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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Vaccine. Author manuscript; available in PMC Nov 10, 2011.
Published in final edited form as:
PMCID: PMC2981674
NIHMSID: NIHMS236319

The Association of Ethnicity with Antibody Responses to Pneumococcal Vaccination among Adults with HIV Infection

Nancy F. Crum-Cianflone, MD MPH,a,b Mollie Roediger, MS,a,c Kathy Huppler Hullsiek, PhD,a,c Anuradha Ganesan, MD,a,d Michael Landrum, MD,a,e Amy Weintrob, MD,a,f Brian Agan, MD,a Sheila Medina, MPH,a,b Jeremy Rahkola,g Braden Hale, MD MPH,a,h and Edward N. Janoff, MDg, the Infectious Disease Clinical Research Program HIV Working Group

Abstract

Ethnicity may be associated with the incidence of pneumococcal infections and the frequency of protective vaccine responses. Earlier studies have suggested that HIV-infected persons of black ethnicity develop less robust immune responses to pneumococcal vaccination that may relate to their higher incidence of invasive disease. We evaluated the association of ethnicity with capsule-specific antibody responses to pneumococcal revaccination, with either the pneumococcal conjugate (PCV) or polysaccharide (PPV) vaccines among 188 HIV-infected adults. The proportion of the 77 African Americans (AA) and 111 Caucasians with comparable virologic and immunologic parameters who achieved a positive immune response (≥2-fold rise in capsule-specific IgG from baseline with post-vaccination value ≥1 µg/mL for ≥2 of 4 serotypes) at day 60 after revaccination was similar (43% vs. 49%, respectively, p=0.65). Results were also similar when vaccine types (PPV and PCV) were examined separately. Mean changes in log10 transformed IgG levels from baseline to days 60 and 180 post-vaccination were also not significantly different between AA and Caucasians. In summary, in this ethnically diverse cohort with equal access to care, we did not observe differential antibody responses between AA and Caucasian HIV-infected adults after pneumococcal revaccination.

1. Introduction

Streptococcus pneumoniae infections are a common cause of morbidity among persons infected with the human immunodeficiency virus (HIV) [17]. Several studies have demonstrated an ethnic disparity among rates of pneumococcal disease with an increased risk among blacks compared with whites in both the general population and persons infected with HIV [3, 812].

The efficacy of pneumococcal vaccinations in preventing invasive pneumococcal disease among HIV-infected adults is suboptimal in all ethnic groups [6]. A limited IgG antibody response to pneumococcal capsular polysaccharides, an important determinant of disease and protection, among black Americans and Africans has been proposed to contribute to the higher risk of disease in this ethnic group [12, 13]. However, the exact nature of this proposed poor vaccine efficacy is unclear as little data are available that directly compare antibody levels generated post-vaccination among HIV-infected persons of differing ethnicities. Therefore, we utilized data from a prospective, randomized study to compare capsule-specific IgG levels prior to and following pneumococcal revaccination in African American (AA) and Caucasian HIV-infected adults.

2. Methods

2.1. Study Population

We performed subgroup analyses of capsule-specific IgG responses among AA and Caucasians from a randomized study comparing the immunogenicity of revaccination with pneumococcal conjugate vaccine (PCV) to pneumococcal polysaccharide vaccine (PPV) among HIV-infected adults previously vaccinated with PPV. The main study evaluated 204 HIV-infected adults who were randomized (2:1) to PCV (Prevnar; Wyeth Pharmaceuticals, n=131) or PPV (Pneumovax, Merck & Co., Inc., n=73) between February 2006 and September 2008 [14].

Of all study participants, 77 were AA and 111 Caucasians, and these subjects are the focus of this sub-analysis. Data on ethnicity was based on self report. Study participants were infected with HIV (documented by a positive ELISA with Western Blot confirmation), between ages 18–60 years, had received a prior PPV vaccination 3–8 years earlier, and without significant concurrent medical conditions except for HIV infection. All participants were military beneficiaries who have open and free access to healthcare, and low rates of illicit drug use [15]. Study subjects provided written informed consent, and the study was approved by the governing institutional review boards and registered with the Clinical Trials network (registration NCT00622843).

2.2. Study Design and Procedures

The primary study outcome was achieving a positive immune response, defined as a ≥2-fold rise in capsule-specific IgG with post-vaccination value ≥1 µg/mL, at day 60 post-vaccination for at least 2 of 4 serotypes. The endpoint was chosen in concordance with prior reports, and a threshold value of 1 µg/mL was used to assure that fold rises represented meaningful post-vaccination antibody levels [16, 17]. Secondary outcomes included positive IgG responses and changes in capsule-specific IgG concentrations for each serotype at each time point.

