• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Nov 1989; 86(22): 8793–8797.
PMCID: PMC298376

Stimulation of the expression of osteogenic and chondrogenic phenotypes in vitro by osteogenin.

Abstract

Osteogenin was recently purified and the amino acid sequences of tryptic peptides were determined. Osteogenin in conjunction with insoluble collagenous bone matrix induces cartilage and bone formation in vivo. To understand the mechanism of action of osteogenin, we examined its influence on periosteal cells, osteoblasts, fibroblasts, chondrocytes, and bone marrow stromal cells in vitro. Osteogenin stimulated alkaline phosphatase activity and collagen synthesis in periosteal cells. The cAMP response to parathyroid hormone in periosteal cells was increased by osteogenin. In primary cultures of calvarial osteoblasts, osteogenin stimulated alkaline phosphatase activity, the cAMP response to parathyroid hormone, and the synthesis of collagenous and noncollagenous proteins; however, cell proliferation was not affected. Osteogenin increased the production of sulfated proteoglycans in fetal rat chondroblasts and in rabbit articular chondrocytes. The present experiments demonstrate the significant influence of osteogenin in the stimulation of osteogenic and chondrogenic phenotypes in vitro.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Urist MR. Bone: formation by autoinduction. Science. 1965 Nov 12;150(3698):893–899. [PubMed]
  • Reddi AH, Huggins C. Biochemical sequences in the transformation of normal fibroblasts in adolescent rats. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1601–1605. [PMC free article] [PubMed]
  • Reddi AH, Anderson WA. Collagenous bone matrix-induced endochondral ossification hemopoiesis. J Cell Biol. 1976 Jun;69(3):557–572. [PMC free article] [PubMed]
  • Reddi AH, Huggins CB. Hormone-dependent haematopoiesis in fibroblast-transformation ossicles. Nature. 1976 Oct 7;263(5577):514–515. [PubMed]
  • Rath NC, Reddi AH. Collagenous bone matrix is a local mitogen. Nature. 1979 Apr 26;278(5707):855–857. [PubMed]
  • Sampath TK, Muthukumaran N, Reddi AH. Isolation of osteogenin, an extracellular matrix-associated, bone-inductive protein, by heparin affinity chromatography. Proc Natl Acad Sci U S A. 1987 Oct;84(20):7109–7113. [PMC free article] [PubMed]
  • Luyten FP, Cunningham NS, Ma S, Muthukumaran N, Hammonds RG, Nevins WB, Woods WI, Reddi AH. Purification and partial amino acid sequence of osteogenin, a protein initiating bone differentiation. J Biol Chem. 1989 Aug 15;264(23):13377–13380. [PubMed]
  • Wozney JM, Rosen V, Celeste AJ, Mitsock LM, Whitters MJ, Kriz RW, Hewick RM, Wang EA. Novel regulators of bone formation: molecular clones and activities. Science. 1988 Dec 16;242(4885):1528–1534. [PubMed]
  • Muthukumaran N, Ma S, Reddi AH. Dose-dependence of and threshold for optimal bone induction by collagenous bone matrix and osteogenin-enriched fraction. Coll Relat Res. 1988 Sep;8(5):433–441. [PubMed]
  • Wong GL, Cohn DV. Target cells in bone for parathormone and calcitonin are different: enrichment for each cell type by sequential digestion of mouse calvaria and selective adhesion to polymeric surfaces. Proc Natl Acad Sci U S A. 1975 Aug;72(8):3167–3171. [PMC free article] [PubMed]
  • Hefley T, Cushing J, Brand JS. Enzymatic isolation of cells from bone: cytotoxic enzymes of bacterial collagenase. Am J Physiol. 1981 May;240(5):C234–C238. [PubMed]
  • McCarthy TL, Centrella M, Canalis E. Further biochemical and molecular characterization of primary rat parietal bone cell cultures. J Bone Miner Res. 1988 Aug;3(4):401–408. [PubMed]
  • Nijweide PJ, van der Plas A, Scherft JP. Biochemical and histological studies on various bone cell preparations. Calcif Tissue Int. 1981;33(5):529–540. [PubMed]
  • Owen ME, Cavé J, Joyner CJ. Clonal analysis in vitro of osteogenic differentiation of marrow CFU-F. J Cell Sci. 1987 Jun;87(Pt 5):731–738. [PubMed]
