experiments, were crystallized (Figure (Figure5)5) [12,14]. Due to crystal disorder, it was possible to build an atomic model for less than half of the crystallized gp12 fragments [12,14].

Figure 5
Crystal structures of the baseplate proteins. The star (*) symbol after the protein name denotes that the crystal structure is available for the C-terminal fragment of the protein. Residue numbers comprising the solved structure are given in parentheses. ...

Two proteins, gp6 and gp8, are dimers, whereas the rest of the crystallized proteins - gp9, gp10, gp11 and gp12 - are trimers. None of the proteins had a structural homolog in the Protein Data Bank when these structures were determined. Neither previous studies nor new structural information suggested any enzymatic activity for these proteins. The overall fold of gp12 is the most remarkable of the six mentioned proteins. The topology of the C-terminal globular part is so complex that it creates an impression that the three polypeptide chains knot around each other [14]. This is not the case, however, because the polypeptide chains can be pulled apart from their ends without entanglement. Thus the fold has been characterized as being 'knitted', but not 'knotted' [14]. Gp12 was reported to be a Zn-containing protein [37] and X-ray fluorescent data supported this finding, although Zn was present in the purification buffer [14]. The Zn atom was found to be buried deep inside the C-terminal domain. It is positioned on the threefold axis of the protein and is coordinated by the side chains of His445 and His447 from each of the three chains, resulting in octahedral geometry that is unusual for Zn [12,14,38].

Although gp12, like gp5, contains a triple-stranded β-helix (Figure (Figure5)5) these helices are quite different in their structural and biochemical properties. The gp12 β-helix is narrower than the gp5 β-helix because there are 6 residues (on average) per turn in the gp12 β-helix compared to 8 in gp5. The interior of the gp12 β-helix is hydrophobic, whereas only the interior of the C-terminal tip of the gp5 β-helix is hydrophobic, but the rest is quite hydrophilic, contains water, phosphate and lipid molecules (S. Buth, S. Budko, P. Leiman unpublished data). Furthermore, the gp12 β-helix lacks the well defined gp5-like repeat.

Many functional analogs of the T4 short tail fibers in other bacteriophages have enzymatic activity and are called tailspikes. The endosialidase from phage K1F and its close homologs from phages K1E, K1-5 and CUS3 contain a very similar β-helix that has several small loops, which create a secondary substrate-binding site [39-41]. The gp12-like β-helix can be found in tail fibers of many lactophages [42], and is a very common motif for proteins that participate in lipopolysaccharide (LPS) binding. However, most gp12-like β-helices do not possess LPS binding sites. Furthermore, unlike gp5, the gp12-like β-helix cannot fold on its own, requiring a chaperone, (e.g. T4 gp57A) for folding correctly [43,44]. Nevertheless, gp12-like β-helix might have enough flexibility and possesses other properties that render give it LPS binding proteins.

The T4 baseplate is significantly more complex than that of phage P2 or Mu, two other well studied contractile tail phages [45,46], and contains at least five extra proteins (gp7, gp8, gp9, gp10 and gp11), all positioned at the baseplate's periphery. T4 gp25 and gp6 have genes W and J as homologs in P2, respectively ([45] and P. Leiman unpublished data). However, the origin and evolutionary relationships for the rest of the baseplate proteins cannot be detected at the amino acid level. The crystal structure of the C-terminal fragment (residues 397 - 602) of gp10 has provided some clues to understanding the evolution of T4 baseplate proteins [11].

The structures of gp10, gp11 and gp12 can be superimposed onto each other (Figure (Figure5)5) suggesting that the three proteins have evolved from a common primordial fold, consisting of an α-helix, a three-stranded β-sheet almost perpendicular to the helix, and an additional 2 or 3 stranded β-sheet further away from the helix (Figure (Figure6).6). This structural motif is decorated by big loops inserted in various regions of the core fold, thus obscuring visual comparison. It is of significance that the three proteins are translated from the same polycistronic mRNA and are sequential in the genome. Furthermore, all three proteins are on the periphery of the baseplate and interact with each other. Apparently, over the course of the T4 evolution, these proteins have become more functionally specialized and have acquired or discarded subdomains that define the functions of the present proteins.

Figure 6
Comparison of gp10 with other baseplate proteins; reprinted from [11]. A, Stereo view of the superposition of gp10, gp11, and gp12. For clarity, the finger domain of gp11 and the insertion loop between β-strands 2 and 3 of gp12 are not shown. ...

In addition to its structural role in the baseplate, gp8 functions as a chaperone for folding of gp6 (Table 2), which is insoluble unless co-expressed with gp8 [7]. Although wild type gp6 could not be crystallized, the structure of a gp6 mutant, constituting the C-terminal part of the protein (residues 334 - 660) has been determined [7]. The structure is a dimer, which fits well into the cryoEM map of both, the hexagonal and star-shaped baseplates [7].

Structure of the baseplate in the hexagonal conformation

The structure of the baseplate in the hexagonal conformation was studied both by using a phage mutant that produces the baseplate-tail tube complex (a g18¯/g23¯ double mutant), as well as by using wild type phage [5,47]. The star conformation was examined by treating the phage with 3 M urea in a neutral pH buffer [6] causing the tail to contract, but retaining the DNA in the head. This particle mimics the phage after it has attached to the host cell surface. Three-dimensional cryoEM maps of the baseplate and the entire tail in either conformation were calculated at resolutions of 12 Å and 17 Å, respectively (Figure (Figure7).7). The available crystal structures were fitted into these maps.