Pneumococcal vaccines were administered intramuscularly (0.5 ml) in the deltoid muscle using a 23-gauge, 1-inch needle in accordance with manufacturers’ guidelines. Serum samples were collected at baseline (1–21 days prior to revaccination) and days 14, 60, and 180 after revaccination. We determined the capsule specific IgG levels to four pneumococcal serotypes (4, 9V, 14, and 19F), which represented a range of important serotypes among HIV-associated pneumococcal infections.

2.3 Assays

Serotype-specific pneumococcal IgG concentrations were measured by ELISA, as previously described [14, 18]. In brief, sera were preadsorbed with 4 µg/mL of cell wall polysaccharide and 2 µg/mL of type 22F capsular polysaccharide overnight to eliminate non-capsule-specific antibodies [19, 20]. Capsular polysaccharides were adhered to 96-well microtiter plates, and capsule-specific IgG was detected with affinity-purified horseradish peroxidase-conjugated goat anti-human IgG label and appropriate substrates. Samples were tested in triplicate. Antibody concentrations on each plate were based on extrapolation from standard values on each plate (Standard Reference Serum 89-SF; provided by the Food and Drug Administration).

2.4. Data Analysis

Descriptive statistics are presented as means with standard deviations (SD) and medians with interquartile ranges (IQR). Proportions were compared between ethnic groups using Chi-square tests, and medians compared using Kruskal-Wallis tests. Odds ratios (OR) for achieving a positive immune response (AA vs. Caucasian) were generated with adjusted logistic regression models. Sensitivity analyses for the primary outcome included evaluating the effect of different post-vaccination threshold values. Secondary analyses evaluated changes from baseline in IgG concentrations, calculated after log10 transformation for AA and Caucasians for each serotype at each study time point, and were compared using adjusted generalized linear fixed-effects regression models. OR and change from baseline data are presented with 95% confidence intervals (CI). All logistic and linear regression models were adjusted for vaccine group (PCV vs. PPV), baseline type-specific IgG concentrations after log10 transformation, age (≤40 years vs. >40 years), prior pneumonia, CD4+ T cell number (<500 cells/mm3 vs. ≥500 cells/mm3), plasma HIV RNA level (≤50 copies vs. >50 copies/mL), and antiretroviral use at time of vaccination. All p-values are two sided and analyses were conducted using SAS (version 9.2, SAS Institute, Cary, North Carolina, USA).

3. Results

3.1. Study Population Characteristics

The median age of participants was 42 (IQR 36–47) years and 95% were males (Table 1). The median number of prior PPV immunizations were one, which was administered a median of 4.5 years prior to study enrollment. Eighteen percent of participants had a history of prior pneumonia. Regarding HIV history, the median duration of HIV infection was 9.5 (IQR 5–16) years, median CD4+ T cell count was 540 (IQR 391–701) cells/mm3, and 81% were receiving HAART. Of the 188 participants, 121 were randomized to be revaccinated with PCV and 67 were randomized to be revaccinated with PPV. There were no differences in baseline characteristics between AA and Caucasian participants, except that Caucasians were more likely to have a history of pneumonia (23% vs. 10%, p=0.03).

Table 1
Baseline Characteristics of Study Population by Ethnicity

3.2. Immune Responses to Pneumococcal Vaccination

Pre-vaccination antibody levels did not vary by ethnicity for any of the four serotypes (Table 2). The proportion of AA and Caucasians who achieved a positive immune response at day 60 after revaccination was similar (43% vs. 49%, respectively, p=0.65) (Figure 1). For IgG responses for individual serotypes, a lower proportion of AA generated responses for serotypes 9V and 14 compared with responses in Caucasians after revaccination at day 14. However, by days 60 and 180, there were no differences between the ethnic groups for any serotype (Figure 1). Results were similar for the primary endpoint when modifying the criterion from achieving a post-vaccination level of ≥1 µg/mL to achieving a level of ≥0 µg/mL, ≥0.35 µg/mL, or ≥5 µg/mL (data not shown).