  • Friedenstein AJ, Chailakhyan RK, Gerasimov UV. Bone marrow osteogenic stem cells: in vitro cultivation and transplantation in diffusion chambers. Cell Tissue Kinet. 1987 May;20(3):263–272. [PubMed]
  • Zanetti NC, Solursh M. Induction of chondrogenesis in limb mesenchymal cultures by disruption of the actin cytoskeleton. J Cell Biol. 1984 Jul;99(1 Pt 1):115–123. [PMC free article] [PubMed]
  • Labarca C, Paigen K. A simple, rapid, and sensitive DNA assay procedure. Anal Biochem. 1980 Mar 1;102(2):344–352. [PubMed]
  • Luyten FP, Hascall VC, Nissley SP, Morales TI, Reddi AH. Insulin-like growth factors maintain steady-state metabolism of proteoglycans in bovine articular cartilage explants. Arch Biochem Biophys. 1988 Dec;267(2):416–425. [PubMed]
  • Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983 Dec 16;65(1-2):55–63. [PubMed]
  • McCarthy TL, Centrella M, Canalis E. Regulatory effects of insulin-like growth factors I and II on bone collagen synthesis in rat calvarial cultures. Endocrinology. 1989 Jan;124(1):301–309. [PubMed]
  • Peterkofsky B, Diegelmann R. Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteins. Biochemistry. 1971 Mar 16;10(6):988–994. [PubMed]
  • Yanagishita M, Midura RJ, Hascall VC. Proteoglycans: isolation and purification from tissue cultures. Methods Enzymol. 1987;138:279–289. [PubMed]
  • Majeska RJ, Rodan GA. Alkaline phosphatase inhibition by parathyroid hormone and isoproterenol in a clonal rat osteosarcoma cell line. Possible mediation by cyclic AMP. Calcif Tissue Int. 1982 Jan;34(1):59–66. [PubMed]
  • Rodan SB, Wesolowski G, Thomas K, Rodan GA. Growth stimulation of rat calvaria osteoblastic cells by acidic fibroblast growth factor. Endocrinology. 1987 Dec;121(6):1917–1923. [PubMed]
  • Vukicević S, Marusić A, Stavljenić A, Cicak N, Vogel M, Krempien B. Talc granulomatosis in the rat: the relationship between osteoblast insufficiency and adjacent bone marrow hyperplasia. Exp Hematol. 1988 Oct;16(9):735–740. [PubMed]
  • Rosen DM, Stempien SA, Thompson AY, Seyedin SM. Transforming growth factor-beta modulates the expression of osteoblast and chondroblast phenotypes in vitro. J Cell Physiol. 1988 Mar;134(3):337–346. [PubMed]
  • Włodarski KH. Normal and heterotopic periosteum. Clin Orthop Relat Res. 1989 Apr;(241):265–277. [PubMed]
  • Scott BL. Thymidine-3H electron microscope radioautography of osteogenic cells in the fetal rat. J Cell Biol. 1967 Oct;35(1):115–126. [PMC free article] [PubMed]
  • Miller SC, Bowman BM. Medullary bone osteogenesis following estrogen administration to mature male Japanese quail. Dev Biol. 1981 Oct 15;87(1):52–63. [PubMed]
  • Nijweide PJ, Mulder RJ. Identification of osteocytes in osteoblast-like cell cultures using a monoclonal antibody specifically directed against osteocytes. Histochemistry. 1986;84(4-6):342–347. [PubMed]
  • Nijweide PJ, van Iperen-van Gent AS, Kawilarang-de Haas EW, van der Plas A, Wassenaar AM. Bone formation and calcification by isolated osteoblastlike cells. J Cell Biol. 1982 May;93(2):318–323. [PMC free article] [PubMed]
  • Ecarot-Charrier B, Glorieux FH, van der Rest M, Pereira G. Osteoblasts isolated from mouse calvaria initiate matrix mineralization in culture. J Cell Biol. 1983 Mar;96(3):639–643. [PMC free article] [PubMed]
  • Bellows CG, Aubin JE, Heersche JN, Antosz ME. Mineralized bone nodules formed in vitro from enzymatically released rat calvaria cell populations. Calcif Tissue Int. 1986 Mar;38(3):143–154. [PubMed]
  • Gerstenfeld LC, Chipman SD, Kelly CM, Hodgens KJ, Lee DD, Landis WJ. Collagen expression, ultrastructural assembly, and mineralization in cultures of chicken embryo osteoblasts. J Cell Biol. 1988 Mar;106(3):979–989. [PMC free article] [PubMed]
  • Simmons DJ, Kent GN, Jilka RL, Scott DM, Fallon M, Cohn DV. Formation of bone by isolated, cultured osteoblasts in millipore diffusion chambers. Calcif Tissue Int. 1982 May;34(3):291–294. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...