Figure 7
CryoEM reconstructions of the T4 tube-baseplate complex (A, B) and the tail in the extended (C) and contracted (D) conformation. Constituent proteins are shown in different colors and identified with the corresponding gene names. reprinted from [5,47 ...

The hexagonal baseplate is a dome-like structure with a diameter of about 520 Å around its base and about 270 Å in height. Overall, the structure resembles a pile of logs because its periphery is composed of fibrous proteins. The gp5-gp27 complex forms the central hub of the baseplate (Figure (Figure7B).7B). The complex serves as a coaxial continuation of the tail tube. Gp48 and/or gp54 are positioned between the gp27 trimer and the tail tube, comprised of gp19. The gp5 β-helix forms the central needle that runs along the dome's axis. A small protein with a MW of ~23 kDa is associated with the tip of the gp5 β-helix (Figure (Figure7B).7B). The identity of this protein is unclear, but the mass estimate suggests that it could be gp28. The tape measure protein, gp29, is almost completely disordered in the baseplate-tail tube structure. It is unclear whether gp29 degrades during the sample preparation or its structure does not agree with the sixfold symmetry assumed in generating the cryoEM map.

The earlier cross-linking and immuno-staining analysis of interactions between the baseplate wedge proteins turned out to be in good agreement with the later cryoEM results [48-50]. This is impressive considering the limitations of the techniques employed in the earlier studies. In agreement with the earlier findings, the new high resolution data show that gp10, gp11 and gp12 (the short tail fibers) constitute a major part of the baseplate's periphery. Gp9, the long tail fiber attachment protein, is also on the periphery, but in the upper part of the baseplate dome. Gp8 is positioned slightly inwards in the upper part of the baseplate dome and interacts with gp10, gp7 and gp6. The excellent agreement between the crystallographic and EM data resulted in the unambiguous locating of most of the proteins in the baseplate.

Six short tail fibers comprise the outermost rim of the baseplate. They form a head-to-tail garland, running clockwise if viewed from the tail towards the head (Figure (Figure8).8). The N-terminus of gp12 binds coaxially to the N-terminal domain of the gp10 trimer, and the C terminus of one gp12 molecules interacts with N terminus of the neighboring molecule. The fiber is kinked at about its center, changing its direction by about 90°, as it bends around gp11. The C-terminal receptor-binding domain of gp12 is 'tucked under' the baseplate and is protected from the environment. The garland arrangement controls the unraveling of the short tail fibers, which must occur on attachment to the host cell surface.

Figure 8
Details of the T4 baseplate structure; reprinted from [5]. Proteins are labeled with their respective gene numbers. A, The garland of short tail fibers gp12 (magenta) with gp11 structures (light blue Cα trace) at the kinks of the gp12 fibers. ...

Gp10 and gp7 consist of three separate domains each, connected by linkers (Figure (Figure8B).8B). Gp7 is a monomer, and it is likely that each of its domains (labeled A, B and C in Figure Figure8B)8B) is a compact structure formed by a single polypeptide chain. Gp10, however, is a trimer, in which the three chains are likely to run in parallel and each of the cryoEM densities assigned to gp10 domains is threefold symmetric. The angles between the threefold axes of these domains are close to 60°. This is confirmed by the fact that the trimeric gp10_397C crystal structure fits accurately into one of the three domains assigned to gp10. At the boundary of each domain, the three gp10 chains come close together thus creating a narrowing. Interestingly, the arrangement of gp10 domains is maintained in both conformations of the baseplate suggesting that these narrow junctions are not flexible. A total of 23% of the residues in the N-terminal 200 residues of gp10 are identical and 44% of the residues have conservative substitutions when compared to the N-terminal and middle domains of T4 gp9. A homology model of the N-terminal part of gp10 agrees reasonably well with the cryoEM density assigned to the gp10 N-terminal domain. The threefold axis of this domain in the cryoEM density coincides with that of the N-terminal part of gp12, which is attached to it. The middle domain of gp10 is clamped between the three finger domains of gp11.

Gp6, gp25 and gp53 form the upper part of the baseplate dome and surround the hub complex. The cryoEM map shows that the gp6 monomer is shaped like the letter S. Six gp6 dimers interdigitate and form a continuous ring constituting the backbone of the baseplate (Figures (Figures88 and and9).9). Gp6 is the only protein in the baseplate, which forms a connected ring in both conformations of the baseplate. The N- and C-terminal domains of each gp6 monomer interact with two different neighboring gp6 molecules, i.e. the N terminal domain of chain 'k' interacts with the N terminal domain of chain 'k+1', whereas the C-terminal domain of chain 'k' interacts with the C terminal domain of chain 'k-1'. It is thus possible to distinguish two types of gp6 dimers, depending on whether the N or C terminal domains of the two molecules are associated (Figure (Figure99).

Figure 9
Arrangement of gp6, gp25 and gp53 in the baseplate; reprinted from [7]. A, B, Gp6 is shown in magenta for the "hexagonal" dome-shaped baseplate (left) and in blue for the star-shaped baseplate (right). The C-terminal part of gp6 corresponds to the crystal ...

As there are only two molecules of gp6 per wedge, either the N-terminal or the C-terminal dimer has to assemble first (the intra-wedge dimer) and the other dimer is formed when the wedges associate into the ring structure (the inter-wedge dimer). Mutagenesis suggests that the Cys338 residue is critical for forming the N-terminal dimer, which therefore is likely to form the intra-wedge dimer [7]. The crystal structure represents the C-terminal inter-wedge dimer [7].