Figure 1
Adjusted Odds Ratios for Achieving a Positive Immune Response* to Pneumococcal Revaccination for African Americans versus Caucasians
Table 2
Absolute IgG levels (µg/mL) at Baseline and at Days 14, 60, and 180 Post-Vaccination among African Americans and Caucasians

There were no significant differences in median unadjusted IgG levels (µg/mL) between Caucasians and AA for any serotype at any timepoint (Table 2). The adjusted mean changes in IgG concentration (expressed as log10 µg/mL) from baseline to day 60 or day 180 were also similar among Caucasians and AA for all serotypes (Figure 2). In addition, when the two pneumococcal vaccines (PCV and PPV) were examined separately for positive immune responses and changes in GMC at day 60, results showed no significant ethnic differences. Finally, we examined if a history of prior pneumonia was associated with antibody responses and found no significant associations (data not shown).

Figure 2
Adjusted Means (SE) of change in IgG Concentration (expressed as log10 µg/mL) from Baseline to Days 14, 60, and 180 by Serotype among African Americans versus Caucasians

4. Discussion

Vaccination is a central strategy for pneumococcal disease prevention and is advocated for high-risk groups, including persons with HIV infection [21]. As such, it is important to determine if ethnic disparities in pneumococcal disease incidence rates may be associated with differences in the immunogenicity and efficacy of vaccinations among ethnic groups. Two studies have shown that the administration of PPV among HIV-infected black Americans [12] and Africans [13] result in disproportionately limited efficacy, and may even be associated with detrimental effects [13]. The mechanisms underlying the poor vaccine efficacy among blacks in these studies is unclear. Immunogenicity studies among HIV-infected Africans have found suboptimal responses to PPV [22] and have correlated low post-vaccination capsule-specific IgG levels with clinical events [23]. Whether poor responses were due to advanced HIV disease, nutritional/environmental factors, or other ethnicity-associated factors remains unclear since those studies did not have a racially-diverse comparator group.

We investigated whether HIV-infected AA compared with Caucasians generated differential immune responses to pneumococcal vaccination. In our ethnically diverse HIV cohort with equal access to care, we did not observe differential post-vaccination immune responses at days 60 or 180 between AA and Caucasians to either vaccine preparation. Hence, our data suggest that previously described ethnic disparities in pneumococcal disease frequencies may not be associated with differences in vaccine immunogenicity, particularly when controlled for clinical and immune status as well as access to care. Indeed, a recent trial showed protection against invasive pneumococcal disease among black HIV-infected African adults with prior disease who received PCV [24]. Thus, African ethnicity alone may not be associated with poorer vaccine responses. Of note, our study had the advantage of directly comparing responses among AA and Caucasians in a comparable controlled setting, a strength compared with prior studies [13, 24].

The reasons for the higher rates of pneumococcal disease reported among blacks require further investigation [3, 812]. That one epidemiologic study found that blacks had a 5-fold higher rate of pneumococcal disease compared to whites, independent of socioeconomic status or population density [9, 10], suggests that population genetics may play a role. However, differences in socioeconomic status or crowding leading to varying healthcare access or other environmental factors may also contribute to these differential rates of disease. Understanding of the basis for previously described ethnic differences in pneumococcal disease incidences and responses to vaccination are especially important among HIV-infected persons, among whom the incidence of invasive pneumococcal disease is substantially increased [1, 4, 7]. Furthermore, the majority of the 33 million HIV-infected persons worldwide are of African descent [25, 26].

Our study had potential limitations. Our study did not determine IgM anti-pneumococcal responses which may be suboptimal among HIV-infected persons [27]. However IgM responses are typically transient and we focused on IgG responses which represent more durable responses that likely best correlate with pneumococcal protection over time. Furthermore, data on the functionality of the IgG antibodies as determined by opsonophagocytic assays or avidity are not currently available for our study cohort. Finally, although our randomized study comparing the immunogenicity of PCV vs. PPV revaccination was adequately powered [14], as a substudy, these analyses were not powered to detect differences by ethnicity. Nonetheless, we did not find any patterns for differences of durable antibody responses (at days 60 or 180) among the two ethnic groups.

Our U.S.-based study provides important clinical data by demonstrating that AA and Caucasians infected with HIV respond equally to pneumococcal revaccination. Since our population consisted of an early-diagnosed and treated U.S. HIV cohort, we recommend further studies examining pneumococcal capsule-specific IgG responses among various ethnic groups in other settings, including Africa. We recognize that AA participants in our study cohort likely come from diverse ethnic backgrounds in Africa, and that our study did not include genetic analyses. Hence we advocate further studies examining the role of host genetics and environmental variables on disease rates and vaccine responses among persons of various ethnicities.

In summary, our study showed that pneumococcal IgG responses both prior to and following revaccination with both the 23-valent polysaccharide and the 7-valent conjugate vaccine were similar among AA and Caucasian HIV-infected adults. These data suggest that environmental or other host factors, rather than differing antibody responses to vaccination, may underlie differential rates of pneumococcal disease reported among persons of different ethnic backgrounds.

