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Eur J Hum Genet. Nov 2010; 18(11): 1202–1208.
Published online Jul 14, 2010. doi:  10.1038/ejhg.2010.107
PMCID: PMC2987466

The PSEN1 I143T mutation in a Swedish family with Alzheimer's disease: clinical report and quantification of Aβ in different brain regions

Abstract

Early-onset dominantly inherited forms of Alzheimer's disease (AD) are rare, but studies of such cases have revealed important information about the disease mechanisms. Importantly, mutations in amyloid precursor protein (APP), presenilin 1 (PSEN1) and PSEN2, alter the APP processing and lead to an increased amyloid β-peptide (Aβ) 42/40 ratio. This, together with other studies on pathogenic mechanisms, show that Aβ42 is a major player in the etiology of AD. Here, we present a clinical and neuropathological description of a Swedish family with an I143T mutation in the PSEN1 gene, which gives rise to a severe form of AD. We also performed an extensive investigation on the concentration and distribution of Aβ species of different lengths in six brain regions from two mutation carriers. Our study showed that Aβ42 and a longer peptide, Aβ43, were present both in plaque cores and in total amyloid preparations, and were each clearly more frequent than Aβ40 in all examined regions, as shown by both mass spectrometry and immunohistochemistry.

Keywords: Alzheimer's disease, PSEN1 mutations, amyloid β-peptide variants, mass spectrometry, neuropathology

Introduction

Alzheimer's disease (AD) is a neurodegenerative disorder and a major cause of dementia, characterized by amyloid β-peptide (Aβ) deposits and neurofibrillary tangles in brain.1, 2, 3 Aβ is typically 40 or 42 amino acids long and is produced by the sequential cleavages of the amyloid precursor protein (APP) by β-secretase and γ-secretase.4 The presenilins (1 and 2) are important components of the γ-secretase complex, and harbor its active site and thereby influence its proteolytic effect. Aβ40 is produced at higher levels, but as Aβ42 is more hydrophobic and has a stronger tendency to polymerize into neurotoxic species, it seems to be of particular importance in AD pathogenesis.5, 6, 7 This is supported by studies on mutations in APP, presenilin 1 (PSEN1) and PSEN2, which show an increased Aβ42/40 ratio.8 Five mutations have previously been described in families from Sweden: two mutations in APP (the APP Swedish9 and the APP Arctic mutations10) and three in PSEN1 (M146V, H163Y,11 and R269H12). In the present study, we describe the clinical characteristics of a three-generational Swedish family found to have the I143T PSEN1 mutation, which causes a rapidly progressing type of early-onset AD (EOAD). The γ-secretase complex with the I143T mutation in PSEN1 has been suggested to produce Aβ peptides of different lengths.13 We recently reported that a longer variant, Aβ43, is present in plaque cores and in total amyloid preparations obtained from sporadic and familial AD brains, and was indeed found more frequently than Aβ40.14 A quantitative approach was used to study Aβ40, Aβ42, and Aβ43 in two mutation carriers diagnosed with a severe and aggressive form of EOAD by examining samples taken from six brain regions, including the frontal, parietal, temporal, and occipital cortex, as well as anterior hippocampus and cerebellum. High-performance liquid chromatography (HPLC) coupled with an electrospray ionization ion-trap mass spectrometer (MS) was used. In parallel, we performed immunohistochemistry on consecutive sections using antibodies directed against Aβ40, Aβ42, and Aβ43. In addition, secreted and cellular Aβ was analyzed in cultured fibroblasts from mutation carriers and controls using a highly sensitive ELISA.

Materials and methods

The study was approved by the Regional Ethics Committee in Stockholm and informed consent was obtained from the participants, or from a proxy.

Sample preparation for neuropathological examination and immunohistochemistry

The left halves of the brains were frozen after death and the right halves were fixed in 4% formaldehyde. Samples from the different regions, such as hippocampus, amygdala, basal ganglia, thalamus, mesencephalon, brainstem, and cerebellum, were collected from the fixed brains. In addition, one gross section of the cerebral hemispheres was collected from each patient.