This finding is further supported by the baseplate assembly pathway. During assembly of the wedge, gp6 binds only after the attachment of gp8 [23,25]. Although a dimer of gp8 and a dimer of gp6 are present in each wedge [25], in the cryoEM baseplate map a single chain of the gp6 dimer interacts with a single chain of the gp8 dimer, whereas the other chain of the same gp6 dimer interacts with gp7. Together, gp8 and gp7 form a platform for binding of the N-terminal dimer of gp6, suggesting that the N-terminal dimer forms first during the assembly of the baseplate wedge, whereas C-terminal gp6 dimers form after six wedges associate around the hub.

The structures of the baseplate in the sheath-less tail tube assembly and in the complete tail are very similar, except for the position of gp9 (Figure (Figure7)7) [5,47]. The N-terminal domain of gp9 binds to one of the gp7 domains, but the rest of the structure is exposed to the solution. The long tail fibers attach coaxially to the C-terminal domain of gp9. This arrangement allows gp9 to swivel, as a rigid body, around an axis running through the N-terminal domain, allowing the long tail fiber to move. In the extended tail structure, the long tail fibers are retracted and aligned along the tail (Figure (Figure7c),7c), whereas the tail tube-baseplates lack the long tail fibers. Thus, in the extended tail, the gp9 trimers point along the fibers, whereas in the tube-baseplate complexes, gp9 molecules are partially disordered due to their variable position and point sideways, on average. This variation in the positioning of gp9 is required to accommodate the full range of positions (and hence motion) observed for the long tail fibers [51].

Structure of the baseplate in the star conformation and its comparison with the hexagonal conformation

The star-shaped baseplate has a diameter of 610 Å and is 120 Å thick along its central sixfold axis. The central hub is missing because it is pushed through and replaced by the tail tube (Figure (Figure10).10). Despite large changes in the overall baseplate structure, the crystal structures and the cryoEM densities of proteins from the hexagonal baseplate can be fitted into the star shaped baseplate. This indicates that the conformational changes occur as a result of rigid body movements of the constituent proteins and/or their domains.

Figure 10
Comparison of the baseplate in the two conformations; reprinted from [5]. A and B, Structure of the periphery of the baseplate in the hexagonal and star conformations, respectively. Colors identify different proteins as in the other figures: gp7 (red), ...

The largest differences between the two conformations are found at the periphery of the baseplate. In the hexagonal conformation, the C-terminal domain of gp11 points away from the phage head, and its trimer axis makes a 144° angle with respect to the six-fold axis of the baseplate (Figure (Figure10).10). In the star conformation, however, the gp11 C-terminal domain points towards the phage head, and the trimer axis makes a 48° angle with respect to the baseplate sixfold axis. Thus, upon completion of the baseplate's conformational change, each gp11 molecule will have rotated by almost 100° to associate with a long, instead of a short tail fiber. The long and short tail fibers compete for the same binding site on gp11. The interaction between gp10 and gp11 is unchanged in the two conformations. As a result, the entire gp10-gp11 unit rotates by ~100° causing the N-terminal domain of gp10 to change its orientation and point towards the host cell surface (Figure (Figure10).10). The short tail fiber, which is coaxially attached to the N-terminal domain of gp10, rotates and unfolds from under that baseplate and extends the C-terminal receptor-binding domain towards the potential host cell surface. In addition to the gp10-gp11 complex rotation and short tail fiber unraveling, domain A of gp7 swivels outwards by about 45° and alters its association with gp10, making the baseplate structure flat. This rearrangement brings the C-terminal domain of gp10 into the proximity of gp9 and allows the latter to interact with gp8. The structural information supports the hypothesis that the hexagonal-to-star conformational change of the baseplate is the result of a reorientation of the pins (gp7, gp10, gp11) [50] and additionally shows that the transformation also involves rearrangements of gp8, gp9, and gp12 situated around the periphery of the baseplate.

The association of gp10, gp11 and gp12 into a unit that can rotate by 100° is tight, but appears to be non-covalent. However, there could be at least one covalent bond that attaches this unit to the rest of the baseplate. Cys555, the only conserved cysteine in gp10 among all T4-like phages, is one of the residues that are involved in interactions between gp10 and domain B of gp7 in the baseplate. This cysteine might make a disulfide bond with one of eight cysteine residues in gp7, causing the gp10-gp11-gp12 complex and domain B of gp7 to act as a single rigid body during the conformational change of the baseplate. Unfortunately, residues 553-565 are disordered in the crystal structure of gp10_397C, and the exact structure of the region interacting with gp7 is uncertain. This is not surprising, as these residues might be prone to adopting various conformations, because the interaction with gp7 is not threefold symmetric.

The central part of the baseplate, which is comprised of gp6, gp25 and gp53, displays a small, but noticeable change between the two conformations of the baseplate. Both the N-terminal and C-terminal dimer contacts in the gp6 ring are maintained, but the angle between the gp6 domains changes by about 15°, accounting for the slight increase in the gp6 ring diameter (Figures (Figures99 and and10).10). Therefore, the gp6 ring appears to have two functions. It is the inter-wedge 'glue', which ties the baseplate together and it is also required for maintaining the baseplate integrity during the change from hexagonal to star shaped conformations. At the same time, the gp6 ring is a framework to which the motions of other tail proteins are tied. The N-terminal domain of gp6 forms a platform onto which the first disk of the tail sheath subunits is added when the sheath it assembled. Therefore, the change in the gp6 domain orientations could be the signal that triggers the contraction of the sheath.