Acknowledgements

The IDCRP HIV Working Group is comprised of: Susan Banks RN, Irma Barahona RN, CAPT Mary Bavaro MD, Carolyn Brandt RN, LCDR Helen Chun MD, Cathy Decker MD, Conner Eggleston, COL Susan Fraser, Heather Hairston, Josh Hartzell MD, Arthur Johnson MD, Alan Lifson MD MPH, Michelle Linfesty, Grace Macalino PhD, CAPT Jason Maguire MD, Scott Merritt, Christie Morse, MAJ Robert O’Connell MD, Cpt Jason Okulicz MD, Sheila Peel PhD, John Powers MD, CAPT (ret) Sybil Tasker MD, CDR Timothy Whitman MD, COL Glenn Wortmann MD, LTC Michael Zapor MD.

Support for this work (IDCRP RV-150) was provided by the Infectious Disease Clinical Research Program (IDCRP), a Department of Defense (DoD) program executed through the Uniformed Services University of the Health Sciences. This project has been funded in whole, or in part, with federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), under Inter-Agency Agreement Y1-AI-5072. Additional support was obtained from the Veterans Affairs Research Service.

Footnotes

The content of this publication is the sole responsibility of the authors and does not necessarily reflect the views or policies of the NIH or the Department of Health and Human Services, the DoD or the Departments of the Army, Navy or Air Force. Mention of trade names, commercial products, or organizations does not imply endorsement by the U.S. Government.

This work is original and has not been published elsewhere. Part of these data was presented at the XVIII International AIDS Conference, Vienna, Austria, July 18–23, 2010.