Staining was performed on 5-μm-thick formaldehyde-fixed paraffin-embedded sections. Sections were routinely stained with hematoxylin and eosin, Luxol fast blue, Bielschowsky, and AT8 antibody. Some sections were also Congo stained to detect amyloid. Immunohistochemistry was performed on sections pre-treated with formic acid (FA; 88%) for 10 min. Staining was performed using a C-terminal-specific antibody diluted in Bond primary antibody diluents (Vision BioSystems Limited, Newcastle Upon Tyne, UK), 1:4000 for Aβ40 (BioSource, Camarillo, CA, USA), 1:50 for Aβ42 (BioSource), and 1:8000 for Aβ43 (GeneTex, Irvine, CA, USA). Preparations were visualized in a Bond Polymer Refine Detection system (Vision BioSystems Limited). The specificity of the Aβ43 antibody was tested as previously described.14

Molecular genetics

DNA was extracted from peripheral blood (cases III:1 and III:2) or from formalin-fixed paraffin-embedded tissue (case II:2). The PSEN1 gene exons 2–11, the coding part of exon 12, and at least 40 flanking nucleotides were sequenced in both directions using an ABI 3100 genetic analyzer and ABI PRISM BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA), with primer pairs available on request. The restriction enzyme Tsp45 (New England BioLabs, Ipswich, MA, USA) was used to confirm the presence of the mutation. The APOE genotype for case III:2 was obtained by the SNaPshot multiplex system (Applied Biosystems), whereas the genotypes for III:1 and II:2 were obtained from medical records.

Plaque core preparations

Plaque cores obtained from frozen frontal, parietal, temporal, and occipital cortex samples, as well as from the anterior hippocampus and cerebellum were prepared using the same procedure as described in Welander et al.14 Initially, 0.5–1 g of the brain tissue was homogenized in Tris-buffered saline (TBS; pH 7.4) supplemented with Complete protease inhibitor mixture (Roche Applied Science, Basel, Switzerland; 4 ml buffer per g of the tissue) using a mechanical pestle homogenizer (IKALaborteknik RW20, IKA Labortechnik, Staufen, Germany). The homogenate was centrifuged at 4000 g for 2 min at 4°C and the resulting pellet was then washed in TBS and stained overnight with Congo red (50% saturated in TBS). The pellet was filtered through a 100 μm nylon mesh (Millipore Corporation, Bedford, MA, USA), adjusted to 46% sucrose and centrifuged at 16 000 g for 1 h at 4°C, then followed by one more centrifugation of the pellet at 16 000 g for 5 min at 4°C and dilution in 1% SDS in TBS. After filtration through a 60-μm nylon mesh (Millipore Corporation) and centrifugation at 16 000 g for 5 min at room temperature (24°C), the pellet was washed three times in TBS buffer and centrifuged at 16 000 g for 5 min at room temperature (24°C). The final pellet was stored at −20°C.

Preparation of SDS-insoluble brain homogenates

A previously described protocol for SDS preparations was followed.14 In brief, the samples were homogenized as described above. The homogenate was centrifuged at 50 000 g for 1 h. The resulting pellet was re-homogenized in TBS with 1% SDS and centrifuged at 50 000 g for 1 h. This procedure was repeated three times. The final pellet was washed five times in TBS and stored at −20°C.

Dissolution and cleavage of brain samples

The purified plaque cores as well as pellets from the SDS preparations were dissolved in 80% FA and 4 mg of cyanogen bromide (CNBr) in a shaker at 600 r.p.m. overnight at 37°C. CNBr cleaves the methionine at the C-terminal end (generating Aβ36-x fragments). Samples were prepared for LC-MS/MS analysis by concentrating and desalting the samples using C 18 ZipTips (Millipore Corporation) according to the manufacturer's instructions, and then dried in a vacuum centrifuge (Maxi dry lyo, Heto, Alleröd, Denmark) and dissolved in 40 μl of 0.1% FA. Finally, 0.5 μl of the plaque core samples and the SDS preparations was injected into the LC-MS/MS analysis system.