Structure of the tail sheath in the extended and contracted conformation

Crystal structure of gp18

Recombinant, full-length gp18 (659 residues) assembles into tubular polymers of variable lengths called polysheaths, which makes crystallization and high resolution cryoEM studies difficult. However several deletion mutants that lack polymerization properties have been crystallized [52]. The crystal structures of two of these mutants have been determined. One of these is of a protease resistant fragment (gp18PR) consisting of residues 83-365. The other, called gp18M, is of residues 1-510 in which the C-terminal residue has been replaced by a proline (Figure (Figure11).11). The crystal structure of the gp18PR fragment has been refined to 1.8 Å resolution and the structure of the larger gp18M fragment was determined to 3.5 Å resolution [53].

Figure 11
Structures of the gp18 deletion mutants reprinted from [53]. A, A ribbon diagram of the gp18PR mutant. The N terminus is shown in blue, the C terminus in red and the intermediate residues change color in spectral order. B, C, A ribbon diagram of the gp18M ...

The structure of gp18M includes that of gp18PR and consists of Domains I, II and III (Figure (Figure11).11). Domain I (residues 98-188) is a six-stranded β-barrel plus an α-helix. Domain II (residues 88-97 and 189-345) is a two layer β-sandwich, flanked by four small α-helices. Together, domains I and II form the protease resistant fragment gp18PR. Domain III (residues 24-87 and 346-510) consists of a β-sheet with five parallel and one anti-parallel β-strands plus six α-helices surrounding the β-sheet. The 24 N-terminal residues as well as residues 481 to 496 were not ordered in the gp18M crystal structure. The N and C termini of the structure are close in space, suggesting that the first 24 residues and residues 510-659 form an additional domain, Domain IV, which completes the structure of the full-length protein. The overall topology of the gp18 polypeptide chain is quite remarkable. Domain I of gp18 is an insertion into Domain II, which, in turn, is inserted into Domain III, which is inserted between the N and C termini comprising domain IV.

Fitting of the gp18M structure into the cryoEM map of the tail showed that the protease resistant part of gp18 is exposed to the solution, whereas the N and C termini, which form Domain IV, are positioned on the interior of the tail sheath (Figure (Figure12).12). The exposed and buried residues in each conformation of the sheath are in agreement with previous immuno-labeling and chemical modification studies [54,55]. Domain I of gp18 is protruding outwards from the tail and is not involved in inter-subunit contacts. The other three domains form the core of the tail sheath with Domains III and IV being the most conserved parts of tail sheath proteins among T4-related bacteriophages (Figure (Figure12).12). Despite the fact that Domain I has apparently no role in gp18-gp18 interactions, this domain binds to the baseplate in the extended tail sheath. Thus, one of the roles of Domain I may be to initiate sheath assembly and contraction. Domain I also binds the long tail fibers when they are retracted. It was previously shown that three mutations in Domain I (G106→S, S175→F, A178→V) inhibit fiber retraction [56]. These mutations map to two loops close to the retracted tail fiber attachment site on the surface of the extended tail sheath, presumably abrogating binding of the tail fibers.

Figure 12
Arrangement of the gp18 domains in the extended (A) and the contracted (B) tail reprinted from [53]. Domains I, II and III of gp18M are colored blue, olive green and orange red, respectively. The same color scheme is used in (C) the linear sequence diagram ...

Structure of the extended sheath and the tube

The 240 Å-diameter and 925 Å-long sheath is assembled onto the baseplate and terminates with an elaborate 'neck' structure at the other end (Figures (Figures1313 and and14).14). The 138 copies of the sheath protein, gp18, form 23 rings of six subunits each stacked onto one another. Each ring is 40.6 Å thick and is rotated by 17.2° in a right-handed manner relative to the previous ring. The sheath surrounds the tail tube, which has external and internal diameters of 90 Å and 40 Å, respectively. The area of contact between the adjacent gp18 subunits with the neighboring gp18 subunit in the ring above is significantly greater than that between neighboring subunits within a ring (about 2,000 Å2 versus 400 Å2). Thus, the sheath is a six-fold-symmetric, six-start helix (Figure (Figure1313).

Figure 13
Connectivity of the sheath subunits in the extended (A) and contracted (B) tail sheath reprinted from [53]. The cryoEM map of the entire tail is shown on the far left. Immediately next to it, the three adjacent helices (in pink, blue and green) are shown ...
Figure 14
The structure of the collar and whiskers; reprinted from [5]. A, Cutaway view of the tail neck region. B, The structure of the gp15 hexameric ring in the extended and contracted tail. C, and D, Side and top views of the collar structure. For clarity, ...

The tail tube (also called the "core" in the literature) is a smooth cylinder, lacking easily discernable surface features. Nevertheless, it can be segmented into individual subunits of the tail tube protein gp19 at an elevated contour level. The subunits are arranged into a helix having the same helical parameters as those found for the gp18 helix.

Structure of the contracted sheath

The contracted sheath has a diameter of 330 Å and is 420 Å long (Figures (Figures77 and and13).13). The gp18 subunits form a six-start right-handed helix with a pitch of 16.4 Å and a twist angle of 32.9° situated between radii of 60 Å and 165 Å. The sheath has an inner diameter of 120 Å and does not interact with the 90 Å-diameter tail tube, in agreement with previous observations [57]. Upon superimposing the midsection of the sheath onto itself using the helical transformation, the correlation coefficient was found to be 0.98, showing that there is little variation in the structure of the gp18 subunits and that the sheath contracts uniformly.