References

1. Janoff EN, Breiman RF, Daley CL, Hopewell PC. Pneumococcal disease during HIV infection. Epidemiologic, clinical, and immunologic perspectives. Ann Intern Med. 1992;117:314–324. [PubMed]
2. Gilks CF, Ojoo SA, Ojoo JC, Brindle RJ, Paul J, Batchelor BI, et al. Invasive pneumococcal disease in a cohort of predominantly HIV-1 infected female sex-workers in Nairobi, Kenya. Lancet. 1996;347:718–723. [PubMed]
3. Dworkin MS, Ward JW, Hanson DL, Jones JL, Kaplan JE. the Adult and Adolescent Spectrum of HIV Disease Project. Pneumococcal disease among human immunodeficiency virus-infected persons: incidence, risk factors, and impact of vaccination. Clin Infect Dis. 2001;32:794–800. [PubMed]
4. Barry PM, Zetola N, Keruly JC, Moore RD, Gebo KA, Lucas GM. Invasive pneumococcal disease in a cohort of HIV-infected adults: incidence and risk factors, 1990–2003. AIDS. 2006;20:437–444. [PubMed]
5. Frankel RE, Virata M, Hardalo C, Altice FL, Friedland G. Invasive pneumococcal disease: clinical features, serotypes, and antimicrobial resistance patterns in cases involving patients with and without human immunodeficiency virus infection. Clin Infect Dis. 1996;23:577–584. [PubMed]
6. Feikin DR, Feldman C, Schuchat A, Janoff EN. Global strategies to prevent bacterial pneumonia in adults with HIV disease. Lancet Infect Dis. 2004;4:445–455. [PubMed]
7. Redd SC, Rutherford GW, 3rd, Sande MA, Lifson AR, Hadley WK, Facklam RR, et al. The role of human immunodeficiency virus infection in pneumococcal bacteremia in San Francisco residents. J Infect Dis. 1990;162:1012–1017. [PubMed]
8. Bennett NM, Buffington J, LaForce FM. Pneumococcal bacteremia in Monroe County, New York. Am J Public Health. 1992;82:1513–1516. [PMC free article] [PubMed]
9. Breiman RF, Spika JS, Navarro VJ, Darden PM, Darby CP. Pneumococcal bacteremia in Charleston County, South Carolina. A decade later. Arch Intern Med. 1990;150:1401–1405. [PubMed]
10. Filice GA, Darby CP, Fraser DW. Pneumococcal bacteremia in Charleston County, South Carolina. Am J Epidemiol. 1980;112:828–835. [PubMed]
11. Gebo KA, Moore RD, Keruly JC, Chaisson RE. Risk factors for pneumococcal disease in human immunodeficiency virus-infected patients. J Infect Dis. 1996;173:857–862. [PubMed]
12. Breiman RF, Keller DW, Phelan MA, Sniadack DH, Stephens DS, Rimland D, et al. Evaluation of effectiveness of the 23-valent pneumococcal capsular polysaccharide vaccine for HIV-infected patients. Arch Intern Med. 2000;160:2633–2638. [PubMed]
13. French N, Nakiyingi J, Carpenter LM, Lugada E, Watera C, Moi K, et al. 23-valent pneumococcal polysaccharide vaccine in HIV-1-infected Ugandan adults: double-blind, randomised and placebo controlled trial. Lancet. 2000;355:2106–2111. [PubMed]
14. Crum-Cianflone NF, Huppler Hullsiek K, Roediger M, Ganesan A, Patel S, Landrum M, et al. and the Infectious Disease Clinical Research Program HIV Working Group. A Randomized Clinical Trial Comparing Revaccination with Pneumococcal Conjugate Vaccine (PCV) to Polysaccharide Vaccine (PPV) among HIV-Infected Adults. J Infect Dis. 2010 in press. [PMC free article] [PubMed]
15. Brodine SK, Shaffer RA, Starkey MJ, Tasker SA, Gilcrest JL, Louder MK, et al. Drug resistance patterns, genetic subtypes, clinical features, and risk factors in military personnel with HIV-1 seroconversion. Ann Intern Med. 1999;131:502–506. [PubMed]
16. Rubins JB, Alter M, Loch J, Janoff EN. Determination of antibody responses of elderly adults to all 23 capsular polysaccharides after pneumococcal vaccination. Infect Immun. 1999;67:5979–5984. [PMC free article] [PubMed]
17. Manoff SB, Liss C, Caulfield MJ, Marchese RD, Silber J, Boslego J, et al. Revaccination with a 23-valent pneumococcal polysaccharide vaccine induces elevated and persistent functional antibody responses in adults aged 65 > or = years. J Infect Dis. 2010;201:525–533. [PubMed]
18. Tasker SA, Wallace MR, Rubins JB, Paxton WB, O'Brien J, Janoff EN. Revaccination with 23-valent pneumococcal vaccine for patients infected with human immunodeficiency virus type 1: clinical, immunologic, and virologic responses. Clin Infect Dis. 2002;34:813–821. [PubMed]
19. Concepcion NF, Frasch CE. Pneumococcal type 22f polysaccharide absorption improves the specificity of a pneumococcal-polysaccharide enzyme-linked immunosorbent assay. Clin Diagn Lab Immunol. 2001;8:266–272. [PMC free article] [PubMed]
20. Training manual for enzyme linked immunosorbent assay for the quantitation of Streptococcus pneumoniae serotype specific IgG (Pn PS ELISA) [Accessed on March 1, 2010]. Available at : http://www.vaccine.uab.edu.
21. Centers for Disease Control and Prevention. Prevention of pneumococcal disease: recommendations of the Advisory Committee on Immunization Practices (ACIP) MMWR Recomm Rep. 1997;46:1–24. [PubMed]
22. French N, Gilks CF, Mujugira A, Fasching C, O'Brien J, Janoff EN. Pneumococcal vaccination in HIV-1-infected adults in Uganda: humoral response and two vaccine failures. AIDS. 1998;12:1683–1689. [PubMed]
23. French N, Moore M, Haikala R, Kayhty H, Gilks CF. A case-control study to investigate serological correlates of clinical failure of 23-valent pneumococcal polysaccharide vaccine in HIV-1-infected Ugandan adults. J Infect Dis. 2004;190:707–712. [PubMed]
24. French N, Gordon SB, Mwalukomo T, White SA, Mwafulirwa G, Longwe H, et al. A trial of a 7-valent pneumococcal conjugate vaccine in HIV-infected adults. N Engl J Med. 2010;362:812–822. [PMC free article] [PubMed]
25. Joint United Nations Programme on HIV/AIDS (UNAIDS) 2008 Report on the global AIDS epidemic. [Accessed June 18, 2010]. http://data.unaids.org/pub/GlobalReport/2008/JC1510_2008GlobalReport_en.zip.
26. Fenton KA. Changing epidemiology of HIV/AIDS in the United States: implications for enhancing and promoting HIV testing strategies. Clin Infect Dis. 2007;45:S213–S220. [PubMed]
27. Carson PJ, Schut RL, Simpson ML, O'Brien J, Janoff EN. Antibody class and subclass responses to pneumococcal polysaccharides following immunization of human immunodeficiency virus-infected patients. J Infect Dis. 1995;172:340–345. [PubMed]

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