Quantification

The synthetic Aβ peptides Aβ36–40, Aβ36–42, and Aβ36–43 (Bachem, Basel, Switzerland) were used as standards for quantifications as described previously.14 The peptides were dissolved in 80% FA and diluted in 0.1% FA. Standard curves were plotted by mixing Aβ36–40, Aβ36–42, and Aβ36–43 at a ratio of 20:20:1 and by injecting 1 μl of 500, 200, 50, and 20 fmol of Aβ36–40 and Aβ36–42, and 25, 10, 2.5, and 1 fmol of Aβ36–43 in the same solution. The monoisotopic masses for the Aβ fragments were plotted as extracted ion chromatograms. The peak areas were used in the equations from the standard curves to calculate the concentrations of Aβ in the tissue samples, which were expressed as nmol of Aβ per g of wet brain tissue. The total CNBr cleavage yield as well as the yield from the plaque core preparation procedure as established previously were taken into account in the calculations.14

HPLC-MS/MS

The samples were injected into a high-capacity enrichment column chip with a 160-nl enrichment column and a 75 μm × 150 mm analytical column packed with Zorbax C18-SB 5-μm material (Agilent Technologies, Santa Clara, CA, USA). As described before,14 peptides were eluted using a water/acetonitrile (ACN) gradient supplemented with 0.2% FA, from 2 to 14% ACN in 20 min and from 14 to 95% ACN in 20 min. The flow rate was 0.4 μl/min, delivered by an Agilent 1200 nano pump (Agilent Technologies). The column chip was coupled to an Agilent ion trap mass spectrometer (6330 Ion Trap LC/MS, Agilent Technologies). Mass spectra were recorded from the mass to charge ratio (m/z 420 to m/z 750). The identity was confirmed using MS/MS data.

Fibroblast culture

Skin biopsy samples were taken and fibroblast cells were prepared as described.15 Fibroblast cell cultures from case III:1 were compared with selected cases, including an individual with the APP KM670/671NL Swedish mutation (APPSwe; onset age 58, biopsy at 66 years) and three unrelated, gender-matched and cell-passage-matched controls (mean age at biopsy: 43.7 years; range 26–53 years). Measurements of secreted and cellular Aβ40 and Aβ42 using ELISA provided the basis for comparison. The fibroblast cells were cultured in duplicate in 5 ml of minimum essential medium with Earl's salt (MEM), supplemented with 7.5% heat-inactivated fetal bovine serum, 10 m HEPES, 1.2% -glutamine, and 1.2% pencillin–streptomycin (all reagents from GIBCO, Invitrogen, Carlsbad, CA, USA). In parallel, fibroblast samples from the I143T, the APPSwe, and two of the controls were also cultured in the presence of the γ-secretase inhibitor L-685.485 (Bachem, Bubendorf, Switzerland) at a final concentration of 10 μ till the collection of the cell medium. When the cells reached ~100% confluency, the cell medium was replaced with serum-free MEM, supplemented with 10 m HEPES and 1.2% -glutamine, and incubated for 24 h, after which Complete protease inhibitor cocktail (Roche Applied Science) was added to the cell culture. The medium was removed, aliquoted in Genuine Axygen Quality 1.5-ml microtubes (Axygen, Union City, CA, USA), and stored at −80°C for future analysis. The remaining cells were treated with 1 ml of 1 × RIPA buffer (150 m NaCl, 1% NP-40, 0.5% deoxycholic acid, 0.1% SDS, 50 m Tris, pH 8) containing Complete protease inhibitor cocktail (Roche Applied Science), and harvested by scraping on ice. The cells were collected in Genuine Axygen Quality 1.5-ml microtubes (Axygen) and centrifuged at 25 000 g for 1 h at 4°C. The cell pellet and the supernatant (sup1) were stored at −80°C for future analysis.