The structure of gp18 subunit in the contracted tail is very similar to that in the extended tail. The internal part of the gp18 subunits retains its initial six-start helical connectivity, which is formed when the sheath is first assembled onto the tail tube. This helix has a smaller diameter in the extended conformation and interacts with the tail tube, thus stabilizing the sheath. This was further confirmed by fitting of the gp18M crystal structure into the cryoEM density maps of the tail sheath. The structure fits as a rigid body into both the extended and contracted conformations of the sheath, suggesting that contraction occurs by sliding of individual gp18 subunits over each other with minimal changes to the overall fold of the sheath protein (Figure (Figure12).12). During contraction each subunit of gp18 moves outwards from the tail axis while slightly changing its orientation. The interactions between the C-terminal domains of gp18 subunits in the extended confirmation appear to be preserved in the contracted form, maintaining the integrity of the sheath structure. However, the outer domains of gp18 change interaction partners and form new contacts. As a result, the interaction area between the subunits increases about four times.

The helical symmetry of the sheath shows that the first and last layers in the extended and contracted conformations are related by a 378.4° (1.05 turns) rotation and 723.8° (2.01 turns) rotation, respectively. Assuming that the association of the sheath and tail tube subunits in the neck region is fixed, the tube will thus rotate by 345.4° - almost a full turn - upon tail contraction (Figure 13C).

Although the diameter of the tube is the same, the symmetry and gp19 subunit organization bear no resemblance to that of the extended or contracted sheath. The tail tube subunits in phage with a contracted tail appear to have an organization that is slightly different to that found in the virus with an extended sheath.. However this might be an artifact of the image reconstruction procedure used to view the details of the tail tube, because the tail tube is internal to the sheath, which has a repetitive structure that might have influenced the reconstruction procedure.

The neck region lacks the fibritin and other proteins in the contracted tail map. This sample was prepared by diluting a concentrated phage specimen into 3 M urea. There is little doubt now, that this harsh treatment caused the observed artifacts. Recent experiments showed that the fibritin and other proteins remain associated with the phage particle if the latter is subjected to slow dialysis into 3 M urea. In this procedure, the tails uniformly contract and their structure is identical to that found in the earlier studies (A. Aksyuk, unpublished observations).

Structure of the neck region

The neck consists of a several sets of stacked hexameric rings consisting of gp3, gp15, and gp13 or gp14 (Figure (Figure14).14). The gp3 terminates the tail tube, followed by gp15, and then by gp13 and/or gp14 closest to the head. In the cryoEM reconstruction of the wild type phage, the channel running through the length of the gp19 tube is filled with a roughly continuous density at an average diameter of ~20 Å. This might be the extended molecule(s) of gp29 tape measure protein or phage DNA. The former proposition is more likely, as the tail channel is blocked by the gp15 hexamer, which forms a closed iris with an opening of only 5-10 Å and should prevent the DNA from entering the tail.

The neck is surrounded by a 300 Å diameter and 40 Å thick collar, consisting at least in part of fibritin (gp wac) [58]. Fibritin is a 530 Å-long and 20 Å-diameter trimeric fiber [59]. The atomic structure of the N- and C-terminal fragments of fibritin is known [60,61]. The rest of this fiber has a segmented coiled coil structure and can be modeled using the known structure and the repetitive nature of its amino acid sequence [59-61]. The cryoEM map of wild type T4 could be interpreted with the help of this model.

Each of the six fibritin trimers forms a tight 360° loop, which together create the main part of the collar and the whiskers (Figure (Figure14).14). Both the N and C termini of the fibritin protein attach to the long tail fiber. The C-terminal end binds to the 'kneecap' region of the long tail fiber, comprised of gp35, whereas the N terminus most probably binds to the junction region of gp36 and gp37. The fibritin's 360° loop interacts with gp15 and is in the N-terminal part of the protein. This is in agreement with earlier studies that found that the N terminus of fibritin is required for its attachment to the phage particle. The six fibritins and the long tail fibers are bridged together by six copies of an unknown fibrous protein to form a closed ring. This protein is about 160 Å long and 35 Å in diameter.

Tail Fiber Structure and Assembly

Overall organization and subunit composition

The long tail fibers of bacteriophage T4 are kinked structures of about 1440 Å long with a variable width of up to about 50 Å. They can be divided into proximal and distal half-fibers, attached at an angle of about 20° [62]. In adverse conditions for phage multiplication, the long tail fibers are in a retracted conformation, lying against the tail sheath and head of the bacteriophage. In the extended conformation, only the proximal end of the fiber is attached to the baseplate. The long tail fibers are responsible for initial interaction with receptor molecules [2]. The distal tip of the long tail fibers can recognize the outer membrane protein C (ompC) or the glucosyl-α-1,3-glucose terminus of rough LPS on E. coli [63]. Titration experiments showed that the phage particle has to carry at least three long tail fibers to be infectious [64].

