• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Nov 1989; 86(22): 8988–8992.
PMCID: PMC298417

Recruitment of a penicillin-binding protein gene from Neisseria flavescens during the emergence of penicillin resistance in Neisseria meningitidis.

Abstract

Non-beta-lactamase-producing, penicillin-resistant strains of Neisseria meningitidis produce altered forms of penicillin-binding protein 2 that have decreased affinity for penicillin. The sequence of the penicillin-binding protein 2 gene (penA) from a penicillin-resistant strain of N. meningitidis was compared to the sequence of the same gene from penicillin-sensitive strains and from penicillin-sensitive and penicillin-resistant strains of Neisseria gonorrhoeae. The penA genes from penicillin-sensitive strains of N. gonorrhoeae and N. meningitidis were 98% identical. The gene from the penicillin-resistant strain of N. meningitidis consisted of regions that were almost identical to the corresponding regions in the penicillin-sensitive strains (less than 0.2% divergence) and two regions that were very different from them (approximately 22% divergence). The two blocks of altered sequence have arisen by the replacement of meningococcal sequences with the corresponding regions from the penA gene of Neisseria flavescens and result in an altered form of penicillin-binding protein 2 that contains 44 amino acid substitutions and 1 amino acid insertion compared to penicillin-binding protein 2 of penicillin-sensitive strains of N. meningitidis. A similar introduction of part of the penA gene of N. flavescens, or a very similar commensal Neisseria species, appears to have occurred independently during the development of altered penA genes in non-beta-lactamase-producing penicillin-resistant strains of N. gonorrhoeae.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Jephcott AE. Epidemiology of resistance in Neisseria gonorrhoeae. J Antimicrob Chemother. 1986 Oct;18 (Suppl 100):199–205. [PubMed]
  • Dougherty TJ, Koller AE, Tomasz A. Penicillin-binding proteins of penicillin-susceptible and intrinsically resistant Neisseria gonorrhoeae. Antimicrob Agents Chemother. 1980 Nov;18(5):730–737. [PMC free article] [PubMed]
  • Faruki H, Sparling PF. Genetics of resistance in a non-beta-lactamase-producing gonococcus with relatively high-level penicillin resistance. Antimicrob Agents Chemother. 1986 Dec;30(6):856–860. [PMC free article] [PubMed]
  • Dillon JR, Pauzé M, Yeung KH. Spread of penicillinase-producing and transfer plasmids from the gonococcus to Neisseria meningitidis. Lancet. 1983 Apr 9;1(8328):779–781. [PubMed]
  • Sáez-Nieto JA, Fontanals D, Garcia de Jalon J, Martinez de Artola V, Peña P, Morera MA, Verdaguer R, Sanfeliu I, Belio-Blasco C, Perez-Saenz JL, et al. Isolation of Neisseria meningitidis strains with increase of penicillin minimal inhibitory concentrations. Epidemiol Infect. 1987 Oct;99(2):463–469. [PMC free article] [PubMed]
  • Botha P. Penicillin-resistant Neisseria meningitidis in southern Africa. Lancet. 1988 Jan 2;1(8575-6):54–54. [PubMed]
  • Sutcliffe EM, Jones DM, el-Sheikh S, Percival A. Penicillin-insensitive meningococci in the UK. Lancet. 1988 Mar 19;1(8586):657–658. [PubMed]
  • Fontanals D, Pineda V, Pons I, Rojo JC. Penicillin-resistant beta-lactamase producing Neisseria meningitidis in Spain. Eur J Clin Microbiol Infect Dis. 1989 Jan;8(1):90–91. [PubMed]
  • Mendelman PM, Campos J, Chaffin DO, Serfass DA, Smith AL, Sáez-Nieto JA. Relative penicillin G resistance in Neisseria meningitidis and reduced affinity of penicillin-binding protein 3. Antimicrob Agents Chemother. 1988 May;32(5):706–709. [PMC free article] [PubMed]
  • Mendelman PM, Caugant DA, Kalaitzoglou G, Wedege E, Chaffin DO, Campos J, Saez-Nieto JA, Viñas M, Selander RK. Genetic diversity of penicillin G-resistant Neisseria meningitidis from Spain. Infect Immun. 1989 Apr;57(4):1025–1029. [PMC free article] [PubMed]
  • Spratt BG. Hybrid penicillin-binding proteins in penicillin-resistant strains of Neisseria gonorrhoeae. Nature. 1988 Mar 10;332(6160):173–176. [PubMed]
  • Dowson CG, Jephcott AE, Gough KR, Spratt BG. Penicillin-binding protein 2 genes of non-beta-lactamase-producing, penicillin-resistant strains of Neisseria gonorrhoeae. Mol Microbiol. 1989 Jan;3(1):35–41. [PubMed]
  • Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. [PubMed]
  • Dowson CG, Hutchison A, Spratt BG. Extensive re-modelling of the transpeptidase domain of penicillin-binding protein 2B of a penicillin-resistant South African isolate of Streptococcus pneumoniae. Mol Microbiol. 1989 Jan;3(1):95–102. [PubMed]
  • Dowson CG, Hutchison A, Brannigan JA, George RC, Hansman D, Liñares J, Tomasz A, Smith JM, Spratt BG. Horizontal transfer of penicillin-binding protein genes in penicillin-resistant clinical isolates of Streptococcus pneumoniae. Proc Natl Acad Sci U S A. 1989 Nov;86(22):8842–8846. [PMC free article] [PubMed]
  • Hedge PJ, Spratt BG. Resistance to beta-lactam antibiotics by re-modelling the active site of an E. coli penicillin-binding protein. Nature. 1985 Dec 5;318(6045):478–480. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...