ELISA

Aβ40 and Aβ42 secreted into the cell medium, and cellular (RIPA-soluble) Aβ40 and Aβ42 were measured in triplicate using ELISA (Human/Rat β-Amyloid (40) ELISA Kit Wako II and Human/Rat β-Amyloid (42) ELISA Kit Wako, High-Sensitive from WakoPure Chemical Industries, Osaka, Japan). As the levels of secreted Aβ40 were below the detection limit for the Wako kit, secreted Aβ1–40 in the medium was instead analyzed using the Human Amyloid-β (1–40) (FL) Assay Kit–IBL (IBL International GmbH, Hamburg, Germany). FLUOStar Galaxy (Thermo Scientific, Fremont, CA, USA) was used to measure the fluorescence. Before the cell medium samples and standards were loaded on the ELISA plate, 5 × RIPA buffer was added to a final concentration of 1 × RIPA buffer. In all, 200 μl of 1 × RIPA buffer was added to the cell pellet and sonicated for 10 min, followed by centrifugation at 25 000 g for 1 h at 4°C, generating supernatant 2 (sup 2). Sup 1 and 2 were pooled; the pooled samples thus contained the 1 × RIPA-soluble cellular Aβ, before loading onto the ELISA plate. The ELISA procedure was according to the manufacturer's protocol, except that TMB was exchanged with a 1 × PBS solution containing 50 μ Amplex UltraRed reagent (Invitrogen, Eugene, OR, USA), and 2.2 m H2O2 was added to each well before measuring the fluorescence, which was done using a 544 nm excitation filter and 590 nm emission filter, thus effectively increasing the sensitivity. Concentrations of Aβ40 and Aβ42 secreted into the cell medium and RIPA-soluble cellular samples (sup 1 and sup 2) were calculated from standard curves. The mean Aβ concentration was then normalized against the total cellular protein concentration measured using the Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, IL, USA) according to the manufacturer's protocol.

Results

Clinical characteristics

In a Swedish family, five family members across three generations were affected with pre-senile dementia (Figure 1). The age of onset was 35.6±2.1 years (mean±SD) and age at the first AD-related medical visit was 36.2±1.9 years. The mean age of death was 42.4±1.3 years and the duration of the disease from onset to death was 6.8±2.4 years. Initial symptoms were typical for AD and included memory impairment, visuospatial difficulties, disorientation, dyspraxia, and dysphasia. However, these symptoms were not always reported until the patient was admitted to hospital in acute condition, owing to falls or epileptic seizures. Additional early findings were coordination difficulties associated with gradually increasing myoclonic jerks, multiple falls, and, in some cases, epileptic seizures. These neurological symptoms made differential diagnosis difficult, specifically between AD and Huntington's disease. The latter diagnosis was excluded, however, by genetic testing. In the later stages of the disease, psychiatric symptoms such as paranoid delusions, hallucinations, and aggressiveness appeared and, in the first generation, Pick's disease was suspected owing to the observed personality changes. Within the youngest generation, cholinesterase inhibitor treatment was tried but did not seem to slow down the rapid progression of the disease. Anti-epileptic treatment seemed to reduce myoclonic jerks. For an overview of the clinical presentation, see Supplementary Table 1, and for a detailed description of the symptoms, clinical course, and treatment of the family members, see Supplementary Text 1.

Figure 1
Pedigree of the EOAD family with a PSEN1 I143T mutation. Filled symbols indicate affected individuals and symbols with a diagonal line indicate deceased individuals. Age at onset and age at death are shown below the symbols in years. The proband is indicated ...

Neuropathological examination

Brains from three mutation carriers (cases II:2, III:2, and III:1) were subjected to autopsy. A definite diagnosis of AD was established for cases III:1 and III:2 according to the CERAD criteria, and Braak stage VI. Case II:2 was diagnosed as atypical AD in 1985, owing to the prominence of pathology associated with the corpus striatum, which are not typically seen in AD cases. However, a recent re-examination revealed severe AD pathology. In summary, all brains were severely affected by Alzheimer's pathology and atrophy. Macroscopically, the frontal lobes were especially affected by the thinning of gyri and the reduced cortical thickness, whereas microscopically a large number of plaques were observed, often without a distinct plaque core. Tau pathology, including numerous ghost tangles, was widespread and severe in the hippocampal areas, entorhinal cortex, and amygdala. Superficial spongiform changes and gliosis were present. Interestingly, some neurons in the granular cell layer of the dentate gyrus were immunoreactive for the AT8 antibody, which recognizes the phosphorylated tau. The cerebellum, which often is relatively spared in AD, was clearly affected by amyloid in cases III:1 and III:2. For a detailed neuropathological description, see Supplementary Text 2.