The long tail fiber is composed of four different gene products: gp34, gp35, gp36 and gp37 (Figure (Figure15)15) [65]. The proximal half-fiber, or the "thigh", is formed by a parallel homo-trimer of gp34 (1289 amino acids or 140 kDa per monomer). In the intact phage, the N-terminal end of gp34 is attached to the baseplate protein gp9 [8], while the C-terminal end interacts with the distal half-fiber, presumably with gp35 and/or gp36. Gp35 (372 residues; 40 kDa and present as a monomer) forms the "knee" and may be responsible for the angle between the proximal and distal half-fibers. The distal half-fiber is composed of gp35, trimeric gp36 (221 amino acids, 23 kDa) and gp37 (1026 amino acids; 109 kDa). The gp36 protein subunit is located at the proximal end of the distal half-fiber, forming the upper part of the "shin", while gp37 makes up the rest of the shin, including the very distal receptor-recognizing tip (or "foot"), which corresponds to the C-terminal region of gp37.

Figure 15
Gene structure, assembly pathway and domain organization of the bacteriophage T4 long tail fibers. Chaperone interactions are shown as grey arrows. Domains of the proximal tail fiber are named P1-5 and of the distal half D1-11; gp35, or the knee-cap (KC) ...

The four structural genes of the long tail fiber and the chaperone gp38 are located together in the T4 genome. Genes 34 and 35 are co-transcribed from a middle-mode promoter, gene 36 from a late promoter, while genes 37 and 38 are co-transcribed from another promoter [66]. The gp34 protein is the largest T4 protein, followed by the baseplate protein gp7 the second-largest protein and gp37 the third-largest protein in the baseplate.

Despite their extended dimensions, the long tail fibers appear to be stiff structures, because no kinked half-fibers have been observed in electron micrographs. Moreover, the angle between the half fibers in the complete fiber does not deviate very far from 20° on average. The stiffness may be necessary for transmitting the receptor recognition signal from the tip of the fiber to the baseplate and for bringing the phage particle closer to the cell surface as the baseplate changes its conformation. No atomic resolution structures for the long tail fibers, their components or their chaperones (see next section) have yet been published.

In the cryoEM reconstruction of the wild-type T4, the fibers are in the retracted configuration (Figure (Figure7),7), likely caused by the unfavorable for infection conditions of the cryoEM imaging procedure (a very high phage concentration and a very low salt buffer). The density corresponding to the long tail fibers is quite poor (Figure (Figure7).7). This is likely caused by the variability of the positions of the long tail fibers. The 700 Å-long proximal half-fiber and the about 2/3 of the 740 Å-long distal part are present in the cryoEM map. The proximal half-fiber is bent around the sheath, forming about a quarter of a right-handed helix.

Assembly: folding chaperones and attachment proteins

A phage-encoded molecular chaperone, gp57A, is required for the correct trimerization of long tail fiber proteins gp34 and gp37 [62]; and for the short tail fiber protein gp12 [67] (Table 2). Gp57A appears to be a rather general T4 tail fiber chaperone and is needed for the correct assembly of the trimeric short and long tail fiber proteins gp12, gp34, and gp37 [68]. Gp57A is a small protein of 79 residues (8,613 Da) that lacks aromatic amino acids, cysteines and prolines. In vitro, it adopts different oligomeric states [44]. The specific chaperone gp38 must be present [68] for the correct trimeric assembly of gp37. The molecular basis of the gp38 and gp57A chaperone activities are unclear, but it has been proposed that gp57A functions to keep fiber protein monomers from aggregating unspecifically, while gp38 may bring together the C-terminal ends of the monomers to start the folding process [62]. Qu et al. [69] noted that extension of a putative coiled-coil motif near the C-terminal end of gp37 bypasses the need for the gp38 chaperone. The extended coiled-coil may function as an intramolecular clamp, obviating the need for the intermolecular gp38 chaperone.

Two parts of the long tail fiber (the distal and proximal half-fibers) assemble independently. The three proteins of the distal half-fiber interact in the following order. Initially trimeric gp36 binds to the N-terminal region of gp37, and then monomeric gp35 binds to gp36, completing the assembly of the distal half-fiber. Joining of the two half-fibers presumably takes place spontaneously.

Attachment of the assembled long tail fiber to the phage particle is promoted by gp63 and the fibritin (gp wac) [62], although neither of these proteins is absolutely essential (Table (Table2).2). Unlike gp63, the fibritin is a component of the complete phage particle and constitutes a major part of the neck complex (see above). In the absence of the fibritin, the long tail fibers attach to fiberless particles very slowly. The whiskers are also be involved in the retraction of the long tail fibers under unfavorable conditions. Gp63 has RNA ligase activity and may function as such in infected cells. However, the isolation of gene 63 mutants that affect RNA ligase activity, but not tail fiber attachment activity suggests that gp63 is a bifunctional protein that promotes two physiologically unrelated reaction [70].

Structural studies of the long tail fiber

Scanning transmission electron microscopy of stained and unstained particles has been used to study the structure of intact long tail fibers, proximal half-fibers and distal half-fibers [65]. The proximal half-fiber, gp34, consists of an N-terminal globular domain that interacts with the baseplate. It is followed by a rod-like shaft about 400 Å long that is connected to the globular domain by a hinge. The rod domain seen by EM correlates with a cluster of seven quasi-repeats (residues 438 to 797 [65]), which are also present six times in gp12 and once in gp37. One of these repeats is resolved in the crystal structure of gp12 (amino acids 246 to 290 [12]). This structural motif consists of an α-helix and a β-sheet. The proximal half-fiber terminates in three globular domains arranged like beads on a stick.