Molecular genetics – identification of the pathogenic mutation

The family showed a clear pattern of dominantly inherited neurodegenerative disease; the neuropathological investigation of case III:2 prompted us to suspect early-onset AD and to screen for a mutation in PSEN1. A heterozygous point mutation was found in the second base in codon 143 of exon 5 in PSEN1, which results in an amino-acid exchange of isoleucine to threonine in the second transmembrane region of the PSEN1 protein. Restriction enzyme cleavage of the exon 5 PCR fragment using Tsp45 I resulted in two extra and shorter bands in addition to the 300-bp-long wild-type fragment, thereby confirming the presence of the I143T mutation (data not shown). The mutation was detected in genomic DNA from each of the three cases: II:2, III:1, and III:2 (Figure 1). Moreover, one variation was found in intron 8 (rs165932), which has previously been studied (http://www.alzgene.org/meta.asp?geneID=84). This variation is a common polymorphism and should not be considered as informative. No other genetic variation was found. Cases II:2, III:1, and III:2 were all APOE epsilon3epsilon3 homozygotes.

Analysis of Aβ C-terminal fragments in plaque cores and SDS-insoluble material

By using LC-MS, we quantified the amounts of the different Aβ C-terminal variants in plaque cores and the total amyloid SDS-insoluble material from frontal, parietal, temporal, and occipital cortex samples, as well as from the anterior hippocampus and cerebellum of two PSEN1 I143T mutation carriers (cases III:1 and III:2; Figure 1). Standard curves were plotted and used for calculations of the Aβ concentrations (Supplementary Figures S1A, S1B, and S1C, Supplementary Data). In plaque cores, Aβx-42 was readily detected in all regions from both patients. The lowest concentration was found in the cerebellum and the highest in the occipital cortex. Aβx-43 was found in all regions from both cases, except for the cerebellum, where it was not detected. In both cases, the highest concentration was found in the occipital cortex. Interestingly, Aβx-40 was not detected in any of the regions obtained from the two cases (Figure 2a).

Figure 2
Quantification of Aβ species in plaque cores and SDS-insoluble preparations from frontal, parietal, temporal, and occipital cortex, the anterior hippocampus and cerebellum of two carriers of PSEN1 mutation. (a) Plaque cores from six brain regions ...

We also analyzed total Aβ in SDS-insoluble material from brain homogenates. Aβx-42 was readily detected in all regions in both patients. The lowest concentration of Aβx-42 was found in the cerebellum, whereas the highest concentration was found in the occipital cortex. Aβx-43 was readily detected in all regions from both cases (Figure 2b). Interestingly, Aβx-40 was detected only in the occipital cortex and the cerebellum of case III:2.

Immunohistochemistry

Antibodies specific for the C-terminus of Aβx-40, Aβx-42, or Aβx-43 were used to stain consecutive sections taken from the six regions of the two cases. The staining of Aβx-40 was low in the occipital cortex in both cases, and was only detected in vessels (Figure 3a and d), whereas Aβx-42 staining was intense in both cases, with numerous immunoreactive plaques (Figure 3b and e). Many immunoreactive plaques were also evident by the Aβx-43 antibody (Figure 3c and f). Although the methodology used does not allow for quantification and co-localization of the immunoreactivity, visual inspection in the microscope revealed that Aβx-42 and Aβx-43 immunoreactivity was detected in both plaques and vessels, whereas Aβx-40 showed immunoreactivity mainly in vessels (Figure 3g, h and i). In the cerebellum, Aβx-40 staining was clearly visible only in a few vessels, most prominently in case III:2 (Figure 3j and m). Aβx-42 staining was observed in vessels in both cases (Figure 3k and n). Aβx-43 staining was positive in vessels and the pattern was similar to the Aβx-42 staining in both cases (Figure 3l and o). Moreover, extracellular Aβx-42 and Aβx-43 staining was also observed in an uneven and diffuse pattern in the molecular layer.

Figure 3
Immunohistochemistry. Immunostaining of amyloid in plaques and vessels in the occipital cortex (ai) and cerebellum (jo) from case III:2 and case III:1. In each case, three consecutive sections were stained with C-terminal antibodies ...

The immune staining of the frontal, temporal, and parietal cortex, as well as hippocampus, showed little or no Aβx-40 immunoreactivity, and when present, it was only observed in vessels, a pattern seen also in the occipital cortex and the cerebellum. Aβx-42 was positive in both plaques and vessels, and Aβx-43 seemed to have a similar staining pattern (data not shown) in these four regions.

Aβ production in cultured fibroblasts from mutation carriers and controls

As a complement, a sensitive ELISA was implemented to measure Aβ production in cultured fibroblasts. Results showed that secreted Aβ40 levels were low in both the PSEN1 I143T mutation carrier (case III:1) and the healthy controls (<5 pmol per g total protein), although the level was five times higher in the APPSwe carrier (~25 pmol per g total protein). Secreted Aβ42 levels were also low (≤1 pmol per g total protein) and equal in all samples, except the APPSwe mutation carrier, in which the concentration was about three times higher (2.8 pmol per g total protein). In the presence of the γ-secretase inhibitor, L-685.485, no Aβ was detected (data not shown). The ratio of secreted Aβ42/40 was highest in the PSEN1 I143T carrier (0.5; Figure 4a).

Figure 4
Secreted and cellular Aβ40 and Aβ42 concentrations in cultured fibroblasts. (a) Secreted and (b) cellular Aβ40 and Aβ42 levels from cultured fibroblasts measured by ELISA in a PSEN1 I143T mutation carrier, three healthy ...

The cellular Aβ40 concentration was equivalent in all samples (40–55 pmol per g total protein) and significantly higher when compared with secreted Aβ40 (about two times higher in the APPSwe carrier, and up to 50 times higher in the PSEN1 I143T mutation carrier and the healthy controls). The cellular Aβ42 concentration was higher across all samples (~8 pmol per g total protein) than the secreted Aβ42 concentration (0.5–2.8 pmol per g total protein). Therefore, the cellular ratio of Aβ42/40 was similar (0.14–0.16) across all samples (Figure 4b). No significant inhibition of Aβ was detected in the presence of the γ-secretase inhibitor, L-685.485 (data not shown).

Discussion

In the present study, we identified a PSEN1 I143T mutation in three cases diagnosed with neurodegenerative disease from two generations in a Swedish family. This mutation has previously been reported to cause EOAD both in single unrelated cases, related cases and in two families in which segregation was proven, but clinical, neuropathological, and biochemical details have been lacking.16, 17, 18, 19, 20, 21, 22, 23 The form of EOAD recurring in this family is severe and its mean onset age is reported to be 34 years, whereas the mean age of death is 41.2 years (http://www.molgen.ua.ac.be/ADMutations). Our clinical investigation revealed an aggressive form of AD with rapid progression and extensive myoclonia. Among the clinical characteristics shown in this Swedish family were insidious impairment of memory, visuospatial disorientation, dyspraxia, and dysphasia, as well as coordination problems, including myoclonic jerks and multiple falls, and in some cases epileptic seizures. The clinical characteristics of the Belgian family with the same mutation, described as early as in 1940 as ‘une observation hérédo-familiale de démence avec épilepsie et clonismes',16, 18, 24 were similar to the characteristics of the Swedish family in terms of age at onset, increased aggressiveness, rapid loss of memory, myoclonus, and seizures.

We performed quantitative and qualitative biochemical studies of Aβ pathology on six brain regions sampled from two cousins known to have the I143T mutation. Interestingly, we found that a long Aβ species, ending with Thr43, was present in plaque cores in all regions except in cerebellum. In all the analyzed regions, Aβx-43 was found more frequently than Aβx-40, as shown by both mass spectrometry analysis and immunohistochemistry. The highest concentrations of both Aβx-42 and Aβx-43 were found in the occipital cortex from both mutation carriers. This supports the notion that the occipital cortex is a plaque core-rich region.25 Even though immunostaining in both cases showed that the cerebellum was affected by Aβ pathology, the lowest plaque core concentration of Aβx-42 was found in preparations from this area, and moreover, Aβx-43 was not detected by mass spectrometry. The concentrations of Aβx-42 were similar in the frontal cortex and anterior hippocampus. The levels were higher in both the parietal and temporal cortex than in either the frontal cortex or anterior hippocampus. There were no significant differences in the concentration of Aβx-43 between these regions. In accordance with earlier studies,6 we conclude that Aβ42 is the predominant species in amyloid plaque cores in the AD brain. Notably, there were no detectable levels of Aβx-40 in plaque cores, which is in line with previous results.6