EM has shown that the proximal and distal half-fibers are connected at an angle of about 160°. A hinge is present between the proximal and distal half-fibers, forming the "knee". Density, associated with the presence of gp35, a monomer in the long tail fiber, bulges asymmetrically out on the side of the fiber forming the reflex angle (i.e. at the opposite side of the obtuse angle) [65].

The distal half-fiber, composed of gp36 and gp37, consists of ten globular domains of variable size and spacing, preceding a thin end domain or "needle" with dimensions of about 150 by 25 Å [65]. Based on its relative molecular mass (compared to that of the other long tail fiber components), gp36 should make up about one sixth of the distal half-fiber and thus likely composes at least the two relatively small proximal globules, the thin rod in between them, and perhaps the third globule. The remaining seven or eight globules and the needle or "foot" would then be gp37. A single repeat, similar to those also present in gp12 and gp34 is found in the N-terminal region of gp37, (amino acids 88-104). Residues 486 to 513 of gp37 show strong similarity to residues 971 to 998 of gp34 and are likely to form a homologous structural motif. Another sequence similarity has been observed between residues 814-860 and residues 342-397 of gp12 [65]. In gp12, these residues form the collar domain [12,14]. Gp34, gp36, and gp37 are predicted to mainly contain β-structure and little α-helical structure. However, their limited sequence similarity with each other, with the T4 short tail fiber protein gp12 and with other fiber proteins makes structure prediction difficult. Streptococcus pyogenes prophage tail fiber was shown to contain an extended triple β-helix in between α-helical triple coiled-coil regions [71], while the bacteriophage P22 tail needle gp26 has a very small triple β-helical domain and extensive stable α-helical triple coiled-coil regions [72]. A general principle may be that folding of the above mentioned fiber proteins starts near the C-terminus, as is the case for the adenovirus vertex fibers [73].

In general, trimeric fibrous proteins require a chaperone 'module' for folding. This module can be a small domain of the same polypeptide chain or a separate protein (or several proteins) [74]. Simultaneous co-expression of gp37, gp57A and gp38 has been used to obtain mg-amounts of soluble gp37 [75]. Correct folding of the trimeric protein was assessed by gel electrophoresis, cross-linking and transmission electron microscopy studies. The C-terminal fragments of gp37 appear to be folded correctly, showing that folding behavior of gp37 resembles that of gp12 [38].

The Infection Mechanism

Structural transformation of the tail during infection

The following observations suggest that the hexagonal conformation of the baseplate and the extended state of the sheath both represent high energy metastable assemblies. Purified baseplates have been shown to switch spontaneously into the star conformation [50]. In the absence of either the baseplate or the tail tube, the sheath assembles into a long tubular structure similar to that of the contracted sheath [57]. The tail sheath contraction is irreversible, and the contracted tail structure is resistant to 8 M urea [76]. These observations suggest that the baseplate in the hexagonal conformation together with its extended sheath can be compared to an extended spring ready to be triggered [77].

By combining all the available experimental information on T4 infection, it is possible to describe the process of attachment of the phage to the host cell in some detail (Figure (Figure16,16, Movie 2 http://www.seyet.com/t4_virology.html). The long tail fibers of the infectious phage in solution are extended, and most possibly move up and down due to the thermal motion [51,78,79]. Attachment of one of the fibers to the cell surface increases the probability for the other fibers to find cell surface receptors. The attachment of three or more of the long tail fibers to their host cell receptors is possible only when they point towards the host cell surface. This configuration of the tail fibers orients the phage particle perpendicular to the cell surface.

Figure 16
Baseplate conformational switch schematic reprinted from [6]. A and B, The phage is free in solution. The long tail fibers are extended and oscillate around their midpoint position. The movements of the fibers are indicated with black arrows. The proteins ...

As the gp9 trimer is coaxial with the proximal part of the long tail fiber, gp9 proteins swivel up and down following the movements of the long tail fibers as the phage particle travels in search of a potential host cell. When the long tail fibers attach to the host cell surface and their proximal parts point down, several new protein-protein interactions at the periphery of the baseplate are initiated: 1) gp9 binds to the C-terminal domain of gp10; 2) the long tail fiber binds to a gp11 trimer. These interactions are likely causing gp11 to dissociate from gp12 leading to destabilization of the gp12 garland. The baseplate then unlocks from its high energy metastable hexagonal state. The A domain of gp7 swivels outwards and the entire gp10-gp11-gp12 module rotates, causing the C-terminal domains of the short tail fibers to point towards the host cell surface, thus preparing them for binding to the host cell receptors. Gp9 and the long tail fibers remain bound to the baseplate pins (the gp7-gp10-gp11 module), during this transformation.

During the conformational change of the baseplate, the long tail fibers are being used as levers to move the baseplate towards the cell surface by as much as 1000 Å. As the lengths of the two halves of the fiber are close to 700 Å each, such a large translation is accomplished by changing the angle between them by about 100°.