As Aβ is deposited not only in plaque cores in the AD brain, but also in the walls of the cerebral vessels as diffuse deposits, we studied the total Aβ from the two cases by analyzing Aβ peptides in the material from SDS-insoluble preparations. Aβx-42 and Aβx-43 were readily detected in all regions. The concentrations were highest in the occipital cortex and lowest in the cerebellum. Interestingly, there were no detectable levels of Aβx-40 in the SDS-insoluble preparations except in the cerebellum and the occipital cortex of case III:2. The levels of Aβx-40 in these regions most likely stem from amyloid in cerebral vessels associated with cerebral amyloid angiopathy, which is reported to be composed mainly of Aβx-40.6, 26 As Aβx-40 has a lower aggregation propensity compared with the longer Aβ species, differences in Aβx-40 levels between the cases might be explained by the longer duration of the disease in case III:2, as compared with case III:1. Mass spectrometry data are also supported by the immunohistochemical analysis of the six regions, which showed that Aβx-40 was localized mainly to the cerebral vessels, whereas Aβx-42 and Aβx-43 were more prominent in plaques.

Using ELISA, we measured the concentrations of cellular and secreted Aβ40 and Aβ42 in cultured fibroblasts sampled from a PSEN1 I143T mutation carrier, an APPSwe mutation carrier, and three healthy controls. The APPSwe mutation carrier differed notably, with higher concentrations of secreted Aβ40 and Aβ42 compared with all other samples. Cellular Aβ40 and Aβ42 concentrations did not differ significantly between the samples and the levels of Aβ42 were lower in comparison with Aβ40. The highest ratio of Aβ42/40 was observed in the secreted fraction from the I143T mutation carrier, in line with previous studies on PSEN1 mutations.8, 27 Although the secreted Aβ was effectively inhibited in the presence of a γ-secretase inhibitor, the inhibition was not effective with respect to the cellular levels of Aβ. We do not know why the cellular levels of Aβ were not reduced by the γ-secretase inhibitor treatment, but one explanation might be the inability of the inhibitor to enter the human fibroblast cells.

The neurological manifestations in this family are also prevalent in other EOAD28 cases and is considered as secondary to the neurodegenerative process. In the described family, all patients had some neurological dysfunctions, such as falls, myoclonus, or epileptic seizures. Experimental data indicate that high levels of Aβ in hAPP transgenic mouse brains might cause epileptic activity and contribute to cognitive decline.28, 29 We showed that the PSEN1 mutation carriers had severe Aβ pathology, using both immunohistochemical staining and mass spectrometry analysis, which might have triggered the myoclonus and epileptic manifestations.

As the Aβ43 peptide is more hydrophobic than Aβ42, and as longer forms polymerize faster and at lower concentrations than the more abundant shorter variants, it is plausible that the Aβ43 isoform also forms toxic oligomers to a greater extent than Aβ40 and Aβ42 in vivo. However, in postmortem studies, such as this one, initiating events of the disease cannot be observed. As Aβ oligomers seem to be of particular importance as neurotoxins, further studies on the presence of Aβ43 oligomers are warranted. Furthermore, in vivo and in vitro studies focusing on Aβ43 are encouraged, in particular with respect to the design of Aβ vaccination trials.

In summary, we identified an I143T PSEN1 mutation in a Swedish family, which is the sixth AD mutation found in Sweden that causes an aggressive form of EOAD. We also found a longer Aβ species, Aβ43, which was present in each examined brain area, except for the cerebellum, which was, on the other hand, more extensively affected by Aβ40. Although the absolute levels of Aβ43 were low, it was clearly more frequent than Aβ40, thus indicating that Aβ43 has an important role in the disease process of AD.

Acknowledgments

We express our sincere gratitude to the members of the family in our study. We are also thankful to Masakazu Hashimoto for sharing his knowledge about ELISA techniques. This study was financially supported by the Alzheimer Foundation, Foundation for Old Servants, Gun and Bertil Stohne's Foundation, Swedish Brain Power, Marianne and Marcus Wallenberg Foundation, Knut and Alice Wallenberg Foundation, and Dainippon Sumitomo Pharma (Japan).

Notes

The authors declare no conflict of interest.

Footnotes

Supplementary Information accompanies the paper on European Journal of Human Genetics website (http://www.nature.com/ejhg)

Supplementary Material

Supplementary Figures 1A–C

Supplementary Text 1

Supplementary Text 2

Supplementary Table 1

Supplementary Data

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