The conformational changes, which are initiated at the periphery of the baseplate, would then spread inwards into the center of the baseplate causing the central part of the baseplate (gp6, gp25 and gp53) to alter its conformation and thus initiating sheath contraction. The process of sheath contraction is accomplished by rotating and sliding the gp18 sheath subunits and progresses through the entire sheath starting at the baseplate (Movie 3 http://www.seyet.com/t4_virology.html). The contracting sheath then drives the tail tube into the host membrane. The baseplate hub, which is positioned at the tip of the tube, will be the first to come in contact with the membrane. The membrane is then punctured with the help of the gp5 C-terminal β-helix and the yet unidentified protein (gp28?), which caps the tip of the gp5 β-helix. Subsequent tail contraction drives the tail tube further, and the entire gp5-gp27 complex is then translocated into the periplasmic space. The three lysozyme domains of the gp5 trimer start their digestion of peptidoglycan after the gp5 β-helix has dissociated due to the steric clashes with the peptidoglycan. This process results is a hole in the outer part of the cell envelope, allowing the tail tube to interact with the cytoplasmic membrane initiating phage DNA transfer. As mentioned above, the tail contraction involves rotation of the tail tube by an almost complete turn. Thus, the tail tube drills, rather than punctures, the outer membrane.

The fate and function of gp27 in the infection is unknown. Gp27 does not appear to form a trimer in the absence of gp5 [13], but it is possible that gp27 might be able to maintain its trimeric form upon its association with the tail tube because the gp27 trimer is a smooth coaxial continuation of the tail tube with a 25 Å diameter channel. Furthermore, the lysozyme-containing N-terminal part of gp5 (gp5*) might be able to dissociate from gp27 in the periplasm (due to the lower pH [13]) to open the gp27 channel. Gp27 may thus form the last terminal pore of the tube through which the phage DNA and proteins enter the host cell. Possibly, gp27 might interact with a receptor in or at the cytoplasmic membrane.

The above speculation that the gp27 trimer may serve as the terminal opening of the tail tube is supported by the crystal structure of a gp27 homolog called gp44 from bacteriophage Mu (a contractile tail phage) [80]. Although T4 gp27 and Mu gp44 have no detectible sequence similarity, the two structures have very similar folds [80]. Gp44, however, forms a stable trimer in solution and most probably serves as a centerpiece of the Mu baseplate. Gp45 is a glycine-rich protein from the Mu tail, making it a possible ortholog of gp5.

Conclusion

Contractile tail evolution and relation to other biological systems

There is building a body of evidence proving that all tailed phages have a common ancestor. The evolutionary relationship cannot be detected in their amino acid sequences, but structural studies show that capsid proteins of all tailed phages have a common fold (the HK97 fold) and that the portal proteins are homologous [81-83]. As the DNA packaging processes in all tailed phages are similar, their ATPases and many other structural proteins are also most probably homologous.

The recently discovered and incompletely characterized bacterial type VI secretion system (T6SS) appears to be related to a phage tail [84]. The T6SS is one of the most common secretion systems present in at least 25% of all Gram-negative bacteria, and is associated with an increased virulence of many pathogens [85]. Similar to other secretion systems, T6SS genes are clustered in pathogenicity islands containing 20 or more open reading frames. The hallmark of the T6SS expression is the presence of the conserved Hcp protein in the external medium [86]. VgrG proteins represent the other most common type of protein found secreted in a T6SS-dependent fashion. It was shown that in Vibrio cholerae, VgrG-1 is responsible for T6SS-dependent cytotoxic effects of V. cholerae on host cells including Dictyostelium discoideum amoebae and J774 macrophages [87]. The C terminus of VgrG-1 encodes a 548 residue-long actin cross-linking domain or ACD [87], which is also found embedded in a secreted toxin of V. cholerae called RtxA. VgrG orthologs in bacterial species other than V. cholerae carry a wide range of putative effector domains fused to their C termini [87].

The crystal structure of the N-terminal fragment the Escherichia coli CFT073 VgrG protein encoded by ORF c3393 shows a significant structural similarity to the gp5-gp27 complex, despite only 13% sequence identity [84]. The crystal structure of Hcp1 [88], the most abundant secreted protein in T6SS-expressing Pseudomonas aeruginosa strain PAO1, shows that it is homologous to the tandem 'tube' domain of gp27, which interacts with the T4 tail tube. Hcp1 is a donut-shaped hexamer with external and internal diameters of 85 Å and 40 Å, respectively. These hexamers stack on top of each other head-to-tail to form continuous tubes in the crystals. Some Hcp proteins can form tubes in vitro [84]. The homology of these two key proteins to the phage tail proteins and the fact that VgrG is translocated across a lipid membrane into a target cell suggest that the T6SS machine and phage tails might have a common ancestor.

Many evolutionary questions deal with the chicken and egg paradox. Whether the phage tail has evolved from the T6SS or vice versa is one of those questions. Clearly, the phage and its host benefit from coexistence and are capable of exchanging not only small proteins and protein domains, but also large and sophisticated supramolecular assemblies.

Abbreviations

Gp: gene product; HEWL: hen egg white lysozyme; LPS: lipopolysaccharide; NAG: N-acetylglucosamine; NAM: N-acetlymuramic acid; ORF: open reading frame; RMSD: root mean square deviation; T4L: lysozyme of T4 phage encoded by gene e; T6SS: bacterial type VI secretion system.

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

PGL, FA and MJvR made a summary of the available data and wrote the text. MGR carried out a major revision of the text. PGL, VAK, AAA and SK prepared the figures. PGL and Seyet LLC created Movies 1 and 2. AAA created Movie 3. All authors read and approved the final manuscript.

Acknowledgements

This article is part of the series of reviews on "Bacteriophage T4 and its Relatives". We are very grateful to Jim Karam for organizing this series, which is an essential update to the previously published but now somewhat outdated books and reviews on T4.

Movies 1 and 2 were made in close collaboration with the Seyet LLC, a scientific visualization company. We thank Seyet for their support.

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